Microfabricated fluorescence-activated cell sorter through hydrodynamic flow manipulation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-07

AUTHORS

Hyunwoo Bang, Chanil Chung, Jung Kyung Kim, Seong Hwan Kim, Seok Chung, Junha Park, Won Gu Lee, Hoyoung Yun, Joonmo Lee, Keunchang Cho, Dong-Chul Han, Jun Keun Chang

ABSTRACT

In this paper, we present a novel flow manipulation and signal detection scheme for a microfabricated fluorescence-activated cell sorter (μFACS). Optics setup was built on an inverted fluorescence microscope (IX71, Olympus, USA) and microchips were made of PDMS using soft lithography. Perfectly aligned axial illumination setup of the commercial microscope ensures effective signal detection. Hydrodynamic flow manipulation scheme is adopted for both flow focusing and cell sorting. Fast and robust sorting function is utilized by a nozzle flow from wide actuation channel. The sorting function is verified through real-time high-speed imaging. The captured images are used for adjusting delays between signal detection and sorting activation. The system automatically adjusts itself to any flow conditions, and so no adjustment is made to the flow parameters between runs. We believe that the proposed techniques will help breaking through the obstacles of μFACS system development. More... »

PAGES

746-753

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00542-005-0076-9

DOI

http://dx.doi.org/10.1007/s00542-005-0076-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042834285


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