Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-02

AUTHORS

A. Moscatelli, Giampiero Cai, Fabrizio Ciampolini, Mauro Cresti

ABSTRACT

It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles. More... »

PAGES

31-40

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004970050118

DOI

http://dx.doi.org/10.1007/s004970050118

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015916568


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT", 
          "id": "http://www.grid.ac/institutes/grid.9024.f", 
          "name": [
            "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moscatelli", 
        "givenName": "A.", 
        "id": "sg:person.0640051602.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640051602.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT", 
          "id": "http://www.grid.ac/institutes/grid.9024.f", 
          "name": [
            "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cai", 
        "givenName": "Giampiero", 
        "id": "sg:person.01055277110.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055277110.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT", 
          "id": "http://www.grid.ac/institutes/grid.9024.f", 
          "name": [
            "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ciampolini", 
        "givenName": "Fabrizio", 
        "id": "sg:person.015201267757.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015201267757.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT", 
          "id": "http://www.grid.ac/institutes/grid.9024.f", 
          "name": [
            "Dipartimento di Biologia Ambientale, Universit\u00e0 delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cresti", 
        "givenName": "Mauro", 
        "id": "sg:person.01143621702.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143621702.15"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1998-02", 
    "datePublishedReg": "1998-02-01", 
    "description": "Abstract\u2002It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton\u00d7100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s004970050118", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1023214", 
        "issn": [
          "2194-7953", 
          "2194-7961"
        ], 
        "name": "Plant Reproduction", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "keywords": [
      "heavy chain", 
      "immunological properties", 
      "Immunofluoresence studies", 
      "biochemical evidence", 
      "characteristic distribution pattern", 
      "synthetic peptides", 
      "cytoplasmic distribution", 
      "cell membrane", 
      "dynein heavy chain", 
      "antibodies", 
      "membranous organelles", 
      "molecular weight polypeptides", 
      "subcellular localization", 
      "association", 
      "membrane fraction", 
      "involvement", 
      "polypeptide", 
      "weight polypeptides", 
      "cells", 
      "agents", 
      "organelles", 
      "spot-like structures", 
      "peptides", 
      "high molecular weight polypeptides", 
      "evidence", 
      "tube", 
      "cytoplasmic targets", 
      "data", 
      "target", 
      "study", 
      "association of proteins", 
      "distribution patterns", 
      "protein", 
      "localization", 
      "presence", 
      "patterns", 
      "membrane", 
      "fraction", 
      "length", 
      "growth", 
      "Nicotiana tabacum pollen tubes", 
      "detergents", 
      "interaction", 
      "vegetative cells", 
      "pollen tubes", 
      "NaCl", 
      "pollen tube growth", 
      "loop", 
      "chain", 
      "tube growth", 
      "sequence", 
      "tube length", 
      "distribution", 
      "tabacum pollen tubes", 
      "doublet", 
      "Nicotiana", 
      "properties", 
      "band", 
      "structure", 
      "paper", 
      "Na2CO3", 
      "dynein heavy chain-related polypeptides", 
      "heavy chain-related polypeptides", 
      "chain-related polypeptides", 
      "dynein heavy chain-related bands", 
      "heavy chain-related bands", 
      "chain-related bands", 
      "pollen tube membrane fraction", 
      "tube membrane fraction", 
      "dynein heavy chain-related doublet", 
      "heavy chain-related doublet", 
      "chain-related doublet"
    ], 
    "name": "Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum", 
    "pagination": "31-40", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1015916568"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s004970050118"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s004970050118", 
      "https://app.dimensions.ai/details/publication/pub.1015916568"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:09", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_296.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s004970050118"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s004970050118'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s004970050118'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s004970050118'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s004970050118'


 

This table displays all metadata directly associated to this object as RDF triples.

151 TRIPLES      21 PREDICATES      98 URIs      90 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s004970050118 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N3dc2df166c4341c48ed35451c241d4d1
4 schema:datePublished 1998-02
5 schema:datePublishedReg 1998-02-01
6 schema:description Abstract It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles.
7 schema:genre article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N7d2e461530284f3db0ae3e10b6e68222
11 Nbfc744dcbea84824b7c9473d8426f968
12 sg:journal.1023214
13 schema:keywords Immunofluoresence studies
14 Na2CO3
15 NaCl
16 Nicotiana
17 Nicotiana tabacum pollen tubes
18 agents
19 antibodies
20 association
21 association of proteins
22 band
23 biochemical evidence
24 cell membrane
25 cells
26 chain
27 chain-related bands
28 chain-related doublet
29 chain-related polypeptides
30 characteristic distribution pattern
31 cytoplasmic distribution
32 cytoplasmic targets
33 data
34 detergents
35 distribution
36 distribution patterns
37 doublet
38 dynein heavy chain
39 dynein heavy chain-related bands
40 dynein heavy chain-related doublet
41 dynein heavy chain-related polypeptides
42 evidence
43 fraction
44 growth
45 heavy chain
46 heavy chain-related bands
47 heavy chain-related doublet
48 heavy chain-related polypeptides
49 high molecular weight polypeptides
50 immunological properties
51 interaction
52 involvement
53 length
54 localization
55 loop
56 membrane
57 membrane fraction
58 membranous organelles
59 molecular weight polypeptides
60 organelles
61 paper
62 patterns
63 peptides
64 pollen tube growth
65 pollen tube membrane fraction
66 pollen tubes
67 polypeptide
68 presence
69 properties
70 protein
71 sequence
72 spot-like structures
73 structure
74 study
75 subcellular localization
76 synthetic peptides
77 tabacum pollen tubes
78 target
79 tube
80 tube growth
81 tube length
82 tube membrane fraction
83 vegetative cells
84 weight polypeptides
85 schema:name Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum
86 schema:pagination 31-40
87 schema:productId N6d22bf3eb3b64c0b96679888e51e3fd7
88 Nc5c6c90a9a7945cb808af65e7b1cc7a2
89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015916568
90 https://doi.org/10.1007/s004970050118
91 schema:sdDatePublished 2022-01-01T18:09
92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
93 schema:sdPublisher N8a1fe474c1774091b4902aee70e2e9c8
94 schema:url https://doi.org/10.1007/s004970050118
95 sgo:license sg:explorer/license/
96 sgo:sdDataset articles
97 rdf:type schema:ScholarlyArticle
98 N25631e7aea1c4e128d722c5f6b13a97a rdf:first sg:person.01143621702.15
99 rdf:rest rdf:nil
100 N3dc2df166c4341c48ed35451c241d4d1 rdf:first sg:person.0640051602.44
101 rdf:rest N4c508451ba4c4a56ab4c7c8a59a035f7
102 N4c508451ba4c4a56ab4c7c8a59a035f7 rdf:first sg:person.01055277110.37
103 rdf:rest N684503ec6aca4925b843f88d5d76a064
104 N684503ec6aca4925b843f88d5d76a064 rdf:first sg:person.015201267757.99
105 rdf:rest N25631e7aea1c4e128d722c5f6b13a97a
106 N6d22bf3eb3b64c0b96679888e51e3fd7 schema:name doi
107 schema:value 10.1007/s004970050118
108 rdf:type schema:PropertyValue
109 N7d2e461530284f3db0ae3e10b6e68222 schema:issueNumber 1
110 rdf:type schema:PublicationIssue
111 N8a1fe474c1774091b4902aee70e2e9c8 schema:name Springer Nature - SN SciGraph project
112 rdf:type schema:Organization
113 Nbfc744dcbea84824b7c9473d8426f968 schema:volumeNumber 11
114 rdf:type schema:PublicationVolume
115 Nc5c6c90a9a7945cb808af65e7b1cc7a2 schema:name dimensions_id
116 schema:value pub.1015916568
117 rdf:type schema:PropertyValue
118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biological Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biochemistry and Cell Biology
123 rdf:type schema:DefinedTerm
124 sg:journal.1023214 schema:issn 2194-7953
125 2194-7961
126 schema:name Plant Reproduction
127 schema:publisher Springer Nature
128 rdf:type schema:Periodical
129 sg:person.01055277110.37 schema:affiliation grid-institutes:grid.9024.f
130 schema:familyName Cai
131 schema:givenName Giampiero
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055277110.37
133 rdf:type schema:Person
134 sg:person.01143621702.15 schema:affiliation grid-institutes:grid.9024.f
135 schema:familyName Cresti
136 schema:givenName Mauro
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143621702.15
138 rdf:type schema:Person
139 sg:person.015201267757.99 schema:affiliation grid-institutes:grid.9024.f
140 schema:familyName Ciampolini
141 schema:givenName Fabrizio
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015201267757.99
143 rdf:type schema:Person
144 sg:person.0640051602.44 schema:affiliation grid-institutes:grid.9024.f
145 schema:familyName Moscatelli
146 schema:givenName A.
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640051602.44
148 rdf:type schema:Person
149 grid-institutes:grid.9024.f schema:alternateName Dipartimento di Biologia Ambientale, Università delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT
150 schema:name Dipartimento di Biologia Ambientale, Università delgi Studi di Siena, Via P.A. Mattioli 4, 53100 Siena, Italy Tel. +39-577-298856; Fax +39-577-298860 e-mail: moscatelli@unisi.it, IT
151 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...