Toward in vitro fertilization in Brachiaria spp. View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-09

AUTHORS

Diva M. A. Dusi, Elizangela R. Alves, Michiel T. M. Willemse, Rosana Falcão, Cacilda B. do Valle, Vera T. C. Carneiro

ABSTRACT

Brachiaria are forage grasses widely cultivated in tropical areas. In vitro pollination was applied to accessions of Brachiaria spp. by placing pollen of non-dehiscent anthers on a solid medium near isolated ovaries. Viability and in vitro germination were tested in order to establish good conditions for pollen development. Comparing sexual to apomictic plants, apomictic pollen has more abortion after meiosis during the microspore stage and a lower viability and, of both types, only some plants have sufficient germination in a high sugar concentration. Using in vitro pollination with the sexual plant, the pollen tube penetrates into the nucellus and micropyle, but the embryo sac degenerates and collapses. In the apomictic B. decumbens, in vitro pollination leads to the transfer of the sperm nuclei into the egg cell and the central cell. The results are discussed according to normal fertilization and barriers in sexual and apomictic plants. More... »

PAGES

187-197

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00497-010-0134-z

DOI

http://dx.doi.org/10.1007/s00497-010-0134-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020652402

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20135327


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