Identification of two genes encoding microsomal oleate desaturases (FAD2) from the biodiesel plant Pongamia pinnata L. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-08

AUTHORS

Ramesh Aadi Moolam, Anuma Singh, Rahul G. Shelke, Paul T. Scott, Peter M. Gresshoff, Latha Rangan

ABSTRACT

The current study dissect microsomal oleate desaturase genes having differential expression pattern with respect to temperature from the seeds ofPongamia pinnata,and are grouped with other legumes and biofuel plants. Biofuel often is available as plant oil or products derived thereafter, such as biodiesel. In view of the anticipated fossil fuel shortage, the biochemical and genetic basis of vegetable oil biosynthesis is vital. We are focusing on the versatile oil-yielding legume tree Pongamia pinnata. Microsomal oleate desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in non-photosynthetic tissues. This work reports on the isolation of two full length cDNA clones, tissue expression and copy number detection of Pongamia FAD2 genes, and also on the transcriptional regulation at low and high temperatures. The deduced amino acid sequences of both PpFAD2 proteins share 83 % identity and display three typical histidine boxes that are characteristics of all membrane-bound microsomal oleate desaturases. Both sequences possess aromatic amino acid containing sequence motifs at the C-terminal end necessary for maintaining endoplasmic reticulum (ER) localization. Southern blot analysis is consistent with the presence of at least two copies of FAD2 in the pongamia genome. Quantitative real-time PCR analysis showed that PpFAD2-1 is expressed strongly in developing seeds and, showing very low levels of expression in vegetative tissues, whereas PpFAD2-2 is constitutively expressed in both vegetative tissues and the developing seeds, with higher transcript levels in roots, stems and leaves. In response to low temperature stress PpFAD2-1 and PpFAD2-2 were differentially expressed in various tissues. The PpFAD2-2 transcript dramatically decreased in roots, stems and leaves under low temperatures, whereas PpFAD2-1 showed a significant increase in these tissues. More... »

PAGES

1351-1360

References to SciGraph publications

  • 2001-09. Spatial and temporal regulation of three different microsomal oleate desaturase genes (FAD2) from normal-type and high-oleic varieties of sunflower (Helianthus annuus L.) in MOLECULAR BREEDING
  • 2005-10. Storage lipid accumulation and acyltransferase action in developing flaxseed in LIPIDS
  • 2012-12. Oil, Fatty Acid Profile and Karanjin Content in Developing Pongamia pinnata (L.) Pierre Seeds in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 2009-07. Molecular cloning and expression analysis of three omega-6 desaturase genes from purslane (Portulaca oleracea L.) in BIOTECHNOLOGY LETTERS
  • 2000-11. Isolation and characterization of the Δ12-fatty acid desaturase in peanut (Arachis hypogaea L.) and search for polymorphisms for the high oleate trait in Spanish market-type lines in THEORETICAL AND APPLIED GENETICS
  • 2012-02. Molecular Cloning and Expression Analysis of Genes Encoding Two Microsomal Oleate Desaturases (FAD2) from Safflower (Carthamus tinctorius L.) in PLANT MOLECULAR BIOLOGY REPORTER
  • 2001-12. Changes in fatty acids composition during seed growth and physicochemical characteristics of oil extracted from four safflower cultivars in PLANT FOODS FOR HUMAN NUTRITION
  • 1997-10. Fatty acid distribution and lipid metabolism in developing seeds of laurate-producing rape (Brassica napus L.) in PLANTA
  • 2006-08. Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.) in THEORETICAL AND APPLIED GENETICS
  • 2008-03. Pongamia pinnata: An Untapped Resource for the Biofuels Industry of the Future in BIOENERGY RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00468-016-1371-z

    DOI

    http://dx.doi.org/10.1007/s00468-016-1371-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012128412


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Indian Institute of Technology Guwahati", 
              "id": "https://www.grid.ac/institutes/grid.417972.e", 
              "name": [
                "Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781 039, Guwahati, Assam, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Aadi Moolam", 
            "givenName": "Ramesh", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Indian Institute of Technology Guwahati", 
              "id": "https://www.grid.ac/institutes/grid.417972.e", 
              "name": [
                "Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781 039, Guwahati, Assam, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Singh", 
            "givenName": "Anuma", 
            "id": "sg:person.01346621213.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346621213.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Indian Institute of Technology Guwahati", 
              "id": "https://www.grid.ac/institutes/grid.417972.e", 
              "name": [
                "Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781 039, Guwahati, Assam, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shelke", 
            "givenName": "Rahul G.", 
            "id": "sg:person.010175642002.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010175642002.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Queensland", 
              "id": "https://www.grid.ac/institutes/grid.1003.2", 
              "name": [
                "Centre for Integrative Legume Research (CILR), The University of Queensland, 4072, St. Lucia, Brisbane, QLD, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Scott", 
            "givenName": "Paul T.", 
            "id": "sg:person.01161353460.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161353460.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Queensland", 
              "id": "https://www.grid.ac/institutes/grid.1003.2", 
              "name": [
                "Centre for Integrative Legume Research (CILR), The University of Queensland, 4072, St. Lucia, Brisbane, QLD, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gresshoff", 
            "givenName": "Peter M.", 
            "id": "sg:person.01363624355.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363624355.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Indian Institute of Technology Guwahati", 
              "id": "https://www.grid.ac/institutes/grid.417972.e", 
              "name": [
                "Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781 039, Guwahati, Assam, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rangan", 
            "givenName": "Latha", 
            "id": "sg:person.016122764203.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016122764203.20"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.renene.2014.02.037", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000875338"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-2836(82)90515-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001452096"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/molbev/msm092", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001758106"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-009-9956-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002684685", 
              "https://doi.org/10.1007/s10529-009-9956-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-009-9956-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002684685", 
              "https://doi.org/10.1007/s10529-009-9956-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-009-9956-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002684685", 
              "https://doi.org/10.1007/s10529-009-9956-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11745-005-1467-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004692803", 
              "https://doi.org/10.1007/s11745-005-1467-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s2095-3119(13)60473-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006202769"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.arplant.52.1.335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006945908"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.110.1.311", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009847770"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/02648725.1987.10647839", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010215250"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(87)90021-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013321498"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkn180", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014918820"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.arplant.49.1.611", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015443689"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001220051589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016429922", 
              "https://doi.org/10.1007/s001220051589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001220051589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016429922", 
              "https://doi.org/10.1007/s001220051589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/14.4.378", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016482060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1013324329322", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016821116", 
              "https://doi.org/10.1023/a:1013324329322"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biombioe.2009.08.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021000427"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004250050200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022533875", 
              "https://doi.org/10.1007/s004250050200"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(99)01429-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023765219"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(02)02250-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024497381"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11105-011-0322-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024876529", 
              "https://doi.org/10.1007/s11105-011-0322-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1011860810082", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025684395", 
              "https://doi.org/10.1023/a:1011860810082"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biombioe.2009.10.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026315399"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jxb/ern065", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026751262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.6.1.147", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027299386"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.106.4.1453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029323547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2005.02535.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029657435"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2005.02535.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029657435"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jplph.2013.04.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029729976"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0005-2760(98)00091-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031983820"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phytochem.2010.11.026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032481483"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.006353", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036405738"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/dvg.1020110108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037255553"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/dvg.1020110108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037255553"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-006-0315-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038913877", 
              "https://doi.org/10.1007/s00122-006-0315-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-006-0315-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038913877", 
              "https://doi.org/10.1007/s00122-006-0315-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.gene.2014.03.047", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039477239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biombioe.2012.03.029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040701095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12155-008-9003-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040994980", 
              "https://doi.org/10.1007/s12155-008-9003-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/25.24.4876", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041279110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.arplant.48.1.109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042844682"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1071/fp08276", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044732230"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1139/b92-021", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045039444"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/25.17.3389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047265454"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11746-012-2126-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047334692", 
              "https://doi.org/10.1007/s11746-012-2126-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.122.2.389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050067781"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.7.7.957", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050514470"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2008.03442.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050890704"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phytochem.2005.04.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052074353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phytochem.2005.04.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052074353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jplph.2006.08.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053116000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2004.01949.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053512901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00209a009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055165323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2135/cropsci2006.06.0382tpg", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069030030"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3835/plantgenome2013.05.0015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1071447850"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-08", 
        "datePublishedReg": "2016-08-01", 
        "description": "The current study dissect microsomal oleate desaturase genes having differential expression pattern with respect to temperature from the seeds ofPongamia pinnata,and are grouped with other legumes and biofuel plants. Biofuel often is available as plant oil or products derived thereafter, such as biodiesel. In view of the anticipated fossil fuel shortage, the biochemical and genetic basis of vegetable oil biosynthesis is vital. We are focusing on the versatile oil-yielding legume tree Pongamia pinnata. Microsomal oleate desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in non-photosynthetic tissues. This work reports on the isolation of two full length cDNA clones, tissue expression and copy number detection of Pongamia FAD2 genes, and also on the transcriptional regulation at low and high temperatures. The deduced amino acid sequences of both PpFAD2 proteins share 83 % identity and display three typical histidine boxes that are characteristics of all membrane-bound microsomal oleate desaturases. Both sequences possess aromatic amino acid containing sequence motifs at the C-terminal end necessary for maintaining endoplasmic reticulum (ER) localization. Southern blot analysis is consistent with the presence of at least two copies of FAD2 in the pongamia genome. Quantitative real-time PCR analysis showed that PpFAD2-1 is expressed strongly in developing seeds and, showing very low levels of expression in vegetative tissues, whereas PpFAD2-2 is constitutively expressed in both vegetative tissues and the developing seeds, with higher transcript levels in roots, stems and leaves. In response to low temperature stress PpFAD2-1 and PpFAD2-2 were differentially expressed in various tissues. The PpFAD2-2 transcript dramatically decreased in roots, stems and leaves under low temperatures, whereas PpFAD2-1 showed a significant increase in these tissues.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00468-016-1371-z", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1023155", 
            "issn": [
              "0931-1890", 
              "1432-2285"
            ], 
            "name": "Trees", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "30"
          }
        ], 
        "name": "Identification of two genes encoding microsomal oleate desaturases (FAD2) from the biodiesel plant Pongamia pinnata L.", 
        "pagination": "1351-1360", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "39c87ac4ff630fcc064c74fb06cb075e107189a280c9e667524390690954ed42"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00468-016-1371-z"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1012128412"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00468-016-1371-z", 
          "https://app.dimensions.ai/details/publication/pub.1012128412"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T15:00", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000511.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs00468-016-1371-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00468-016-1371-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00468-016-1371-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00468-016-1371-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00468-016-1371-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    258 TRIPLES      21 PREDICATES      77 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00468-016-1371-z schema:about anzsrc-for:06
    2 anzsrc-for:0607
    3 schema:author N6c14c3ba4a5e49f08345da46de5491ce
    4 schema:citation sg:pub.10.1007/s00122-006-0315-1
    5 sg:pub.10.1007/s001220051589
    6 sg:pub.10.1007/s004250050200
    7 sg:pub.10.1007/s10529-009-9956-x
    8 sg:pub.10.1007/s11105-011-0322-5
    9 sg:pub.10.1007/s11745-005-1467-0
    10 sg:pub.10.1007/s11746-012-2126-7
    11 sg:pub.10.1007/s12155-008-9003-0
    12 sg:pub.10.1023/a:1011860810082
    13 sg:pub.10.1023/a:1013324329322
    14 https://doi.org/10.1002/dvg.1020110108
    15 https://doi.org/10.1016/0003-2697(87)90021-2
    16 https://doi.org/10.1016/0022-2836(82)90515-0
    17 https://doi.org/10.1016/j.biombioe.2009.08.005
    18 https://doi.org/10.1016/j.biombioe.2009.10.006
    19 https://doi.org/10.1016/j.biombioe.2012.03.029
    20 https://doi.org/10.1016/j.gene.2014.03.047
    21 https://doi.org/10.1016/j.jplph.2006.08.007
    22 https://doi.org/10.1016/j.jplph.2013.04.019
    23 https://doi.org/10.1016/j.phytochem.2005.04.004
    24 https://doi.org/10.1016/j.phytochem.2010.11.026
    25 https://doi.org/10.1016/j.renene.2014.02.037
    26 https://doi.org/10.1016/s0005-2760(98)00091-5
    27 https://doi.org/10.1016/s1360-1385(02)02250-1
    28 https://doi.org/10.1016/s1360-1385(99)01429-6
    29 https://doi.org/10.1016/s2095-3119(13)60473-6
    30 https://doi.org/10.1021/bi00209a009
    31 https://doi.org/10.1071/fp08276
    32 https://doi.org/10.1080/02648725.1987.10647839
    33 https://doi.org/10.1093/bioinformatics/14.4.378
    34 https://doi.org/10.1093/jxb/ern065
    35 https://doi.org/10.1093/molbev/msm092
    36 https://doi.org/10.1093/nar/25.17.3389
    37 https://doi.org/10.1093/nar/25.24.4876
    38 https://doi.org/10.1093/nar/gkn180
    39 https://doi.org/10.1104/pp.006353
    40 https://doi.org/10.1104/pp.106.4.1453
    41 https://doi.org/10.1104/pp.110.1.311
    42 https://doi.org/10.1104/pp.122.2.389
    43 https://doi.org/10.1105/tpc.6.1.147
    44 https://doi.org/10.1105/tpc.7.7.957
    45 https://doi.org/10.1111/j.1365-313x.2004.01949.x
    46 https://doi.org/10.1111/j.1365-313x.2005.02535.x
    47 https://doi.org/10.1111/j.1365-313x.2008.03442.x
    48 https://doi.org/10.1139/b92-021
    49 https://doi.org/10.1146/annurev.arplant.48.1.109
    50 https://doi.org/10.1146/annurev.arplant.49.1.611
    51 https://doi.org/10.1146/annurev.arplant.52.1.335
    52 https://doi.org/10.2135/cropsci2006.06.0382tpg
    53 https://doi.org/10.3835/plantgenome2013.05.0015
    54 schema:datePublished 2016-08
    55 schema:datePublishedReg 2016-08-01
    56 schema:description The current study dissect microsomal oleate desaturase genes having differential expression pattern with respect to temperature from the seeds ofPongamia pinnata,and are grouped with other legumes and biofuel plants. Biofuel often is available as plant oil or products derived thereafter, such as biodiesel. In view of the anticipated fossil fuel shortage, the biochemical and genetic basis of vegetable oil biosynthesis is vital. We are focusing on the versatile oil-yielding legume tree Pongamia pinnata. Microsomal oleate desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in non-photosynthetic tissues. This work reports on the isolation of two full length cDNA clones, tissue expression and copy number detection of Pongamia FAD2 genes, and also on the transcriptional regulation at low and high temperatures. The deduced amino acid sequences of both PpFAD2 proteins share 83 % identity and display three typical histidine boxes that are characteristics of all membrane-bound microsomal oleate desaturases. Both sequences possess aromatic amino acid containing sequence motifs at the C-terminal end necessary for maintaining endoplasmic reticulum (ER) localization. Southern blot analysis is consistent with the presence of at least two copies of FAD2 in the pongamia genome. Quantitative real-time PCR analysis showed that PpFAD2-1 is expressed strongly in developing seeds and, showing very low levels of expression in vegetative tissues, whereas PpFAD2-2 is constitutively expressed in both vegetative tissues and the developing seeds, with higher transcript levels in roots, stems and leaves. In response to low temperature stress PpFAD2-1 and PpFAD2-2 were differentially expressed in various tissues. The PpFAD2-2 transcript dramatically decreased in roots, stems and leaves under low temperatures, whereas PpFAD2-1 showed a significant increase in these tissues.
    57 schema:genre research_article
    58 schema:inLanguage en
    59 schema:isAccessibleForFree false
    60 schema:isPartOf N0b7e0c6a5b2c44aa8e138026b5284bad
    61 N693a13a5c2f14c9f84f48ca99dddaa77
    62 sg:journal.1023155
    63 schema:name Identification of two genes encoding microsomal oleate desaturases (FAD2) from the biodiesel plant Pongamia pinnata L.
    64 schema:pagination 1351-1360
    65 schema:productId N70a3ba031f114f8d814047f98e3bf157
    66 Nb9e2b65fb9b9433eb97043472c34b695
    67 Nfb57569d8fc1415fb8df737773870fa1
    68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012128412
    69 https://doi.org/10.1007/s00468-016-1371-z
    70 schema:sdDatePublished 2019-04-10T15:00
    71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    72 schema:sdPublisher N6384f1dbba4c41ca85fa3ed2342f4052
    73 schema:url http://link.springer.com/10.1007%2Fs00468-016-1371-z
    74 sgo:license sg:explorer/license/
    75 sgo:sdDataset articles
    76 rdf:type schema:ScholarlyArticle
    77 N06f2910840004076aab459a15eb106ad schema:affiliation https://www.grid.ac/institutes/grid.417972.e
    78 schema:familyName Aadi Moolam
    79 schema:givenName Ramesh
    80 rdf:type schema:Person
    81 N0b7e0c6a5b2c44aa8e138026b5284bad schema:issueNumber 4
    82 rdf:type schema:PublicationIssue
    83 N23fda82035d94df2abf9f6017e3e6e5d rdf:first sg:person.01363624355.34
    84 rdf:rest Nea8e625309494e6c9314263ab95d4dfe
    85 N2fc67acb4cae45b3880c88f19cc3f801 rdf:first sg:person.01161353460.45
    86 rdf:rest N23fda82035d94df2abf9f6017e3e6e5d
    87 N440dc63e72f843bbad2fed04de88d502 rdf:first sg:person.01346621213.51
    88 rdf:rest N77f0a994a6124b92b21ca35daf7d1ecc
    89 N6384f1dbba4c41ca85fa3ed2342f4052 schema:name Springer Nature - SN SciGraph project
    90 rdf:type schema:Organization
    91 N693a13a5c2f14c9f84f48ca99dddaa77 schema:volumeNumber 30
    92 rdf:type schema:PublicationVolume
    93 N6c14c3ba4a5e49f08345da46de5491ce rdf:first N06f2910840004076aab459a15eb106ad
    94 rdf:rest N440dc63e72f843bbad2fed04de88d502
    95 N70a3ba031f114f8d814047f98e3bf157 schema:name readcube_id
    96 schema:value 39c87ac4ff630fcc064c74fb06cb075e107189a280c9e667524390690954ed42
    97 rdf:type schema:PropertyValue
    98 N77f0a994a6124b92b21ca35daf7d1ecc rdf:first sg:person.010175642002.02
    99 rdf:rest N2fc67acb4cae45b3880c88f19cc3f801
    100 Nb9e2b65fb9b9433eb97043472c34b695 schema:name doi
    101 schema:value 10.1007/s00468-016-1371-z
    102 rdf:type schema:PropertyValue
    103 Nea8e625309494e6c9314263ab95d4dfe rdf:first sg:person.016122764203.20
    104 rdf:rest rdf:nil
    105 Nfb57569d8fc1415fb8df737773870fa1 schema:name dimensions_id
    106 schema:value pub.1012128412
    107 rdf:type schema:PropertyValue
    108 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    109 schema:name Biological Sciences
    110 rdf:type schema:DefinedTerm
    111 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    112 schema:name Plant Biology
    113 rdf:type schema:DefinedTerm
    114 sg:journal.1023155 schema:issn 0931-1890
    115 1432-2285
    116 schema:name Trees
    117 rdf:type schema:Periodical
    118 sg:person.010175642002.02 schema:affiliation https://www.grid.ac/institutes/grid.417972.e
    119 schema:familyName Shelke
    120 schema:givenName Rahul G.
    121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010175642002.02
    122 rdf:type schema:Person
    123 sg:person.01161353460.45 schema:affiliation https://www.grid.ac/institutes/grid.1003.2
    124 schema:familyName Scott
    125 schema:givenName Paul T.
    126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01161353460.45
    127 rdf:type schema:Person
    128 sg:person.01346621213.51 schema:affiliation https://www.grid.ac/institutes/grid.417972.e
    129 schema:familyName Singh
    130 schema:givenName Anuma
    131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346621213.51
    132 rdf:type schema:Person
    133 sg:person.01363624355.34 schema:affiliation https://www.grid.ac/institutes/grid.1003.2
    134 schema:familyName Gresshoff
    135 schema:givenName Peter M.
    136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363624355.34
    137 rdf:type schema:Person
    138 sg:person.016122764203.20 schema:affiliation https://www.grid.ac/institutes/grid.417972.e
    139 schema:familyName Rangan
    140 schema:givenName Latha
    141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016122764203.20
    142 rdf:type schema:Person
    143 sg:pub.10.1007/s00122-006-0315-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038913877
    144 https://doi.org/10.1007/s00122-006-0315-1
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/s001220051589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016429922
    147 https://doi.org/10.1007/s001220051589
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1007/s004250050200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022533875
    150 https://doi.org/10.1007/s004250050200
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1007/s10529-009-9956-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002684685
    153 https://doi.org/10.1007/s10529-009-9956-x
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1007/s11105-011-0322-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024876529
    156 https://doi.org/10.1007/s11105-011-0322-5
    157 rdf:type schema:CreativeWork
    158 sg:pub.10.1007/s11745-005-1467-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004692803
    159 https://doi.org/10.1007/s11745-005-1467-0
    160 rdf:type schema:CreativeWork
    161 sg:pub.10.1007/s11746-012-2126-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047334692
    162 https://doi.org/10.1007/s11746-012-2126-7
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1007/s12155-008-9003-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040994980
    165 https://doi.org/10.1007/s12155-008-9003-0
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1023/a:1011860810082 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025684395
    168 https://doi.org/10.1023/a:1011860810082
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1023/a:1013324329322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016821116
    171 https://doi.org/10.1023/a:1013324329322
    172 rdf:type schema:CreativeWork
    173 https://doi.org/10.1002/dvg.1020110108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037255553
    174 rdf:type schema:CreativeWork
    175 https://doi.org/10.1016/0003-2697(87)90021-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013321498
    176 rdf:type schema:CreativeWork
    177 https://doi.org/10.1016/0022-2836(82)90515-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001452096
    178 rdf:type schema:CreativeWork
    179 https://doi.org/10.1016/j.biombioe.2009.08.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021000427
    180 rdf:type schema:CreativeWork
    181 https://doi.org/10.1016/j.biombioe.2009.10.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026315399
    182 rdf:type schema:CreativeWork
    183 https://doi.org/10.1016/j.biombioe.2012.03.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040701095
    184 rdf:type schema:CreativeWork
    185 https://doi.org/10.1016/j.gene.2014.03.047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039477239
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1016/j.jplph.2006.08.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053116000
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.1016/j.jplph.2013.04.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029729976
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1016/j.phytochem.2005.04.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052074353
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1016/j.phytochem.2010.11.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032481483
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1016/j.renene.2014.02.037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000875338
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1016/s0005-2760(98)00091-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031983820
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1016/s1360-1385(02)02250-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024497381
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1016/s1360-1385(99)01429-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023765219
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1016/s2095-3119(13)60473-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006202769
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1021/bi00209a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055165323
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1071/fp08276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044732230
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1080/02648725.1987.10647839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010215250
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1093/bioinformatics/14.4.378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016482060
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1093/jxb/ern065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026751262
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1093/molbev/msm092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001758106
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1093/nar/25.17.3389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047265454
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1093/nar/25.24.4876 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041279110
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1093/nar/gkn180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014918820
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1104/pp.006353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036405738
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1104/pp.106.4.1453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029323547
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1104/pp.110.1.311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009847770
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1104/pp.122.2.389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050067781
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1105/tpc.6.1.147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027299386
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1105/tpc.7.7.957 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050514470
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1111/j.1365-313x.2004.01949.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053512901
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1111/j.1365-313x.2005.02535.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029657435
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1111/j.1365-313x.2008.03442.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050890704
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1139/b92-021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045039444
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1146/annurev.arplant.48.1.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042844682
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1146/annurev.arplant.49.1.611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015443689
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1146/annurev.arplant.52.1.335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006945908
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.2135/cropsci2006.06.0382tpg schema:sameAs https://app.dimensions.ai/details/publication/pub.1069030030
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.3835/plantgenome2013.05.0015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071447850
    252 rdf:type schema:CreativeWork
    253 https://www.grid.ac/institutes/grid.1003.2 schema:alternateName University of Queensland
    254 schema:name Centre for Integrative Legume Research (CILR), The University of Queensland, 4072, St. Lucia, Brisbane, QLD, Australia
    255 rdf:type schema:Organization
    256 https://www.grid.ac/institutes/grid.417972.e schema:alternateName Indian Institute of Technology Guwahati
    257 schema:name Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781 039, Guwahati, Assam, India
    258 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...