Molecular cloning, characterization, and functional analysis of CcBBM gene from camphor tree (Cinnamomum camphora L.) View Full Text


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Article Info

DATE

2016-08

AUTHORS

Qinhong Liu, Cuijie Zhang, Yihui Cheng, Bo Zheng, Manzhu Bao, Xueping Shi

ABSTRACT

Cloning and functional analysis of CcBBM gene. Baby boom (BBM), as a transcription factor of AP2/ERF family, has great potence in promoting somatic embryogenesis. In this study, a BBM gene from somatic embryos of camphor tree (Cinnamomum camphora L.), named CcBBM, was cloned and characterized. A partial fragment of 560 bp of CcBBM was amplified by degenerate PCR based on the two conserved AP2 domains of other annotated BBMs. Full-length cDNA of CcBBM was obtained by a cloning strategy combing degenerate PCR and RACE technique. The open-reading frame of CcBBM is 2169 bp in length, which encodes 722 amino acids. A comparison of the deduced amino acid sequence of CcBBM with BBM from other species showed high similarities, ranging from 51 to 80 %. The mRNA levels of CcBBM in different tissues were detected by semi-quantitative reverse transcription PCR (RT-PCR). CcBBM was expressed in most tissues, with highest expression level in somatic embryos, followed by roots, young fruits and flowers. Ectopic expression of CcBBM gene in Arabidopsis exhibited a variety of deformed and variegated leaves and short roots. No spontaneous somatic embryogenesis was observed in 35S::CcBBM transgenic plants. These results will help us understand the role of CcBBM in somatic embryogenesis in camphor tree. More... »

PAGES

1033-1043

References to SciGraph publications

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  • 2009-10. Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium in TREES
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  • 2005-03. The effect of aminoglycoside antibiotics on the adventitious regeneration from apricot leaves and selection of nptII-transformed leaf tissues in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2011-06. Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor in PLANT CELL REPORTS
  • 2003-08. Micropropagation of camphor tree (Cinnamomum camphora) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2010-04. Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
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    33 schema:description Cloning and functional analysis of CcBBM gene. Baby boom (BBM), as a transcription factor of AP2/ERF family, has great potence in promoting somatic embryogenesis. In this study, a BBM gene from somatic embryos of camphor tree (Cinnamomum camphora L.), named CcBBM, was cloned and characterized. A partial fragment of 560 bp of CcBBM was amplified by degenerate PCR based on the two conserved AP2 domains of other annotated BBMs. Full-length cDNA of CcBBM was obtained by a cloning strategy combing degenerate PCR and RACE technique. The open-reading frame of CcBBM is 2169 bp in length, which encodes 722 amino acids. A comparison of the deduced amino acid sequence of CcBBM with BBM from other species showed high similarities, ranging from 51 to 80 %. The mRNA levels of CcBBM in different tissues were detected by semi-quantitative reverse transcription PCR (RT-PCR). CcBBM was expressed in most tissues, with highest expression level in somatic embryos, followed by roots, young fruits and flowers. Ectopic expression of CcBBM gene in Arabidopsis exhibited a variety of deformed and variegated leaves and short roots. No spontaneous somatic embryogenesis was observed in 35S::CcBBM transgenic plants. These results will help us understand the role of CcBBM in somatic embryogenesis in camphor tree.
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