Transformation of intact cells of Saccharomyces cerevisiae by square wave pulses using castellated microelectrodes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-04

AUTHORS

G. L. Prasanna, T. Panda, P. Prabhakar Rao

ABSTRACT

Genetic transformation of intact cells of Saccharomyces cerevisiae with an expression vector pYES 2, to efficiencies of 105 to 106 by high voltage electroporation is presented. Prototrophic transformants of yeast expressing resistance to ampicillin were obtained by subjecting the mixture of cells and DNA to a single square wave pulse at an amplitude of 2.5, 2.75 and 3.0 kV/cm in combination with a pulse width of 4, 5 and 3 msec for the three different strains Y915, Y742 and INVSC 1 respectively. The critical factors and electrical parameters which determine the transformation efficiency were examined. This communication describes the optimal conditions for reproducible and high efficiency transformation of yeast by the method of electroporation. More... »

PAGES

265-268

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004490050320

DOI

http://dx.doi.org/10.1007/s004490050320

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005284311


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