Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high ... View Full Text


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Article Info

DATE

2021-04-08

AUTHORS

Li-Qun Jin, Xian-Xiao Chen, Yi-Ting Jin, Jun-Kang Shentu, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

To improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn Syrup (HFCS) in a recirculating packed bed reactor for 603 h at a constant flow rate. It showed that the immobilized cells exhibited good operational stability and the yield of D-fructose retained above 42% within 603 h. The space–time yield of high fructose corn syrup reached 3.84 kg L−1 day−1. The investigation provided an efficient immobilization method for recombinant cells expressing glucose isomerase with higher stability, and the immobilized cells are a promising biocatalyst for HFCS production. More... »

PAGES

1781-1792

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00449-021-02560-4

DOI

http://dx.doi.org/10.1007/s00449-021-02560-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1137000359

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33830378


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