Repeated production of 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose by immobilized Gluconobacter oxydans cells with a strategy of in situ exhaustive cell regeneration View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-05-12

AUTHORS

Zhong-Ce Hu, Zi-Yu Zhao, Xia Ke, Yu-Guo Zheng

ABSTRACT

The major troubles in 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose (6NSL) production from N-2-hydroxyethyl glucamine (NHEG) by Gluconobacter oxydans were low cell yield during cell preparation and loss of cells’ biocatalytic ability during biotransformation, resulting in high production cost and low 6NSL production. The target of this work was to enhance 6NSL production by reusing cells and improving the cells biocatalytic ability. First, inhibitory effects of substrate and product on 6NSL production, and optimization of cell regeneration condition were investigated, respectively. Then repeated production of 6NSL by immobilized cell using a strategy of in situ exhaustive cell regeneration in a bubble column bioreactor was developed. As a result, the bioprocess underwent nine cycles, the average 6NSL production and conversion rate of NHEG to 6NSL reached 42.6 g L−1 and 83.1% in each batch was achieved, respectively. More... »

PAGES

1781-1789

References to SciGraph publications

  • 2000-03. Miglitol in DRUGS
  • 2018-06-15. Improving techno-economics of bioproduct glycolic acid by successive recycled-cell catalysis of ethylene glycol with Gluconobacter oxydans in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • 2018-05-02. Batch and Repeated-Batch Fermentation for 1,3-Dihydroxyacetone Production from Waste Glycerol Using Free, Immobilized and Resting Gluconobacter oxydans Cells in WASTE AND BIOMASS VALORIZATION
  • 1993-02. Inhibitory effect of dihydroxyacetone onGluconobacter oxydans: Kinetic aspects and expression by mathematical equations in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2017-05-08. Metabolic engineering of Gluconobacter oxydans 621H for increased biomass yield in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2018-11-15. Enhanced Production of 6-(N-Hydroxyethyl)-Amino-6-Deoxy-α-L-Sorbofuranose by Immobilized Gluconobacter oxydanson Corn Stover with a pH Control Strategy in a Bubble Column Bioreactor in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2005-01-23. Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans in NATURE BIOTECHNOLOGY
  • 2018-04-28. Biosynthesis of miglitol intermediate 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose by an improved d-sorbitol dehydrogenase from Gluconobacter oxydans in 3 BIOTECH
  • 2016-06-15. Central Carbon Metabolism and Respiration in Gluconobacter oxydans in ACETIC ACID BACTERIA
  • 2010-03-06. Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00449-020-02368-8

    DOI

    http://dx.doi.org/10.1007/s00449-020-02368-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1127539794

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32399751


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Technology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0903", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biomedical Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0904", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1003", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Industrial Biotechnology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bioreactors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Immobilized", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gluconobacter oxydans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sorbose", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hu", 
            "givenName": "Zhong-Ce", 
            "id": "sg:person.01364377573.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364377573.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhao", 
            "givenName": "Zi-Yu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ke", 
            "givenName": "Xia", 
            "id": "sg:person.07670216753.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07670216753.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zheng", 
            "givenName": "Yu-Guo", 
            "id": "sg:person.01144225405.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.2165/00003495-200059030-00012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044142052", 
              "https://doi.org/10.2165/00003495-200059030-00012"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s13205-018-1251-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103676322", 
              "https://doi.org/10.1007/s13205-018-1251-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01583682", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027078710", 
              "https://doi.org/10.1007/bf01583682"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1062", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037536165", 
              "https://doi.org/10.1038/nbt1062"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-017-8308-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085210842", 
              "https://doi.org/10.1007/s00253-017-8308-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12649-018-0307-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103762947", 
              "https://doi.org/10.1007/s12649-018-0307-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12010-018-2924-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1109907371", 
              "https://doi.org/10.1007/s12010-018-2924-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10295-010-0703-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049728064", 
              "https://doi.org/10.1007/s10295-010-0703-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-4-431-55933-7_11", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048238608", 
              "https://doi.org/10.1007/978-4-431-55933-7_11"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00449-018-1968-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1104605335", 
              "https://doi.org/10.1007/s00449-018-1968-2"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2020-05-12", 
        "datePublishedReg": "2020-05-12", 
        "description": "The major troubles in 6-(N-hydroxyethyl)-amino-6-deoxy-\u03b1-l-sorbofuranose (6NSL) production from N-2-hydroxyethyl glucamine (NHEG) by Gluconobacter oxydans were low cell yield during cell preparation and loss of cells\u2019 biocatalytic ability during biotransformation, resulting in high production cost and low 6NSL production. The target of this work was to enhance 6NSL production by reusing cells and improving the cells biocatalytic ability. First, inhibitory effects of substrate and product on 6NSL production, and optimization of cell regeneration condition were investigated, respectively. Then repeated production of 6NSL by immobilized cell using a strategy of in situ exhaustive cell regeneration in a bubble column bioreactor was developed. As a result, the bioprocess underwent nine cycles, the average 6NSL production and conversion rate of NHEG to 6NSL reached 42.6\u00a0g\u00a0L\u22121 and 83.1% in each batch was achieved, respectively.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s00449-020-02368-8", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1297453", 
            "issn": [
              "0178-515X", 
              "1432-0797"
            ], 
            "name": "Bioprocess and Biosystems Engineering", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "10", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "43"
          }
        ], 
        "keywords": [
          "biocatalytic ability", 
          "Gluconobacter oxydans", 
          "high production costs", 
          "lower cell yield", 
          "bubble column bioreactor", 
          "column bioreactor", 
          "regeneration conditions", 
          "oxydans", 
          "production costs", 
          "NHEG", 
          "cell yield", 
          "bioprocesses", 
          "sorbofuranose", 
          "bioreactor", 
          "regeneration", 
          "cell regeneration", 
          "substrate", 
          "conversion rate", 
          "major trouble", 
          "production", 
          "preparation", 
          "cells", 
          "strategies", 
          "cell preparations", 
          "yield", 
          "ability", 
          "cost", 
          "optimization", 
          "batch", 
          "biotransformation", 
          "work", 
          "loss of cells", 
          "products", 
          "loss", 
          "cycle", 
          "rate", 
          "results", 
          "conditions", 
          "glucamine", 
          "target", 
          "effect", 
          "inhibitory effect", 
          "trouble", 
          "sorbofuranose (6NSL) production", 
          "cells biocatalytic ability", 
          "cell regeneration condition", 
          "exhaustive cell regeneration"
        ], 
        "name": "Repeated production of 6-(N-hydroxyethyl)-amino-6-deoxy-\u03b1-l-sorbofuranose by immobilized Gluconobacter oxydans cells with a strategy of in situ exhaustive cell regeneration", 
        "pagination": "1781-1789", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1127539794"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00449-020-02368-8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "32399751"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00449-020-02368-8", 
          "https://app.dimensions.ai/details/publication/pub.1127539794"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-01-01T18:54", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_837.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s00449-020-02368-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02368-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02368-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02368-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02368-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    197 TRIPLES      22 PREDICATES      90 URIs      69 LITERALS      11 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00449-020-02368-8 schema:about N232f5128c5894c3eab5f272b7b033bf4
    2 N3ff7724157864ff18ca3a13270a5953f
    3 N45d57a8898d547a294498a5fc002cd3b
    4 Nb0a5304af45b433e9707b6e96aab0eae
    5 anzsrc-for:09
    6 anzsrc-for:0903
    7 anzsrc-for:0904
    8 anzsrc-for:10
    9 anzsrc-for:1003
    10 schema:author Nf9037d4d187946529f44abfcee0085cb
    11 schema:citation sg:pub.10.1007/978-4-431-55933-7_11
    12 sg:pub.10.1007/bf01583682
    13 sg:pub.10.1007/s00253-017-8308-3
    14 sg:pub.10.1007/s00449-018-1968-2
    15 sg:pub.10.1007/s10295-010-0703-z
    16 sg:pub.10.1007/s12010-018-2924-y
    17 sg:pub.10.1007/s12649-018-0307-9
    18 sg:pub.10.1007/s13205-018-1251-x
    19 sg:pub.10.1038/nbt1062
    20 sg:pub.10.2165/00003495-200059030-00012
    21 schema:datePublished 2020-05-12
    22 schema:datePublishedReg 2020-05-12
    23 schema:description The major troubles in 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose (6NSL) production from N-2-hydroxyethyl glucamine (NHEG) by Gluconobacter oxydans were low cell yield during cell preparation and loss of cells’ biocatalytic ability during biotransformation, resulting in high production cost and low 6NSL production. The target of this work was to enhance 6NSL production by reusing cells and improving the cells biocatalytic ability. First, inhibitory effects of substrate and product on 6NSL production, and optimization of cell regeneration condition were investigated, respectively. Then repeated production of 6NSL by immobilized cell using a strategy of in situ exhaustive cell regeneration in a bubble column bioreactor was developed. As a result, the bioprocess underwent nine cycles, the average 6NSL production and conversion rate of NHEG to 6NSL reached 42.6 g L−1 and 83.1% in each batch was achieved, respectively.
    24 schema:genre article
    25 schema:inLanguage en
    26 schema:isAccessibleForFree false
    27 schema:isPartOf N26cf2f0e53974414a996b1ea600dbf92
    28 Nbc501803741349f5853eabef24f7f2e0
    29 sg:journal.1297453
    30 schema:keywords Gluconobacter oxydans
    31 NHEG
    32 ability
    33 batch
    34 biocatalytic ability
    35 bioprocesses
    36 bioreactor
    37 biotransformation
    38 bubble column bioreactor
    39 cell preparations
    40 cell regeneration
    41 cell regeneration condition
    42 cell yield
    43 cells
    44 cells biocatalytic ability
    45 column bioreactor
    46 conditions
    47 conversion rate
    48 cost
    49 cycle
    50 effect
    51 exhaustive cell regeneration
    52 glucamine
    53 high production costs
    54 inhibitory effect
    55 loss
    56 loss of cells
    57 lower cell yield
    58 major trouble
    59 optimization
    60 oxydans
    61 preparation
    62 production
    63 production costs
    64 products
    65 rate
    66 regeneration
    67 regeneration conditions
    68 results
    69 sorbofuranose
    70 sorbofuranose (6NSL) production
    71 strategies
    72 substrate
    73 target
    74 trouble
    75 work
    76 yield
    77 schema:name Repeated production of 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose by immobilized Gluconobacter oxydans cells with a strategy of in situ exhaustive cell regeneration
    78 schema:pagination 1781-1789
    79 schema:productId N1725d42fe714488e8072566150f4fe58
    80 N70d5a8c752c14d5aab0460f5061a4b34
    81 Nf1e71ebbe1d7489f85f2b5ba551e5773
    82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1127539794
    83 https://doi.org/10.1007/s00449-020-02368-8
    84 schema:sdDatePublished 2022-01-01T18:54
    85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    86 schema:sdPublisher Ndb6b22d484f94e38b2adb165acb5380e
    87 schema:url https://doi.org/10.1007/s00449-020-02368-8
    88 sgo:license sg:explorer/license/
    89 sgo:sdDataset articles
    90 rdf:type schema:ScholarlyArticle
    91 N1725d42fe714488e8072566150f4fe58 schema:name dimensions_id
    92 schema:value pub.1127539794
    93 rdf:type schema:PropertyValue
    94 N232f5128c5894c3eab5f272b7b033bf4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Cells, Immobilized
    96 rdf:type schema:DefinedTerm
    97 N26cf2f0e53974414a996b1ea600dbf92 schema:volumeNumber 43
    98 rdf:type schema:PublicationVolume
    99 N3ff7724157864ff18ca3a13270a5953f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Sorbose
    101 rdf:type schema:DefinedTerm
    102 N45d57a8898d547a294498a5fc002cd3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Gluconobacter oxydans
    104 rdf:type schema:DefinedTerm
    105 N626f70bb24274ed9a372f172362bad70 schema:affiliation grid-institutes:grid.469325.f
    106 schema:familyName Zhao
    107 schema:givenName Zi-Yu
    108 rdf:type schema:Person
    109 N70d5a8c752c14d5aab0460f5061a4b34 schema:name pubmed_id
    110 schema:value 32399751
    111 rdf:type schema:PropertyValue
    112 N9ca70c14acac4ecdb9298af2caaf35e9 rdf:first sg:person.01144225405.06
    113 rdf:rest rdf:nil
    114 Naefe493b543d4f0c820c65bc02f73529 rdf:first sg:person.07670216753.29
    115 rdf:rest N9ca70c14acac4ecdb9298af2caaf35e9
    116 Nb0a5304af45b433e9707b6e96aab0eae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Bioreactors
    118 rdf:type schema:DefinedTerm
    119 Nbc501803741349f5853eabef24f7f2e0 schema:issueNumber 10
    120 rdf:type schema:PublicationIssue
    121 Ndb6b22d484f94e38b2adb165acb5380e schema:name Springer Nature - SN SciGraph project
    122 rdf:type schema:Organization
    123 Nedd0e94fe75f4c2ca95b43dfe93bc16e rdf:first N626f70bb24274ed9a372f172362bad70
    124 rdf:rest Naefe493b543d4f0c820c65bc02f73529
    125 Nf1e71ebbe1d7489f85f2b5ba551e5773 schema:name doi
    126 schema:value 10.1007/s00449-020-02368-8
    127 rdf:type schema:PropertyValue
    128 Nf9037d4d187946529f44abfcee0085cb rdf:first sg:person.01364377573.36
    129 rdf:rest Nedd0e94fe75f4c2ca95b43dfe93bc16e
    130 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
    131 schema:name Engineering
    132 rdf:type schema:DefinedTerm
    133 anzsrc-for:0903 schema:inDefinedTermSet anzsrc-for:
    134 schema:name Biomedical Engineering
    135 rdf:type schema:DefinedTerm
    136 anzsrc-for:0904 schema:inDefinedTermSet anzsrc-for:
    137 schema:name Chemical Engineering
    138 rdf:type schema:DefinedTerm
    139 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
    140 schema:name Technology
    141 rdf:type schema:DefinedTerm
    142 anzsrc-for:1003 schema:inDefinedTermSet anzsrc-for:
    143 schema:name Industrial Biotechnology
    144 rdf:type schema:DefinedTerm
    145 sg:journal.1297453 schema:issn 0178-515X
    146 1432-0797
    147 schema:name Bioprocess and Biosystems Engineering
    148 schema:publisher Springer Nature
    149 rdf:type schema:Periodical
    150 sg:person.01144225405.06 schema:affiliation grid-institutes:grid.469325.f
    151 schema:familyName Zheng
    152 schema:givenName Yu-Guo
    153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
    154 rdf:type schema:Person
    155 sg:person.01364377573.36 schema:affiliation grid-institutes:grid.469325.f
    156 schema:familyName Hu
    157 schema:givenName Zhong-Ce
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364377573.36
    159 rdf:type schema:Person
    160 sg:person.07670216753.29 schema:affiliation grid-institutes:grid.469325.f
    161 schema:familyName Ke
    162 schema:givenName Xia
    163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07670216753.29
    164 rdf:type schema:Person
    165 sg:pub.10.1007/978-4-431-55933-7_11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048238608
    166 https://doi.org/10.1007/978-4-431-55933-7_11
    167 rdf:type schema:CreativeWork
    168 sg:pub.10.1007/bf01583682 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027078710
    169 https://doi.org/10.1007/bf01583682
    170 rdf:type schema:CreativeWork
    171 sg:pub.10.1007/s00253-017-8308-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085210842
    172 https://doi.org/10.1007/s00253-017-8308-3
    173 rdf:type schema:CreativeWork
    174 sg:pub.10.1007/s00449-018-1968-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104605335
    175 https://doi.org/10.1007/s00449-018-1968-2
    176 rdf:type schema:CreativeWork
    177 sg:pub.10.1007/s10295-010-0703-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1049728064
    178 https://doi.org/10.1007/s10295-010-0703-z
    179 rdf:type schema:CreativeWork
    180 sg:pub.10.1007/s12010-018-2924-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1109907371
    181 https://doi.org/10.1007/s12010-018-2924-y
    182 rdf:type schema:CreativeWork
    183 sg:pub.10.1007/s12649-018-0307-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103762947
    184 https://doi.org/10.1007/s12649-018-0307-9
    185 rdf:type schema:CreativeWork
    186 sg:pub.10.1007/s13205-018-1251-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1103676322
    187 https://doi.org/10.1007/s13205-018-1251-x
    188 rdf:type schema:CreativeWork
    189 sg:pub.10.1038/nbt1062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037536165
    190 https://doi.org/10.1038/nbt1062
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.2165/00003495-200059030-00012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044142052
    193 https://doi.org/10.2165/00003495-200059030-00012
    194 rdf:type schema:CreativeWork
    195 grid-institutes:grid.469325.f schema:alternateName Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
    196 schema:name Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
    197 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...