Covalent immobilization of recombinant Citrobacter koseri transaminase onto epoxy resins for consecutive asymmetric synthesis of L-phosphinothricin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-04-24

AUTHORS

Dong-Xu Jia, Hai-Peng Xu, Chen-Yi Sun, Chen Peng, Jun-Liang Li, Li-Qun Jin, Feng Cheng, Zhi-Qiang Liu, Ya-Ping Xue, Yu-Guo Zheng

ABSTRACT

Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. Using optimum ES-105 support, a higher immobilized activity was obtained via optimizing immobilization process in terms of enzyme loading, coupling time and initial PLP concentration. Crucially, due to blocking unreacted epoxy groups on support surface with amino acids, the reaction temperature of blocked immobilized biocatalyst was enhanced from 37 to 57 °C. Its thermostability at 57 °C was also found to be superior to that of free CkTA. The Km value was shifted from 36.75 mM of free CkTA to 39.87 mM of blocked immobilized biocatalyst, demonstrating that the affinity of enzyme to the substrate has not been apparently altered. Accordingly, the biocatalyst performed the consecutive synthesis of L-PPT for 11 cycles (yields>91%) with retaining more than 91.13% of the initial activity. The seemingly the highest reusability demonstrates this biocatalyst has prospective for reducing the costs of consecutive synthesis of L-PPT with high conversion. More... »

PAGES

1599-1607

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00449-020-02351-3

DOI

http://dx.doi.org/10.1007/s00449-020-02351-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1127128594

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/32333194


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0903", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biomedical Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0904", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1003", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Industrial Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aminobutyrates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Citrobacter koseri", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzymes, Immobilized", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epoxy Resins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transaminases", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jia", 
        "givenName": "Dong-Xu", 
        "id": "sg:person.01335722620.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335722620.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Hai-Peng", 
        "id": "sg:person.013324156723.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013324156723.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sun", 
        "givenName": "Chen-Yi", 
        "id": "sg:person.016124217371.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016124217371.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peng", 
        "givenName": "Chen", 
        "id": "sg:person.07421146171.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07421146171.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Jun-Liang", 
        "id": "sg:person.014121537323.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014121537323.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jin", 
        "givenName": "Li-Qun", 
        "id": "sg:person.01105203476.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105203476.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheng", 
        "givenName": "Feng", 
        "id": "sg:person.0607172370.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607172370.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Zhi-Qiang", 
        "id": "sg:person.01256734541.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xue", 
        "givenName": "Ya-Ping", 
        "id": "sg:person.0744724672.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China", 
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Yu-Guo", 
        "id": "sg:person.01144225405.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00253-007-1059-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023202657", 
          "https://doi.org/10.1007/s00253-007-1059-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-008-0426-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015215091", 
          "https://doi.org/10.1007/s10295-008-0426-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12010-013-0498-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018708485", 
          "https://doi.org/10.1007/s12010-013-0498-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00394580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001176216", 
          "https://doi.org/10.1007/bf00394580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-018-9310-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1106525980", 
          "https://doi.org/10.1007/s00253-018-9310-0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2020-04-24", 
    "datePublishedReg": "2020-04-24", 
    "description": "Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. Using optimum ES-105 support, a higher immobilized activity was obtained via optimizing immobilization process in terms of enzyme loading, coupling time and initial PLP concentration. Crucially, due to blocking unreacted epoxy groups on support surface with amino acids, the reaction temperature of blocked immobilized biocatalyst was enhanced from 37 to 57\u00a0\u00b0C. Its thermostability at 57\u00a0\u00b0C was also found to be superior to that of free CkTA. The Km value was shifted from 36.75\u00a0mM of free CkTA to 39.87\u00a0mM of blocked immobilized biocatalyst, demonstrating that the affinity of enzyme to the substrate has not been apparently altered. Accordingly, the biocatalyst performed the consecutive synthesis of L-PPT for 11 cycles (yields>91%) with retaining more than 91.13% of the initial activity. The seemingly the highest reusability demonstrates this biocatalyst has prospective for reducing the costs of consecutive synthesis of L-PPT with high conversion.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00449-020-02351-3", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.8906786", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.8198962", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1297453", 
        "issn": [
          "0178-515X", 
          "1432-0797"
        ], 
        "name": "Bioprocess and Biosystems Engineering", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "43"
      }
    ], 
    "keywords": [
      "covalent immobilization", 
      "consecutive synthesis", 
      "immobilized biocatalyst", 
      "recombinant transaminase", 
      "enzyme loading", 
      "high reusability", 
      "biocatalyst", 
      "epoxy resin", 
      "immobilization process", 
      "reaction temperature", 
      "initial activity", 
      "herbicide L", 
      "epoxy groups", 
      "phosphinothricin", 
      "immobilization", 
      "unreacted epoxy groups", 
      "synthesis", 
      "different epoxy resins", 
      "support surface", 
      "high conversion", 
      "reusability", 
      "affinity of enzyme", 
      "substrate", 
      "thermostability", 
      "surface", 
      "amino acids", 
      "resin", 
      "conversion", 
      "Km values", 
      "loading", 
      "cost", 
      "mM", 
      "temperature", 
      "enzyme", 
      "work", 
      "acid", 
      "activity", 
      "concentration", 
      "affinity", 
      "process", 
      "cycle", 
      "ppt", 
      "time", 
      "terms", 
      "ketone compounds", 
      "Citrobacter", 
      "support", 
      "compounds", 
      "values", 
      "asymmetric synthesis", 
      "transaminase", 
      "group", 
      "PLP concentrations", 
      "immobilized activity", 
      "prochiral ketone compound", 
      "optimum ES-105 support", 
      "ES-105 support", 
      "higher immobilized activity", 
      "initial PLP concentration", 
      "free CkTA", 
      "CkTA", 
      "recombinant Citrobacter", 
      "consecutive asymmetric synthesis"
    ], 
    "name": "Covalent immobilization of recombinant Citrobacter koseri transaminase onto epoxy resins for consecutive asymmetric synthesis of L-phosphinothricin", 
    "pagination": "1599-1607", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1127128594"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00449-020-02351-3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "32333194"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00449-020-02351-3", 
      "https://app.dimensions.ai/details/publication/pub.1127128594"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T19:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_870.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00449-020-02351-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02351-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02351-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02351-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00449-020-02351-3'


 

This table displays all metadata directly associated to this object as RDF triples.

253 TRIPLES      22 PREDICATES      104 URIs      88 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00449-020-02351-3 schema:about N19f1d2298b9045099a278ac0e89df585
2 N26d5731ff6d24b359cb296c9408c6b91
3 N285dd5618370470baa6e1b3c0e0293fc
4 N55082cb10c3149f0ba4a3f44692e35a4
5 N5c7140776e06442d8e4efed48d19757b
6 Naa71a72ce63b429f9c5a1764048bc586
7 Ne6cc543d40964e57ae11860d8cfb0528
8 anzsrc-for:09
9 anzsrc-for:0903
10 anzsrc-for:0904
11 anzsrc-for:10
12 anzsrc-for:1003
13 schema:author N2f85076bd55d4c3582b8a1705b0e148f
14 schema:citation sg:pub.10.1007/bf00394580
15 sg:pub.10.1007/s00253-007-1059-9
16 sg:pub.10.1007/s00253-018-9310-0
17 sg:pub.10.1007/s10295-008-0426-6
18 sg:pub.10.1007/s12010-013-0498-2
19 schema:datePublished 2020-04-24
20 schema:datePublishedReg 2020-04-24
21 schema:description Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. Using optimum ES-105 support, a higher immobilized activity was obtained via optimizing immobilization process in terms of enzyme loading, coupling time and initial PLP concentration. Crucially, due to blocking unreacted epoxy groups on support surface with amino acids, the reaction temperature of blocked immobilized biocatalyst was enhanced from 37 to 57 °C. Its thermostability at 57 °C was also found to be superior to that of free CkTA. The Km value was shifted from 36.75 mM of free CkTA to 39.87 mM of blocked immobilized biocatalyst, demonstrating that the affinity of enzyme to the substrate has not been apparently altered. Accordingly, the biocatalyst performed the consecutive synthesis of L-PPT for 11 cycles (yields>91%) with retaining more than 91.13% of the initial activity. The seemingly the highest reusability demonstrates this biocatalyst has prospective for reducing the costs of consecutive synthesis of L-PPT with high conversion.
22 schema:genre article
23 schema:inLanguage en
24 schema:isAccessibleForFree false
25 schema:isPartOf Ne734c248f9ce4824b41bb2202163e5aa
26 Nf6ec47a96afb4687923eac745759d421
27 sg:journal.1297453
28 schema:keywords Citrobacter
29 CkTA
30 ES-105 support
31 Km values
32 PLP concentrations
33 acid
34 activity
35 affinity
36 affinity of enzyme
37 amino acids
38 asymmetric synthesis
39 biocatalyst
40 compounds
41 concentration
42 consecutive asymmetric synthesis
43 consecutive synthesis
44 conversion
45 cost
46 covalent immobilization
47 cycle
48 different epoxy resins
49 enzyme
50 enzyme loading
51 epoxy groups
52 epoxy resin
53 free CkTA
54 group
55 herbicide L
56 high conversion
57 high reusability
58 higher immobilized activity
59 immobilization
60 immobilization process
61 immobilized activity
62 immobilized biocatalyst
63 initial PLP concentration
64 initial activity
65 ketone compounds
66 loading
67 mM
68 optimum ES-105 support
69 phosphinothricin
70 ppt
71 process
72 prochiral ketone compound
73 reaction temperature
74 recombinant Citrobacter
75 recombinant transaminase
76 resin
77 reusability
78 substrate
79 support
80 support surface
81 surface
82 synthesis
83 temperature
84 terms
85 thermostability
86 time
87 transaminase
88 unreacted epoxy groups
89 values
90 work
91 schema:name Covalent immobilization of recombinant Citrobacter koseri transaminase onto epoxy resins for consecutive asymmetric synthesis of L-phosphinothricin
92 schema:pagination 1599-1607
93 schema:productId N4929f73c14b142d39e98111696a2b3d4
94 Naea930990e3e49d69a54be3afb97c633
95 Nc1af2ad9e96b4cbfbd0245ea21597535
96 schema:sameAs https://app.dimensions.ai/details/publication/pub.1127128594
97 https://doi.org/10.1007/s00449-020-02351-3
98 schema:sdDatePublished 2022-01-01T19:00
99 schema:sdLicense https://scigraph.springernature.com/explorer/license/
100 schema:sdPublisher N3e7f5f8a5d9043519a285d96e4908b75
101 schema:url https://doi.org/10.1007/s00449-020-02351-3
102 sgo:license sg:explorer/license/
103 sgo:sdDataset articles
104 rdf:type schema:ScholarlyArticle
105 N19f1d2298b9045099a278ac0e89df585 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Bacterial Proteins
107 rdf:type schema:DefinedTerm
108 N26d5731ff6d24b359cb296c9408c6b91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Aminobutyrates
110 rdf:type schema:DefinedTerm
111 N285dd5618370470baa6e1b3c0e0293fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Recombinant Proteins
113 rdf:type schema:DefinedTerm
114 N2e2ead51db974589868e02049883d857 rdf:first sg:person.01105203476.08
115 rdf:rest Nb5b35e901d6b4247af69c495ab1c100d
116 N2f85076bd55d4c3582b8a1705b0e148f rdf:first sg:person.01335722620.01
117 rdf:rest Nf2a7c2d5cc644b25bdd4d7bf09c93742
118 N3e7f5f8a5d9043519a285d96e4908b75 schema:name Springer Nature - SN SciGraph project
119 rdf:type schema:Organization
120 N4929f73c14b142d39e98111696a2b3d4 schema:name doi
121 schema:value 10.1007/s00449-020-02351-3
122 rdf:type schema:PropertyValue
123 N55082cb10c3149f0ba4a3f44692e35a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Transaminases
125 rdf:type schema:DefinedTerm
126 N5c7140776e06442d8e4efed48d19757b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Epoxy Resins
128 rdf:type schema:DefinedTerm
129 N5d2f82d4733e40cc8cd947183a147519 rdf:first sg:person.01144225405.06
130 rdf:rest rdf:nil
131 N728ba2884f0e4494aca9a4dd48a99f5f rdf:first sg:person.014121537323.06
132 rdf:rest N2e2ead51db974589868e02049883d857
133 N83132ee195204ffbb49b0a03cd959081 rdf:first sg:person.016124217371.75
134 rdf:rest Na717c555cb634ed3ac7a4e1640f8d216
135 Na717c555cb634ed3ac7a4e1640f8d216 rdf:first sg:person.07421146171.90
136 rdf:rest N728ba2884f0e4494aca9a4dd48a99f5f
137 Naa71a72ce63b429f9c5a1764048bc586 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Citrobacter koseri
139 rdf:type schema:DefinedTerm
140 Naea930990e3e49d69a54be3afb97c633 schema:name dimensions_id
141 schema:value pub.1127128594
142 rdf:type schema:PropertyValue
143 Nb5b35e901d6b4247af69c495ab1c100d rdf:first sg:person.0607172370.20
144 rdf:rest Ncbf3ee1eda504e72a8a214c5098e5139
145 Nc006ab3977ce483db29ae162ba5592aa rdf:first sg:person.0744724672.04
146 rdf:rest N5d2f82d4733e40cc8cd947183a147519
147 Nc1af2ad9e96b4cbfbd0245ea21597535 schema:name pubmed_id
148 schema:value 32333194
149 rdf:type schema:PropertyValue
150 Ncbf3ee1eda504e72a8a214c5098e5139 rdf:first sg:person.01256734541.85
151 rdf:rest Nc006ab3977ce483db29ae162ba5592aa
152 Ne6cc543d40964e57ae11860d8cfb0528 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Enzymes, Immobilized
154 rdf:type schema:DefinedTerm
155 Ne734c248f9ce4824b41bb2202163e5aa schema:issueNumber 9
156 rdf:type schema:PublicationIssue
157 Nf2a7c2d5cc644b25bdd4d7bf09c93742 rdf:first sg:person.013324156723.25
158 rdf:rest N83132ee195204ffbb49b0a03cd959081
159 Nf6ec47a96afb4687923eac745759d421 schema:volumeNumber 43
160 rdf:type schema:PublicationVolume
161 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
162 schema:name Engineering
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0903 schema:inDefinedTermSet anzsrc-for:
165 schema:name Biomedical Engineering
166 rdf:type schema:DefinedTerm
167 anzsrc-for:0904 schema:inDefinedTermSet anzsrc-for:
168 schema:name Chemical Engineering
169 rdf:type schema:DefinedTerm
170 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
171 schema:name Technology
172 rdf:type schema:DefinedTerm
173 anzsrc-for:1003 schema:inDefinedTermSet anzsrc-for:
174 schema:name Industrial Biotechnology
175 rdf:type schema:DefinedTerm
176 sg:grant.8198962 http://pending.schema.org/fundedItem sg:pub.10.1007/s00449-020-02351-3
177 rdf:type schema:MonetaryGrant
178 sg:grant.8906786 http://pending.schema.org/fundedItem sg:pub.10.1007/s00449-020-02351-3
179 rdf:type schema:MonetaryGrant
180 sg:journal.1297453 schema:issn 0178-515X
181 1432-0797
182 schema:name Bioprocess and Biosystems Engineering
183 schema:publisher Springer Nature
184 rdf:type schema:Periodical
185 sg:person.01105203476.08 schema:affiliation grid-institutes:grid.469325.f
186 schema:familyName Jin
187 schema:givenName Li-Qun
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105203476.08
189 rdf:type schema:Person
190 sg:person.01144225405.06 schema:affiliation grid-institutes:grid.469325.f
191 schema:familyName Zheng
192 schema:givenName Yu-Guo
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
194 rdf:type schema:Person
195 sg:person.01256734541.85 schema:affiliation grid-institutes:grid.469325.f
196 schema:familyName Liu
197 schema:givenName Zhi-Qiang
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85
199 rdf:type schema:Person
200 sg:person.013324156723.25 schema:affiliation grid-institutes:grid.469325.f
201 schema:familyName Xu
202 schema:givenName Hai-Peng
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013324156723.25
204 rdf:type schema:Person
205 sg:person.01335722620.01 schema:affiliation grid-institutes:grid.469325.f
206 schema:familyName Jia
207 schema:givenName Dong-Xu
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335722620.01
209 rdf:type schema:Person
210 sg:person.014121537323.06 schema:affiliation grid-institutes:grid.469325.f
211 schema:familyName Li
212 schema:givenName Jun-Liang
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014121537323.06
214 rdf:type schema:Person
215 sg:person.016124217371.75 schema:affiliation grid-institutes:grid.469325.f
216 schema:familyName Sun
217 schema:givenName Chen-Yi
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016124217371.75
219 rdf:type schema:Person
220 sg:person.0607172370.20 schema:affiliation grid-institutes:grid.469325.f
221 schema:familyName Cheng
222 schema:givenName Feng
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607172370.20
224 rdf:type schema:Person
225 sg:person.07421146171.90 schema:affiliation grid-institutes:grid.469325.f
226 schema:familyName Peng
227 schema:givenName Chen
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07421146171.90
229 rdf:type schema:Person
230 sg:person.0744724672.04 schema:affiliation grid-institutes:grid.469325.f
231 schema:familyName Xue
232 schema:givenName Ya-Ping
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04
234 rdf:type schema:Person
235 sg:pub.10.1007/bf00394580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001176216
236 https://doi.org/10.1007/bf00394580
237 rdf:type schema:CreativeWork
238 sg:pub.10.1007/s00253-007-1059-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023202657
239 https://doi.org/10.1007/s00253-007-1059-9
240 rdf:type schema:CreativeWork
241 sg:pub.10.1007/s00253-018-9310-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106525980
242 https://doi.org/10.1007/s00253-018-9310-0
243 rdf:type schema:CreativeWork
244 sg:pub.10.1007/s10295-008-0426-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015215091
245 https://doi.org/10.1007/s10295-008-0426-6
246 rdf:type schema:CreativeWork
247 sg:pub.10.1007/s12010-013-0498-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018708485
248 https://doi.org/10.1007/s12010-013-0498-2
249 rdf:type schema:CreativeWork
250 grid-institutes:grid.469325.f schema:alternateName Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China
251 schema:name Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China
252 The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang road, 310014, Hangzhou, P. R. China
253 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...