Upscale production of (R)-mandelic acid with a stereospecific nitrilase in an aqueous system View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-03-20

AUTHORS

Xin-Hong Zhang, Chu-Yan Wang, Xue Cai, Ya-Ping Xue, Zhi-Qiang Liu, Yu-Guo Zheng

ABSTRACT

(R)-Mandelic acid (R-MA) is a key precursor for the synthesis of semi-synthetic penicillin, cephalosporin, anti-obesity drugs, antitumor agents, and chiral resolving agents for the resolution of racemic alcohols and amines. In this study, an enzymatic method for the large-scale production of R-MA by a stereospecific nitrilase in an aqueous system was developed. The nitrilase activity of the Escherichia coli BL21(DE3)/pET-Nit whole cells reached 138.6 U/g in a 20,000-L fermentor. Using recombinant E. coli cells as catalyst, 500 mM R,S-mandelonitrile (R,S-MN) was resolved into 426 mM (64.85 g/L) R-MA within 8 h, and the enantiomeric excess (ee) value of R-MA reached 99%. During the purification process, pure R-MA with a recovery rate of 78.8% was obtained after concentration and crystallization. This study paved the foundation for the upscale production of R-MA using E. coli whole cells as biocatalyst. More... »

PAGES

1299-1307

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00449-020-02326-4

DOI

http://dx.doi.org/10.1007/s00449-020-02326-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1125772108

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/32193756


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