A high-throughput screening method for improved R-2-(4-hydroxyphenoxy)propionic acid biosynthesis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-06-12

AUTHORS

Hai-Yan Zhou, Yi-Zuo Li, Rui Jiang, Hai-Feng Hu, Yuan-Shan Wang, Zhi-Qiang Liu, Ya-Ping Xue, Yu-Guo Zheng

ABSTRACT

R-2-(4-hydroxyphenoxy)propionic acid (R-HPPA) is a key intermediate of the enantiomerically pure phenoxypropionic acid herbicides. R-HPPA could be biosynthesized through selective introduction of a hydroxyl group (–OH) into the substrate R-2-phenoxypropionic acid (R-PPA) at C-4 position, facilitated by microorganisms with hydroxylases. In this study, an efficient high-throughput screening method for improved R-HPPA biosynthesis through microbial hydroxylation was developed. As a hydroxylated aromatic product, R-HPPA could be oxidized by oxidant potassium dichromate to form brown-colored quinone-type compound. The concentration of R-HPPA can be quantified according to the absorbance of the colored compound at a suitable wavelength of 570 nm; and the R-HPPA biosynthetic capability of microorganism strains could also be rapidly evaluated. After optimization of the assay conditions, the high-throughput screening method was successfully used in identification of Beauveria bassiana mutants with enhanced R-HPPA biosynthesis capacity. A positive mutant C-7 with high tolerance to 20 g/L R-PPA was rapidly selected from 1920 mutants. The biomass and R-HPPA titer were 12.5- and 38.19-fold higher compared with the original strain at 20 g/L R-PPA. This high-throughput screening method developed in this work could also be a potential tool for screening strains producing other important phenolic compounds. More... »

PAGES

1573-1582

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00449-019-02154-1

DOI

http://dx.doi.org/10.1007/s00449-019-02154-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1117065422

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31190281


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0903", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biomedical Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0904", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1003", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Industrial Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Beauveria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Testing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Propionates", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Hai-Yan", 
        "id": "sg:person.01274560471.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274560471.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Yi-Zuo", 
        "id": "sg:person.014132071673.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014132071673.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jiang", 
        "givenName": "Rui", 
        "id": "sg:person.016322413273.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016322413273.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Hai-Feng", 
        "id": "sg:person.01224774661.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224774661.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Yuan-Shan", 
        "id": "sg:person.0613144457.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613144457.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Zhi-Qiang", 
        "id": "sg:person.01256734541.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xue", 
        "givenName": "Ya-Ping", 
        "id": "sg:person.0744724672.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
          "id": "http://www.grid.ac/institutes/grid.469325.f", 
          "name": [
            "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People\u2019s Republic of China", 
            "Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Yu-Guo", 
        "id": "sg:person.01144225405.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00253-008-1704-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027591760", 
          "https://doi.org/10.1007/s00253-008-1704-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-007-6362-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047317368", 
          "https://doi.org/10.1007/s00018-007-6362-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00775-010-0671-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017675311", 
          "https://doi.org/10.1007/s00775-010-0671-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00449-012-0741-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020582693", 
          "https://doi.org/10.1007/s00449-012-0741-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-016-7381-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050585465", 
          "https://doi.org/10.1007/s00253-016-7381-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-012-5799-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024802925", 
          "https://doi.org/10.1007/s00216-012-5799-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00449-018-1993-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105912968", 
          "https://doi.org/10.1007/s00449-018-1993-1"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-06-12", 
    "datePublishedReg": "2019-06-12", 
    "description": "R-2-(4-hydroxyphenoxy)propionic acid (R-HPPA) is a key intermediate of the enantiomerically pure phenoxypropionic acid herbicides. R-HPPA could be biosynthesized through selective introduction of a hydroxyl group (\u2013OH) into the substrate R-2-phenoxypropionic acid (R-PPA) at C-4 position, facilitated by microorganisms with hydroxylases. In this study, an efficient high-throughput screening method for improved R-HPPA biosynthesis through microbial hydroxylation was developed. As a hydroxylated aromatic product, R-HPPA could be oxidized by oxidant potassium dichromate to form brown-colored quinone-type compound. The concentration of R-HPPA can be quantified according to the absorbance of the colored compound at a suitable wavelength of 570\u00a0nm; and the R-HPPA biosynthetic capability of microorganism strains could also be rapidly evaluated. After optimization of the assay conditions, the high-throughput screening method was successfully used in identification of Beauveria bassiana mutants with enhanced R-HPPA biosynthesis capacity. A positive mutant C-7 with high tolerance to 20\u00a0g/L R-PPA was rapidly selected from 1920 mutants. The biomass and R-HPPA titer were 12.5- and 38.19-fold higher compared with the original strain at 20\u00a0g/L R-PPA. This high-throughput screening method developed in this work could also be a potential tool for screening strains producing other important phenolic compounds.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00449-019-02154-1", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.8186142", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1297453", 
        "issn": [
          "0178-515X", 
          "1432-0797"
        ], 
        "name": "Bioprocess and Biosystems Engineering", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "42"
      }
    ], 
    "keywords": [
      "high-throughput screening method", 
      "efficient high-throughput screening method", 
      "acid biosynthesis", 
      "biosynthetic capabilities", 
      "biosynthesis capacity", 
      "Microbial hydroxylation", 
      "screening method", 
      "suitable wavelength", 
      "high tolerance", 
      "mutants", 
      "biosynthesis", 
      "phenoxypropionic acid herbicides", 
      "original strain", 
      "assay conditions", 
      "selective introduction", 
      "important phenolic compounds", 
      "microorganism strains", 
      "substrate R", 
      "strains", 
      "hydroxylases", 
      "HPPA", 
      "potential tool", 
      "microorganisms", 
      "acid", 
      "quinone-type compounds", 
      "wavelength", 
      "phenolic compounds", 
      "biomass", 
      "herbicides", 
      "acid herbicides", 
      "absorbance", 
      "hydroxyl groups", 
      "key intermediate", 
      "tolerance", 
      "hydroxylation", 
      "capability", 
      "identification", 
      "compounds", 
      "method", 
      "intermediates", 
      "colored compound", 
      "aromatic products", 
      "capacity", 
      "potassium dichromate", 
      "optimization", 
      "titers", 
      "work", 
      "products", 
      "concentration", 
      "introduction", 
      "tool", 
      "conditions", 
      "PPA", 
      "study", 
      "dichromate", 
      "position", 
      "group", 
      "pure phenoxypropionic acid herbicides", 
      "HPPA biosynthesis", 
      "oxidant potassium dichromate", 
      "brown-colored quinone-type compound", 
      "HPPA biosynthetic capability", 
      "Beauveria bassiana mutants", 
      "bassiana mutants", 
      "HPPA biosynthesis capacity", 
      "HPPA titer"
    ], 
    "name": "A high-throughput screening method for improved R-2-(4-hydroxyphenoxy)propionic acid biosynthesis", 
    "pagination": "1573-1582", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1117065422"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00449-019-02154-1"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "31190281"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00449-019-02154-1", 
      "https://app.dimensions.ai/details/publication/pub.1117065422"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_817.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00449-019-02154-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02154-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02154-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02154-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02154-1'


 

This table displays all metadata directly associated to this object as RDF triples.

236 TRIPLES      22 PREDICATES      106 URIs      88 LITERALS      11 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00449-019-02154-1 schema:about N048298d8da9447e0acadba379c542e01
2 N213fd125a3b8477a93ba542af7dd7a5f
3 N40befcda967b4c5499d901339484af18
4 Ndd45d173d9004875955c9e524477a652
5 anzsrc-for:09
6 anzsrc-for:0903
7 anzsrc-for:0904
8 anzsrc-for:10
9 anzsrc-for:1003
10 schema:author N002a15db66524ff8bc80aefb735e905b
11 schema:citation sg:pub.10.1007/s00018-007-6362-1
12 sg:pub.10.1007/s00216-012-5799-8
13 sg:pub.10.1007/s00253-008-1704-y
14 sg:pub.10.1007/s00253-016-7381-3
15 sg:pub.10.1007/s00449-012-0741-1
16 sg:pub.10.1007/s00449-018-1993-1
17 sg:pub.10.1007/s00775-010-0671-9
18 schema:datePublished 2019-06-12
19 schema:datePublishedReg 2019-06-12
20 schema:description R-2-(4-hydroxyphenoxy)propionic acid (R-HPPA) is a key intermediate of the enantiomerically pure phenoxypropionic acid herbicides. R-HPPA could be biosynthesized through selective introduction of a hydroxyl group (–OH) into the substrate R-2-phenoxypropionic acid (R-PPA) at C-4 position, facilitated by microorganisms with hydroxylases. In this study, an efficient high-throughput screening method for improved R-HPPA biosynthesis through microbial hydroxylation was developed. As a hydroxylated aromatic product, R-HPPA could be oxidized by oxidant potassium dichromate to form brown-colored quinone-type compound. The concentration of R-HPPA can be quantified according to the absorbance of the colored compound at a suitable wavelength of 570 nm; and the R-HPPA biosynthetic capability of microorganism strains could also be rapidly evaluated. After optimization of the assay conditions, the high-throughput screening method was successfully used in identification of Beauveria bassiana mutants with enhanced R-HPPA biosynthesis capacity. A positive mutant C-7 with high tolerance to 20 g/L R-PPA was rapidly selected from 1920 mutants. The biomass and R-HPPA titer were 12.5- and 38.19-fold higher compared with the original strain at 20 g/L R-PPA. This high-throughput screening method developed in this work could also be a potential tool for screening strains producing other important phenolic compounds.
21 schema:genre article
22 schema:inLanguage en
23 schema:isAccessibleForFree false
24 schema:isPartOf N30da3815dd42420e86df256338ae7eac
25 Ne823abd7c6e44f96be87fc8cea8577e7
26 sg:journal.1297453
27 schema:keywords Beauveria bassiana mutants
28 HPPA
29 HPPA biosynthesis
30 HPPA biosynthesis capacity
31 HPPA biosynthetic capability
32 HPPA titer
33 Microbial hydroxylation
34 PPA
35 absorbance
36 acid
37 acid biosynthesis
38 acid herbicides
39 aromatic products
40 assay conditions
41 bassiana mutants
42 biomass
43 biosynthesis
44 biosynthesis capacity
45 biosynthetic capabilities
46 brown-colored quinone-type compound
47 capability
48 capacity
49 colored compound
50 compounds
51 concentration
52 conditions
53 dichromate
54 efficient high-throughput screening method
55 group
56 herbicides
57 high tolerance
58 high-throughput screening method
59 hydroxyl groups
60 hydroxylases
61 hydroxylation
62 identification
63 important phenolic compounds
64 intermediates
65 introduction
66 key intermediate
67 method
68 microorganism strains
69 microorganisms
70 mutants
71 optimization
72 original strain
73 oxidant potassium dichromate
74 phenolic compounds
75 phenoxypropionic acid herbicides
76 position
77 potassium dichromate
78 potential tool
79 products
80 pure phenoxypropionic acid herbicides
81 quinone-type compounds
82 screening method
83 selective introduction
84 strains
85 study
86 substrate R
87 suitable wavelength
88 titers
89 tolerance
90 tool
91 wavelength
92 work
93 schema:name A high-throughput screening method for improved R-2-(4-hydroxyphenoxy)propionic acid biosynthesis
94 schema:pagination 1573-1582
95 schema:productId N4655bc0347044bc9b18b8201cb7acc66
96 N4f26a2caad8c4c27904d0c240c310715
97 Naec4edd2004246f2b995841bfd68395a
98 schema:sameAs https://app.dimensions.ai/details/publication/pub.1117065422
99 https://doi.org/10.1007/s00449-019-02154-1
100 schema:sdDatePublished 2022-01-01T18:52
101 schema:sdLicense https://scigraph.springernature.com/explorer/license/
102 schema:sdPublisher Ned596eb79a394712a9b54ff089ce1844
103 schema:url https://doi.org/10.1007/s00449-019-02154-1
104 sgo:license sg:explorer/license/
105 sgo:sdDataset articles
106 rdf:type schema:ScholarlyArticle
107 N002a15db66524ff8bc80aefb735e905b rdf:first sg:person.01274560471.40
108 rdf:rest N8c16bea8b8bd40a2985d57aa15c84175
109 N048298d8da9447e0acadba379c542e01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Genetic Testing
111 rdf:type schema:DefinedTerm
112 N1887217de3144823b7162b574610b984 rdf:first sg:person.0744724672.04
113 rdf:rest N245601745a5a46d7a975b0387dc78c58
114 N213fd125a3b8477a93ba542af7dd7a5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Propionates
116 rdf:type schema:DefinedTerm
117 N245601745a5a46d7a975b0387dc78c58 rdf:first sg:person.01144225405.06
118 rdf:rest rdf:nil
119 N264c23b544c84a039a87d72a056741d4 rdf:first sg:person.01224774661.12
120 rdf:rest Nc82184c1f02242ad8a9db959022dc043
121 N30da3815dd42420e86df256338ae7eac schema:issueNumber 10
122 rdf:type schema:PublicationIssue
123 N40befcda967b4c5499d901339484af18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Mutation
125 rdf:type schema:DefinedTerm
126 N4227684e4aca4ea682841f11a3825cd6 rdf:first sg:person.01256734541.85
127 rdf:rest N1887217de3144823b7162b574610b984
128 N4655bc0347044bc9b18b8201cb7acc66 schema:name doi
129 schema:value 10.1007/s00449-019-02154-1
130 rdf:type schema:PropertyValue
131 N4f26a2caad8c4c27904d0c240c310715 schema:name dimensions_id
132 schema:value pub.1117065422
133 rdf:type schema:PropertyValue
134 N6809f6bfc26e4b8482cc57c8b52a009c rdf:first sg:person.016322413273.78
135 rdf:rest N264c23b544c84a039a87d72a056741d4
136 N8c16bea8b8bd40a2985d57aa15c84175 rdf:first sg:person.014132071673.75
137 rdf:rest N6809f6bfc26e4b8482cc57c8b52a009c
138 Naec4edd2004246f2b995841bfd68395a schema:name pubmed_id
139 schema:value 31190281
140 rdf:type schema:PropertyValue
141 Nc82184c1f02242ad8a9db959022dc043 rdf:first sg:person.0613144457.79
142 rdf:rest N4227684e4aca4ea682841f11a3825cd6
143 Ndd45d173d9004875955c9e524477a652 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Beauveria
145 rdf:type schema:DefinedTerm
146 Ne823abd7c6e44f96be87fc8cea8577e7 schema:volumeNumber 42
147 rdf:type schema:PublicationVolume
148 Ned596eb79a394712a9b54ff089ce1844 schema:name Springer Nature - SN SciGraph project
149 rdf:type schema:Organization
150 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
151 schema:name Engineering
152 rdf:type schema:DefinedTerm
153 anzsrc-for:0903 schema:inDefinedTermSet anzsrc-for:
154 schema:name Biomedical Engineering
155 rdf:type schema:DefinedTerm
156 anzsrc-for:0904 schema:inDefinedTermSet anzsrc-for:
157 schema:name Chemical Engineering
158 rdf:type schema:DefinedTerm
159 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
160 schema:name Technology
161 rdf:type schema:DefinedTerm
162 anzsrc-for:1003 schema:inDefinedTermSet anzsrc-for:
163 schema:name Industrial Biotechnology
164 rdf:type schema:DefinedTerm
165 sg:grant.8186142 http://pending.schema.org/fundedItem sg:pub.10.1007/s00449-019-02154-1
166 rdf:type schema:MonetaryGrant
167 sg:journal.1297453 schema:issn 0178-515X
168 1432-0797
169 schema:name Bioprocess and Biosystems Engineering
170 schema:publisher Springer Nature
171 rdf:type schema:Periodical
172 sg:person.01144225405.06 schema:affiliation grid-institutes:grid.469325.f
173 schema:familyName Zheng
174 schema:givenName Yu-Guo
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
176 rdf:type schema:Person
177 sg:person.01224774661.12 schema:affiliation grid-institutes:grid.469325.f
178 schema:familyName Hu
179 schema:givenName Hai-Feng
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224774661.12
181 rdf:type schema:Person
182 sg:person.01256734541.85 schema:affiliation grid-institutes:grid.469325.f
183 schema:familyName Liu
184 schema:givenName Zhi-Qiang
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256734541.85
186 rdf:type schema:Person
187 sg:person.01274560471.40 schema:affiliation grid-institutes:grid.469325.f
188 schema:familyName Zhou
189 schema:givenName Hai-Yan
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274560471.40
191 rdf:type schema:Person
192 sg:person.014132071673.75 schema:affiliation grid-institutes:grid.469325.f
193 schema:familyName Li
194 schema:givenName Yi-Zuo
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014132071673.75
196 rdf:type schema:Person
197 sg:person.016322413273.78 schema:affiliation grid-institutes:grid.469325.f
198 schema:familyName Jiang
199 schema:givenName Rui
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016322413273.78
201 rdf:type schema:Person
202 sg:person.0613144457.79 schema:affiliation grid-institutes:grid.469325.f
203 schema:familyName Wang
204 schema:givenName Yuan-Shan
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613144457.79
206 rdf:type schema:Person
207 sg:person.0744724672.04 schema:affiliation grid-institutes:grid.469325.f
208 schema:familyName Xue
209 schema:givenName Ya-Ping
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04
211 rdf:type schema:Person
212 sg:pub.10.1007/s00018-007-6362-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047317368
213 https://doi.org/10.1007/s00018-007-6362-1
214 rdf:type schema:CreativeWork
215 sg:pub.10.1007/s00216-012-5799-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024802925
216 https://doi.org/10.1007/s00216-012-5799-8
217 rdf:type schema:CreativeWork
218 sg:pub.10.1007/s00253-008-1704-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1027591760
219 https://doi.org/10.1007/s00253-008-1704-y
220 rdf:type schema:CreativeWork
221 sg:pub.10.1007/s00253-016-7381-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050585465
222 https://doi.org/10.1007/s00253-016-7381-3
223 rdf:type schema:CreativeWork
224 sg:pub.10.1007/s00449-012-0741-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020582693
225 https://doi.org/10.1007/s00449-012-0741-1
226 rdf:type schema:CreativeWork
227 sg:pub.10.1007/s00449-018-1993-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105912968
228 https://doi.org/10.1007/s00449-018-1993-1
229 rdf:type schema:CreativeWork
230 sg:pub.10.1007/s00775-010-0671-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017675311
231 https://doi.org/10.1007/s00775-010-0671-9
232 rdf:type schema:CreativeWork
233 grid-institutes:grid.469325.f schema:alternateName Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
234 schema:name Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
235 Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, 310014, Hangzhou, Zhejiang Province, People’s Republic of China
236 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...