Coenzyme Q production by metabolic engineered Escherichia coli strains in defined medium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03-26

AUTHORS

Irene Martínez, Patricio Zelada, Felipe Guevara, Rodrigo Andler, Viviana Urtuvia, Ivette Pacheco-Leyva, Alvaro Díaz-Barrera

ABSTRACT

Coenzyme Q (CoQ) plays an important role as an electron transporter in the respiratory chain. It is formed from a benzoquinone ring and an isoprenoid chain of a specific length depending on the organism. We constructed an engineered Escherichia coli strain (menF) unable to produce demethylmenaquinone and menaquinone, compounds that compete for both chorismate, precursor of the benzoquinone ring, and the isoprenoid chain involved in CoQ biosynthesis. In addition, a mutant strain (entC) unable to produce enterobactin, high-affinity siderophore, synthesized from chorismate, and a double mutant (entC-menF) were constructed. The use of glucose or glycerol as carbon sources was also evaluated for the production of CoQ8 in these strains. The double mutant (entC-menF) showed 18% increase in CoQ8-specific content compared to the control strain; however, the single-mutant strains did not show statistically significant differences in CoQ8-specific content respect to the control, in glucose medium in bioreactor experiments. Glycerol was significantly superior compared to glucose for the production of CoQ8 in E. coli, where the CoQ8-specific content increased 126% and 53% in the control and double-mutant strain, respectively. The expression of genes related to CoQ8 biosynthesis is reported, where the entC-menF double-mutant strain showed a significant increase in the expression of CoQ8 biosynthesis-related genes when glycerol was used as sole carbon source. The control strain did not show gene expression difference between both carbon sources, indicating a possible regulation at a different level. More... »

PAGES

1-7

References to SciGraph publications

  • 2005-04. Regulation of the Ubiquinone (Coenzyme Q) Biosynthetic Genes ubiCA in Escherichiacoli in CURRENT MICROBIOLOGY
  • 2015-06. UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis in NATURE
  • 2005-04. Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-01. Ubiquinone-10 production using Agrobacterium tumefaciens dps gene in Escherichia coli by coexpression system in MOLECULAR BIOTECHNOLOGY
  • 2017-12. Cellular factories for coenzyme Q10 production in MICROBIAL CELL FACTORIES
  • 2009-10. Coenzyme Q10 production by Rhodobacter sphaeroides in stirred tank and in airlift bioreactor in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • 2015-09. Batch production of coenzyme Q10 by recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Sphingomonasbaekryungensis in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2014-08. Improving coenzyme Q8 production in Escherichia coli employing multiple strategies in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2012. The Production of Coenzyme Q10 in Microorganisms in REPROGRAMMING MICROBIAL METABOLIC PATHWAYS
  • 2008-06. Analyzing real-time PCR data by the comparative CT method in NATURE PROTOCOLS
  • 2006-10. Amplification of 1-deoxy-d-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00449-019-02111-y

    DOI

    http://dx.doi.org/10.1007/s00449-019-02111-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1113011323

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30915537


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Pontificial Catholic University of Valparaiso", 
              "id": "https://www.grid.ac/institutes/grid.8170.e", 
              "name": [
                "Biochemical Engineering School, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Av. Brasil 2085, Casilla 4059,, Valpara\u00edso, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mart\u00ednez", 
            "givenName": "Irene", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pontificial Catholic University of Valparaiso", 
              "id": "https://www.grid.ac/institutes/grid.8170.e", 
              "name": [
                "Biochemical Engineering School, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Av. Brasil 2085, Casilla 4059,, Valpara\u00edso, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zelada", 
            "givenName": "Patricio", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pontificial Catholic University of Valparaiso", 
              "id": "https://www.grid.ac/institutes/grid.8170.e", 
              "name": [
                "Biochemical Engineering School, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Av. Brasil 2085, Casilla 4059,, Valpara\u00edso, Chile", 
                "Direcci\u00f3n de Investigaci\u00f3n y Postgrado, Universidad tecnol\u00f3gica de Chile INACAP, Av. C\u00f3ndor Norte 720, Ciudad Empresarial, Huechuraba, Santiago, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guevara", 
            "givenName": "Felipe", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Catholic University of the Maule", 
              "id": "https://www.grid.ac/institutes/grid.411964.f", 
              "name": [
                "Facultad de Ciencias Agrarias y Forestales, Universidad Cat\u00f3lica del Maule, Av. San Miguel 3605, Talca, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Andler", 
            "givenName": "Rodrigo", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pontificial Catholic University of Valparaiso", 
              "id": "https://www.grid.ac/institutes/grid.8170.e", 
              "name": [
                "Biochemical Engineering School, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Av. Brasil 2085, Casilla 4059,, Valpara\u00edso, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Urtuvia", 
            "givenName": "Viviana", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Porto", 
              "id": "https://www.grid.ac/institutes/grid.5808.5", 
              "name": [
                "Biochemical Engineering School, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Av. Brasil 2085, Casilla 4059,, Valpara\u00edso, Chile", 
                "i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto, Portugal"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pacheco-Leyva", 
            "givenName": "Ivette", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pontificial Catholic University of Valparaiso", 
              "id": "https://www.grid.ac/institutes/grid.8170.e", 
              "name": [
                "Biochemical Engineering School, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Av. Brasil 2085, Casilla 4059,, Valpara\u00edso, Chile"
              ], 
              "type": "Organization"
            }, 
            "familyName": "D\u00edaz-Barrera", 
            "givenName": "Alvaro", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/s0014-5793(97)00143-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000167444"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tibtech.2007.08.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002059525"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2728(77)90071-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002351535"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2728(77)90071-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002351535"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2009.04.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003234947"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature14559", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003482110", 
              "https://doi.org/10.1038/nature14559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0922-338x(95)91268-a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004156695"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/ba20090035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004691527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/ba20090035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004691527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2008.73", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005355981", 
              "https://doi.org/10.1038/nprot.2008.73"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/msb4100050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007788508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/msb4100050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007788508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10160", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008270581"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymben.2006.05.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008941575"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10295-015-1652-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011758248", 
              "https://doi.org/10.1007/s10295-015-1652-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00284-004-4417-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012018465", 
              "https://doi.org/10.1007/s00284-004-4417-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00284-004-4417-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012018465", 
              "https://doi.org/10.1007/s00284-004-4417-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00284-004-4417-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012018465", 
              "https://doi.org/10.1007/s00284-004-4417-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0014-5793(99)00360-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019205919"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0378-1097(03)00343-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020447832"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/biof.5520320128", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026105436"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/biof.5520320128", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026105436"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/meth.2001.1262", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027621591"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/meth.2001.1262", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027621591"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00449-008-0294-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028445126", 
              "https://doi.org/10.1007/s00449-008-0294-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00449-008-0294-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028445126", 
              "https://doi.org/10.1007/s00449-008-0294-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-006-0359-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029607486", 
              "https://doi.org/10.1007/s00253-006-0359-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-006-0359-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029607486", 
              "https://doi.org/10.1007/s00253-006-0359-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb.58.1814", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033070703"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ymben.2004.10.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033219497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1385/mb:35:1:1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033796372", 
              "https://doi.org/10.1385/mb:35:1:1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10295-014-1458-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036808906", 
              "https://doi.org/10.1007/s10295-014-1458-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.120163297", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039276805"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.pharmthera.2009.07.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043853072"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-007-5055-5_15", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048528611", 
              "https://doi.org/10.1007/978-94-007-5055-5_15"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0014-5793(97)01041-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048564892"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-004-1743-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053132398", 
              "https://doi.org/10.1007/s00253-004-1743-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/cb5008103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055226707"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077526900", 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12934-017-0646-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084251156", 
              "https://doi.org/10.1186/s12934-017-0646-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12934-017-0646-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084251156", 
              "https://doi.org/10.1186/s12934-017-0646-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.abb.2017.07.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090897737"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-03-26", 
        "datePublishedReg": "2019-03-26", 
        "description": "Coenzyme Q (CoQ) plays an important role as an electron transporter in the respiratory chain. It is formed from a benzoquinone ring and an isoprenoid chain of a specific length depending on the organism. We constructed an engineered Escherichia coli strain (menF) unable to produce demethylmenaquinone and menaquinone, compounds that compete for both chorismate, precursor of the benzoquinone ring, and the isoprenoid chain involved in CoQ biosynthesis. In addition, a mutant strain (entC) unable to produce enterobactin, high-affinity siderophore, synthesized from chorismate, and a double mutant (entC-menF) were constructed. The use of glucose or glycerol as carbon sources was also evaluated for the production of CoQ8 in these strains. The double mutant (entC-menF) showed 18% increase in CoQ8-specific content compared to the control strain; however, the single-mutant strains did not show statistically significant differences in CoQ8-specific content respect to the control, in glucose medium in bioreactor experiments. Glycerol was significantly superior compared to glucose for the production of CoQ8 in E. coli, where the CoQ8-specific content increased 126% and 53% in the control and double-mutant strain, respectively. The expression of genes related to CoQ8 biosynthesis is reported, where the entC-menF double-mutant strain showed a significant increase in the expression of CoQ8 biosynthesis-related genes when glycerol was used as sole carbon source. The control strain did not show gene expression difference between both carbon sources, indicating a possible regulation at a different level.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00449-019-02111-y", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1297453", 
            "issn": [
              "1615-7591", 
              "1615-7605"
            ], 
            "name": "Bioprocess and Biosystems Engineering", 
            "type": "Periodical"
          }
        ], 
        "name": "Coenzyme Q production by metabolic engineered Escherichia coli strains in defined medium", 
        "pagination": "1-7", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "e31244e0f81df4d1f3de12e62d1ed2139c83d53cb30706eff106a63593ed1cbe"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30915537"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101088505"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00449-019-02111-y"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1113011323"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00449-019-02111-y", 
          "https://app.dimensions.ai/details/publication/pub.1113011323"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:11", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000367_0000000367/records_88253_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs00449-019-02111-y"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02111-y'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02111-y'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02111-y'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00449-019-02111-y'


     

    This table displays all metadata directly associated to this object as RDF triples.

    212 TRIPLES      21 PREDICATES      58 URIs      18 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00449-019-02111-y schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author N15444157962a410abc44d2ec8c640980
    4 schema:citation sg:pub.10.1007/978-94-007-5055-5_15
    5 sg:pub.10.1007/s00253-004-1743-y
    6 sg:pub.10.1007/s00253-006-0359-9
    7 sg:pub.10.1007/s00284-004-4417-1
    8 sg:pub.10.1007/s00449-008-0294-5
    9 sg:pub.10.1007/s10295-014-1458-8
    10 sg:pub.10.1007/s10295-015-1652-3
    11 sg:pub.10.1038/nature14559
    12 sg:pub.10.1038/nprot.2008.73
    13 sg:pub.10.1186/s12934-017-0646-4
    14 sg:pub.10.1385/mb:35:1:1
    15 https://app.dimensions.ai/details/publication/pub.1077526900
    16 https://doi.org/10.1002/biof.5520320128
    17 https://doi.org/10.1002/bit.10160
    18 https://doi.org/10.1006/meth.2001.1262
    19 https://doi.org/10.1016/0005-2728(77)90071-8
    20 https://doi.org/10.1016/0922-338x(95)91268-a
    21 https://doi.org/10.1016/j.abb.2017.07.014
    22 https://doi.org/10.1016/j.jbiotec.2009.04.010
    23 https://doi.org/10.1016/j.pharmthera.2009.07.003
    24 https://doi.org/10.1016/j.tibtech.2007.08.008
    25 https://doi.org/10.1016/j.ymben.2004.10.002
    26 https://doi.org/10.1016/j.ymben.2006.05.002
    27 https://doi.org/10.1016/s0014-5793(97)00143-9
    28 https://doi.org/10.1016/s0014-5793(97)01041-7
    29 https://doi.org/10.1016/s0014-5793(99)00360-9
    30 https://doi.org/10.1016/s0378-1097(03)00343-4
    31 https://doi.org/10.1021/cb5008103
    32 https://doi.org/10.1038/msb4100050
    33 https://doi.org/10.1042/ba20090035
    34 https://doi.org/10.1073/pnas.120163297
    35 https://doi.org/10.1271/bbb.58.1814
    36 schema:datePublished 2019-03-26
    37 schema:datePublishedReg 2019-03-26
    38 schema:description Coenzyme Q (CoQ) plays an important role as an electron transporter in the respiratory chain. It is formed from a benzoquinone ring and an isoprenoid chain of a specific length depending on the organism. We constructed an engineered Escherichia coli strain (menF) unable to produce demethylmenaquinone and menaquinone, compounds that compete for both chorismate, precursor of the benzoquinone ring, and the isoprenoid chain involved in CoQ biosynthesis. In addition, a mutant strain (entC) unable to produce enterobactin, high-affinity siderophore, synthesized from chorismate, and a double mutant (entC-menF) were constructed. The use of glucose or glycerol as carbon sources was also evaluated for the production of CoQ<sub>8</sub> in these strains. The double mutant (entC-menF) showed 18% increase in CoQ<sub>8</sub>-specific content compared to the control strain; however, the single-mutant strains did not show statistically significant differences in CoQ<sub>8</sub>-specific content respect to the control, in glucose medium in bioreactor experiments. Glycerol was significantly superior compared to glucose for the production of CoQ<sub>8</sub> in E. coli, where the CoQ<sub>8</sub>-specific content increased 126% and 53% in the control and double-mutant strain, respectively. The expression of genes related to CoQ<sub>8</sub> biosynthesis is reported, where the entC-menF double-mutant strain showed a significant increase in the expression of CoQ<sub>8</sub> biosynthesis-related genes when glycerol was used as sole carbon source. The control strain did not show gene expression difference between both carbon sources, indicating a possible regulation at a different level.
    39 schema:genre research_article
    40 schema:inLanguage en
    41 schema:isAccessibleForFree false
    42 schema:isPartOf sg:journal.1297453
    43 schema:name Coenzyme Q production by metabolic engineered Escherichia coli strains in defined medium
    44 schema:pagination 1-7
    45 schema:productId N0cb039a07918432b8f5d9558e5ce8331
    46 N51f3497b986546aba0579582cc3c5d62
    47 N5851de9a24814a17abc2827971600a03
    48 N8ccb7fbbb4a643a19233c8687a5a9ad3
    49 Nefc83b0baee94f0d95b3757491f5ff74
    50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113011323
    51 https://doi.org/10.1007/s00449-019-02111-y
    52 schema:sdDatePublished 2019-04-11T13:11
    53 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    54 schema:sdPublisher Nd1b0d60c8ea94219945e1d06a2ede2e7
    55 schema:url https://link.springer.com/10.1007%2Fs00449-019-02111-y
    56 sgo:license sg:explorer/license/
    57 sgo:sdDataset articles
    58 rdf:type schema:ScholarlyArticle
    59 N07a97e0d6d284c6a912c287dcd7b3a3b schema:affiliation https://www.grid.ac/institutes/grid.8170.e
    60 schema:familyName Urtuvia
    61 schema:givenName Viviana
    62 rdf:type schema:Person
    63 N0bef4e1a1ed740878d337b710ca561d0 rdf:first N9d6ced15b92b4b2584bb149df6e3c8dd
    64 rdf:rest N742266f1acf94c03873250415ac2bc8e
    65 N0cb039a07918432b8f5d9558e5ce8331 schema:name nlm_unique_id
    66 schema:value 101088505
    67 rdf:type schema:PropertyValue
    68 N13ddb15c24864f64a5045abf3cbe55cb schema:affiliation https://www.grid.ac/institutes/grid.8170.e
    69 schema:familyName Guevara
    70 schema:givenName Felipe
    71 rdf:type schema:Person
    72 N15444157962a410abc44d2ec8c640980 rdf:first N2404e5562f714c559e176c926831d5a8
    73 rdf:rest N0bef4e1a1ed740878d337b710ca561d0
    74 N2404e5562f714c559e176c926831d5a8 schema:affiliation https://www.grid.ac/institutes/grid.8170.e
    75 schema:familyName Martínez
    76 schema:givenName Irene
    77 rdf:type schema:Person
    78 N28136ab373bb4c3ab9cdd9b1f5b83ec5 rdf:first Nb75a6673e8ea4742bf5cc254ef8df915
    79 rdf:rest Nd1afa40f55fe498a8e7e1964adbbfd55
    80 N51f3497b986546aba0579582cc3c5d62 schema:name readcube_id
    81 schema:value e31244e0f81df4d1f3de12e62d1ed2139c83d53cb30706eff106a63593ed1cbe
    82 rdf:type schema:PropertyValue
    83 N5851de9a24814a17abc2827971600a03 schema:name dimensions_id
    84 schema:value pub.1113011323
    85 rdf:type schema:PropertyValue
    86 N742266f1acf94c03873250415ac2bc8e rdf:first N13ddb15c24864f64a5045abf3cbe55cb
    87 rdf:rest N28136ab373bb4c3ab9cdd9b1f5b83ec5
    88 N8694ec81a8914b27b6318651f42094af rdf:first N8e9c47bcbb0a4817a57be7bdb571c1e2
    89 rdf:rest Nfe7e023190034ce39b05a197c77598b0
    90 N8ccb7fbbb4a643a19233c8687a5a9ad3 schema:name pubmed_id
    91 schema:value 30915537
    92 rdf:type schema:PropertyValue
    93 N8e9c47bcbb0a4817a57be7bdb571c1e2 schema:affiliation https://www.grid.ac/institutes/grid.5808.5
    94 schema:familyName Pacheco-Leyva
    95 schema:givenName Ivette
    96 rdf:type schema:Person
    97 N9d6ced15b92b4b2584bb149df6e3c8dd schema:affiliation https://www.grid.ac/institutes/grid.8170.e
    98 schema:familyName Zelada
    99 schema:givenName Patricio
    100 rdf:type schema:Person
    101 Na81bfbd50655421da073758b7eb59800 schema:affiliation https://www.grid.ac/institutes/grid.8170.e
    102 schema:familyName Díaz-Barrera
    103 schema:givenName Alvaro
    104 rdf:type schema:Person
    105 Nb75a6673e8ea4742bf5cc254ef8df915 schema:affiliation https://www.grid.ac/institutes/grid.411964.f
    106 schema:familyName Andler
    107 schema:givenName Rodrigo
    108 rdf:type schema:Person
    109 Nd1afa40f55fe498a8e7e1964adbbfd55 rdf:first N07a97e0d6d284c6a912c287dcd7b3a3b
    110 rdf:rest N8694ec81a8914b27b6318651f42094af
    111 Nd1b0d60c8ea94219945e1d06a2ede2e7 schema:name Springer Nature - SN SciGraph project
    112 rdf:type schema:Organization
    113 Nefc83b0baee94f0d95b3757491f5ff74 schema:name doi
    114 schema:value 10.1007/s00449-019-02111-y
    115 rdf:type schema:PropertyValue
    116 Nfe7e023190034ce39b05a197c77598b0 rdf:first Na81bfbd50655421da073758b7eb59800
    117 rdf:rest rdf:nil
    118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    119 schema:name Biological Sciences
    120 rdf:type schema:DefinedTerm
    121 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    122 schema:name Genetics
    123 rdf:type schema:DefinedTerm
    124 sg:journal.1297453 schema:issn 1615-7591
    125 1615-7605
    126 schema:name Bioprocess and Biosystems Engineering
    127 rdf:type schema:Periodical
    128 sg:pub.10.1007/978-94-007-5055-5_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048528611
    129 https://doi.org/10.1007/978-94-007-5055-5_15
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/s00253-004-1743-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1053132398
    132 https://doi.org/10.1007/s00253-004-1743-y
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/s00253-006-0359-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029607486
    135 https://doi.org/10.1007/s00253-006-0359-9
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1007/s00284-004-4417-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012018465
    138 https://doi.org/10.1007/s00284-004-4417-1
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1007/s00449-008-0294-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028445126
    141 https://doi.org/10.1007/s00449-008-0294-5
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1007/s10295-014-1458-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036808906
    144 https://doi.org/10.1007/s10295-014-1458-8
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/s10295-015-1652-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011758248
    147 https://doi.org/10.1007/s10295-015-1652-3
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1038/nature14559 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003482110
    150 https://doi.org/10.1038/nature14559
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1038/nprot.2008.73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005355981
    153 https://doi.org/10.1038/nprot.2008.73
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1186/s12934-017-0646-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084251156
    156 https://doi.org/10.1186/s12934-017-0646-4
    157 rdf:type schema:CreativeWork
    158 sg:pub.10.1385/mb:35:1:1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033796372
    159 https://doi.org/10.1385/mb:35:1:1
    160 rdf:type schema:CreativeWork
    161 https://app.dimensions.ai/details/publication/pub.1077526900 schema:CreativeWork
    162 https://doi.org/10.1002/biof.5520320128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026105436
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1002/bit.10160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008270581
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1006/meth.2001.1262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027621591
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1016/0005-2728(77)90071-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002351535
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1016/0922-338x(95)91268-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1004156695
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1016/j.abb.2017.07.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090897737
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/j.jbiotec.2009.04.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003234947
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/j.pharmthera.2009.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043853072
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1016/j.tibtech.2007.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002059525
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1016/j.ymben.2004.10.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033219497
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1016/j.ymben.2006.05.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008941575
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1016/s0014-5793(97)00143-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000167444
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1016/s0014-5793(97)01041-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048564892
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1016/s0014-5793(99)00360-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019205919
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1016/s0378-1097(03)00343-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020447832
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1021/cb5008103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055226707
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1038/msb4100050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007788508
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1042/ba20090035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004691527
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1073/pnas.120163297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039276805
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1271/bbb.58.1814 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033070703
    201 rdf:type schema:CreativeWork
    202 https://www.grid.ac/institutes/grid.411964.f schema:alternateName Catholic University of the Maule
    203 schema:name Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Av. San Miguel 3605, Talca, Chile
    204 rdf:type schema:Organization
    205 https://www.grid.ac/institutes/grid.5808.5 schema:alternateName University of Porto
    206 schema:name Biochemical Engineering School, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Casilla 4059,, Valparaíso, Chile
    207 i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal
    208 rdf:type schema:Organization
    209 https://www.grid.ac/institutes/grid.8170.e schema:alternateName Pontificial Catholic University of Valparaiso
    210 schema:name Biochemical Engineering School, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Casilla 4059,, Valparaíso, Chile
    211 Dirección de Investigación y Postgrado, Universidad tecnológica de Chile INACAP, Av. Cóndor Norte 720, Ciudad Empresarial, Huechuraba, Santiago, Chile
    212 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...