Effect of ionic liquids on enzymatic synthesis of caffeic acid phenethyl ester View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-01

AUTHORS

Sung Ho Ha, Tran Van Anh, Sang Hyun Lee, Yoon-Mo Koo

ABSTRACT

Although caffeic acid phenethyl ester (CAPE), an active flavonoid, plays an important role in the antioxidant activity of honeybee propolis, the isolation of CAPE from honeybee propolis is time-consuming due to wide variety of impurities present. Therefore, biochemical method to synthesize CAPE was investigated in this study. Since ionic liquids (ILs) possess some unique characteristics as appreciated alternatives to conventional solvents for certain biotransformation, the effect of ILs as reaction media for enzymatic synthesis of CAPE was assessed. Several factors including substrate molar ratio, and reaction temperature affecting the conversion yield of lipase-catalyzed CAPE synthesis were also investigated. Reaction yields were significantly higher in hydrophobic ILs than in hydrophilic ILs (almost zero). Among nine hydrophobic ILs tested, the highest conversion of synthetic reaction was obtained in 1-ethyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]imide ([Emim][Tf(2)N]). A reaction temperature of 70 °C was found to give high conversion. In addition, optimal substrate molar ratio between phenethyl alcohol and caffeic acid (CA) was decreased significantly from 92:1 to 30:1 when ILs were used instead of isooctane. More... »

PAGES

235-240

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00449-011-0601-4

DOI

http://dx.doi.org/10.1007/s00449-011-0601-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048807677

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21909673


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