Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-08-04

AUTHORS

Renu Singh, Anirban Banerjee, Praveen Kaul, Brajesh Barse, U. C. Banerjee

ABSTRACT

Nitrilases constitute an important class of hydrolases, however, cheap and ready availability of enzyme sources limit their practical synthetic applications. The present investigation was directed to compare the applicability of various physical cell disintegration methods namely, solid shear, liquid shear and sonication, for the release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126. Different parameters associated with each method were optimized in order to ensure maximal release of active nitrilase. The methods were also compared under optimal conditions for their efficiency of nitrilase release and extent of cell disruption, and enzyme release were visualized under a differential interference contrast microscope (DIC) and SDS-PAGE, respectively. Maximum release of the enzyme protein from the cells was observed in case of liquid shear method employing high-pressure homogenization, however, the specific activity of nitrilase was highest in cell-free extract (CFE) generated by sonication. Both the solid shear and liquid shear proved to be equally effective for maximum release of intracellular enzymes, however, from the specific activity point of view, sonication was found to be a better one compared to other two methodologies. The generated cell-free extract can be further employed for the production of enantiopure chiral carboxylic acids, which are important chiral building blocks. More... »

PAGES

415-424

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00449-005-0013-4

DOI

http://dx.doi.org/10.1007/s00449-005-0013-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043476050

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16080003


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211 schema:name Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research, Sector-67, 160 062, SAS Nagar, Punjab, India
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