AFLP fingerprinting of the human genome View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-01

AUTHORS

Michal Prochazka, Ken Walder, James Xia

ABSTRACT

Elucidation of the genetic basis of complex traits and diseases in humans includes the use of genome-wide association studies that depend on the analysis of a large number of diallelic markers. We describe the application of the amplified fragment length polymorphism (AFLP) technique as an efficient approach for rapidly identifying and scoring multiple variants in the human genome. Using a commercially available kit, we found that AFLP yields reproducible DNA fingerprints consisting of 42-132 fragments, 8% of which show variability between individuals. These variant markers appear to be from different chromosomes, and the majority of them is diallelic. Based on the information obtained in this study, it is possible to approximate the minimum number of selective AFLP primer combinations needed to approach a desired coverage density of all chromosomes. To our knowledge, this is the first study showing the general applicability of AFLP in humans and providing a constructive guide for the design of genomic studies in Homo sapiens with this robust methodology. More... »

PAGES

59-65

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004390000438

DOI

http://dx.doi.org/10.1007/s004390000438

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028599077

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11214909


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