In vivo expression of a full-length cDNA copy of potato leafroll virus (PLRV) in protoplasts and transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-02

AUTHORS

D. Prüfer, J. Schmitz, E. Tacke, B. Kull, W. Rohde

ABSTRACT

A full-length cDNA copy (PLRVfl) of potato leafroll virus (PLRV) was constructed and examined in vivo for its biological activities by transient expression experiments with plasmid DNA or in vitro transcribed RNA. In addition, PLRVfl cDNA was stably introduced into the genome of potato plants by Agrobacterium-mediated leaf disc transformation. Both transient and stable expression of PLRVfl resulted in the synthesis of genomic and subgenomic PLRV RNAs. Transgenic plants accumulated the 17-kDa movement protein and displayed the typical symptoms of PLRV infection. This is the first example of the constitutive expression of a phloem-limited virus in planta. More... »

PAGES

609-614

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004380050363

DOI

http://dx.doi.org/10.1007/s004380050363

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051842317

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9065694


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