Isolation and characterization of cDNAs encoding mitochondrial uncoupling proteins in wheat: wheat UCP genes are not regulated by low temperature View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-09

AUTHORS

S. Murayama, H. Handa

ABSTRACT

Uncoupling proteins (UCP) found in the inner mitochondrial membrane of mammals dissipate the proton electrochemical gradient across the inner membrane to produce heat rather than synthesize ATP. Using PCR-based methods, we isolated two novel cDNA clones, WhUCP1a and WhUCP1b, that encode the mitochondrial uncoupling protein of wheat (Triticum aestivum L.). The cDNA clones each contain one ORF which can code for a protein of 286 amino acids with a predicted molecular mass of about 30.5 kDa, although three amino acid substitutions are found between them. The deduced amino acid sequences each possess three typical mitochondrial carrier signature domains and six membrane-spanning domains which are highly conserved in the mitochondrial transporter family. Southern analysis suggested that the WhUCP1 gene may be present in as many as three copies in the wheat genome, and also that WhUCP proteins may be encoded by a small multigene family. Northern analysis revealed that the steady-state level of the WhUCP1 mRNA is quite low. Quantitative RT-PCR clearly showed that expression of the WhUCP1 gene in wheat seedlings is insensitive to low temperature. Our data suggest that WhUCP1 might have functions other than low temperature-induced thermogenesis, although WhUCP1 possesses all the typical features reported for known UCPs. More... »

PAGES

112-118

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004380000289

DOI

http://dx.doi.org/10.1007/s004380000289

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042319997

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11016840


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Southern", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Complementary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ion Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitochondria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitochondrial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Triticum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Uncoupling Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Uncoupling Protein 1", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Agricultural Research Center for Hokkaido Region", 
          "id": "https://www.grid.ac/institutes/grid.419106.b", 
          "name": [
            "Laboratory of Plant Genecology, Hokkaido National Agricultural Experiment Station, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo, 062-8555, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murayama", 
        "givenName": "S.", 
        "id": "sg:person.01321141507.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321141507.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Agricultural Research Center for Hokkaido Region", 
          "id": "https://www.grid.ac/institutes/grid.419106.b", 
          "name": [
            "Laboratory of Plant Genecology, Hokkaido National Agricultural Experiment Station, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo, 062-8555, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Handa", 
        "givenName": "H.", 
        "id": "sg:person.0712021774.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712021774.43"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2000-09", 
    "datePublishedReg": "2000-09-01", 
    "description": "Uncoupling proteins (UCP) found in the inner mitochondrial membrane of mammals dissipate the proton electrochemical gradient across the inner membrane to produce heat rather than synthesize ATP. Using PCR-based methods, we isolated two novel cDNA clones, WhUCP1a and WhUCP1b, that encode the mitochondrial uncoupling protein of wheat (Triticum aestivum L.). The cDNA clones each contain one ORF which can code for a protein of 286 amino acids with a predicted molecular mass of about 30.5 kDa, although three amino acid substitutions are found between them. The deduced amino acid sequences each possess three typical mitochondrial carrier signature domains and six membrane-spanning domains which are highly conserved in the mitochondrial transporter family. Southern analysis suggested that the WhUCP1 gene may be present in as many as three copies in the wheat genome, and also that WhUCP proteins may be encoded by a small multigene family. Northern analysis revealed that the steady-state level of the WhUCP1 mRNA is quite low. Quantitative RT-PCR clearly showed that expression of the WhUCP1 gene in wheat seedlings is insensitive to low temperature. Our data suggest that WhUCP1 might have functions other than low temperature-induced thermogenesis, although WhUCP1 possesses all the typical features reported for known UCPs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s004380000289", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "264"
      }
    ], 
    "name": "Isolation and characterization of cDNAs encoding mitochondrial uncoupling proteins in wheat: wheat UCP genes are not regulated by low temperature", 
    "pagination": "112-118", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c996d16a2978af77157c3226f4e6111d8581d55e51a73b5e35d1122eec5b114f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11016840"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0125036"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s004380000289"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1042319997"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s004380000289", 
      "https://app.dimensions.ai/details/publication/pub.1042319997"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113644_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs004380000289"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s004380000289'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s004380000289'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s004380000289'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s004380000289'


 

This table displays all metadata directly associated to this object as RDF triples.

140 TRIPLES      20 PREDICATES      45 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s004380000289 schema:about N01936103a3354c52827eecb176ecb9fa
2 N4b4c5acc5d5f409a9ce5beb7897022dd
3 N5e5f2157314f4a8188d0574855404a06
4 N5efd6008c609486a8c0758e85ec269e9
5 N61a87cc947e140f9973cdf7fc6425173
6 N6dcfc02f489c48928fae24922705476f
7 N73686a0ca068405b8eb9e07192d7f70f
8 N8b6e4aead9d5438e92bc3ebfa4e51d90
9 N9291842fe7db4ba49d669cbfbac5bd74
10 Naa37561182e14896a07a70f47bf0524b
11 Nb362727365674311a886bde6eb08ffbf
12 Nb6454dc970574694af1bd980e5cc0dc9
13 Nb97d1ba36a4f49179a17274cb7c9d2a1
14 Nc652a221692d444ca2521e37dff98311
15 Nd4bd0f83c7694f499cdbe9aecee5b2f2
16 Ndabe627cd11f4c339a022c4efa2964de
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author N55b726fecad741919243b6367482535d
20 schema:datePublished 2000-09
21 schema:datePublishedReg 2000-09-01
22 schema:description Uncoupling proteins (UCP) found in the inner mitochondrial membrane of mammals dissipate the proton electrochemical gradient across the inner membrane to produce heat rather than synthesize ATP. Using PCR-based methods, we isolated two novel cDNA clones, WhUCP1a and WhUCP1b, that encode the mitochondrial uncoupling protein of wheat (Triticum aestivum L.). The cDNA clones each contain one ORF which can code for a protein of 286 amino acids with a predicted molecular mass of about 30.5 kDa, although three amino acid substitutions are found between them. The deduced amino acid sequences each possess three typical mitochondrial carrier signature domains and six membrane-spanning domains which are highly conserved in the mitochondrial transporter family. Southern analysis suggested that the WhUCP1 gene may be present in as many as three copies in the wheat genome, and also that WhUCP proteins may be encoded by a small multigene family. Northern analysis revealed that the steady-state level of the WhUCP1 mRNA is quite low. Quantitative RT-PCR clearly showed that expression of the WhUCP1 gene in wheat seedlings is insensitive to low temperature. Our data suggest that WhUCP1 might have functions other than low temperature-induced thermogenesis, although WhUCP1 possesses all the typical features reported for known UCPs.
23 schema:genre research_article
24 schema:inLanguage en
25 schema:isAccessibleForFree false
26 schema:isPartOf N1a34bcc17ef44d6ca1702cb2362a5423
27 N90649fb3f186421f85d378b8d392426b
28 sg:journal.1297380
29 schema:name Isolation and characterization of cDNAs encoding mitochondrial uncoupling proteins in wheat: wheat UCP genes are not regulated by low temperature
30 schema:pagination 112-118
31 schema:productId N28d9d383d8c142b18575fceee55c5bd1
32 Nb1870afa66df4965bb8c9107f3b1637e
33 Ne2ef5c6129b54105ad90e74a7a682768
34 Nec2c0e861cc747f2b7318d4eb38779ac
35 Nf464f25abcf347d4bc4414c517b19ba5
36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042319997
37 https://doi.org/10.1007/s004380000289
38 schema:sdDatePublished 2019-04-11T10:29
39 schema:sdLicense https://scigraph.springernature.com/explorer/license/
40 schema:sdPublisher N5c487d1e656f47aab4978a6ff618324b
41 schema:url https://link.springer.com/10.1007%2Fs004380000289
42 sgo:license sg:explorer/license/
43 sgo:sdDataset articles
44 rdf:type schema:ScholarlyArticle
45 N01936103a3354c52827eecb176ecb9fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
46 schema:name Gene Expression Regulation, Plant
47 rdf:type schema:DefinedTerm
48 N1a34bcc17ef44d6ca1702cb2362a5423 schema:volumeNumber 264
49 rdf:type schema:PublicationVolume
50 N28d9d383d8c142b18575fceee55c5bd1 schema:name doi
51 schema:value 10.1007/s004380000289
52 rdf:type schema:PropertyValue
53 N4b4c5acc5d5f409a9ce5beb7897022dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Membrane Proteins
55 rdf:type schema:DefinedTerm
56 N55b726fecad741919243b6367482535d rdf:first sg:person.01321141507.25
57 rdf:rest Nc20f278968e64d64b0664308b7d81ab5
58 N5c487d1e656f47aab4978a6ff618324b schema:name Springer Nature - SN SciGraph project
59 rdf:type schema:Organization
60 N5e5f2157314f4a8188d0574855404a06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Mitochondria
62 rdf:type schema:DefinedTerm
63 N5efd6008c609486a8c0758e85ec269e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Uncoupling Agents
65 rdf:type schema:DefinedTerm
66 N61a87cc947e140f9973cdf7fc6425173 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Molecular Sequence Data
68 rdf:type schema:DefinedTerm
69 N6dcfc02f489c48928fae24922705476f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Blotting, Southern
71 rdf:type schema:DefinedTerm
72 N73686a0ca068405b8eb9e07192d7f70f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Amino Acid Sequence
74 rdf:type schema:DefinedTerm
75 N8b6e4aead9d5438e92bc3ebfa4e51d90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Plant Proteins
77 rdf:type schema:DefinedTerm
78 N90649fb3f186421f85d378b8d392426b schema:issueNumber 1-2
79 rdf:type schema:PublicationIssue
80 N9291842fe7db4ba49d669cbfbac5bd74 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Cloning, Molecular
82 rdf:type schema:DefinedTerm
83 Naa37561182e14896a07a70f47bf0524b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Ion Channels
85 rdf:type schema:DefinedTerm
86 Nb1870afa66df4965bb8c9107f3b1637e schema:name pubmed_id
87 schema:value 11016840
88 rdf:type schema:PropertyValue
89 Nb362727365674311a886bde6eb08ffbf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Triticum
91 rdf:type schema:DefinedTerm
92 Nb6454dc970574694af1bd980e5cc0dc9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Sequence Homology, Amino Acid
94 rdf:type schema:DefinedTerm
95 Nb97d1ba36a4f49179a17274cb7c9d2a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Temperature
97 rdf:type schema:DefinedTerm
98 Nc20f278968e64d64b0664308b7d81ab5 rdf:first sg:person.0712021774.43
99 rdf:rest rdf:nil
100 Nc652a221692d444ca2521e37dff98311 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Mitochondrial Proteins
102 rdf:type schema:DefinedTerm
103 Nd4bd0f83c7694f499cdbe9aecee5b2f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name DNA, Complementary
105 rdf:type schema:DefinedTerm
106 Ndabe627cd11f4c339a022c4efa2964de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Uncoupling Protein 1
108 rdf:type schema:DefinedTerm
109 Ne2ef5c6129b54105ad90e74a7a682768 schema:name dimensions_id
110 schema:value pub.1042319997
111 rdf:type schema:PropertyValue
112 Nec2c0e861cc747f2b7318d4eb38779ac schema:name readcube_id
113 schema:value c996d16a2978af77157c3226f4e6111d8581d55e51a73b5e35d1122eec5b114f
114 rdf:type schema:PropertyValue
115 Nf464f25abcf347d4bc4414c517b19ba5 schema:name nlm_unique_id
116 schema:value 0125036
117 rdf:type schema:PropertyValue
118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biological Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biochemistry and Cell Biology
123 rdf:type schema:DefinedTerm
124 sg:journal.1297380 schema:issn 1432-1874
125 1617-4615
126 schema:name Molecular Genetics and Genomics
127 rdf:type schema:Periodical
128 sg:person.01321141507.25 schema:affiliation https://www.grid.ac/institutes/grid.419106.b
129 schema:familyName Murayama
130 schema:givenName S.
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321141507.25
132 rdf:type schema:Person
133 sg:person.0712021774.43 schema:affiliation https://www.grid.ac/institutes/grid.419106.b
134 schema:familyName Handa
135 schema:givenName H.
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0712021774.43
137 rdf:type schema:Person
138 https://www.grid.ac/institutes/grid.419106.b schema:alternateName National Agricultural Research Center for Hokkaido Region
139 schema:name Laboratory of Plant Genecology, Hokkaido National Agricultural Experiment Station, 1, Hitsuji-ga-oka, Toyohira-ku, Sapporo, 062-8555, Japan
140 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...