Use of the transposable element Ac/Ds in conjunction with Spm/dSpm for gene tagging allows extensive genome coverage with a limited ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-12

AUTHORS

Priya Panjabi, Pradeep Kumar Burma, Deepak Pental

ABSTRACT

We have developed a novel four-element based gene tagging system in Arabidopsis to minimize the number of starter lines required to generate genome-wide insertions for saturation mutagenesis. In this system, the non-autonomous cassette, Ds(dSpm), comprises of both Ds and dSpm elements cloned one within the other along with appropriate selection markers to allow efficient monitoring of excision and re-integration of the transposons. Trans-activation of the outer borders (Ds) and selection against the negative selection marker (iaaH) linked to the cassette ensures unlinked spread of the Ds(dSpm) cassette from the initial site of integration of the T-DNA. This creates several launch pads within the genome from where the internal element (dSpm) can be subsequently mobilized to generate secondary insertions. In this study, starting from a single T-DNA integration we could spread the Ds(dSpm) cassette to 11 different locations over all the five chromosomes of Arabidopsis. The frequency of unlinked Ds transpositions in the F2 generation varied between 0.05 and 3.35%. Three of these lines were then deployed to trans-activate the internal dSpm element which led to the selection of 29 dSpm insertions. The study conclusively shows the feasibility of deploying Ds and the dSpm elements in a single construct for insertional mutagenesis. More... »

PAGES

533-543

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00438-006-0158-0

DOI

http://dx.doi.org/10.1007/s00438-006-0158-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043645199

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17004082


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arabidopsis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Transposable Elements", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Delhi", 
          "id": "https://www.grid.ac/institutes/grid.8195.5", 
          "name": [
            "Department of Genetics, University of Delhi South Campus, Benito Juarez Road, 110021, New Delhi, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Panjabi", 
        "givenName": "Priya", 
        "id": "sg:person.0744045565.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744045565.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Delhi", 
          "id": "https://www.grid.ac/institutes/grid.8195.5", 
          "name": [
            "Department of Genetics, University of Delhi South Campus, Benito Juarez Road, 110021, New Delhi, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Burma", 
        "givenName": "Pradeep Kumar", 
        "id": "sg:person.01301675410.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301675410.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Delhi", 
          "id": "https://www.grid.ac/institutes/grid.8195.5", 
          "name": [
            "Department of Genetics, University of Delhi South Campus, Benito Juarez Road, 110021, New Delhi, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pental", 
        "givenName": "Deepak", 
        "id": "sg:person.01230565713.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230565713.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00265443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000158433", 
          "https://doi.org/10.1007/bf00265443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00265443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000158433", 
          "https://doi.org/10.1007/bf00265443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1996.10040721.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001534173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00279905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001761137", 
          "https://doi.org/10.1007/bf00279905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00279905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001761137", 
          "https://doi.org/10.1007/bf00279905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00330942", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005055904", 
          "https://doi.org/10.1007/bf00330942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00014672", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007506198", 
          "https://doi.org/10.1007/bf00014672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00014672", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007506198", 
          "https://doi.org/10.1007/bf00014672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00021428", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009316145", 
          "https://doi.org/10.1007/bf00021428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00021428", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009316145", 
          "https://doi.org/10.1007/bf00021428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.002774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011036962"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00229496", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012423870", 
          "https://doi.org/10.1007/bf00229496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00229496", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012423870", 
          "https://doi.org/10.1007/bf00229496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1995.08030457.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013034175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006455130098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014682200", 
          "https://doi.org/10.1023/a:1006455130098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02985182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018934216", 
          "https://doi.org/10.1007/bf02985182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02985182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018934216", 
          "https://doi.org/10.1007/bf02985182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02985182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018934216", 
          "https://doi.org/10.1007/bf02985182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0981-9428(01)01243-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019529277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-011-0511-8_12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020790088", 
          "https://doi.org/10.1007/978-94-011-0511-8_12"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.11.10.1841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021421938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.003327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022011309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.2005.02570.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022653012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.2005.02570.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022653012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.123.2.439", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023566091"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00438-003-0942-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025909937", 
          "https://doi.org/10.1007/s00438-003-0942-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.2000.00786.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026048630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.11.12.2263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026501829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.1993.tb00169.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026946009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0888-7543(95)80010-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027027849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0888-7543(95)80010-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027027849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.8.4.659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027138034"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.7.2219", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029405697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.977903", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031498613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.2001.01084.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032806855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.7.6.735", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032849777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.2003.01723.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034685098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036333064", 
          "https://doi.org/10.1038/nbt1043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036333064", 
          "https://doi.org/10.1038/nbt1043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1996.10030479.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037667445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00259620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037779307", 
          "https://doi.org/10.1007/bf00259620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00259620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037779307", 
          "https://doi.org/10.1007/bf00259620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.2003.01914.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038613407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.104.050633", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049760501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.9.14.1797", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050453984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.4.5.583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051221964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3870175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3870342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3870953", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3871081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/02334st07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075179848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082789568", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-12", 
    "datePublishedReg": "2006-12-01", 
    "description": "We have developed a novel four-element based gene tagging system in Arabidopsis to minimize the number of starter lines required to generate genome-wide insertions for saturation mutagenesis. In this system, the non-autonomous cassette, Ds(dSpm), comprises of both Ds and dSpm elements cloned one within the other along with appropriate selection markers to allow efficient monitoring of excision and re-integration of the transposons. Trans-activation of the outer borders (Ds) and selection against the negative selection marker (iaaH) linked to the cassette ensures unlinked spread of the Ds(dSpm) cassette from the initial site of integration of the T-DNA. This creates several launch pads within the genome from where the internal element (dSpm) can be subsequently mobilized to generate secondary insertions. In this study, starting from a single T-DNA integration we could spread the Ds(dSpm) cassette to 11 different locations over all the five chromosomes of Arabidopsis. The frequency of unlinked Ds transpositions in the F2 generation varied between 0.05 and 3.35%. Three of these lines were then deployed to trans-activate the internal dSpm element which led to the selection of 29 dSpm insertions. The study conclusively shows the feasibility of deploying Ds and the dSpm elements in a single construct for insertional mutagenesis.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00438-006-0158-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "276"
      }
    ], 
    "name": "Use of the transposable element Ac/Ds in conjunction with Spm/dSpm for gene tagging allows extensive genome coverage with a limited number of starter lines: functional analysis of a four-element system in Arabidopsis thaliana", 
    "pagination": "533-543", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "cfe5b298b640622641c78177e49dd6a63691d6dd3ba1bd2886d58623406b32bd"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17004082"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101093320"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00438-006-0158-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1043645199"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00438-006-0158-0", 
      "https://app.dimensions.ai/details/publication/pub.1043645199"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13081_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00438-006-0158-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0158-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0158-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0158-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0158-0'


 

This table displays all metadata directly associated to this object as RDF triples.

248 TRIPLES      21 PREDICATES      78 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00438-006-0158-0 schema:about N2481c9dbb546481b924748d0eb8cb36b
2 N3df8a5290c5546518e91e2c454922801
3 N3e9cf15394ea4eebb0cac665ea772424
4 N6233e146b81d485f9fb1b6805ed382bb
5 N7b6e9bb8a93d45f89739ff80076f620c
6 Na5f224f89981416f811abfe8841c1e3b
7 Nfe7b4fdddd4a4d99b8a99dacf68175fb
8 anzsrc-for:06
9 anzsrc-for:0604
10 schema:author N5927922f32d94f16b8427037414ff517
11 schema:citation sg:pub.10.1007/978-94-011-0511-8_12
12 sg:pub.10.1007/bf00014672
13 sg:pub.10.1007/bf00021428
14 sg:pub.10.1007/bf00229496
15 sg:pub.10.1007/bf00259620
16 sg:pub.10.1007/bf00265443
17 sg:pub.10.1007/bf00279905
18 sg:pub.10.1007/bf00330942
19 sg:pub.10.1007/bf02985182
20 sg:pub.10.1007/s00438-003-0942-z
21 sg:pub.10.1023/a:1006455130098
22 sg:pub.10.1038/nbt1043
23 https://app.dimensions.ai/details/publication/pub.1082789568
24 https://doi.org/10.1016/0888-7543(95)80010-j
25 https://doi.org/10.1016/s0981-9428(01)01243-8
26 https://doi.org/10.1046/j.1365-313x.1995.08030457.x
27 https://doi.org/10.1046/j.1365-313x.1996.10030479.x
28 https://doi.org/10.1046/j.1365-313x.1996.10040721.x
29 https://doi.org/10.1046/j.1365-313x.2000.00786.x
30 https://doi.org/10.1046/j.1365-313x.2001.01084.x
31 https://doi.org/10.1046/j.1365-313x.2003.01723.x
32 https://doi.org/10.1046/j.1365-313x.2003.01914.x
33 https://doi.org/10.1073/pnas.86.7.2219
34 https://doi.org/10.1101/gad.9.14.1797
35 https://doi.org/10.1101/gr.977903
36 https://doi.org/10.1104/pp.002774
37 https://doi.org/10.1104/pp.003327
38 https://doi.org/10.1104/pp.104.050633
39 https://doi.org/10.1104/pp.123.2.439
40 https://doi.org/10.1105/tpc.11.10.1841
41 https://doi.org/10.1105/tpc.11.12.2263
42 https://doi.org/10.1105/tpc.4.5.583
43 https://doi.org/10.1105/tpc.7.6.735
44 https://doi.org/10.1105/tpc.8.4.659
45 https://doi.org/10.1111/j.1365-313x.1993.tb00169.x
46 https://doi.org/10.1111/j.1365-313x.2005.02570.x
47 https://doi.org/10.2144/02334st07
48 https://doi.org/10.2307/3869557
49 https://doi.org/10.2307/3870175
50 https://doi.org/10.2307/3870342
51 https://doi.org/10.2307/3870953
52 https://doi.org/10.2307/3871081
53 schema:datePublished 2006-12
54 schema:datePublishedReg 2006-12-01
55 schema:description We have developed a novel four-element based gene tagging system in Arabidopsis to minimize the number of starter lines required to generate genome-wide insertions for saturation mutagenesis. In this system, the non-autonomous cassette, Ds(dSpm), comprises of both Ds and dSpm elements cloned one within the other along with appropriate selection markers to allow efficient monitoring of excision and re-integration of the transposons. Trans-activation of the outer borders (Ds) and selection against the negative selection marker (iaaH) linked to the cassette ensures unlinked spread of the Ds(dSpm) cassette from the initial site of integration of the T-DNA. This creates several launch pads within the genome from where the internal element (dSpm) can be subsequently mobilized to generate secondary insertions. In this study, starting from a single T-DNA integration we could spread the Ds(dSpm) cassette to 11 different locations over all the five chromosomes of Arabidopsis. The frequency of unlinked Ds transpositions in the F2 generation varied between 0.05 and 3.35%. Three of these lines were then deployed to trans-activate the internal dSpm element which led to the selection of 29 dSpm insertions. The study conclusively shows the feasibility of deploying Ds and the dSpm elements in a single construct for insertional mutagenesis.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree false
59 schema:isPartOf N4a8c3176896043b8954d6d8fb495e156
60 Ne051e7b6d61f449cb4e5975acdbc6a5b
61 sg:journal.1297380
62 schema:name Use of the transposable element Ac/Ds in conjunction with Spm/dSpm for gene tagging allows extensive genome coverage with a limited number of starter lines: functional analysis of a four-element system in Arabidopsis thaliana
63 schema:pagination 533-543
64 schema:productId N2434d6fcc6604c48900de422878db24f
65 N2b37257c8dbf4c97be12a318379971a8
66 N54b41903d27843008a1333dfd97b7385
67 N656dac58f42f4940b38226c05035edd0
68 Nba366400d81b47fda7b474db381dd134
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043645199
70 https://doi.org/10.1007/s00438-006-0158-0
71 schema:sdDatePublished 2019-04-11T14:28
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher Nc7427812657b4bbd96316de94f6d633b
74 schema:url https://link.springer.com/10.1007%2Fs00438-006-0158-0
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N2434d6fcc6604c48900de422878db24f schema:name nlm_unique_id
79 schema:value 101093320
80 rdf:type schema:PropertyValue
81 N2481c9dbb546481b924748d0eb8cb36b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name DNA Transposable Elements
83 rdf:type schema:DefinedTerm
84 N2b37257c8dbf4c97be12a318379971a8 schema:name pubmed_id
85 schema:value 17004082
86 rdf:type schema:PropertyValue
87 N3df8a5290c5546518e91e2c454922801 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Chromosomes, Plant
89 rdf:type schema:DefinedTerm
90 N3e9cf15394ea4eebb0cac665ea772424 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Genomics
92 rdf:type schema:DefinedTerm
93 N4a8c3176896043b8954d6d8fb495e156 schema:volumeNumber 276
94 rdf:type schema:PublicationVolume
95 N4af09882cdb8416bae65f014c1fe0b8e rdf:first sg:person.01230565713.24
96 rdf:rest rdf:nil
97 N54b41903d27843008a1333dfd97b7385 schema:name doi
98 schema:value 10.1007/s00438-006-0158-0
99 rdf:type schema:PropertyValue
100 N561bc927f97f4a978bd289b7829a432d rdf:first sg:person.01301675410.41
101 rdf:rest N4af09882cdb8416bae65f014c1fe0b8e
102 N5927922f32d94f16b8427037414ff517 rdf:first sg:person.0744045565.19
103 rdf:rest N561bc927f97f4a978bd289b7829a432d
104 N6233e146b81d485f9fb1b6805ed382bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Genetic Vectors
106 rdf:type schema:DefinedTerm
107 N656dac58f42f4940b38226c05035edd0 schema:name dimensions_id
108 schema:value pub.1043645199
109 rdf:type schema:PropertyValue
110 N7b6e9bb8a93d45f89739ff80076f620c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name DNA Primers
112 rdf:type schema:DefinedTerm
113 Na5f224f89981416f811abfe8841c1e3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Arabidopsis
115 rdf:type schema:DefinedTerm
116 Nba366400d81b47fda7b474db381dd134 schema:name readcube_id
117 schema:value cfe5b298b640622641c78177e49dd6a63691d6dd3ba1bd2886d58623406b32bd
118 rdf:type schema:PropertyValue
119 Nc7427812657b4bbd96316de94f6d633b schema:name Springer Nature - SN SciGraph project
120 rdf:type schema:Organization
121 Ne051e7b6d61f449cb4e5975acdbc6a5b schema:issueNumber 6
122 rdf:type schema:PublicationIssue
123 Nfe7b4fdddd4a4d99b8a99dacf68175fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Mutagenesis
125 rdf:type schema:DefinedTerm
126 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
127 schema:name Biological Sciences
128 rdf:type schema:DefinedTerm
129 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
130 schema:name Genetics
131 rdf:type schema:DefinedTerm
132 sg:journal.1297380 schema:issn 1432-1874
133 1617-4615
134 schema:name Molecular Genetics and Genomics
135 rdf:type schema:Periodical
136 sg:person.01230565713.24 schema:affiliation https://www.grid.ac/institutes/grid.8195.5
137 schema:familyName Pental
138 schema:givenName Deepak
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230565713.24
140 rdf:type schema:Person
141 sg:person.01301675410.41 schema:affiliation https://www.grid.ac/institutes/grid.8195.5
142 schema:familyName Burma
143 schema:givenName Pradeep Kumar
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301675410.41
145 rdf:type schema:Person
146 sg:person.0744045565.19 schema:affiliation https://www.grid.ac/institutes/grid.8195.5
147 schema:familyName Panjabi
148 schema:givenName Priya
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744045565.19
150 rdf:type schema:Person
151 sg:pub.10.1007/978-94-011-0511-8_12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020790088
152 https://doi.org/10.1007/978-94-011-0511-8_12
153 rdf:type schema:CreativeWork
154 sg:pub.10.1007/bf00014672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007506198
155 https://doi.org/10.1007/bf00014672
156 rdf:type schema:CreativeWork
157 sg:pub.10.1007/bf00021428 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009316145
158 https://doi.org/10.1007/bf00021428
159 rdf:type schema:CreativeWork
160 sg:pub.10.1007/bf00229496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012423870
161 https://doi.org/10.1007/bf00229496
162 rdf:type schema:CreativeWork
163 sg:pub.10.1007/bf00259620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037779307
164 https://doi.org/10.1007/bf00259620
165 rdf:type schema:CreativeWork
166 sg:pub.10.1007/bf00265443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000158433
167 https://doi.org/10.1007/bf00265443
168 rdf:type schema:CreativeWork
169 sg:pub.10.1007/bf00279905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001761137
170 https://doi.org/10.1007/bf00279905
171 rdf:type schema:CreativeWork
172 sg:pub.10.1007/bf00330942 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005055904
173 https://doi.org/10.1007/bf00330942
174 rdf:type schema:CreativeWork
175 sg:pub.10.1007/bf02985182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018934216
176 https://doi.org/10.1007/bf02985182
177 rdf:type schema:CreativeWork
178 sg:pub.10.1007/s00438-003-0942-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1025909937
179 https://doi.org/10.1007/s00438-003-0942-z
180 rdf:type schema:CreativeWork
181 sg:pub.10.1023/a:1006455130098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014682200
182 https://doi.org/10.1023/a:1006455130098
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/nbt1043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036333064
185 https://doi.org/10.1038/nbt1043
186 rdf:type schema:CreativeWork
187 https://app.dimensions.ai/details/publication/pub.1082789568 schema:CreativeWork
188 https://doi.org/10.1016/0888-7543(95)80010-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1027027849
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/s0981-9428(01)01243-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019529277
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1046/j.1365-313x.1995.08030457.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013034175
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1046/j.1365-313x.1996.10030479.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037667445
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1046/j.1365-313x.1996.10040721.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1001534173
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1046/j.1365-313x.2000.00786.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026048630
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1046/j.1365-313x.2001.01084.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032806855
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1046/j.1365-313x.2003.01723.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034685098
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1046/j.1365-313x.2003.01914.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038613407
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1073/pnas.86.7.2219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029405697
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1101/gad.9.14.1797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050453984
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1101/gr.977903 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031498613
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1104/pp.002774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011036962
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1104/pp.003327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022011309
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1104/pp.104.050633 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049760501
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1104/pp.123.2.439 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023566091
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1105/tpc.11.10.1841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021421938
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1105/tpc.11.12.2263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026501829
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1105/tpc.4.5.583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051221964
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1105/tpc.7.6.735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032849777
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1105/tpc.8.4.659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027138034
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1111/j.1365-313x.1993.tb00169.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026946009
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1111/j.1365-313x.2005.02570.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022653012
233 rdf:type schema:CreativeWork
234 https://doi.org/10.2144/02334st07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075179848
235 rdf:type schema:CreativeWork
236 https://doi.org/10.2307/3869557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467559
237 rdf:type schema:CreativeWork
238 https://doi.org/10.2307/3870175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468100
239 rdf:type schema:CreativeWork
240 https://doi.org/10.2307/3870342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468249
241 rdf:type schema:CreativeWork
242 https://doi.org/10.2307/3870953 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468782
243 rdf:type schema:CreativeWork
244 https://doi.org/10.2307/3871081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468896
245 rdf:type schema:CreativeWork
246 https://www.grid.ac/institutes/grid.8195.5 schema:alternateName University of Delhi
247 schema:name Department of Genetics, University of Delhi South Campus, Benito Juarez Road, 110021, New Delhi, India
248 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...