The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-04

AUTHORS

Lionel Cladière, Kassem Hamze, Edwige Madec, Vladimir M. Levdikov, Anthony J. Wilkinson, I. Barry Holland, Simone J. Séror

ABSTRACT

YloQ, from Bacillus subtilis, was identified previously as an essential nucleotide-binding protein of unknown function. YloQ was successfully over-expressed in Escherichia coli in soluble form. The purified protein displayed a low GTPase activity similar to that of other small bacterial GTPases such as Bex/Era. Based on the demonstrated GTPase activity and the unusual order of the yloQ G motifs, we now designate this protein as CpgA (circularly permuted GTPase). An unexpected property of this low abundance GTPase was the demonstration, using gel filtration and ultracentrifugation analysis, that the protein formed stable dimers, dependent upon the concentration of YloQ(CpgA), but independent of GTP. In order to investigate function, cpgA was placed under the control of the pspac promotor in the B. subtilis chromosome. When grown in E or Spizizen medium in the absence of IPTG, the rate of growth was significantly reduced. A large proportion of the cells exhibited a markedly perturbed morphology, with the formation of swollen, bent or 'curly' shapes. To confirm that this was specifically due to depleted CpgA a plasmid-borne cpgA under pxyl control was introduced. This restored normal cell shape and growth rate, even in the absence of IPTG, provided xylose was present. The crystal structure of CpgA(YloQ) suggests a role as a translation initiation factor and we discuss the possibility that CpgA is involved in the translation of a subset of proteins, including some required for shape maintenance. More... »

PAGES

409-420

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00438-006-0097-9

DOI

http://dx.doi.org/10.1007/s00438-006-0097-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016197900

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16485133


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Motifs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacillus subtilis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Wall", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Conserved Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dimerization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "GTP Phosphohydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Isopropyl Thiogalactoside", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Morphogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multigene Family", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoprotein Phosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Prokaryotic Initiation Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institut de G\u00e9n\u00e9tique et Microbiologie, Universit\u00e9 Paris-Sud, UMR CNRS 8621, B\u00e2timent 409, 91405, Orsay Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cladi\u00e8re", 
        "givenName": "Lionel", 
        "id": "sg:person.01021617122.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021617122.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institut de G\u00e9n\u00e9tique et Microbiologie, Universit\u00e9 Paris-Sud, UMR CNRS 8621, B\u00e2timent 409, 91405, Orsay Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hamze", 
        "givenName": "Kassem", 
        "id": "sg:person.0646102556.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646102556.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institut de G\u00e9n\u00e9tique et Microbiologie, Universit\u00e9 Paris-Sud, UMR CNRS 8621, B\u00e2timent 409, 91405, Orsay Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Madec", 
        "givenName": "Edwige", 
        "id": "sg:person.01352772464.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352772464.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of York", 
          "id": "https://www.grid.ac/institutes/grid.5685.e", 
          "name": [
            "Structural Biology Laboratory, Department of Chemistry, University of York, YO10, York, SYW, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Levdikov", 
        "givenName": "Vladimir M.", 
        "id": "sg:person.0601464400.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601464400.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of York", 
          "id": "https://www.grid.ac/institutes/grid.5685.e", 
          "name": [
            "Structural Biology Laboratory, Department of Chemistry, University of York, YO10, York, SYW, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wilkinson", 
        "givenName": "Anthony J.", 
        "id": "sg:person.01135131247.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135131247.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institut de G\u00e9n\u00e9tique et Microbiologie, Universit\u00e9 Paris-Sud, UMR CNRS 8621, B\u00e2timent 409, 91405, Orsay Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Holland", 
        "givenName": "I. Barry", 
        "id": "sg:person.0724310230.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724310230.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Integrative Biology of the Cell", 
          "id": "https://www.grid.ac/institutes/grid.462411.4", 
          "name": [
            "Institut de G\u00e9n\u00e9tique et Microbiologie, Universit\u00e9 Paris-Sud, UMR CNRS 8621, B\u00e2timent 409, 91405, Orsay Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "S\u00e9ror", 
        "givenName": "Simone J.", 
        "id": "sg:person.01227030231.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227030231.00"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nature02250", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002922834", 
          "https://doi.org/10.1038/nature02250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02250", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002922834", 
          "https://doi.org/10.1038/nature02250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.182.19.5634-5638.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004185665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1369-5274(03)00037-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006496719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1369-5274(03)00037-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006496719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0396-315", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007123993", 
          "https://doi.org/10.1038/nbt0396-315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.186.11.3660-3662.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009174074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(03)00579-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010893831"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(03)00579-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010893831"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2004.05.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012856629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00203-005-0015-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013782862", 
          "https://doi.org/10.1007/s00203-005-0015-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00203-005-0015-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013782862", 
          "https://doi.org/10.1007/s00203-005-0015-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(00)76713-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014356043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(79)90115-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016788675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2003.03475.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024348638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0998-851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027411938", 
          "https://doi.org/10.1038/nbt0998-851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0998-851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027411938", 
          "https://doi.org/10.1038/nbt0998-851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkh861", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029836522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/36786", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031054310", 
          "https://doi.org/10.1038/36786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/36786", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031054310", 
          "https://doi.org/10.1038/36786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.67.1.403-410.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032274862"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20050733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033214675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20050733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033214675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0730515100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033908509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.186.5.1381-1387.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034149426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2004.04343.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034744741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2004.04050.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037148569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2002.03178.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041486871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2003.09.079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042341661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.66.1.639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044366676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20041873", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046123029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20041873", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046123029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0405202101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046123730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(83)80284-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046218244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2001.02536.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046371872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2001.5378", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050919613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi020355q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055195365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi020355q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055195365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-144-3-801", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060369800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-148-11-3539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060370938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/mic.0.27061-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060397062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1090827", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062449036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.176.6.1738-1745.1994", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062723536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.177.3.765-773.1995", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062724479"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077212099", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078296836", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-04", 
    "datePublishedReg": "2006-04-01", 
    "description": "YloQ, from Bacillus subtilis, was identified previously as an essential nucleotide-binding protein of unknown function. YloQ was successfully over-expressed in Escherichia coli in soluble form. The purified protein displayed a low GTPase activity similar to that of other small bacterial GTPases such as Bex/Era. Based on the demonstrated GTPase activity and the unusual order of the yloQ G motifs, we now designate this protein as CpgA (circularly permuted GTPase). An unexpected property of this low abundance GTPase was the demonstration, using gel filtration and ultracentrifugation analysis, that the protein formed stable dimers, dependent upon the concentration of YloQ(CpgA), but independent of GTP. In order to investigate function, cpgA was placed under the control of the pspac promotor in the B. subtilis chromosome. When grown in E or Spizizen medium in the absence of IPTG, the rate of growth was significantly reduced. A large proportion of the cells exhibited a markedly perturbed morphology, with the formation of swollen, bent or 'curly' shapes. To confirm that this was specifically due to depleted CpgA a plasmid-borne cpgA under pxyl control was introduced. This restored normal cell shape and growth rate, even in the absence of IPTG, provided xylose was present. The crystal structure of CpgA(YloQ) suggests a role as a translation initiation factor and we discuss the possibility that CpgA is involved in the translation of a subset of proteins, including some required for shape maintenance.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00438-006-0097-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "275"
      }
    ], 
    "name": "The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis", 
    "pagination": "409-420", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4d2e121ea4a0faa996e4255ae54cf4277de22742b7d4620a5a32cc51ec7b8074"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16485133"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101093320"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00438-006-0097-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1016197900"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00438-006-0097-9", 
      "https://app.dimensions.ai/details/publication/pub.1016197900"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:54", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000364_0000000364/records_72859_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00438-006-0097-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0097-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0097-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0097-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00438-006-0097-9'


 

This table displays all metadata directly associated to this object as RDF triples.

300 TRIPLES      21 PREDICATES      84 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00438-006-0097-9 schema:about N091a37813ea34775a58cd3ad7cc983f3
2 N1532c626e1994487a7dad7e692e92f1a
3 N37cac527e85c40f5b42674a02d52bca0
4 N437bf753c4ef4531b9d4362edffaf88e
5 N4f2a82bf7bd14abfbba8468c6f923e9f
6 N588dff03f5a9432e8d9f25cb1186071f
7 N61876236bdc746658549b345e28344d7
8 N67d092fd6adb41dcb49663b7be949a7b
9 N720286f60c964b4eb9f49d32b14714a7
10 N7eb5d523fa984a50b3ad420a1be89331
11 N913a4a9b4b6849e38eae27a3462e3a43
12 N978a7555d9234e2caa61b2ceb840cfb8
13 N98f81c061a57477fb7a0fa3e4c62ab17
14 Nb736cba5dce349908b3171337933a1db
15 Nba33484ef118433db71e3d8add29859f
16 Neb95238310af441d835d343c75661b99
17 Nf1a5620cfa6948e8bf1f7b47ec9ac3ed
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author N55f523318d8c449fa1603fdc81970234
21 schema:citation sg:pub.10.1007/s00203-005-0015-2
22 sg:pub.10.1038/227680a0
23 sg:pub.10.1038/36786
24 sg:pub.10.1038/nature02250
25 sg:pub.10.1038/nbt0396-315
26 sg:pub.10.1038/nbt0998-851
27 https://app.dimensions.ai/details/publication/pub.1077212099
28 https://app.dimensions.ai/details/publication/pub.1078296836
29 https://doi.org/10.1006/jmbi.2001.5378
30 https://doi.org/10.1016/0003-2697(79)90115-5
31 https://doi.org/10.1016/j.jmb.2003.09.079
32 https://doi.org/10.1016/j.jmb.2004.05.029
33 https://doi.org/10.1016/s0006-3495(00)76713-0
34 https://doi.org/10.1016/s0022-2836(03)00579-5
35 https://doi.org/10.1016/s0022-2836(83)80284-8
36 https://doi.org/10.1016/s1369-5274(03)00037-7
37 https://doi.org/10.1021/bi020355q
38 https://doi.org/10.1042/bj20041873
39 https://doi.org/10.1042/bj20050733
40 https://doi.org/10.1046/j.1365-2958.2001.02536.x
41 https://doi.org/10.1046/j.1365-2958.2002.03178.x
42 https://doi.org/10.1046/j.1365-2958.2003.03475.x
43 https://doi.org/10.1073/pnas.0405202101
44 https://doi.org/10.1073/pnas.0730515100
45 https://doi.org/10.1093/nar/gkh861
46 https://doi.org/10.1099/00221287-144-3-801
47 https://doi.org/10.1099/00221287-148-11-3539
48 https://doi.org/10.1099/mic.0.27061-0
49 https://doi.org/10.1111/j.1365-2958.2004.04050.x
50 https://doi.org/10.1111/j.1365-2958.2004.04343.x
51 https://doi.org/10.1126/science.1090827
52 https://doi.org/10.1128/aem.67.1.403-410.2001
53 https://doi.org/10.1128/jb.176.6.1738-1745.1994
54 https://doi.org/10.1128/jb.177.3.765-773.1995
55 https://doi.org/10.1128/jb.182.19.5634-5638.2000
56 https://doi.org/10.1128/jb.186.11.3660-3662.2004
57 https://doi.org/10.1128/jb.186.5.1381-1387.2004
58 https://doi.org/10.1146/annurev.biochem.66.1.639
59 schema:datePublished 2006-04
60 schema:datePublishedReg 2006-04-01
61 schema:description YloQ, from Bacillus subtilis, was identified previously as an essential nucleotide-binding protein of unknown function. YloQ was successfully over-expressed in Escherichia coli in soluble form. The purified protein displayed a low GTPase activity similar to that of other small bacterial GTPases such as Bex/Era. Based on the demonstrated GTPase activity and the unusual order of the yloQ G motifs, we now designate this protein as CpgA (circularly permuted GTPase). An unexpected property of this low abundance GTPase was the demonstration, using gel filtration and ultracentrifugation analysis, that the protein formed stable dimers, dependent upon the concentration of YloQ(CpgA), but independent of GTP. In order to investigate function, cpgA was placed under the control of the pspac promotor in the B. subtilis chromosome. When grown in E or Spizizen medium in the absence of IPTG, the rate of growth was significantly reduced. A large proportion of the cells exhibited a markedly perturbed morphology, with the formation of swollen, bent or 'curly' shapes. To confirm that this was specifically due to depleted CpgA a plasmid-borne cpgA under pxyl control was introduced. This restored normal cell shape and growth rate, even in the absence of IPTG, provided xylose was present. The crystal structure of CpgA(YloQ) suggests a role as a translation initiation factor and we discuss the possibility that CpgA is involved in the translation of a subset of proteins, including some required for shape maintenance.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree false
65 schema:isPartOf Nbe28b8b8b32d4693a8c53bf9401415c8
66 Nd53048568bc2493fb6b264b28a050989
67 sg:journal.1297380
68 schema:name The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis
69 schema:pagination 409-420
70 schema:productId N130cd9fde8ca4e03a4f492882a73b26e
71 N4d20d56d61fc4eacb05cd84a7efb67f6
72 Nb0e1515709424e26a7183417d8cbf2b6
73 Nfb25a506c96044bf995068e109bc06bc
74 Nffc68954407f40aebfb8c21f21328275
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016197900
76 https://doi.org/10.1007/s00438-006-0097-9
77 schema:sdDatePublished 2019-04-11T12:54
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher N00737af059104e7ba599a4c376de6f1a
80 schema:url http://link.springer.com/10.1007%2Fs00438-006-0097-9
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N00737af059104e7ba599a4c376de6f1a schema:name Springer Nature - SN SciGraph project
85 rdf:type schema:Organization
86 N046ea7d9d92d425daa57e958deb51e5a rdf:first sg:person.0601464400.93
87 rdf:rest N53a9ebe705994cea8df68b587aab9270
88 N091a37813ea34775a58cd3ad7cc983f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Multigene Family
90 rdf:type schema:DefinedTerm
91 N130cd9fde8ca4e03a4f492882a73b26e schema:name pubmed_id
92 schema:value 16485133
93 rdf:type schema:PropertyValue
94 N1532c626e1994487a7dad7e692e92f1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Base Sequence
96 rdf:type schema:DefinedTerm
97 N37cac527e85c40f5b42674a02d52bca0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Cell Wall
99 rdf:type schema:DefinedTerm
100 N437bf753c4ef4531b9d4362edffaf88e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Dimerization
102 rdf:type schema:DefinedTerm
103 N4d20d56d61fc4eacb05cd84a7efb67f6 schema:name doi
104 schema:value 10.1007/s00438-006-0097-9
105 rdf:type schema:PropertyValue
106 N4f2a82bf7bd14abfbba8468c6f923e9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Amino Acid Sequence
108 rdf:type schema:DefinedTerm
109 N53a9ebe705994cea8df68b587aab9270 rdf:first sg:person.01135131247.31
110 rdf:rest N53ae8d6b6312452aa42b8c1f9d8a81d6
111 N53ae8d6b6312452aa42b8c1f9d8a81d6 rdf:first sg:person.0724310230.58
112 rdf:rest Ndce396f6bd174744bae755c249f34217
113 N55f523318d8c449fa1603fdc81970234 rdf:first sg:person.01021617122.62
114 rdf:rest Nccfdc13f8f724e2ab85d77d3d8a47613
115 N588dff03f5a9432e8d9f25cb1186071f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Amino Acid Motifs
117 rdf:type schema:DefinedTerm
118 N61876236bdc746658549b345e28344d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Gene Expression Regulation, Bacterial
120 rdf:type schema:DefinedTerm
121 N67d092fd6adb41dcb49663b7be949a7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Guanosine Triphosphate
123 rdf:type schema:DefinedTerm
124 N720286f60c964b4eb9f49d32b14714a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Bacterial Proteins
126 rdf:type schema:DefinedTerm
127 N7eb5d523fa984a50b3ad420a1be89331 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Molecular Sequence Data
129 rdf:type schema:DefinedTerm
130 N913a4a9b4b6849e38eae27a3462e3a43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Bacillus subtilis
132 rdf:type schema:DefinedTerm
133 N978a7555d9234e2caa61b2ceb840cfb8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Prokaryotic Initiation Factors
135 rdf:type schema:DefinedTerm
136 N98f81c061a57477fb7a0fa3e4c62ab17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Conserved Sequence
138 rdf:type schema:DefinedTerm
139 Nb0e1515709424e26a7183417d8cbf2b6 schema:name readcube_id
140 schema:value 4d2e121ea4a0faa996e4255ae54cf4277de22742b7d4620a5a32cc51ec7b8074
141 rdf:type schema:PropertyValue
142 Nb736cba5dce349908b3171337933a1db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Morphogenesis
144 rdf:type schema:DefinedTerm
145 Nba33484ef118433db71e3d8add29859f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Phosphoprotein Phosphatases
147 rdf:type schema:DefinedTerm
148 Nbe28b8b8b32d4693a8c53bf9401415c8 schema:issueNumber 4
149 rdf:type schema:PublicationIssue
150 Nccfdc13f8f724e2ab85d77d3d8a47613 rdf:first sg:person.0646102556.44
151 rdf:rest Nfad589eb549b4158a666f667543c282b
152 Nd53048568bc2493fb6b264b28a050989 schema:volumeNumber 275
153 rdf:type schema:PublicationVolume
154 Ndce396f6bd174744bae755c249f34217 rdf:first sg:person.01227030231.00
155 rdf:rest rdf:nil
156 Neb95238310af441d835d343c75661b99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Isopropyl Thiogalactoside
158 rdf:type schema:DefinedTerm
159 Nf1a5620cfa6948e8bf1f7b47ec9ac3ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name GTP Phosphohydrolases
161 rdf:type schema:DefinedTerm
162 Nfad589eb549b4158a666f667543c282b rdf:first sg:person.01352772464.79
163 rdf:rest N046ea7d9d92d425daa57e958deb51e5a
164 Nfb25a506c96044bf995068e109bc06bc schema:name dimensions_id
165 schema:value pub.1016197900
166 rdf:type schema:PropertyValue
167 Nffc68954407f40aebfb8c21f21328275 schema:name nlm_unique_id
168 schema:value 101093320
169 rdf:type schema:PropertyValue
170 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
171 schema:name Biological Sciences
172 rdf:type schema:DefinedTerm
173 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
174 schema:name Biochemistry and Cell Biology
175 rdf:type schema:DefinedTerm
176 sg:journal.1297380 schema:issn 1432-1874
177 1617-4615
178 schema:name Molecular Genetics and Genomics
179 rdf:type schema:Periodical
180 sg:person.01021617122.62 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
181 schema:familyName Cladière
182 schema:givenName Lionel
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021617122.62
184 rdf:type schema:Person
185 sg:person.01135131247.31 schema:affiliation https://www.grid.ac/institutes/grid.5685.e
186 schema:familyName Wilkinson
187 schema:givenName Anthony J.
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135131247.31
189 rdf:type schema:Person
190 sg:person.01227030231.00 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
191 schema:familyName Séror
192 schema:givenName Simone J.
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227030231.00
194 rdf:type schema:Person
195 sg:person.01352772464.79 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
196 schema:familyName Madec
197 schema:givenName Edwige
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352772464.79
199 rdf:type schema:Person
200 sg:person.0601464400.93 schema:affiliation https://www.grid.ac/institutes/grid.5685.e
201 schema:familyName Levdikov
202 schema:givenName Vladimir M.
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601464400.93
204 rdf:type schema:Person
205 sg:person.0646102556.44 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
206 schema:familyName Hamze
207 schema:givenName Kassem
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646102556.44
209 rdf:type schema:Person
210 sg:person.0724310230.58 schema:affiliation https://www.grid.ac/institutes/grid.462411.4
211 schema:familyName Holland
212 schema:givenName I. Barry
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724310230.58
214 rdf:type schema:Person
215 sg:pub.10.1007/s00203-005-0015-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013782862
216 https://doi.org/10.1007/s00203-005-0015-2
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
219 https://doi.org/10.1038/227680a0
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/36786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031054310
222 https://doi.org/10.1038/36786
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nature02250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002922834
225 https://doi.org/10.1038/nature02250
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nbt0396-315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007123993
228 https://doi.org/10.1038/nbt0396-315
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/nbt0998-851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027411938
231 https://doi.org/10.1038/nbt0998-851
232 rdf:type schema:CreativeWork
233 https://app.dimensions.ai/details/publication/pub.1077212099 schema:CreativeWork
234 https://app.dimensions.ai/details/publication/pub.1078296836 schema:CreativeWork
235 https://doi.org/10.1006/jmbi.2001.5378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050919613
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/0003-2697(79)90115-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016788675
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/j.jmb.2003.09.079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042341661
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.jmb.2004.05.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012856629
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0006-3495(00)76713-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014356043
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/s0022-2836(03)00579-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010893831
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/s0022-2836(83)80284-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046218244
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/s1369-5274(03)00037-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006496719
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1021/bi020355q schema:sameAs https://app.dimensions.ai/details/publication/pub.1055195365
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1042/bj20041873 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046123029
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1042/bj20050733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033214675
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1046/j.1365-2958.2001.02536.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046371872
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1046/j.1365-2958.2002.03178.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041486871
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1046/j.1365-2958.2003.03475.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024348638
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1073/pnas.0405202101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046123730
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1073/pnas.0730515100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033908509
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1093/nar/gkh861 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029836522
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1099/00221287-144-3-801 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060369800
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1099/00221287-148-11-3539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060370938
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1099/mic.0.27061-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060397062
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1111/j.1365-2958.2004.04050.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037148569
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1111/j.1365-2958.2004.04343.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034744741
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1126/science.1090827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062449036
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1128/aem.67.1.403-410.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032274862
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1128/jb.176.6.1738-1745.1994 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062723536
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1128/jb.177.3.765-773.1995 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062724479
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1128/jb.182.19.5634-5638.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004185665
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1128/jb.186.11.3660-3662.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009174074
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1128/jb.186.5.1381-1387.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034149426
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1146/annurev.biochem.66.1.639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044366676
294 rdf:type schema:CreativeWork
295 https://www.grid.ac/institutes/grid.462411.4 schema:alternateName Institute of Integrative Biology of the Cell
296 schema:name Institut de Génétique et Microbiologie, Université Paris-Sud, UMR CNRS 8621, Bâtiment 409, 91405, Orsay Cedex, France
297 rdf:type schema:Organization
298 https://www.grid.ac/institutes/grid.5685.e schema:alternateName University of York
299 schema:name Structural Biology Laboratory, Department of Chemistry, University of York, YO10, York, SYW, UK
300 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...