Concomitant reiterative BAC walking and fine genetic mapping enable physical map development for the broad-spectrum late blight resistance region, RB View Full Text


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Article Info

DATE

2003-08

AUTHORS

J. M. Bradeen, S. K. Naess, J. Song, G. T. Haberlach, S. M. Wielgus, C. R. Buell, J. Jiang, J. P. Helgeson

ABSTRACT

The wild potato species Solanum bulbocastanum is a source of genes for potent late blight resistance. We previously mapped resistance to a single region of the S. bulbocastanum chromosome 8 and named the region RB (for "Resistance from S. Bulbocastanum"). We now report physical mapping and contig construction for the RB region via a novel reiterative method of BAC walking and concomitant fine genetic mapping. BAC walking was initiated using RFLP markers previously shown to be associated with late blight resistance. Subcontig extension was accomplished using new probes developed from BAC ends. Significantly, BAC end and partial BAC sequences were also used to develop PCR-based markers to enhance map resolution in the RB region. As they were developed from BAC clones of known position relative to RB, our PCR-based markers are known a priori to be physically closer to the resistance region. These markers allowed the efficient screening of large numbers of segregating progeny at the cotyledon stage, and permitted us to assign the resistance phenotype to a region of approximately 55 kb. Our markers also directed BAC walking efforts away from regions distantly related to RB in favor of the 55-kb region. Because the S. bulbocastanum genotype used in BAC library construction is heterozygous for RB (RB/rb), codominant PCR-based markers, originally developed for fine-scale mapping, were also used to determine homolog origins for individual BAC clones. Ultimately, BAC contigs were constructed for the RB region from both resistant (RB) and susceptible (rb) homologs. More... »

PAGES

603-611

References to SciGraph publications

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  • 2001-08. Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum in MOLECULAR GENETICS AND GENOMICS
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  • 1995-01. A high-resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markers in MOLECULAR GENETICS AND GENOMICS
  • 1996-04. RFLP analysis of resistance to Columbia root-knot nematode derived from Solanum bulbocastanum in a BC2 population in THEORETICAL AND APPLIED GENETICS
  • 2000-10. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8 in THEORETICAL AND APPLIED GENETICS
  • 2002-10. Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome in MOLECULAR GENETICS AND GENOMICS
  • 2002-02. Targeted isolation, sequence analysis, and physical mapping of nonTIR NBS-LRR genes in soybean in THEORETICAL AND APPLIED GENETICS
  • 1996-12. A PCR–based approach for isolating pathogen resistance genes from potato with potential for wide application in plants in NATURE GENETICS
  • 1994-09. Recombination of Solanum brevidens chromosomes in the second backcross generation from a somatic hybrid with S. tuberosum in THEORETICAL AND APPLIED GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00438-003-0865-8

    DOI

    http://dx.doi.org/10.1007/s00438-003-0865-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027891760

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12827499


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