Two short regions of the promoter are essential for activation and repression of the nitrate reductase gene in Chlamydomonas reinhardtii View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-09

AUTHORS

R. Loppes, M. Radoux

ABSTRACT

In Chlamydomonas reinhardtii, the expression of the Nia1 gene encoding NAD(P)H nitrate reductase is controlled at the transcriptional level, positively by light and nitrate and negatively by ammonium. The sequences lying between positions -247 and -25 with respect to the start site of transcription were analyzed for the presence of regulatory elements using an arylsulfatase reporter gene ( Ars) fused to a minimal beta-tubulin promoter. An 84-bp sequence resulting from the joining of two partially homologous regions (-231 to -201 and -77 to -25) was shown to be necessary and sufficient to ensure activation and repression of the reporter gene. Interestingly, this shortened construct overexpressed the Ars gene in cells grown in nitrate-containing medium, relative to the construct bearing the complete -247 to -25 sequence. The 223-bp sequence was subjected to linker-scan analyses in the two regions of interest (-231 to -201 and -77 to -25). Most mutations introduced into this 84-bp sequence were shown to affect transcriptional activation on nitrate. Many of them also resulted in significantly increased arylsulfatase levels in cultures grown on ammonium. We therefore propose that the two regions act as bifunctional elements, stimulating or inhibiting the activity of the Nia1 promoter depending on the nature of the nitrogen source. More... »

PAGES

42-48

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00438-002-0719-9

DOI

http://dx.doi.org/10.1007/s00438-002-0719-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043592006

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12242497


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chlamydomonas reinhardtii", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Enzymologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitrate Reductase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitrate Reductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Promoter Regions, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Li\u00e8ge", 
          "id": "https://www.grid.ac/institutes/grid.4861.b", 
          "name": [
            "Genetics of Microorganisms, Department of Plant Biology,B22, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Loppes", 
        "givenName": "R.", 
        "id": "sg:person.0100713403.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0100713403.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Li\u00e8ge", 
          "id": "https://www.grid.ac/institutes/grid.4861.b", 
          "name": [
            "Genetics of Microorganisms, Department of Plant Biology,B22, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Radoux", 
        "givenName": "M.", 
        "id": "sg:person.0727356244.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727356244.52"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2002-09", 
    "datePublishedReg": "2002-09-01", 
    "description": "In Chlamydomonas reinhardtii, the expression of the Nia1 gene encoding NAD(P)H nitrate reductase is controlled at the transcriptional level, positively by light and nitrate and negatively by ammonium. The sequences lying between positions -247 and -25 with respect to the start site of transcription were analyzed for the presence of regulatory elements using an arylsulfatase reporter gene ( Ars) fused to a minimal beta-tubulin promoter. An 84-bp sequence resulting from the joining of two partially homologous regions (-231 to -201 and -77 to -25) was shown to be necessary and sufficient to ensure activation and repression of the reporter gene. Interestingly, this shortened construct overexpressed the Ars gene in cells grown in nitrate-containing medium, relative to the construct bearing the complete -247 to -25 sequence. The 223-bp sequence was subjected to linker-scan analyses in the two regions of interest (-231 to -201 and -77 to -25). Most mutations introduced into this 84-bp sequence were shown to affect transcriptional activation on nitrate. Many of them also resulted in significantly increased arylsulfatase levels in cultures grown on ammonium. We therefore propose that the two regions act as bifunctional elements, stimulating or inhibiting the activity of the Nia1 promoter depending on the nature of the nitrogen source.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00438-002-0719-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "268"
      }
    ], 
    "name": "Two short regions of the promoter are essential for activation and repression of the nitrate reductase gene in Chlamydomonas reinhardtii", 
    "pagination": "42-48", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "cfa4b58ecaad4411b029f1a1a60eae1d5b43baa77170945cd24830d03f5ac23c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12242497"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101093320"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00438-002-0719-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1043592006"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00438-002-0719-9", 
      "https://app.dimensions.ai/details/publication/pub.1043592006"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:09", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00438-002-0719-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00438-002-0719-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00438-002-0719-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00438-002-0719-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00438-002-0719-9'


 

This table displays all metadata directly associated to this object as RDF triples.

124 TRIPLES      20 PREDICATES      41 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00438-002-0719-9 schema:about N30c68efe0bb84c9bb46bba3e054f8111
2 N590d9aab3b3b4136b98dc28cab372e1f
3 N6a959a46ab9c40898f0cf4a83b82c935
4 Na1305a8e2c2b4b36a7e1309bf8152de1
5 Na14abbd9d6e3468e8c8b5a891da58035
6 Na8150dbcbd604bc2bff44d325bfb3d1d
7 Nc293877ea63b49bea551a3ecfed19b51
8 Nc4526321422d46afbf2b60792398d3e6
9 Nf01b6e7455d7488899f44ce79994f33a
10 Nf0493f5fb8d2483cb61453352b5cef35
11 Nf6252e5b692c4f48b4e0832ef482b01c
12 Nf7a341f1cf574b45b9ad2623d6380cd0
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author N8a25b9c79770433785978e0177c5d9be
16 schema:datePublished 2002-09
17 schema:datePublishedReg 2002-09-01
18 schema:description In Chlamydomonas reinhardtii, the expression of the Nia1 gene encoding NAD(P)H nitrate reductase is controlled at the transcriptional level, positively by light and nitrate and negatively by ammonium. The sequences lying between positions -247 and -25 with respect to the start site of transcription were analyzed for the presence of regulatory elements using an arylsulfatase reporter gene ( Ars) fused to a minimal beta-tubulin promoter. An 84-bp sequence resulting from the joining of two partially homologous regions (-231 to -201 and -77 to -25) was shown to be necessary and sufficient to ensure activation and repression of the reporter gene. Interestingly, this shortened construct overexpressed the Ars gene in cells grown in nitrate-containing medium, relative to the construct bearing the complete -247 to -25 sequence. The 223-bp sequence was subjected to linker-scan analyses in the two regions of interest (-231 to -201 and -77 to -25). Most mutations introduced into this 84-bp sequence were shown to affect transcriptional activation on nitrate. Many of them also resulted in significantly increased arylsulfatase levels in cultures grown on ammonium. We therefore propose that the two regions act as bifunctional elements, stimulating or inhibiting the activity of the Nia1 promoter depending on the nature of the nitrogen source.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N6ac4f1658fd54ece82b984d821934c06
23 Nd22a6b3563784606b7711320c3fcaafc
24 sg:journal.1297380
25 schema:name Two short regions of the promoter are essential for activation and repression of the nitrate reductase gene in Chlamydomonas reinhardtii
26 schema:pagination 42-48
27 schema:productId N0aa1b8dd7f344ac292ea0d17acab1afb
28 N26f7a1f8f34c476fa8a024745ede846b
29 N40ea228889374975beb5d26abfee8eca
30 N64523fc9157146baaa3b2185fc59fad8
31 Nb6ecb7c75f164990bfc27cfa932063a3
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043592006
33 https://doi.org/10.1007/s00438-002-0719-9
34 schema:sdDatePublished 2019-04-10T19:09
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher N512bf82ed4f74eedb518ef75139d8daf
37 schema:url http://link.springer.com/10.1007%2Fs00438-002-0719-9
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N0aa1b8dd7f344ac292ea0d17acab1afb schema:name dimensions_id
42 schema:value pub.1043592006
43 rdf:type schema:PropertyValue
44 N26f7a1f8f34c476fa8a024745ede846b schema:name nlm_unique_id
45 schema:value 101093320
46 rdf:type schema:PropertyValue
47 N30c68efe0bb84c9bb46bba3e054f8111 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Nitrate Reductases
49 rdf:type schema:DefinedTerm
50 N40ea228889374975beb5d26abfee8eca schema:name doi
51 schema:value 10.1007/s00438-002-0719-9
52 rdf:type schema:PropertyValue
53 N512bf82ed4f74eedb518ef75139d8daf schema:name Springer Nature - SN SciGraph project
54 rdf:type schema:Organization
55 N590d9aab3b3b4136b98dc28cab372e1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
56 schema:name Sequence Deletion
57 rdf:type schema:DefinedTerm
58 N64523fc9157146baaa3b2185fc59fad8 schema:name pubmed_id
59 schema:value 12242497
60 rdf:type schema:PropertyValue
61 N6a959a46ab9c40898f0cf4a83b82c935 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Nitrate Reductase
63 rdf:type schema:DefinedTerm
64 N6ac4f1658fd54ece82b984d821934c06 schema:issueNumber 1
65 rdf:type schema:PublicationIssue
66 N6f77e1fd5a9b4d62b79f36bf7c12d547 rdf:first sg:person.0727356244.52
67 rdf:rest rdf:nil
68 N8a25b9c79770433785978e0177c5d9be rdf:first sg:person.0100713403.31
69 rdf:rest N6f77e1fd5a9b4d62b79f36bf7c12d547
70 Na1305a8e2c2b4b36a7e1309bf8152de1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Gene Expression Regulation, Enzymologic
72 rdf:type schema:DefinedTerm
73 Na14abbd9d6e3468e8c8b5a891da58035 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Promoter Regions, Genetic
75 rdf:type schema:DefinedTerm
76 Na8150dbcbd604bc2bff44d325bfb3d1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name RNA, Messenger
78 rdf:type schema:DefinedTerm
79 Nb6ecb7c75f164990bfc27cfa932063a3 schema:name readcube_id
80 schema:value cfa4b58ecaad4411b029f1a1a60eae1d5b43baa77170945cd24830d03f5ac23c
81 rdf:type schema:PropertyValue
82 Nc293877ea63b49bea551a3ecfed19b51 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Animals
84 rdf:type schema:DefinedTerm
85 Nc4526321422d46afbf2b60792398d3e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Genes, Reporter
87 rdf:type schema:DefinedTerm
88 Nd22a6b3563784606b7711320c3fcaafc schema:volumeNumber 268
89 rdf:type schema:PublicationVolume
90 Nf01b6e7455d7488899f44ce79994f33a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Molecular Sequence Data
92 rdf:type schema:DefinedTerm
93 Nf0493f5fb8d2483cb61453352b5cef35 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Transcription, Genetic
95 rdf:type schema:DefinedTerm
96 Nf6252e5b692c4f48b4e0832ef482b01c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Chlamydomonas reinhardtii
98 rdf:type schema:DefinedTerm
99 Nf7a341f1cf574b45b9ad2623d6380cd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Base Sequence
101 rdf:type schema:DefinedTerm
102 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
103 schema:name Biological Sciences
104 rdf:type schema:DefinedTerm
105 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
106 schema:name Genetics
107 rdf:type schema:DefinedTerm
108 sg:journal.1297380 schema:issn 1432-1874
109 1617-4615
110 schema:name Molecular Genetics and Genomics
111 rdf:type schema:Periodical
112 sg:person.0100713403.31 schema:affiliation https://www.grid.ac/institutes/grid.4861.b
113 schema:familyName Loppes
114 schema:givenName R.
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0100713403.31
116 rdf:type schema:Person
117 sg:person.0727356244.52 schema:affiliation https://www.grid.ac/institutes/grid.4861.b
118 schema:familyName Radoux
119 schema:givenName M.
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727356244.52
121 rdf:type schema:Person
122 https://www.grid.ac/institutes/grid.4861.b schema:alternateName University of Liège
123 schema:name Genetics of Microorganisms, Department of Plant Biology,B22, University of Liège, 4000 Liège, Belgium
124 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...