Cloning and sequence characterisation of falcipain-2 from Plasmodium falciparum Gombak A strain (Malaysia) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-09

AUTHORS

Tiow-Suan Sim, Paxton Loke, May Lee, Mulkit Singh, Horst Flotow

ABSTRACT

In this study, the genome of the Plasmodium falciparum Gombak A strain was examined for the presence of a gene encoding falcipain-2, a cysteine protease, using homology-based polymerase chain reaction cloning. The nucleotide sequence obtained from the gene cloned (designated pFG1) is approximately 99% homologous to other falcipain-2 genes from different strains. Comparatively, it is 69% homologous to falcipain-3 genes. Direct cloning of the falcipain-2 gene and its resemblance to the reported corresponding mRNA transcript suggests the absence of introns in this gene. Sequence alignment and comparison revealed four amino acid differences at positions 15, 51, 59 and 414 in the falcipain-2 from P. falciparum Gombak A as compared to other falcipain-2 proteins from different strains. More... »

PAGES

683-686

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004360100444

DOI

http://dx.doi.org/10.1007/s004360100444

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008820167

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11570549


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