Evaluation of immunomagnetic separation method for detection of Giardia for different reaction times and reaction volumes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-06

AUTHORS

Bing-Mu Hsu, Chihpin Huang, Yu-Che Lai, Hua-Shan Tai, Ying-Chien Chung

ABSTRACT

Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia. In this study, Dynal IMS was evaluated on the basis of recovery efficiencies of Giardia cysts for various IMS operational conditions. The average recoveries for Giardia in deionized, treated and raw water samples were 82.6 +/- 12.2% (n = 6), 75.6 +/- 15.2% (n = 3), and 70.6 +/- 18.2% (n = 3), respectively. Significant changes in recovery were observed by altering the debris ratio and the debris components of water samples. Changing the reaction volume within the same vessel had no significant effect on cyst recovery efficiencies. However, prolonging the reaction time did increase recovery efficiencies. More... »

PAGES

472-474

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004360100377

DOI

http://dx.doi.org/10.1007/s004360100377

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008301138

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11411947


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