Ontology type: schema:ScholarlyArticle
2021-11-26
AUTHORSHaneul Jung, Jeong Yeon Won, Yun-Kyu Park, Jin-Hee Han, Seok Ho Cha
ABSTRACTClonorchis sinensis (Cs) is a common trematode in Asian countries. Infection by Cs can result in many clinical symptoms. Here, a cDNA encoding a Cs apical sodium-dependent bile acid transporter (CsSBAT) was isolated from a Cs cDNA library, and functional characterization was performed using Xenopus laevis oocyte expression system. When expressed in Xenopus laevis oocytes, CsSBAT mediated the transport of radiolabeled estrone sulfate and dehydroepiandrosterone sulfate. No trans-uptake of carnitine, estradiol 17 β-D glucuronide, prostaglandin E2, p-aminohippuric acid, α-ketoglutaric acid, and tetraethylammonium was observed. CsSBAT-mediated estrone sulfate uptake was in a time- and sodium-dependent manner. CsSBAT showed no exchange properties in efflux experiments. Concentration-dependent results showed saturable kinetics consistent with the Michaelis–Menten equation. Nonlinear regression analyses yielded a Km value of 0.3 ± 0.04 μM for [3H]estrone sulfate. CsSBAT-mediated estrone sulfate uptake was strongly inhibited by sulfate conjugates but not glucuronide conjugates. These findings contribute to our understanding of CsSBAT transport properties and the cascade of estrogen metabolite movement in Cs. More... »
PAGES217-224
http://scigraph.springernature.com/pub.10.1007/s00436-021-07393-4
DOIhttp://dx.doi.org/10.1007/s00436-021-07393-4
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1143200805
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/34825261
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1116",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Physiology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Bile Acids and Salts",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Clonorchis sinensis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Oocytes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sodium",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sulfate Transporters",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Symporters",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea",
"id": "http://www.grid.ac/institutes/grid.202119.9",
"name": [
"Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Jung",
"givenName": "Haneul",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea",
"id": "http://www.grid.ac/institutes/grid.202119.9",
"name": [
"Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Won",
"givenName": "Jeong Yeon",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea",
"id": "http://www.grid.ac/institutes/grid.202119.9",
"name": [
"Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Park",
"givenName": "Yun-Kyu",
"id": "sg:person.015702613724.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015702613724.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do, Republic of Korea",
"id": "http://www.grid.ac/institutes/grid.412010.6",
"name": [
"Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Han",
"givenName": "Jin-Hee",
"id": "sg:person.0704165056.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704165056.08"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea",
"id": "http://www.grid.ac/institutes/grid.202119.9",
"name": [
"Department of Parasitology and Tropical Medicine, Inha University School of Medicine, 400-712, Incheon, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Cha",
"givenName": "Seok Ho",
"id": "sg:person.01253747763.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253747763.19"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1186/s40249-016-0166-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019415164",
"https://doi.org/10.1186/s40249-016-0166-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00436-006-0441-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031104507",
"https://doi.org/10.1007/s00436-006-0441-3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00436-007-0778-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012930351",
"https://doi.org/10.1007/s00436-007-0778-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00436-010-1936-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040763824",
"https://doi.org/10.1007/s00436-010-1936-5"
],
"type": "CreativeWork"
}
],
"datePublished": "2021-11-26",
"datePublishedReg": "2021-11-26",
"description": "Clonorchis sinensis (Cs) is a common trematode in Asian countries. Infection by Cs can result in many clinical symptoms. Here, a cDNA encoding a Cs apical sodium-dependent bile acid transporter (CsSBAT) was isolated from a Cs cDNA library, and functional characterization was performed using Xenopus laevis oocyte expression system. When expressed in Xenopus laevis oocytes, CsSBAT mediated the transport of radiolabeled estrone sulfate and dehydroepiandrosterone sulfate. No trans-uptake of carnitine, estradiol 17 \u03b2-D glucuronide, prostaglandin E2, p-aminohippuric acid, \u03b1-ketoglutaric acid, and tetraethylammonium was observed. CsSBAT-mediated estrone sulfate uptake was in a time- and sodium-dependent manner. CsSBAT showed no exchange properties in efflux experiments. Concentration-dependent results showed saturable kinetics consistent with the Michaelis\u2013Menten equation. Nonlinear regression analyses yielded a Km value of 0.3\u2009\u00b1\u20090.04\u00a0\u03bcM for [3H]estrone sulfate. CsSBAT-mediated estrone sulfate uptake was strongly inhibited by sulfate conjugates but not glucuronide conjugates. These findings contribute to our understanding of CsSBAT transport properties and the cascade of estrogen metabolite movement in Cs.",
"genre": "article",
"id": "sg:pub.10.1007/s00436-021-07393-4",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1096949",
"issn": [
"0932-0113",
"1432-1955"
],
"name": "Parasitology Research",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "121"
}
],
"keywords": [
"estrone sulfate uptake",
"Xenopus laevis oocyte expression system",
"apical sodium-dependent bile acid transporter",
"sodium-dependent bile acid transporter",
"bile acid transporter",
"clinical symptoms",
"sodium-dependent manner",
"prostaglandin E2",
"estrone sulfate",
"glucuronide conjugates",
"oocyte expression system",
"sulfate conjugates",
"aminohippuric acid",
"Xenopus laevis oocytes",
"regression analysis",
"efflux experiments",
"acid transporters",
"saturable kinetics",
"laevis oocytes",
"functional characterization",
"D-glucuronide",
"common trematode",
"sulfate uptake",
"symptoms",
"infection",
"transporters",
"conjugates",
"glucuronide",
"uptake",
"carnitine",
"tetraethylammonium",
"E2",
"Asian countries",
"oocytes",
"acid",
"expression system",
"findings",
"ketoglutaric acid",
"sulfate",
"cDNA library",
"cascade",
"trematodes",
"CS",
"\u03bcM",
"manner",
"Km values",
"cDNA",
"sinensis",
"nonlinear regression analysis",
"time",
"countries",
"Michaelis-Menten equation",
"concentration-dependent results",
"analysis",
"movement",
"results",
"understanding",
"metabolite movement",
"characterization",
"values",
"sulfate transporters",
"library",
"transport",
"system",
"kinetics",
"experiments",
"properties",
"exchange properties",
"equations",
"transport properties"
],
"name": "Functional characterization of Clonorchis sinensis sodium-bile acid co-transporter (CsSBAT) as a steroid sulfate transporter",
"pagination": "217-224",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1143200805"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00436-021-07393-4"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"34825261"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00436-021-07393-4",
"https://app.dimensions.ai/details/publication/pub.1143200805"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:39",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_911.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00436-021-07393-4"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00436-021-07393-4'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00436-021-07393-4'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00436-021-07393-4'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00436-021-07393-4'
This table displays all metadata directly associated to this object as RDF triples.
205 TRIPLES
22 PREDICATES
107 URIs
95 LITERALS
14 BLANK NODES