Evaluation of immunoprotective effects of recombinant proteins and DNA vaccines derived from Eimeria tenella surface antigen 6 and 15 in ... View Full Text


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Article Info

DATE

2021-11-24

AUTHORS

Tiantian Geng, Liyan Luo, Yale Wang, Bang Shen, Rui Fang, Min Hu, Junlong Zhao, Yanqin Zhou

ABSTRACT

Coccidiosis is an intestinal parasitic disease that causes huge economic losses to the poultry industry globally. Eimeria tenella belonging to protozoon is the causative agent of cecal coccidiosis in chicken, and it causes enormous damage to poultry industry. The surface antigens (SAGs) of apicomplexan parasites have functions of attachment and invasion in host-parasite interaction. As a result of parasitic invasion, host immune response is triggered. However, the immunogenicity and potency of E. tenella surface antigen 6 and 15 (EtSAG 6 and 15), as vaccinal candidate antigen, remain largely unknown. Therefore, gene fragments of E. tenella EtSAG 6 and 15 were amplified and transformed to pET28a prokaryotic vector for recombinant protein expression. The pEGFP-N1 eukaryotic vectors with EtSAG 6 and 15 amplification fragments (pEGFP-N1-EtSAG 5 and 16) were transformed into 293 T cell line. The results of reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis revealed successful expressions of EtSAG 6 and 15 in Escherichia coli and 293 T cells. Subsequently, animal experiments of 49 cobb broilers were performed to evaluate immunoprotection of recombinant proteins and DNA vaccines derived from E. tenella EtSAG 5 and 16 with an immunizing dose of 100 μg, respectively. Chickens vaccinated with rEtSAG 6 protein, rEtSAG 15 protein, pEGFP-N1-EtSAG 6 plasmid, or pEGFP-N1-EtSAG 15 plasmid showed no significant increase in IFN-γor interleukin-4 (IL-4) level compared with control groups. Chickens vaccinated with protein rEtSAG 6, protein rEtSAG 15, pEGFP-N1-EtSAG 6 plasmid, or pEGFP-N1-EtSAG 15 exhibited higher weight gains, lower oocyst output, and lower mean lesion scores, compared with infection control group. Among the four immunized groups, plasmid EGFP-N1-EtSAG 6 (100 μg) group exhibited the highest anticoccidial index (ACI) value (150.20). Overall, plasmids EGFP-N1-EtSAG 6 and 15, as DNA vaccines, provided a more effective immunoprotection for chickens against E. tenella than protein rEtSAG 6 and protein rEtSAG 15 as subunit vaccines. EtSAG 6 and 15 are promising candidate antigen genes for developing coccidiosis vaccine. More... »

PAGES

235-243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00436-021-07364-9

DOI

http://dx.doi.org/10.1007/s00436-021-07364-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1142911318

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/34816300


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106 parasitic diseases
107 parasitic invasion
108 plasmid
109 potency
110 poultry industry
111 prokaryotic vector
112 protein
113 protein expression
114 reaction
115 recombinant protein expression
116 recombinant proteins
117 response
118 results
119 reverse transcription-polymerase chain reaction
120 scores
121 significant increase
122 subunit vaccine
123 successful expression
124 surface
125 tenella
126 transcription-polymerase chain reaction
127 vaccine
128 values
129 vector
130 vivo
131 weight gain
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