Application of TaqMan qPCR for the detection and monitoring of Naegleria species in reservoirs used as a source for drinking ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10

AUTHORS

Po-Min Kao, Bing-Mu Hsu, Tsui-Kang Hsu, Yi-Chou Chiu, Chung-Liang Chang, Wen-Tsai Ji, Shih-Wei Huang, Cheng-Wei Fan

ABSTRACT

Naegleria spp. can be found in the natural aquatic environments. Naegleria fowleri can cause fatal infections in the central nervous system in humans and animals, and the most important source of infection is through direct water contact. In this study, PCR of 5.8S ribosomal RNA (rRNA) gene and internal transcribed spacer (ITS) region was performed in order to identify Naegleria isolates and quantify the Naegleria spp. by TaqMan real-time quantitative PCR in reservoir water samples. The occurrence of Naegleria spp. was investigated in 57 water samples from reservoirs with culture and PCR positive in 2 of them (3.5%), respectively. The total detection rate was 7.0% (4/ 57) for Naegleria spp. The identified species included Naegleria spp., Naegleria canariensis, and Naegleria clarki. N. fowleri was not found in Taiwan's reservoirs used for drinking purposes. The concentrations of Naegleria spp. in detected positive reservoir water samples were in the range of 599 and 3.1 × 10(3) cells/L. The presence or absence of Naegleria spp. within the reservoir water samples showed significant difference with the levels of water temperature. The presence of Naegleria spp. in reservoirs considered a potential public health threat if pathogenic species exist in reservoirs. More... »

PAGES

3765-3771

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00436-014-4042-2

DOI

http://dx.doi.org/10.1007/s00436-014-4042-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051321450

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25079704


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Ribosomal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Ribosomal Spacer", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drinking Water", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fresh Water", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Naegleria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Ribosomal, 5.8S", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Real-Time Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Taiwan", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Water Supply", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Chung Cheng University", 
          "id": "https://www.grid.ac/institutes/grid.412047.4", 
          "name": [
            "Department of Earth and Environmental Sciences, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kao", 
        "givenName": "Po-Min", 
        "id": "sg:person.01342553315.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342553315.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Chung Cheng University", 
          "id": "https://www.grid.ac/institutes/grid.412047.4", 
          "name": [
            "Department of Earth and Environmental Sciences, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hsu", 
        "givenName": "Bing-Mu", 
        "id": "sg:person.0605015334.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605015334.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Chung Cheng University", 
          "id": "https://www.grid.ac/institutes/grid.412047.4", 
          "name": [
            "Department of Ophthalmology, Cheng Hsin General Hospital, No.45, Chenghsin St., Paitou Dist., 112, Taipei, Taiwan, Republic of China", 
            "Department of Life Science, Institute of Molecular Biology, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hsu", 
        "givenName": "Tsui-Kang", 
        "id": "sg:person.01262103252.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262103252.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cheng Hsin General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.413846.c", 
          "name": [
            "General Surgery, Surgical Department, Cheng Hsin General Hospital, No.45, Chenghsin St., Paitou Dist., 112, Taipei, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chiu", 
        "givenName": "Yi-Chou", 
        "id": "sg:person.01115737415.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115737415.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yuanpei University", 
          "id": "https://www.grid.ac/institutes/grid.413051.2", 
          "name": [
            "Department of Environmental Engineering and Health, Yuanpei University of Science and Technology, No.306, Yuanpei St., 300, Hsinchu, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Chung-Liang", 
        "id": "sg:person.013065723761.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013065723761.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Chung Cheng University", 
          "id": "https://www.grid.ac/institutes/grid.412047.4", 
          "name": [
            "Department of Earth and Environmental Sciences, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ji", 
        "givenName": "Wen-Tsai", 
        "id": "sg:person.01044431042.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044431042.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cheng Shiu University", 
          "id": "https://www.grid.ac/institutes/grid.411282.c", 
          "name": [
            "Center for General Education, Cheng Shiu University, No.840, Chengcing Rd., Niaosong Dist., 833, Kaohsiung, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Shih-Wei", 
        "id": "sg:person.0613754042.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613754042.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Chung Cheng University", 
          "id": "https://www.grid.ac/institutes/grid.412047.4", 
          "name": [
            "Department of Earth and Environmental Sciences, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fan", 
        "givenName": "Cheng-Wei", 
        "id": "sg:person.016467432633.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016467432633.09"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.ijpara.2013.01.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001814694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-002-0707-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001921369", 
          "https://doi.org/10.1007/s00436-002-0707-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.69.10.5914-5918.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002039390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-009-1528-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004023573", 
          "https://doi.org/10.1007/s00436-009-1528-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-009-1528-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004023573", 
          "https://doi.org/10.1007/s00436-009-1528-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-009-1528-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004023573", 
          "https://doi.org/10.1007/s00436-009-1528-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-013-3290-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005767571", 
          "https://doi.org/10.1007/s00436-013-3290-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-013-3290-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005767571", 
          "https://doi.org/10.1007/s00436-013-3290-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.patbio.2012.03.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007258612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.patbio.2012.03.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007258612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-010-1815-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009244025", 
          "https://doi.org/10.1007/s00436-010-1815-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-010-1815-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009244025", 
          "https://doi.org/10.1007/s00436-010-1815-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ejop.2006.02.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013805649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijpara.2004.06.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015935835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.actatropica.2011.04.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020483952"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.watres.2009.04.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021143935"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-011-2366-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021150033", 
          "https://doi.org/10.1007/s00436-011-2366-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00154994", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024089675", 
          "https://doi.org/10.1007/bf00154994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00154994", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024089675", 
          "https://doi.org/10.1007/bf00154994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.exppara.2010.04.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028720016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1550-7408.2003.tb00614.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1550-7408.2003.tb00614.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/lam.12006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034297619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-006-0399-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035191616", 
          "https://doi.org/10.1007/s00436-006-0399-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-006-0399-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035191616", 
          "https://doi.org/10.1007/s00436-006-0399-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-006-0399-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035191616", 
          "https://doi.org/10.1007/s00436-006-0399-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-2920.2008.01693.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036747232"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.watres.2006.10.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039981074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00436-010-2120-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047363539", 
          "https://doi.org/10.1007/s00436-010-2120-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.meegid.2011.07.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048747547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.meegid.2011.07.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048747547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1078/s1438-4639(04)70031-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050268156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es400160k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055505900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es4040683", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055507000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es900432m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055516201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/es900432m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055516201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1061/(asce)ee.1943-7870.0000549", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057628608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077180194", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077180195", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081051100", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-10", 
    "datePublishedReg": "2014-10-01", 
    "description": "Naegleria spp. can be found in the natural aquatic environments. Naegleria fowleri can cause fatal infections in the central nervous system in humans and animals, and the most important source of infection is through direct water contact. In this study, PCR of 5.8S ribosomal RNA (rRNA) gene and internal transcribed spacer (ITS) region was performed in order to identify Naegleria isolates and quantify the Naegleria spp. by TaqMan real-time quantitative PCR in reservoir water samples. The occurrence of Naegleria spp. was investigated in 57 water samples from reservoirs with culture and PCR positive in 2 of them (3.5%), respectively. The total detection rate was 7.0% (4/ 57) for Naegleria spp. The identified species included Naegleria spp., Naegleria canariensis, and Naegleria clarki. N. fowleri was not found in Taiwan's reservoirs used for drinking purposes. The concentrations of Naegleria spp. in detected positive reservoir water samples were in the range of 599 and 3.1\u2009\u00d7\u200910(3) cells/L. The presence or absence of Naegleria spp. within the reservoir water samples showed significant difference with the levels of water temperature. The presence of Naegleria spp. in reservoirs considered a potential public health threat if pathogenic species exist in reservoirs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00436-014-4042-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096949", 
        "issn": [
          "0932-0113", 
          "1432-1955"
        ], 
        "name": "Parasitology Research", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "113"
      }
    ], 
    "name": "Application of TaqMan qPCR for the detection and monitoring of Naegleria species in reservoirs used as a source for drinking water", 
    "pagination": "3765-3771", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f478d543eefe505bd1e34364d7588cf16169f24d352ef14e0ae4122f423f1c9e"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25079704"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8703571"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00436-014-4042-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1051321450"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00436-014-4042-2", 
      "https://app.dimensions.ai/details/publication/pub.1051321450"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000595.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00436-014-4042-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00436-014-4042-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00436-014-4042-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00436-014-4042-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00436-014-4042-2'


 

This table displays all metadata directly associated to this object as RDF triples.

265 TRIPLES      21 PREDICATES      69 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00436-014-4042-2 schema:about N0ea4c2b7f98047118994533493c172d0
2 N44b00b96f7ee41109b3505ba1263092f
3 N48940934ed3b425189ee37335c172654
4 N8a3732af27144d51b53b26437db7b1b0
5 Nc74c4522e59946e98573acdae3bfc252
6 Ncc72e77ee0ab412db69aaef68e875e44
7 Ndccff8fd7ff2435fa7eecfdb4448792d
8 Nf2acee18dbe047ed892552c29a6b9fa2
9 Nf463654b97f04718941c33c52b90a1ee
10 Nf889dc4f3b6a4b19a549a429a448de8e
11 Nfe6013619c6647a4b36961f277365fb6
12 anzsrc-for:11
13 anzsrc-for:1108
14 schema:author Naeaea87bcd69470abc42afaff51dcf13
15 schema:citation sg:pub.10.1007/bf00154994
16 sg:pub.10.1007/s00436-002-0707-3
17 sg:pub.10.1007/s00436-006-0399-1
18 sg:pub.10.1007/s00436-009-1528-4
19 sg:pub.10.1007/s00436-010-1815-0
20 sg:pub.10.1007/s00436-010-2120-7
21 sg:pub.10.1007/s00436-011-2366-8
22 sg:pub.10.1007/s00436-013-3290-x
23 https://app.dimensions.ai/details/publication/pub.1077180194
24 https://app.dimensions.ai/details/publication/pub.1077180195
25 https://app.dimensions.ai/details/publication/pub.1081051100
26 https://doi.org/10.1016/j.actatropica.2011.04.016
27 https://doi.org/10.1016/j.ejop.2006.02.001
28 https://doi.org/10.1016/j.exppara.2010.04.011
29 https://doi.org/10.1016/j.ijpara.2004.06.004
30 https://doi.org/10.1016/j.ijpara.2013.01.012
31 https://doi.org/10.1016/j.meegid.2011.07.023
32 https://doi.org/10.1016/j.patbio.2012.03.002
33 https://doi.org/10.1016/j.watres.2006.10.003
34 https://doi.org/10.1016/j.watres.2009.04.002
35 https://doi.org/10.1021/es400160k
36 https://doi.org/10.1021/es4040683
37 https://doi.org/10.1021/es900432m
38 https://doi.org/10.1061/(asce)ee.1943-7870.0000549
39 https://doi.org/10.1078/s1438-4639(04)70031-0
40 https://doi.org/10.1111/j.1462-2920.2008.01693.x
41 https://doi.org/10.1111/j.1550-7408.2003.tb00614.x
42 https://doi.org/10.1111/lam.12006
43 https://doi.org/10.1128/aem.69.10.5914-5918.2003
44 schema:datePublished 2014-10
45 schema:datePublishedReg 2014-10-01
46 schema:description Naegleria spp. can be found in the natural aquatic environments. Naegleria fowleri can cause fatal infections in the central nervous system in humans and animals, and the most important source of infection is through direct water contact. In this study, PCR of 5.8S ribosomal RNA (rRNA) gene and internal transcribed spacer (ITS) region was performed in order to identify Naegleria isolates and quantify the Naegleria spp. by TaqMan real-time quantitative PCR in reservoir water samples. The occurrence of Naegleria spp. was investigated in 57 water samples from reservoirs with culture and PCR positive in 2 of them (3.5%), respectively. The total detection rate was 7.0% (4/ 57) for Naegleria spp. The identified species included Naegleria spp., Naegleria canariensis, and Naegleria clarki. N. fowleri was not found in Taiwan's reservoirs used for drinking purposes. The concentrations of Naegleria spp. in detected positive reservoir water samples were in the range of 599 and 3.1 × 10(3) cells/L. The presence or absence of Naegleria spp. within the reservoir water samples showed significant difference with the levels of water temperature. The presence of Naegleria spp. in reservoirs considered a potential public health threat if pathogenic species exist in reservoirs.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree false
50 schema:isPartOf N42fd5697ea7a44dba872e3312097d167
51 N8a5126434205482cb9a4120521546030
52 sg:journal.1096949
53 schema:name Application of TaqMan qPCR for the detection and monitoring of Naegleria species in reservoirs used as a source for drinking water
54 schema:pagination 3765-3771
55 schema:productId N1190808ca4e84a36924fdfa8be0f9034
56 N18a57816b12743dd99cf854e746530e2
57 N2c5ba3977c6c4774a6c28e7fd940acfa
58 N835efb2adc2342e89689fccf519211fd
59 Nd55cbc316edc4ee3be36cf1ca0523ab6
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051321450
61 https://doi.org/10.1007/s00436-014-4042-2
62 schema:sdDatePublished 2019-04-11T02:29
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher Ndc1e84bc02d447259b952fe424cfb6ba
65 schema:url http://link.springer.com/10.1007%2Fs00436-014-4042-2
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N0ea4c2b7f98047118994533493c172d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Taiwan
71 rdf:type schema:DefinedTerm
72 N1190808ca4e84a36924fdfa8be0f9034 schema:name dimensions_id
73 schema:value pub.1051321450
74 rdf:type schema:PropertyValue
75 N18a57816b12743dd99cf854e746530e2 schema:name readcube_id
76 schema:value f478d543eefe505bd1e34364d7588cf16169f24d352ef14e0ae4122f423f1c9e
77 rdf:type schema:PropertyValue
78 N2c5ba3977c6c4774a6c28e7fd940acfa schema:name nlm_unique_id
79 schema:value 8703571
80 rdf:type schema:PropertyValue
81 N42fd5697ea7a44dba872e3312097d167 schema:issueNumber 10
82 rdf:type schema:PublicationIssue
83 N44b00b96f7ee41109b3505ba1263092f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Real-Time Polymerase Chain Reaction
85 rdf:type schema:DefinedTerm
86 N48940934ed3b425189ee37335c172654 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Drinking Water
88 rdf:type schema:DefinedTerm
89 N5e94be017cd34848933e5db59b663e45 rdf:first sg:person.01262103252.21
90 rdf:rest Na8c0ef042d184f82bcf8c49d64af2a47
91 N67d8f436caee4d6c93fa128f4c03da0f rdf:first sg:person.0613754042.03
92 rdf:rest N69627d75e65d45bda243ba1a2f85bbc7
93 N69627d75e65d45bda243ba1a2f85bbc7 rdf:first sg:person.016467432633.09
94 rdf:rest rdf:nil
95 N6bb6770df3c24b68b62d8e541583ba10 rdf:first sg:person.0605015334.71
96 rdf:rest N5e94be017cd34848933e5db59b663e45
97 N835efb2adc2342e89689fccf519211fd schema:name pubmed_id
98 schema:value 25079704
99 rdf:type schema:PropertyValue
100 N8a3732af27144d51b53b26437db7b1b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name DNA, Ribosomal
102 rdf:type schema:DefinedTerm
103 N8a5126434205482cb9a4120521546030 schema:volumeNumber 113
104 rdf:type schema:PublicationVolume
105 N9f3f18b895aa4e41a992a83bdec191e0 rdf:first sg:person.01044431042.51
106 rdf:rest N67d8f436caee4d6c93fa128f4c03da0f
107 Na8c0ef042d184f82bcf8c49d64af2a47 rdf:first sg:person.01115737415.30
108 rdf:rest Ncafa7569204b470b9f8cd4436ed3f6ce
109 Naeaea87bcd69470abc42afaff51dcf13 rdf:first sg:person.01342553315.01
110 rdf:rest N6bb6770df3c24b68b62d8e541583ba10
111 Nc74c4522e59946e98573acdae3bfc252 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Animals
113 rdf:type schema:DefinedTerm
114 Ncafa7569204b470b9f8cd4436ed3f6ce rdf:first sg:person.013065723761.29
115 rdf:rest N9f3f18b895aa4e41a992a83bdec191e0
116 Ncc72e77ee0ab412db69aaef68e875e44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Water Supply
118 rdf:type schema:DefinedTerm
119 Nd55cbc316edc4ee3be36cf1ca0523ab6 schema:name doi
120 schema:value 10.1007/s00436-014-4042-2
121 rdf:type schema:PropertyValue
122 Ndc1e84bc02d447259b952fe424cfb6ba schema:name Springer Nature - SN SciGraph project
123 rdf:type schema:Organization
124 Ndccff8fd7ff2435fa7eecfdb4448792d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Fresh Water
126 rdf:type schema:DefinedTerm
127 Nf2acee18dbe047ed892552c29a6b9fa2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name DNA, Ribosomal Spacer
129 rdf:type schema:DefinedTerm
130 Nf463654b97f04718941c33c52b90a1ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name RNA, Ribosomal, 5.8S
132 rdf:type schema:DefinedTerm
133 Nf889dc4f3b6a4b19a549a429a448de8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Humans
135 rdf:type schema:DefinedTerm
136 Nfe6013619c6647a4b36961f277365fb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Naegleria
138 rdf:type schema:DefinedTerm
139 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
140 schema:name Medical and Health Sciences
141 rdf:type schema:DefinedTerm
142 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
143 schema:name Medical Microbiology
144 rdf:type schema:DefinedTerm
145 sg:journal.1096949 schema:issn 0932-0113
146 1432-1955
147 schema:name Parasitology Research
148 rdf:type schema:Periodical
149 sg:person.01044431042.51 schema:affiliation https://www.grid.ac/institutes/grid.412047.4
150 schema:familyName Ji
151 schema:givenName Wen-Tsai
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044431042.51
153 rdf:type schema:Person
154 sg:person.01115737415.30 schema:affiliation https://www.grid.ac/institutes/grid.413846.c
155 schema:familyName Chiu
156 schema:givenName Yi-Chou
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115737415.30
158 rdf:type schema:Person
159 sg:person.01262103252.21 schema:affiliation https://www.grid.ac/institutes/grid.412047.4
160 schema:familyName Hsu
161 schema:givenName Tsui-Kang
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262103252.21
163 rdf:type schema:Person
164 sg:person.013065723761.29 schema:affiliation https://www.grid.ac/institutes/grid.413051.2
165 schema:familyName Chang
166 schema:givenName Chung-Liang
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013065723761.29
168 rdf:type schema:Person
169 sg:person.01342553315.01 schema:affiliation https://www.grid.ac/institutes/grid.412047.4
170 schema:familyName Kao
171 schema:givenName Po-Min
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342553315.01
173 rdf:type schema:Person
174 sg:person.016467432633.09 schema:affiliation https://www.grid.ac/institutes/grid.412047.4
175 schema:familyName Fan
176 schema:givenName Cheng-Wei
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016467432633.09
178 rdf:type schema:Person
179 sg:person.0605015334.71 schema:affiliation https://www.grid.ac/institutes/grid.412047.4
180 schema:familyName Hsu
181 schema:givenName Bing-Mu
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605015334.71
183 rdf:type schema:Person
184 sg:person.0613754042.03 schema:affiliation https://www.grid.ac/institutes/grid.411282.c
185 schema:familyName Huang
186 schema:givenName Shih-Wei
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613754042.03
188 rdf:type schema:Person
189 sg:pub.10.1007/bf00154994 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024089675
190 https://doi.org/10.1007/bf00154994
191 rdf:type schema:CreativeWork
192 sg:pub.10.1007/s00436-002-0707-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001921369
193 https://doi.org/10.1007/s00436-002-0707-3
194 rdf:type schema:CreativeWork
195 sg:pub.10.1007/s00436-006-0399-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035191616
196 https://doi.org/10.1007/s00436-006-0399-1
197 rdf:type schema:CreativeWork
198 sg:pub.10.1007/s00436-009-1528-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004023573
199 https://doi.org/10.1007/s00436-009-1528-4
200 rdf:type schema:CreativeWork
201 sg:pub.10.1007/s00436-010-1815-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009244025
202 https://doi.org/10.1007/s00436-010-1815-0
203 rdf:type schema:CreativeWork
204 sg:pub.10.1007/s00436-010-2120-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047363539
205 https://doi.org/10.1007/s00436-010-2120-7
206 rdf:type schema:CreativeWork
207 sg:pub.10.1007/s00436-011-2366-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021150033
208 https://doi.org/10.1007/s00436-011-2366-8
209 rdf:type schema:CreativeWork
210 sg:pub.10.1007/s00436-013-3290-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005767571
211 https://doi.org/10.1007/s00436-013-3290-x
212 rdf:type schema:CreativeWork
213 https://app.dimensions.ai/details/publication/pub.1077180194 schema:CreativeWork
214 https://app.dimensions.ai/details/publication/pub.1077180195 schema:CreativeWork
215 https://app.dimensions.ai/details/publication/pub.1081051100 schema:CreativeWork
216 https://doi.org/10.1016/j.actatropica.2011.04.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020483952
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.ejop.2006.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013805649
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.exppara.2010.04.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028720016
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.ijpara.2004.06.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015935835
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.ijpara.2013.01.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001814694
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.meegid.2011.07.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048747547
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.patbio.2012.03.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007258612
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.watres.2006.10.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039981074
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.watres.2009.04.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021143935
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/es400160k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055505900
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1021/es4040683 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055507000
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1021/es900432m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055516201
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1061/(asce)ee.1943-7870.0000549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057628608
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1078/s1438-4639(04)70031-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050268156
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1111/j.1462-2920.2008.01693.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036747232
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1111/j.1550-7408.2003.tb00614.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032555186
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1111/lam.12006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034297619
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1128/aem.69.10.5914-5918.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002039390
251 rdf:type schema:CreativeWork
252 https://www.grid.ac/institutes/grid.411282.c schema:alternateName Cheng Shiu University
253 schema:name Center for General Education, Cheng Shiu University, No.840, Chengcing Rd., Niaosong Dist., 833, Kaohsiung, Taiwan, Republic of China
254 rdf:type schema:Organization
255 https://www.grid.ac/institutes/grid.412047.4 schema:alternateName National Chung Cheng University
256 schema:name Department of Earth and Environmental Sciences, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China
257 Department of Life Science, Institute of Molecular Biology, National Chung Cheng University, No.168, Sec. 1, University Rd., Minhsiung Township, 621, Chiayi, Taiwan, Republic of China
258 Department of Ophthalmology, Cheng Hsin General Hospital, No.45, Chenghsin St., Paitou Dist., 112, Taipei, Taiwan, Republic of China
259 rdf:type schema:Organization
260 https://www.grid.ac/institutes/grid.413051.2 schema:alternateName Yuanpei University
261 schema:name Department of Environmental Engineering and Health, Yuanpei University of Science and Technology, No.306, Yuanpei St., 300, Hsinchu, Taiwan, Republic of China
262 rdf:type schema:Organization
263 https://www.grid.ac/institutes/grid.413846.c schema:alternateName Cheng Hsin General Hospital
264 schema:name General Surgery, Surgical Department, Cheng Hsin General Hospital, No.45, Chenghsin St., Paitou Dist., 112, Taipei, Taiwan, Republic of China
265 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...