Cloning and characterization of Taenia saginata paramyosin cDNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-09

AUTHORS

Elizabeth Ferrer, Eva Moyano, Laura Benitez, Luis Miguel González, Denise Bryce, Mildred Foster-Cuevas, Iris Dávila, Maria Milagros Cortéz, Leslie J. S. Harrison, R. Michael E. Parkhouse, Teresa Gárate

ABSTRACT

A lambdaZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule. More... »

PAGES

60-67

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00436-003-0895-5

DOI

http://dx.doi.org/10.1007/s00436-003-0895-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016085750

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12898225


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00436-003-0895-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00436-003-0895-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00436-003-0895-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00436-003-0895-5'


 

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326 Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, Oxford, England
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