Invasive potentials of gastric carcinoma cell lines: role of alpha 2 and alpha 6 integrins in invasion. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-06-16

AUTHORS

N Koike, T Todoroki, H Komano, T Shimokama, S Ban, T Ohno, K Fukao, T Watanabe

ABSTRACT

The potentials of the two major histological types of gastric carcinoma to invade through extracellular matrices were studied with cell lines. We found that the invasive potential of intestinal-type carcinoma cells (MKN-28 and MKN-74) were higher than those of diffuse-type carcinoma cells (MKN-45 and KATO-III). To investigate whether the alpha 2 and alpha 6 integrin adhesion molecules are responsible for, or involved in carcinoma invasion. We further studied alpha 2 and alpha 6 expression patterns in these two types of cell line. Although fluorescence-activated cell sorting analysis revealed that all cells examined invariably expressed these integrin molecules, their expressional patterns were different among different cell lines. The intestinal-type carcinoma cells expressed integrins mainly along the cell-cell contact region, whereas the diffuse-type carcinoma cells showed a diffuse cytoplasmic pattern of integrin expression. Invasion by MKN-28, MKN-74 and MKN-45 cells through reconstituted basement membrane or type I collagen gel was significantly inhibited (P < 0.05) by 50 micrograms/ml anti-(alpha 2 integrin) or anti-(alpha 6 integrin) monoclonal antibodies. Our results suggest that active invasiveness is stronger in the intestinal-type than in the diffuse-type carcinoma cells and that alpha 2 and alpha 6 integrins play important roles in invasion of both types of gastric carcinoma cell lines. More... »

PAGES

310-6

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004320050063

DOI

http://dx.doi.org/10.1007/s004320050063

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1054516312

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9222296


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Separation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Integrin alpha2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Integrin alpha6", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Invasiveness", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stomach Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Surgery, University of Tsukuba, Ibaraki, Japan.", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Department of Surgery, University of Tsukuba, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koike", 
        "givenName": "N", 
        "id": "sg:person.01330372147.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330372147.74"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Todoroki", 
        "givenName": "T", 
        "id": "sg:person.011557753737.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011557753737.86"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Komano", 
        "givenName": "H", 
        "id": "sg:person.01305436341.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01305436341.34"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Shimokama", 
        "givenName": "T", 
        "id": "sg:person.01271015273.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01271015273.11"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Ban", 
        "givenName": "S", 
        "id": "sg:person.01077775011.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077775011.21"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Ohno", 
        "givenName": "T", 
        "id": "sg:person.0662433203.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662433203.59"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Fukao", 
        "givenName": "K", 
        "id": "sg:person.01234530531.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234530531.38"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Watanabe", 
        "givenName": "T", 
        "id": "sg:person.012664624074.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/bjc.1992.250", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041198140", 
          "https://doi.org/10.1038/bjc.1992.250"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1997-06-16", 
    "datePublishedReg": "1997-06-16", 
    "description": "The potentials of the two major histological types of gastric carcinoma to invade through extracellular matrices were studied with cell lines. We found that the invasive potential of intestinal-type carcinoma cells (MKN-28 and MKN-74) were higher than those of diffuse-type carcinoma cells (MKN-45 and KATO-III). To investigate whether the alpha 2 and alpha 6 integrin adhesion molecules are responsible for, or involved in carcinoma invasion. We further studied alpha 2 and alpha 6 expression patterns in these two types of cell line. Although fluorescence-activated cell sorting analysis revealed that all cells examined invariably expressed these integrin molecules, their expressional patterns were different among different cell lines. The intestinal-type carcinoma cells expressed integrins mainly along the cell-cell contact region, whereas the diffuse-type carcinoma cells showed a diffuse cytoplasmic pattern of integrin expression. Invasion by MKN-28, MKN-74 and MKN-45 cells through reconstituted basement membrane or type I collagen gel was significantly inhibited (P < 0.05) by 50 micrograms/ml anti-(alpha 2 integrin) or anti-(alpha 6 integrin) monoclonal antibodies. Our results suggest that active invasiveness is stronger in the intestinal-type than in the diffuse-type carcinoma cells and that alpha 2 and alpha 6 integrins play important roles in invasion of both types of gastric carcinoma cell lines.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s004320050063", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1313647", 
        "issn": [
          "0171-5216", 
          "1432-1335"
        ], 
        "name": "Journal of Cancer Research and Clinical Oncology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "123"
      }
    ], 
    "keywords": [
      "gastric carcinoma cell lines", 
      "alpha 6 integrin", 
      "carcinoma cells", 
      "alpha 2", 
      "carcinoma cell lines", 
      "cell lines", 
      "major histological types", 
      "integrin adhesion molecules", 
      "invasive potential", 
      "MKN-45 cells", 
      "active invasiveness", 
      "diffuse cytoplasmic pattern", 
      "fluorescence-activated cell", 
      "histological type", 
      "gastric carcinoma", 
      "anti-monoclonal antibodies", 
      "MKN-74", 
      "micrograms/", 
      "cytoplasmic pattern", 
      "MKN-28", 
      "carcinoma invasion", 
      "adhesion molecules", 
      "integrin expression", 
      "basement membrane", 
      "invasion", 
      "type I", 
      "integrin molecules", 
      "different cell lines", 
      "cells", 
      "expressional patterns", 
      "integrins", 
      "expression patterns", 
      "extracellular matrix", 
      "carcinoma", 
      "important role", 
      "antibodies", 
      "cell-cell contact regions", 
      "invasiveness", 
      "role", 
      "patterns", 
      "expression", 
      "lines", 
      "types", 
      "potential", 
      "molecules", 
      "membrane", 
      "analysis", 
      "results", 
      "region", 
      "gel", 
      "contact region", 
      "matrix"
    ], 
    "name": "Invasive potentials of gastric carcinoma cell lines: role of alpha 2 and alpha 6 integrins in invasion.", 
    "pagination": "310-6", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1054516312"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s004320050063"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9222296"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s004320050063", 
      "https://app.dimensions.ai/details/publication/pub.1054516312"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_298.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s004320050063"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s004320050063'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s004320050063'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s004320050063'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s004320050063'


 

This table displays all metadata directly associated to this object as RDF triples.

196 TRIPLES      22 PREDICATES      88 URIs      79 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s004320050063 schema:about N0020c3b0e7ff471f9017c678cdd7992e
2 N04b15948e9a14223b12a6a2f28f6eac7
3 N192de02e53154fb6aa89939822924508
4 N50a14f1ff8a94b83a8726c621fb6404e
5 N7ad34c8b92e848b89251ce7802d524a7
6 N919860abdefb4ee5aa178d4553af3b3a
7 Nabfcd357db14447cbc190dd96f80b918
8 Nb6e62730cc5a4632a8e1ff1383a89043
9 Ndcc4412bf96548ca9bd30ffa24d9d4e4
10 anzsrc-for:11
11 anzsrc-for:1112
12 schema:author N3075e98e07e54271b4f56baa38837272
13 schema:citation sg:pub.10.1038/bjc.1992.250
14 schema:datePublished 1997-06-16
15 schema:datePublishedReg 1997-06-16
16 schema:description The potentials of the two major histological types of gastric carcinoma to invade through extracellular matrices were studied with cell lines. We found that the invasive potential of intestinal-type carcinoma cells (MKN-28 and MKN-74) were higher than those of diffuse-type carcinoma cells (MKN-45 and KATO-III). To investigate whether the alpha 2 and alpha 6 integrin adhesion molecules are responsible for, or involved in carcinoma invasion. We further studied alpha 2 and alpha 6 expression patterns in these two types of cell line. Although fluorescence-activated cell sorting analysis revealed that all cells examined invariably expressed these integrin molecules, their expressional patterns were different among different cell lines. The intestinal-type carcinoma cells expressed integrins mainly along the cell-cell contact region, whereas the diffuse-type carcinoma cells showed a diffuse cytoplasmic pattern of integrin expression. Invasion by MKN-28, MKN-74 and MKN-45 cells through reconstituted basement membrane or type I collagen gel was significantly inhibited (P < 0.05) by 50 micrograms/ml anti-(alpha 2 integrin) or anti-(alpha 6 integrin) monoclonal antibodies. Our results suggest that active invasiveness is stronger in the intestinal-type than in the diffuse-type carcinoma cells and that alpha 2 and alpha 6 integrins play important roles in invasion of both types of gastric carcinoma cell lines.
17 schema:genre article
18 schema:inLanguage en
19 schema:isAccessibleForFree false
20 schema:isPartOf N084c342cef7544f7bf9ececffa55df52
21 N37055d8c00b941c7a596db2307f6f5c7
22 sg:journal.1313647
23 schema:keywords MKN-28
24 MKN-45 cells
25 MKN-74
26 active invasiveness
27 adhesion molecules
28 alpha 2
29 alpha 6 integrin
30 analysis
31 anti-monoclonal antibodies
32 antibodies
33 basement membrane
34 carcinoma
35 carcinoma cell lines
36 carcinoma cells
37 carcinoma invasion
38 cell lines
39 cell-cell contact regions
40 cells
41 contact region
42 cytoplasmic pattern
43 different cell lines
44 diffuse cytoplasmic pattern
45 expression
46 expression patterns
47 expressional patterns
48 extracellular matrix
49 fluorescence-activated cell
50 gastric carcinoma
51 gastric carcinoma cell lines
52 gel
53 histological type
54 important role
55 integrin adhesion molecules
56 integrin expression
57 integrin molecules
58 integrins
59 invasion
60 invasive potential
61 invasiveness
62 lines
63 major histological types
64 matrix
65 membrane
66 micrograms/
67 molecules
68 patterns
69 potential
70 region
71 results
72 role
73 type I
74 types
75 schema:name Invasive potentials of gastric carcinoma cell lines: role of alpha 2 and alpha 6 integrins in invasion.
76 schema:pagination 310-6
77 schema:productId N155f024f51044c57b74df98b6daa66bb
78 Na6768a83969a41428808bc2a44c4608e
79 Nd574d21263bf491abd63f2aa4d73e583
80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054516312
81 https://doi.org/10.1007/s004320050063
82 schema:sdDatePublished 2022-06-01T22:03
83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
84 schema:sdPublisher N763f316ed69b40d69d6a5ded8778ea62
85 schema:url https://doi.org/10.1007/s004320050063
86 sgo:license sg:explorer/license/
87 sgo:sdDataset articles
88 rdf:type schema:ScholarlyArticle
89 N0020c3b0e7ff471f9017c678cdd7992e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Integrin alpha2
91 rdf:type schema:DefinedTerm
92 N04b15948e9a14223b12a6a2f28f6eac7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Tumor Cells, Cultured
94 rdf:type schema:DefinedTerm
95 N084c342cef7544f7bf9ececffa55df52 schema:volumeNumber 123
96 rdf:type schema:PublicationVolume
97 N155f024f51044c57b74df98b6daa66bb schema:name doi
98 schema:value 10.1007/s004320050063
99 rdf:type schema:PropertyValue
100 N192de02e53154fb6aa89939822924508 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Stomach Neoplasms
102 rdf:type schema:DefinedTerm
103 N2d7877196efc411e83664e2ee0edc928 rdf:first sg:person.01271015273.11
104 rdf:rest N72f3ad8c424b4d5ba00b5e361d357859
105 N3075e98e07e54271b4f56baa38837272 rdf:first sg:person.01330372147.74
106 rdf:rest N6965046a5eb540009ecd1c5b1c582d85
107 N37055d8c00b941c7a596db2307f6f5c7 schema:issueNumber 6
108 rdf:type schema:PublicationIssue
109 N50a14f1ff8a94b83a8726c621fb6404e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Carcinoma
111 rdf:type schema:DefinedTerm
112 N6965046a5eb540009ecd1c5b1c582d85 rdf:first sg:person.011557753737.86
113 rdf:rest N6ec13ba525ab4a038911c9e4eb2da6f2
114 N6ec13ba525ab4a038911c9e4eb2da6f2 rdf:first sg:person.01305436341.34
115 rdf:rest N2d7877196efc411e83664e2ee0edc928
116 N72f3ad8c424b4d5ba00b5e361d357859 rdf:first sg:person.01077775011.21
117 rdf:rest N9eaea5b9af8149c18a60e72cfdebdeed
118 N763f316ed69b40d69d6a5ded8778ea62 schema:name Springer Nature - SN SciGraph project
119 rdf:type schema:Organization
120 N7ad34c8b92e848b89251ce7802d524a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Neoplasm Invasiveness
122 rdf:type schema:DefinedTerm
123 N84096c623d8243c19d393df37001c494 rdf:first sg:person.012664624074.29
124 rdf:rest rdf:nil
125 N919860abdefb4ee5aa178d4553af3b3a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Cell Separation
127 rdf:type schema:DefinedTerm
128 N9eaea5b9af8149c18a60e72cfdebdeed rdf:first sg:person.0662433203.59
129 rdf:rest Nabb49497744443488394889f729bfa06
130 Na6768a83969a41428808bc2a44c4608e schema:name pubmed_id
131 schema:value 9222296
132 rdf:type schema:PropertyValue
133 Nabb49497744443488394889f729bfa06 rdf:first sg:person.01234530531.38
134 rdf:rest N84096c623d8243c19d393df37001c494
135 Nabfcd357db14447cbc190dd96f80b918 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Antigens, CD
137 rdf:type schema:DefinedTerm
138 Nb6e62730cc5a4632a8e1ff1383a89043 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Integrin alpha6
140 rdf:type schema:DefinedTerm
141 Nd574d21263bf491abd63f2aa4d73e583 schema:name dimensions_id
142 schema:value pub.1054516312
143 rdf:type schema:PropertyValue
144 Ndcc4412bf96548ca9bd30ffa24d9d4e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Humans
146 rdf:type schema:DefinedTerm
147 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
148 schema:name Medical and Health Sciences
149 rdf:type schema:DefinedTerm
150 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
151 schema:name Oncology and Carcinogenesis
152 rdf:type schema:DefinedTerm
153 sg:journal.1313647 schema:issn 0171-5216
154 1432-1335
155 schema:name Journal of Cancer Research and Clinical Oncology
156 schema:publisher Springer Nature
157 rdf:type schema:Periodical
158 sg:person.01077775011.21 schema:familyName Ban
159 schema:givenName S
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077775011.21
161 rdf:type schema:Person
162 sg:person.011557753737.86 schema:familyName Todoroki
163 schema:givenName T
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011557753737.86
165 rdf:type schema:Person
166 sg:person.01234530531.38 schema:familyName Fukao
167 schema:givenName K
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234530531.38
169 rdf:type schema:Person
170 sg:person.012664624074.29 schema:familyName Watanabe
171 schema:givenName T
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29
173 rdf:type schema:Person
174 sg:person.01271015273.11 schema:familyName Shimokama
175 schema:givenName T
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01271015273.11
177 rdf:type schema:Person
178 sg:person.01305436341.34 schema:familyName Komano
179 schema:givenName H
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01305436341.34
181 rdf:type schema:Person
182 sg:person.01330372147.74 schema:affiliation grid-institutes:grid.20515.33
183 schema:familyName Koike
184 schema:givenName N
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330372147.74
186 rdf:type schema:Person
187 sg:person.0662433203.59 schema:familyName Ohno
188 schema:givenName T
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662433203.59
190 rdf:type schema:Person
191 sg:pub.10.1038/bjc.1992.250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041198140
192 https://doi.org/10.1038/bjc.1992.250
193 rdf:type schema:CreativeWork
194 grid-institutes:grid.20515.33 schema:alternateName Department of Surgery, University of Tsukuba, Ibaraki, Japan.
195 schema:name Department of Surgery, University of Tsukuba, Ibaraki, Japan.
196 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...