Mina53, a novel c-Myc target gene, is frequently expressed in lung cancers and exerts oncogenic property in NIH/3T3 cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-09-16

AUTHORS

Kazutoshi Komiya, Naoko Sueoka-Aragane, Akemi Sato, Takashi Hisatomi, Toru Sakuragi, Masahiro Mitsuoka, Toshimi Sato, Shinichiro Hayashi, Hiroto Izumi, Makoto Tsuneoka, Eisaburo Sueoka

ABSTRACT

PurposeMina53, whose expression is directly induced by c-Myc, is overexpressed in various cancers and plays an important role in cell growth. To clarify the involvement of Mina53 in lung cancers, we investigated its expression in human lung cancer tissues as well as in various lung cancer cell lines.MethodsMina53 expression was determined by real-time RT-PCR, western blotting, and immunohistochemistry using lung cancer cell lines, normal human bronchial epithelial cells, and lung cancer tissues. Biological effects of Mina53 were evaluated by soft agar colony formation assay and tumorigenicity in nude mice using Mina53-transfected NIH/3T3 cells. cDNA microarray analysis was performed to determine the gene alteration by Mina53 and confirmation was made using real-time RT-PCR with mina53 expression plasmid or mina53 shRNA-transfected NIH/3T3 cells.ResultsWe observed that 62% of patients evidenced overexpression of Mina53 from the early clinical stages of lung cancer. Differences according to gender, smoking status, or histologic type were not statistically significant. Forced expression of Mina53 in NIH/3T3 cells induced cell transformation, and mina53-transfected NIH/3T3 clones produced tumors in nude mice, demonstrating that Mina53 has oncogenic potential. cDNA microarray revealed that 254 genes had altered expression in a mina53-transfected NIH/3T3 clone. Mina53 regulates several genes related to cell adhesion and metabolism, which have also been reported to be regulated by c-Myc. Genes regulated by Mina53, but not by c-Myc included cytokine/growth factor related genes such as EGFR, IL-6, and HGF.ConclusionOur results suggest that Mina53 plays an important role in carcinogenesis and may be a target for cancer prevention. More... »

PAGES

465-473

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00432-009-0679-0

DOI

http://dx.doi.org/10.1007/s00432-009-0679-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024985556

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19756735


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33 schema:description PurposeMina53, whose expression is directly induced by c-Myc, is overexpressed in various cancers and plays an important role in cell growth. To clarify the involvement of Mina53 in lung cancers, we investigated its expression in human lung cancer tissues as well as in various lung cancer cell lines.MethodsMina53 expression was determined by real-time RT-PCR, western blotting, and immunohistochemistry using lung cancer cell lines, normal human bronchial epithelial cells, and lung cancer tissues. Biological effects of Mina53 were evaluated by soft agar colony formation assay and tumorigenicity in nude mice using Mina53-transfected NIH/3T3 cells. cDNA microarray analysis was performed to determine the gene alteration by Mina53 and confirmation was made using real-time RT-PCR with mina53 expression plasmid or mina53 shRNA-transfected NIH/3T3 cells.ResultsWe observed that 62% of patients evidenced overexpression of Mina53 from the early clinical stages of lung cancer. Differences according to gender, smoking status, or histologic type were not statistically significant. Forced expression of Mina53 in NIH/3T3 cells induced cell transformation, and mina53-transfected NIH/3T3 clones produced tumors in nude mice, demonstrating that Mina53 has oncogenic potential. cDNA microarray revealed that 254 genes had altered expression in a mina53-transfected NIH/3T3 clone. Mina53 regulates several genes related to cell adhesion and metabolism, which have also been reported to be regulated by c-Myc. Genes regulated by Mina53, but not by c-Myc included cytokine/growth factor related genes such as EGFR, IL-6, and HGF.ConclusionOur results suggest that Mina53 plays an important role in carcinogenesis and may be a target for cancer prevention.
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39 schema:keywords ConclusionOur results
40 EGFR
41 HGF
42 IL-6
43 Mina53
44 NIH/3T3 cells
45 NIH/3T3 clones
46 RT-PCR
47 ResultsWe
48 Western blotting
49 adhesion
50 alterations
51 analysis
52 assays
53 biological effects
54 blotting
55 bronchial epithelial cells
56 c-Myc
57 c-Myc target genes
58 cDNA microarray
59 cDNA microarray analysis
60 cancer
61 cancer cell lines
62 cancer prevention
63 cancer tissues
64 carcinogenesis
65 cell adhesion
66 cell growth
67 cell lines
68 cell transformation
69 cells
70 clinical stage
71 clones
72 colony formation assays
73 confirmation
74 cytokines/growth factors
75 differences
76 early clinical stage
77 effect
78 epithelial cells
79 exerts oncogenic properties
80 expression
81 expression of Mina53
82 expression plasmid
83 factors
84 formation assays
85 gender
86 gene alterations
87 genes
88 growth
89 growth factor
90 histologic type
91 human bronchial epithelial cells
92 human lung cancer tissues
93 immunohistochemistry
94 important role
95 involvement
96 lines
97 lung cancer
98 lung cancer cell lines
99 lung cancer tissues
100 metabolism
101 mice
102 microarray
103 microarray analysis
104 normal human bronchial epithelial cells
105 novel c-Myc target gene
106 nude mice
107 oncogenic potential
108 oncogenic properties
109 overexpression
110 patients
111 plasmid
112 potential
113 prevention
114 properties
115 real-time RT-PCR
116 results
117 role
118 smoking status
119 soft agar colony formation assays
120 stage
121 status
122 target
123 target genes
124 tissue
125 transformation
126 tumorigenicity
127 tumors
128 types
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