Ontology type: schema:ScholarlyArticle
1999-11
AUTHORSMachiko Nishio, Masato Tsurudome, Morihiro Ito, Mitsuo Kawano, Shigeru Kusagawa, Hiroshi Komada, Yasuhiko Ito
ABSTRACTTwo monoclonal antibodies (mAbs) specific for the human parainfluenza virus type 2 (hPIV-2) V protein were obtained by immunizing mice with the V protein recombinantly expressed in Escherichia coli. Both mAbs were found to react with the V protein in ELISA and in Western blot analysis. Using these mAbs and previously obtained mAbs specific for hPIV-2 nucleoprotein (NP) or hPIV-2 phospho-(P) protein, we examined the intracellular distributions of the V, P and NP proteins in hPIV-2-infected cells by indirect immunofluorescence analyses. The P and NP proteins were organized in numerous granules in the cytoplasm of hPIV-2 infected cells. In contrast, the V protein showed diffuse nuclear and cytoplasm distributions. More... »
PAGES79-82
http://scigraph.springernature.com/pub.10.1007/s004300050108
DOIhttp://dx.doi.org/10.1007/s004300050108
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/10753059
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