Ontology type: schema:ScholarlyArticle
2006-03
AUTHORSMachiko Nishio, Masato Tsurudome, Morihiro Ito, Yasuhiko Ito
ABSTRACTWe have shown that the P and V proteins of human parainfluenza virus type 2 (hPIV-2) bind to genomic RNA by using Northwestern blot analysis. To identify the RNA-binding regions on the P and V proteins, we used a set of deletion mutants produced in Escherichia coli. One region required for the RNA-binding was found in the P-V common domain (aa 1-82). Others were found in the P protein-specific region (aa 249-354) and the V protein-specific region (aa 176-225). In addition, we have shown that substitutions of some basic residues with alanines in these regions abrogate RNA-binding by the P or V proteins. Intriguingly, the P and V proteins of hPIV2 can selectively bind to the viral RNA under our experimental conditions. More... »
PAGES29-36
http://scigraph.springernature.com/pub.10.1007/s00430-005-0244-7
DOIhttp://dx.doi.org/10.1007/s00430-005-0244-7
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