Safe transportation of formalin-fixed liquid-free pathology specimens View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-07

AUTHORS

J. Metovic, L. Bertero, C. Musuraca, F. Veneziano, L. Annaratone, S. Mariani, P. Cassoni, G. Bussolati, Mauro Papotti

ABSTRACT

Diagnostic pathology activities are largely based on fixation of tissues in 4% formaldehyde, which has recently been re-classified as a carcinogenic compound and banned in several countries. Hospitals that do not have in-house pathology services need to send surgical and biopsy specimens to referral centers. These are generally transferred in liquid containers, under suboptimal safety conditions, as accidental spillage of potentially dangerous substances may occur. A safe, innovative, two-step procedure for pathology sample transportation is presented. Formalin-fixed material from ten surgical cases was dissected (including surrogate biopsies) and preserved in liquid-free plastic bags under vacuum for up to 30 days and subsequently processed for conventional histology, several immunohistochemical markers, and molecular tests (e.g., RAS mutation). The data were compared with the corresponding routine analyses. Formalin-fixed specimens after up to 30 days under vacuum storage gave equivalent results compared to standard histopathological slides and molecular tests, regarding both hematoxylin-eosin, immuno-stained slides and also nucleic acid extracted for molecular tests. The proposal of under-vacuum sealing pathology specimens that were previously formalin fixed can be adopted to transfer liquid-free biopsy and surgical specimens to referral pathology services. In fact, it is easy to perform, less expensive (both plastic bags and domestic-type vacuum chamber machines are at affordable costs), and above all is fully safe and adequate in the pre-analytical processing of pathology specimens. More... »

PAGES

105-113

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00428-018-2383-4

DOI

http://dx.doi.org/10.1007/s00428-018-2383-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104369766

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29860620


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biopsy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Formaldehyde", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hematoxylin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Paraffin Embedding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Specimen Handling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Fixation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Preservation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transportation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vacuum", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Oncology, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Metovic", 
        "givenName": "J.", 
        "id": "sg:person.015742566052.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015742566052.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Medical Sciences, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bertero", 
        "givenName": "L.", 
        "id": "sg:person.01141770554.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141770554.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Oncology, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Musuraca", 
        "givenName": "C.", 
        "id": "sg:person.012315325032.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012315325032.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Medical Sciences, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Veneziano", 
        "givenName": "F.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Medical Sciences, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Annaratone", 
        "givenName": "L.", 
        "id": "sg:person.01340627506.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340627506.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Medical Sciences, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mariani", 
        "givenName": "S.", 
        "id": "sg:person.01266642530.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266642530.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Medical Sciences, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cassoni", 
        "givenName": "P.", 
        "id": "sg:person.01215602014.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215602014.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Medical Sciences, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bussolati", 
        "givenName": "G.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Turin", 
          "id": "https://www.grid.ac/institutes/grid.7605.4", 
          "name": [
            "Pathology Unit, Department of Oncology, University of Turin and Citt\u00e0 della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy", 
            "Anatomia Patologica, Universit\u00e0 di Torino, Via Santena 7, 10126, Torino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Papotti", 
        "givenName": "Mauro", 
        "id": "sg:person.0633774363.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633774363.72"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.mrrev.2011.07.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009037272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00428-006-0233-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009364868", 
          "https://doi.org/10.1007/s00428-006-0233-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00428-006-0233-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009364868", 
          "https://doi.org/10.1007/s00428-006-0233-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1470-2045(12)70598-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009894380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00428-007-0529-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013120467", 
          "https://doi.org/10.1007/s00428-007-0529-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00428-007-0529-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013120467", 
          "https://doi.org/10.1007/s00428-007-0529-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.scitotenv.2010.04.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013147790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/33.8.3894502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016604505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/33.8.3894502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016604505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-319-13957-9_2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018049697", 
          "https://doi.org/10.1007/978-3-319-13957-9_2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.2009.117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021192460", 
          "https://doi.org/10.1038/modpathol.2009.117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.2009.117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021192460", 
          "https://doi.org/10.1038/modpathol.2009.117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0168466", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021753001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1055-9965.epi-09-0762", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028958087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/08923973.2016.1248844", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036364750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1470-2045(13)70109-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036459827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.2574", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038282602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.2574", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038282602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10520290701375302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038961766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-61779-286-1_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041056695", 
          "https://doi.org/10.1007/978-1-61779-286-1_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-61779-286-1_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041056695", 
          "https://doi.org/10.1007/978-1-61779-286-1_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6602889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049332317", 
          "https://doi.org/10.1038/sj.bjc.6602889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6602889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049332317", 
          "https://doi.org/10.1038/sj.bjc.6602889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/pdb.prot4986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060410923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075053673", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1043/1543-2165-132.12.1929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077819148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1043/2008-0432-ccr.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078340665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/bjc.2017.170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086031089", 
          "https://doi.org/10.1038/bjc.2017.170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0182965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091143093"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-07", 
    "datePublishedReg": "2018-07-01", 
    "description": "Diagnostic pathology activities are largely based on fixation of tissues in 4% formaldehyde, which has recently been re-classified as a carcinogenic compound and banned in several countries. Hospitals that do not have in-house pathology services need to send surgical and biopsy specimens to referral centers. These are generally transferred in liquid containers, under suboptimal safety conditions, as accidental spillage of potentially dangerous substances may occur. A safe, innovative, two-step procedure for pathology sample transportation is presented. Formalin-fixed material from ten surgical cases was dissected (including surrogate biopsies) and preserved in liquid-free plastic bags under vacuum for up to 30\u00a0days and subsequently processed for conventional histology, several immunohistochemical markers, and molecular tests (e.g., RAS mutation). The data were compared with the corresponding routine analyses. Formalin-fixed specimens after up to 30\u00a0days under vacuum storage gave equivalent results compared to standard histopathological slides and molecular tests, regarding both hematoxylin-eosin, immuno-stained slides and also nucleic acid extracted for molecular tests. The proposal of under-vacuum sealing pathology specimens that were previously formalin fixed can be adopted to transfer liquid-free biopsy and surgical specimens to referral pathology services. In fact, it is easy to perform, less expensive (both plastic bags and domestic-type vacuum chamber machines are at affordable costs), and above all is fully safe and adequate in the pre-analytical processing of pathology specimens.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00428-018-2383-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1106235", 
        "issn": [
          "0945-6317", 
          "1432-2307"
        ], 
        "name": "Virchows Archiv", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "473"
      }
    ], 
    "name": "Safe transportation of formalin-fixed liquid-free pathology specimens", 
    "pagination": "105-113", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ac80608a5a5099092e21634c2af694b4a0573d644fe8d2efd7dac09f2a6c3412"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29860620"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9423843"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00428-018-2383-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1104369766"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00428-018-2383-4", 
      "https://app.dimensions.ai/details/publication/pub.1104369766"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000604.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00428-018-2383-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00428-018-2383-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00428-018-2383-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00428-018-2383-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00428-018-2383-4'


 

This table displays all metadata directly associated to this object as RDF triples.

237 TRIPLES      21 PREDICATES      61 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00428-018-2383-4 schema:about N33fdbb1f187447e68985370ca17d578f
2 N364000a7152f4e6aa87532daf991145d
3 N6915cf928e294f54aa56f1cba2d75a99
4 N8b8d6ace71c544c3960aceb0db31b787
5 Na6445563312649b29cb5050e5526812f
6 Na8c8f5c799e74823a14ffca36d90b068
7 Nc6878e84b6b24a18b8a1bc79944237ee
8 Nd516470c08da43a59e3fcc990701f598
9 Ndc37893a659e4f23859a09a2d95d9535
10 Nef007e04f7c845b3a65fdc090022e5f2
11 anzsrc-for:11
12 anzsrc-for:1103
13 schema:author Nf03592516bc04546a414a554f4f20afa
14 schema:citation sg:pub.10.1007/978-1-61779-286-1_8
15 sg:pub.10.1007/978-3-319-13957-9_2
16 sg:pub.10.1007/s00428-006-0233-2
17 sg:pub.10.1007/s00428-007-0529-x
18 sg:pub.10.1038/bjc.2017.170
19 sg:pub.10.1038/modpathol.2009.117
20 sg:pub.10.1038/sj.bjc.6602889
21 https://app.dimensions.ai/details/publication/pub.1075053673
22 https://doi.org/10.1002/path.2574
23 https://doi.org/10.1016/j.mrrev.2011.07.003
24 https://doi.org/10.1016/j.scitotenv.2010.04.022
25 https://doi.org/10.1016/s1470-2045(12)70598-3
26 https://doi.org/10.1016/s1470-2045(13)70109-8
27 https://doi.org/10.1043/1543-2165-132.12.1929
28 https://doi.org/10.1043/2008-0432-ccr.1
29 https://doi.org/10.1080/08923973.2016.1248844
30 https://doi.org/10.1080/10520290701375302
31 https://doi.org/10.1101/pdb.prot4986
32 https://doi.org/10.1158/1055-9965.epi-09-0762
33 https://doi.org/10.1177/33.8.3894502
34 https://doi.org/10.1371/journal.pone.0168466
35 https://doi.org/10.1371/journal.pone.0182965
36 schema:datePublished 2018-07
37 schema:datePublishedReg 2018-07-01
38 schema:description Diagnostic pathology activities are largely based on fixation of tissues in 4% formaldehyde, which has recently been re-classified as a carcinogenic compound and banned in several countries. Hospitals that do not have in-house pathology services need to send surgical and biopsy specimens to referral centers. These are generally transferred in liquid containers, under suboptimal safety conditions, as accidental spillage of potentially dangerous substances may occur. A safe, innovative, two-step procedure for pathology sample transportation is presented. Formalin-fixed material from ten surgical cases was dissected (including surrogate biopsies) and preserved in liquid-free plastic bags under vacuum for up to 30 days and subsequently processed for conventional histology, several immunohistochemical markers, and molecular tests (e.g., RAS mutation). The data were compared with the corresponding routine analyses. Formalin-fixed specimens after up to 30 days under vacuum storage gave equivalent results compared to standard histopathological slides and molecular tests, regarding both hematoxylin-eosin, immuno-stained slides and also nucleic acid extracted for molecular tests. The proposal of under-vacuum sealing pathology specimens that were previously formalin fixed can be adopted to transfer liquid-free biopsy and surgical specimens to referral pathology services. In fact, it is easy to perform, less expensive (both plastic bags and domestic-type vacuum chamber machines are at affordable costs), and above all is fully safe and adequate in the pre-analytical processing of pathology specimens.
39 schema:genre research_article
40 schema:inLanguage en
41 schema:isAccessibleForFree false
42 schema:isPartOf N24b1c27d85ac4c8daaa6037d683ef2a7
43 Nd9c338cd8107462c8f562e6f3600db97
44 sg:journal.1106235
45 schema:name Safe transportation of formalin-fixed liquid-free pathology specimens
46 schema:pagination 105-113
47 schema:productId N6ab6a0afc4bc45f1921a0d1f937341bf
48 Naf28170ab311432ca69bef1c9c3af16c
49 Nbca7e3208de041ed9fe288af3af4f4b4
50 Nd9c6db235d2b4dc2b0c3a6366be6094f
51 Ne7c46c1c552048b7b2cf1298e1777659
52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104369766
53 https://doi.org/10.1007/s00428-018-2383-4
54 schema:sdDatePublished 2019-04-10T19:21
55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
56 schema:sdPublisher N086685cf1a0046f097ecebb1750d97f4
57 schema:url http://link.springer.com/10.1007%2Fs00428-018-2383-4
58 sgo:license sg:explorer/license/
59 sgo:sdDataset articles
60 rdf:type schema:ScholarlyArticle
61 N03f2fc9b26894a1caf3315e93a096a1e rdf:first sg:person.01141770554.98
62 rdf:rest N196398934e844e8ca6128e56394bb7a0
63 N086685cf1a0046f097ecebb1750d97f4 schema:name Springer Nature - SN SciGraph project
64 rdf:type schema:Organization
65 N129e921c0a344f50ab1b5fc8e17a2f43 rdf:first Nacd4e04501da43908c783c0011ddcee7
66 rdf:rest Naee669d260004626bd663ef27e504b7a
67 N130b075e438a4977bd3168eeb9c6a8ea rdf:first N9ccda16d35134abf8c303d28bb50f795
68 rdf:rest Ne7f3c7e3b07948ab94d93cee212771b3
69 N196398934e844e8ca6128e56394bb7a0 rdf:first sg:person.012315325032.08
70 rdf:rest N129e921c0a344f50ab1b5fc8e17a2f43
71 N24b1c27d85ac4c8daaa6037d683ef2a7 schema:issueNumber 1
72 rdf:type schema:PublicationIssue
73 N33fdbb1f187447e68985370ca17d578f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Paraffin Embedding
75 rdf:type schema:DefinedTerm
76 N364000a7152f4e6aa87532daf991145d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Specimen Handling
78 rdf:type schema:DefinedTerm
79 N6915cf928e294f54aa56f1cba2d75a99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Vacuum
81 rdf:type schema:DefinedTerm
82 N6ab6a0afc4bc45f1921a0d1f937341bf schema:name readcube_id
83 schema:value ac80608a5a5099092e21634c2af694b4a0573d644fe8d2efd7dac09f2a6c3412
84 rdf:type schema:PropertyValue
85 N6d28c202c4524c3fb439f14382bfe921 rdf:first sg:person.01215602014.60
86 rdf:rest N130b075e438a4977bd3168eeb9c6a8ea
87 N8b8d6ace71c544c3960aceb0db31b787 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Formaldehyde
89 rdf:type schema:DefinedTerm
90 N9ccda16d35134abf8c303d28bb50f795 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
91 schema:familyName Bussolati
92 schema:givenName G.
93 rdf:type schema:Person
94 Na6445563312649b29cb5050e5526812f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Transportation
96 rdf:type schema:DefinedTerm
97 Na8c8f5c799e74823a14ffca36d90b068 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Tissue Fixation
99 rdf:type schema:DefinedTerm
100 Nacd4e04501da43908c783c0011ddcee7 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
101 schema:familyName Veneziano
102 schema:givenName F.
103 rdf:type schema:Person
104 Naee669d260004626bd663ef27e504b7a rdf:first sg:person.01340627506.99
105 rdf:rest Nd9b8d953623f4603a110bdca9ee0b2e6
106 Naf28170ab311432ca69bef1c9c3af16c schema:name doi
107 schema:value 10.1007/s00428-018-2383-4
108 rdf:type schema:PropertyValue
109 Nbca7e3208de041ed9fe288af3af4f4b4 schema:name nlm_unique_id
110 schema:value 9423843
111 rdf:type schema:PropertyValue
112 Nc6878e84b6b24a18b8a1bc79944237ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Biopsy
114 rdf:type schema:DefinedTerm
115 Nd516470c08da43a59e3fcc990701f598 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Hematoxylin
117 rdf:type schema:DefinedTerm
118 Nd9b8d953623f4603a110bdca9ee0b2e6 rdf:first sg:person.01266642530.20
119 rdf:rest N6d28c202c4524c3fb439f14382bfe921
120 Nd9c338cd8107462c8f562e6f3600db97 schema:volumeNumber 473
121 rdf:type schema:PublicationVolume
122 Nd9c6db235d2b4dc2b0c3a6366be6094f schema:name pubmed_id
123 schema:value 29860620
124 rdf:type schema:PropertyValue
125 Ndc37893a659e4f23859a09a2d95d9535 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Humans
127 rdf:type schema:DefinedTerm
128 Ne7c46c1c552048b7b2cf1298e1777659 schema:name dimensions_id
129 schema:value pub.1104369766
130 rdf:type schema:PropertyValue
131 Ne7f3c7e3b07948ab94d93cee212771b3 rdf:first sg:person.0633774363.72
132 rdf:rest rdf:nil
133 Nef007e04f7c845b3a65fdc090022e5f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Tissue Preservation
135 rdf:type schema:DefinedTerm
136 Nf03592516bc04546a414a554f4f20afa rdf:first sg:person.015742566052.87
137 rdf:rest N03f2fc9b26894a1caf3315e93a096a1e
138 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
139 schema:name Medical and Health Sciences
140 rdf:type schema:DefinedTerm
141 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
142 schema:name Clinical Sciences
143 rdf:type schema:DefinedTerm
144 sg:journal.1106235 schema:issn 0945-6317
145 1432-2307
146 schema:name Virchows Archiv
147 rdf:type schema:Periodical
148 sg:person.01141770554.98 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
149 schema:familyName Bertero
150 schema:givenName L.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141770554.98
152 rdf:type schema:Person
153 sg:person.01215602014.60 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
154 schema:familyName Cassoni
155 schema:givenName P.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215602014.60
157 rdf:type schema:Person
158 sg:person.012315325032.08 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
159 schema:familyName Musuraca
160 schema:givenName C.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012315325032.08
162 rdf:type schema:Person
163 sg:person.01266642530.20 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
164 schema:familyName Mariani
165 schema:givenName S.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266642530.20
167 rdf:type schema:Person
168 sg:person.01340627506.99 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
169 schema:familyName Annaratone
170 schema:givenName L.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340627506.99
172 rdf:type schema:Person
173 sg:person.015742566052.87 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
174 schema:familyName Metovic
175 schema:givenName J.
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015742566052.87
177 rdf:type schema:Person
178 sg:person.0633774363.72 schema:affiliation https://www.grid.ac/institutes/grid.7605.4
179 schema:familyName Papotti
180 schema:givenName Mauro
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633774363.72
182 rdf:type schema:Person
183 sg:pub.10.1007/978-1-61779-286-1_8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041056695
184 https://doi.org/10.1007/978-1-61779-286-1_8
185 rdf:type schema:CreativeWork
186 sg:pub.10.1007/978-3-319-13957-9_2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018049697
187 https://doi.org/10.1007/978-3-319-13957-9_2
188 rdf:type schema:CreativeWork
189 sg:pub.10.1007/s00428-006-0233-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009364868
190 https://doi.org/10.1007/s00428-006-0233-2
191 rdf:type schema:CreativeWork
192 sg:pub.10.1007/s00428-007-0529-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013120467
193 https://doi.org/10.1007/s00428-007-0529-x
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/bjc.2017.170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086031089
196 https://doi.org/10.1038/bjc.2017.170
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/modpathol.2009.117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021192460
199 https://doi.org/10.1038/modpathol.2009.117
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/sj.bjc.6602889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049332317
202 https://doi.org/10.1038/sj.bjc.6602889
203 rdf:type schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1075053673 schema:CreativeWork
205 https://doi.org/10.1002/path.2574 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038282602
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.mrrev.2011.07.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009037272
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.scitotenv.2010.04.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013147790
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s1470-2045(12)70598-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009894380
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/s1470-2045(13)70109-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036459827
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1043/1543-2165-132.12.1929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077819148
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1043/2008-0432-ccr.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078340665
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1080/08923973.2016.1248844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036364750
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1080/10520290701375302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038961766
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1101/pdb.prot4986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060410923
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1158/1055-9965.epi-09-0762 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028958087
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1177/33.8.3894502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016604505
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1371/journal.pone.0168466 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021753001
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1371/journal.pone.0182965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091143093
232 rdf:type schema:CreativeWork
233 https://www.grid.ac/institutes/grid.7605.4 schema:alternateName University of Turin
234 schema:name Anatomia Patologica, Università di Torino, Via Santena 7, 10126, Torino, Italy
235 Pathology Unit, Department of Medical Sciences, University of Turin and Città della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy
236 Pathology Unit, Department of Oncology, University of Turin and Città della Salute e della Scienza Hospital, Via Santena 7, 10126, Turin, Italy
237 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...