Substratum adhesion and gliding in a diatom are mediated by extracellular proteoglycans View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-09

AUTHORS

Jan L. Lind, Kirsten Heimann, Elizabeth A. Miller, Catherine van Vliet, Nicholas J. Hoogenraad, Richard Wetherbee

ABSTRACT

. Diatoms are unicellular microalgae encased in a siliceous cell wall, or frustule. Pennate diatoms, which possess bilateral symmetry, attach to the substratum at a slit in the frustule called the raphe. These diatoms not only adhere, but glide across surfaces whilst maintaining their attachment, secreting a sticky mucilage that forms a trail behind the gliding cells. We have raised monoclonal antibodies to the major cell surface proteoglycans of the marine raphid diatom Stauroneis decipiens Hustedt. The antibody StF.H4 binds to the cell surface, in the raphe and to adhesive trails and inhibits the ability of living diatoms to adhere to the substratum and to glide. Moreover, StF.H4 binds to a periodate-insensitive epitope on four frustule-associated proteoglycans (relative molecular masses 87, 112, and >200 kDa). Another monoclonal antibody, StF.D5, binds to a carbohydrate epitope on the same set of proteoglycans, although the antibody binds only to the outer surface of the frustule and does not inhibit cell motility and adhesion. More... »

PAGES

213-221

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004250050184

DOI

http://dx.doi.org/10.1007/s004250050184

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026698255

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9362567


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Polarity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diatoms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred BALB C", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Immunoelectron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteoglycans", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lind", 
        "givenName": "Jan L.", 
        "id": "sg:person.01263633521.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263633521.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heimann", 
        "givenName": "Kirsten", 
        "id": "sg:person.0665135315.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665135315.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "Elizabeth A.", 
        "id": "sg:person.0627102647.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Vliet", 
        "givenName": "Catherine", 
        "id": "sg:person.01045611421.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045611421.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, La Trobe University, Bundoora, 3083, Australia, AU", 
          "id": "http://www.grid.ac/institutes/grid.1018.8", 
          "name": [
            "Department of Biochemistry, La Trobe University, Bundoora, 3083, Australia, AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hoogenraad", 
        "givenName": "Nicholas J.", 
        "id": "sg:person.0617517702.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617517702.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, University of Melbourne, Parkville, 3052, Australia, AU"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wetherbee", 
        "givenName": "Richard", 
        "id": "sg:person.01224222341.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224222341.91"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1997-09", 
    "datePublishedReg": "1997-09-01", 
    "description": "Abstract. Diatoms are unicellular microalgae encased in a siliceous cell wall, or frustule. Pennate diatoms, which possess bilateral symmetry, attach to the substratum at a slit in the frustule called the raphe. These diatoms not only adhere, but glide across surfaces whilst maintaining their attachment, secreting a sticky mucilage that forms a trail behind the gliding cells. We have raised monoclonal antibodies to the major cell surface proteoglycans of the marine raphid diatom Stauroneis decipiens Hustedt. The antibody StF.H4 binds to the cell surface, in the raphe and to adhesive trails and inhibits the ability of living diatoms to adhere to the substratum and to glide. Moreover, StF.H4 binds to a periodate-insensitive epitope on four frustule-associated proteoglycans (relative molecular masses 87, 112, and >200 kDa). Another monoclonal antibody, StF.D5, binds to a carbohydrate epitope on the same set of proteoglycans, although the antibody binds only to the outer surface of the frustule and does not inhibit cell motility and adhesion.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s004250050184", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "203"
      }
    ], 
    "keywords": [
      "H4 binds", 
      "siliceous cell wall", 
      "periodate-insensitive epitopes", 
      "cell surface proteoglycans", 
      "sticky mucilage", 
      "unicellular microalgae", 
      "pennate diatoms", 
      "cell motility", 
      "cell wall", 
      "substratum adhesion", 
      "surface proteoglycans", 
      "extracellular proteoglycans", 
      "diatoms", 
      "cell surface", 
      "monoclonal antibodies", 
      "bilateral symmetry", 
      "proteoglycans", 
      "frustules", 
      "binds", 
      "carbohydrate epitopes", 
      "substratum", 
      "gliding cells", 
      "adhesion", 
      "Hustedt", 
      "microalgae", 
      "epitopes", 
      "mucilage", 
      "motility", 
      "antibodies", 
      "cells", 
      "trails", 
      "same set", 
      "outer surface", 
      "attachment", 
      "ability", 
      "wall", 
      "D5", 
      "raphe", 
      "surface", 
      "set", 
      "slit", 
      "symmetry"
    ], 
    "name": "Substratum adhesion and gliding in a diatom are mediated by extracellular proteoglycans", 
    "pagination": "213-221", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026698255"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s004250050184"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9362567"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s004250050184", 
      "https://app.dimensions.ai/details/publication/pub.1026698255"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:30", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_287.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s004250050184"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s004250050184'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s004250050184'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s004250050184'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s004250050184'


 

This table displays all metadata directly associated to this object as RDF triples.

181 TRIPLES      20 PREDICATES      78 URIs      70 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s004250050184 schema:about N021a7a60043d439d9200d0ae28368419
2 N07451344e320413a80aac80ea2022a94
3 N3ca06a71306c413582936eaf072c675c
4 N742f80f787694b07866bdc811a2be8e7
5 N83371f31e5034ba6b9fc90bea2354c04
6 N86ed40595a594756917ad237c43dbdf8
7 N90f9b2ecd7cc4a62991f8721c4ec9f9a
8 Nb95f0d469d2d4eb58b7b1d492b969bba
9 Nd5c724b6fba84e5e89206b0cb9f71d56
10 Nf8810e796b87431abd788f75dd0eee5f
11 anzsrc-for:06
12 anzsrc-for:0601
13 schema:author N5b703ee4ee16478cbf0f19dc3679837e
14 schema:datePublished 1997-09
15 schema:datePublishedReg 1997-09-01
16 schema:description Abstract. Diatoms are unicellular microalgae encased in a siliceous cell wall, or frustule. Pennate diatoms, which possess bilateral symmetry, attach to the substratum at a slit in the frustule called the raphe. These diatoms not only adhere, but glide across surfaces whilst maintaining their attachment, secreting a sticky mucilage that forms a trail behind the gliding cells. We have raised monoclonal antibodies to the major cell surface proteoglycans of the marine raphid diatom Stauroneis decipiens Hustedt. The antibody StF.H4 binds to the cell surface, in the raphe and to adhesive trails and inhibits the ability of living diatoms to adhere to the substratum and to glide. Moreover, StF.H4 binds to a periodate-insensitive epitope on four frustule-associated proteoglycans (relative molecular masses 87, 112, and >200 kDa). Another monoclonal antibody, StF.D5, binds to a carbohydrate epitope on the same set of proteoglycans, although the antibody binds only to the outer surface of the frustule and does not inhibit cell motility and adhesion.
17 schema:genre article
18 schema:isAccessibleForFree false
19 schema:isPartOf N010faff31c7544f98a5687ff67a483d8
20 N066ed808b11d4c3f890813c7bfbbbc76
21 sg:journal.1054035
22 schema:keywords D5
23 H4 binds
24 Hustedt
25 ability
26 adhesion
27 antibodies
28 attachment
29 bilateral symmetry
30 binds
31 carbohydrate epitopes
32 cell motility
33 cell surface
34 cell surface proteoglycans
35 cell wall
36 cells
37 diatoms
38 epitopes
39 extracellular proteoglycans
40 frustules
41 gliding cells
42 microalgae
43 monoclonal antibodies
44 motility
45 mucilage
46 outer surface
47 pennate diatoms
48 periodate-insensitive epitopes
49 proteoglycans
50 raphe
51 same set
52 set
53 siliceous cell wall
54 slit
55 sticky mucilage
56 substratum
57 substratum adhesion
58 surface
59 surface proteoglycans
60 symmetry
61 trails
62 unicellular microalgae
63 wall
64 schema:name Substratum adhesion and gliding in a diatom are mediated by extracellular proteoglycans
65 schema:pagination 213-221
66 schema:productId N093ae4bd4fa340259110546d05a87c58
67 N0b63da416fb840118412a98a05c388de
68 Ne7b0a1d45fc942eba44b8a296e6a880e
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026698255
70 https://doi.org/10.1007/s004250050184
71 schema:sdDatePublished 2022-10-01T06:30
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N399e4f81d1004677a46333cfac9635fc
74 schema:url https://doi.org/10.1007/s004250050184
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N010faff31c7544f98a5687ff67a483d8 schema:volumeNumber 203
79 rdf:type schema:PublicationVolume
80 N011e914cb5224bc2ba8ce3e61a9390f5 rdf:first sg:person.0665135315.32
81 rdf:rest N55938c0b9a3d4c3db91b061f2c6d46f5
82 N021a7a60043d439d9200d0ae28368419 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Microscopy, Immunoelectron
84 rdf:type schema:DefinedTerm
85 N066ed808b11d4c3f890813c7bfbbbc76 schema:issueNumber 2
86 rdf:type schema:PublicationIssue
87 N07451344e320413a80aac80ea2022a94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Antibodies, Monoclonal
89 rdf:type schema:DefinedTerm
90 N093ae4bd4fa340259110546d05a87c58 schema:name doi
91 schema:value 10.1007/s004250050184
92 rdf:type schema:PropertyValue
93 N0b63da416fb840118412a98a05c388de schema:name pubmed_id
94 schema:value 9362567
95 rdf:type schema:PropertyValue
96 N399e4f81d1004677a46333cfac9635fc schema:name Springer Nature - SN SciGraph project
97 rdf:type schema:Organization
98 N3ca06a71306c413582936eaf072c675c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Proteoglycans
100 rdf:type schema:DefinedTerm
101 N528b34b0d075430c93b99870b868233f rdf:first sg:person.0617517702.57
102 rdf:rest N7333adb2827c437cad14d8f8da9bc033
103 N55938c0b9a3d4c3db91b061f2c6d46f5 rdf:first sg:person.0627102647.36
104 rdf:rest N8a41814f9e7f41238949165b6350f3e9
105 N5b703ee4ee16478cbf0f19dc3679837e rdf:first sg:person.01263633521.09
106 rdf:rest N011e914cb5224bc2ba8ce3e61a9390f5
107 N7333adb2827c437cad14d8f8da9bc033 rdf:first sg:person.01224222341.91
108 rdf:rest rdf:nil
109 N742f80f787694b07866bdc811a2be8e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Mice, Inbred BALB C
111 rdf:type schema:DefinedTerm
112 N83371f31e5034ba6b9fc90bea2354c04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Cell Adhesion
114 rdf:type schema:DefinedTerm
115 N86ed40595a594756917ad237c43dbdf8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Mice
117 rdf:type schema:DefinedTerm
118 N8a41814f9e7f41238949165b6350f3e9 rdf:first sg:person.01045611421.17
119 rdf:rest N528b34b0d075430c93b99870b868233f
120 N90f9b2ecd7cc4a62991f8721c4ec9f9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Cell Movement
122 rdf:type schema:DefinedTerm
123 Nb95f0d469d2d4eb58b7b1d492b969bba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Cell Polarity
125 rdf:type schema:DefinedTerm
126 Nd5c724b6fba84e5e89206b0cb9f71d56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Animals
128 rdf:type schema:DefinedTerm
129 Ne7b0a1d45fc942eba44b8a296e6a880e schema:name dimensions_id
130 schema:value pub.1026698255
131 rdf:type schema:PropertyValue
132 Nf8810e796b87431abd788f75dd0eee5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Diatoms
134 rdf:type schema:DefinedTerm
135 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
136 schema:name Biological Sciences
137 rdf:type schema:DefinedTerm
138 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biochemistry and Cell Biology
140 rdf:type schema:DefinedTerm
141 sg:journal.1054035 schema:issn 0032-0935
142 1432-2048
143 schema:name Planta
144 schema:publisher Springer Nature
145 rdf:type schema:Periodical
146 sg:person.01045611421.17 schema:affiliation grid-institutes:grid.1008.9
147 schema:familyName van Vliet
148 schema:givenName Catherine
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045611421.17
150 rdf:type schema:Person
151 sg:person.01224222341.91 schema:affiliation grid-institutes:grid.1008.9
152 schema:familyName Wetherbee
153 schema:givenName Richard
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224222341.91
155 rdf:type schema:Person
156 sg:person.01263633521.09 schema:affiliation grid-institutes:grid.1008.9
157 schema:familyName Lind
158 schema:givenName Jan L.
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263633521.09
160 rdf:type schema:Person
161 sg:person.0617517702.57 schema:affiliation grid-institutes:grid.1018.8
162 schema:familyName Hoogenraad
163 schema:givenName Nicholas J.
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617517702.57
165 rdf:type schema:Person
166 sg:person.0627102647.36 schema:affiliation grid-institutes:grid.1008.9
167 schema:familyName Miller
168 schema:givenName Elizabeth A.
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36
170 rdf:type schema:Person
171 sg:person.0665135315.32 schema:affiliation grid-institutes:grid.1008.9
172 schema:familyName Heimann
173 schema:givenName Kirsten
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665135315.32
175 rdf:type schema:Person
176 grid-institutes:grid.1008.9 schema:alternateName School of Botany, University of Melbourne, Parkville, 3052, Australia, AU
177 schema:name School of Botany, University of Melbourne, Parkville, 3052, Australia, AU
178 rdf:type schema:Organization
179 grid-institutes:grid.1018.8 schema:alternateName Department of Biochemistry, La Trobe University, Bundoora, 3083, Australia, AU
180 schema:name Department of Biochemistry, La Trobe University, Bundoora, 3083, Australia, AU
181 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...