Identification and characterisation of a glycine-rich RNA-binding protein as an endogenous suppressor of RNA silencing from Nicotiana glutinosa View Full Text


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Article Info

DATE

2019-03-06

AUTHORS

Xu Huang, Ru Yu, Wenjing Li, Liwei Geng, Xiuli Jing, Changxiang Zhu, Hongmei Liu

ABSTRACT

Main conclusionThis study shows that NgRBP suppresses both local and systemic RNA silencing induced by sense- or double-stranded RNA, and the RNA binding activity is essential for its function.To counteract host defence, many plant viruses encode viral suppressors of RNA silencing targeting various stages of RNA silencing. There is increasing evidence that the plants also encode endogenous suppressors of RNA silencing (ESR) to regulate this pathway. In this study, using Agrobacterium infiltration assays, we characterized NgRBP, a glycine-rich RNA-binding protein from Nicotiana glutinosa, as an ESR. Our results indicated that NgRBP suppressed both local and systemic RNA silencing induced by sense- or double-stranded RNA. We also demonstrated that NgRBP could promote Potato Virus X (PVX) infection in N. benthamiana. NgRBP knockdown by virus-induced gene silencing enhanced PVX and Cucumber mosaic virus resistance in N. glutinosa. RNA immunoprecipitation and electrophoretic mobility shift assays showed that NgRBP bound to GFP mRNA, dsRNA rather than siRNA. These findings provide the evidence that NgRBP acts as an ESR and the RNA affinity of NgRBP plays the key role in its ESR activity. NgRBP responds to multiple signals such as ABA, MeJA, SA, and Tobacco mosaic virus infection. Therefore, it could participate in the regulation of gene expression under specific conditions. More... »

PAGES

1811-1822

References to SciGraph publications

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    http://scigraph.springernature.com/pub.10.1007/s00425-019-03122-5

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    38 schema:description Main conclusionThis study shows that NgRBP suppresses both local and systemic RNA silencing induced by sense- or double-stranded RNA, and the RNA binding activity is essential for its function.To counteract host defence, many plant viruses encode viral suppressors of RNA silencing targeting various stages of RNA silencing. There is increasing evidence that the plants also encode endogenous suppressors of RNA silencing (ESR) to regulate this pathway. In this study, using Agrobacterium infiltration assays, we characterized NgRBP, a glycine-rich RNA-binding protein from Nicotiana glutinosa, as an ESR. Our results indicated that NgRBP suppressed both local and systemic RNA silencing induced by sense- or double-stranded RNA. We also demonstrated that NgRBP could promote Potato Virus X (PVX) infection in N. benthamiana. NgRBP knockdown by virus-induced gene silencing enhanced PVX and Cucumber mosaic virus resistance in N. glutinosa. RNA immunoprecipitation and electrophoretic mobility shift assays showed that NgRBP bound to GFP mRNA, dsRNA rather than siRNA. These findings provide the evidence that NgRBP acts as an ESR and the RNA affinity of NgRBP plays the key role in its ESR activity. NgRBP responds to multiple signals such as ABA, MeJA, SA, and Tobacco mosaic virus infection. Therefore, it could participate in the regulation of gene expression under specific conditions.
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    46 Agrobacterium infiltration assays
    47 ConclusionThis study
    48 ESR
    49 ESR activity
    50 GFP mRNA
    51 Main conclusionThis study
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    53 NgRBP
    54 NgRBP knockdown
    55 Nicotiana glutinosa
    56 PVX
    57 RNA
    58 RNA affinity
    59 RNA binding activity
    60 RNA immunoprecipitation
    61 RNA-binding protein
    62 SA
    63 Virus X (PVX) infection
    64 X (PVX) infection
    65 activity
    66 affinity
    67 assays
    68 benthamiana
    69 binding activity
    70 characterisation
    71 conditions
    72 cucumber mosaic virus resistance
    73 defense
    74 dsRNA
    75 electrophoretic mobility shift assays
    76 endogenous suppressor
    77 evidence
    78 expression
    79 findings
    80 function
    81 gene expression
    82 genes
    83 glutinosa
    84 glycine-rich RNA-binding protein
    85 host defense
    86 identification
    87 immunoprecipitation
    88 infection
    89 infiltration assays
    90 key role
    91 knockdown
    92 mRNA
    93 mobility shift assays
    94 mosaic virus infection
    95 mosaic virus resistance
    96 multiple signals
    97 pathway
    98 plant viruses
    99 plants
    100 potato virus X infection
    101 protein
    102 regulation
    103 resistance
    104 results
    105 role
    106 sense
    107 shift assays
    108 siRNA
    109 signals
    110 specific conditions
    111 stage
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    115 systemic RNA
    116 tobacco mosaic virus infection
    117 viral suppressors
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