Medicago glucosyltransferase UGT72L1: potential roles in proanthocyanidin biosynthesis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-04-17

AUTHORS

Yongzhen Pang, Xiaofei Cheng, David V. Huhman, Junying Ma, Gregory J. Peel, Keiko Yonekura-Sakakibara, Kazuki Saito, Guoan Shen, Lloyd W. Sumner, Yuhong Tang, Jianqi Wen, Jianfei Yun, Richard A. Dixon

ABSTRACT

In the first reaction specific for proanthocyanidin (PA) biosynthesis in Arabidopsis thaliana and Medicago truncatula, anthocyanidin reductase (ANR) converts cyanidin to (−)-epicatechin. The glucosyltransferase UGT72L1 catalyzes formation of epicatechin 3′-O-glucoside (E3′OG), the preferred substrate for MATE transporters implicated in PA biosynthesis in both species. The mechanism of PA polymerization is still unclear, but may involve the laccase-like polyphenol oxidase TRANSPARENT TESTA 10 (TT10). We have employed a combination of cell biological, biochemical and genetic approaches to evaluate this PA pathway model. The promoter regions of UGT72L1 and MtANR share common cis-acting elements and direct overlapping, but partially distinct, expression patterns. UGT72L1 and MtANR are localized in the cytosol, whereas TT10 is localized to the vacuole. Over-expression of UGT72L1 in M. truncatula hairy roots results in increased accumulation of PA-like compounds, and loss of function of UGT72L1 partially reduces epicatechin, E3′OG and extractable PA levels in M. truncatula seeds. Expression of UGT72L1 in A. thaliana leads to a massive increase in E3′OG in immature seed, but reduced levels of extractable PAs. However, when UGT72L1 was expressed in the Arabidopsis tt10 mutant, extractable PA levels increased and seed coat browning was delayed. Our results suggest that glycosylation of epicatechin is important for both PA precursor transport and assembly, but that additional redundant pathways may exist. More... »

PAGES

139-154

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00425-013-1879-z

DOI

http://dx.doi.org/10.1007/s00425-013-1879-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002265029

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23592226


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26 schema:description In the first reaction specific for proanthocyanidin (PA) biosynthesis in Arabidopsis thaliana and Medicago truncatula, anthocyanidin reductase (ANR) converts cyanidin to (−)-epicatechin. The glucosyltransferase UGT72L1 catalyzes formation of epicatechin 3′-O-glucoside (E3′OG), the preferred substrate for MATE transporters implicated in PA biosynthesis in both species. The mechanism of PA polymerization is still unclear, but may involve the laccase-like polyphenol oxidase TRANSPARENT TESTA 10 (TT10). We have employed a combination of cell biological, biochemical and genetic approaches to evaluate this PA pathway model. The promoter regions of UGT72L1 and MtANR share common cis-acting elements and direct overlapping, but partially distinct, expression patterns. UGT72L1 and MtANR are localized in the cytosol, whereas TT10 is localized to the vacuole. Over-expression of UGT72L1 in M. truncatula hairy roots results in increased accumulation of PA-like compounds, and loss of function of UGT72L1 partially reduces epicatechin, E3′OG and extractable PA levels in M. truncatula seeds. Expression of UGT72L1 in A. thaliana leads to a massive increase in E3′OG in immature seed, but reduced levels of extractable PAs. However, when UGT72L1 was expressed in the Arabidopsis tt10 mutant, extractable PA levels increased and seed coat browning was delayed. Our results suggest that glycosylation of epicatechin is important for both PA precursor transport and assembly, but that additional redundant pathways may exist.
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33 schema:keywords Arabidopsis thaliana
34 Arabidopsis tt10 mutant
35 E3′OG
36 MATE transporters
37 Medicago glucosyltransferase
38 Medicago truncatula
39 MtANR
40 PA
41 PA biosynthesis
42 PA levels
43 PA pathway model
44 PA polymerization
45 PA precursor transport
46 PA-like compounds
47 TESTA 10
48 TRANSPARENT TESTA 10
49 TT10
50 UGT72L1
51 accumulation
52 additional redundant pathways
53 anthocyanidin reductase (ANR) converts
54 approach
55 assembly
56 biosynthesis
57 browning
58 cells
59 cis-acting elements
60 coat browning
61 combination
62 combination of cells
63 common cis-acting element
64 compounds
65 converts
66 cytosol
67 direct overlapping
68 elements
69 epicatechin
70 epicatechin-3
71 expression
72 expression of UGT72L1
73 expression patterns
74 extractable PA levels
75 extractable PAs
76 first reaction
77 formation
78 function
79 genetic approaches
80 glucoside
81 glucosyltransferase
82 glycosylation
83 glycosylation of epicatechin
84 immature seeds
85 increase
86 laccase-like polyphenol oxidase TRANSPARENT TESTA 10
87 levels
88 loss
89 loss of function
90 massive increase
91 mechanism
92 model
93 mutants
94 overlapping
95 oxidase TRANSPARENT TESTA 10
96 pathway
97 pathway model
98 patterns
99 polymerization
100 polyphenol oxidase TRANSPARENT TESTA 10
101 potential role
102 precursor transport
103 preferred substrate
104 proanthocyanidin biosynthesis
105 promoter region
106 reaction
107 reduced levels
108 reductase (ANR) converts
109 redundant pathways
110 region
111 results
112 role
113 seed coat browning
114 seeds
115 species
116 substrate
117 thaliana
118 transport
119 transporters
120 truncatula
121 truncatula seeds
122 tt10 mutant
123 vacuoles
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