Dual location of a family of proteinase inhibitors within the stigmas of Nicotiana alata View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-10-20

AUTHORS

Elizabeth D. Johnson, Elizabeth A. Miller, Marilyn A. Anderson

ABSTRACT

Reproductive and storage tissues of many plants produce large amounts of serine proteinase inhibitors (PIs). The ornamental tobacco, Nicotiana alata, produces a series of 6 kDa chymotrypsin and trypsin inhibitors that accumulate to up to 30% of soluble protein in the stigma. These inhibitors are derived by proteolytic processing of two closely related multidomain precursor proteins. Using immunogold electron microscopy, we find that the stigmatic PIs accumulate in both the central vacuole and in the extracellular mucilage. Labelling with antibodies specific for the C-terminal vacuolar targeting peptide (VTS) of each precursor confirms earlier biochemical data showing that the VTS is removed during passage through the secretory pathway. We have isolated and characterised the extracellular population of PIs, which are largely identical to PIs isolated from whole stigmas and are functional inhibitors of serine proteases. Subcellular fractionation of immature stigmas reveals that a sub-population of the PI precursor protein is proteolytically processed within the endoplasmic reticulum. This proteolysis results in the removal of the vacuolar sorting information, causing secretion of this PI population. We propose a novel mechanism whereby a single gene product may be simultaneously trafficked to two separate compartments mediated by proteolysis early in the secretory pathway. More... »

PAGES

1265-1276

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00425-006-0418-6

DOI

http://dx.doi.org/10.1007/s00425-006-0418-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034953674

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17053891


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chymotrypsin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flowers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protease Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trypsin Inhibitors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, La Trobe University, 3086, Melbourne, VIC, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1018.8", 
          "name": [
            "Department of Biochemistry, La Trobe University, 3086, Melbourne, VIC, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Johnson", 
        "givenName": "Elizabeth D.", 
        "id": "sg:person.01060341331.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060341331.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biological Sciences, Columbia University, New York, NY, USA", 
          "id": "http://www.grid.ac/institutes/grid.21729.3f", 
          "name": [
            "Department of Biological Sciences, Columbia University, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "Elizabeth A.", 
        "id": "sg:person.0627102647.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biochemistry, La Trobe University, 3086, Melbourne, VIC, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1018.8", 
          "name": [
            "Department of Biochemistry, La Trobe University, 3086, Melbourne, VIC, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Anderson", 
        "givenName": "Marilyn A.", 
        "id": "sg:person.01051153260.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051153260.90"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf01279880", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005030151", 
          "https://doi.org/10.1007/bf01279880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/33929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032225226", 
          "https://doi.org/10.1038/33929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/9293", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026619236", 
          "https://doi.org/10.1038/9293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006305429013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042836328", 
          "https://doi.org/10.1023/a:1006305429013"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-7091-6684-0_10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033271329", 
          "https://doi.org/10.1007/978-3-7091-6684-0_10"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-10-20", 
    "datePublishedReg": "2006-10-20", 
    "description": "Reproductive and storage tissues of many plants produce large amounts of serine proteinase inhibitors (PIs). The ornamental tobacco, Nicotiana alata, produces a series of 6\u00a0kDa chymotrypsin and trypsin inhibitors that accumulate to up to 30% of soluble protein in the stigma. These inhibitors are derived by proteolytic processing of two closely related multidomain precursor proteins. Using immunogold electron microscopy, we find that the stigmatic PIs accumulate in both the central vacuole and in the extracellular mucilage. Labelling with antibodies specific for the C-terminal vacuolar targeting peptide (VTS) of each precursor confirms earlier biochemical data showing that the VTS is removed during passage through the secretory pathway. We have isolated and characterised the extracellular population of PIs, which are largely identical to PIs isolated from whole stigmas and are functional inhibitors of serine proteases. Subcellular fractionation of immature stigmas reveals that a sub-population of the PI precursor protein is proteolytically processed within the endoplasmic reticulum. This proteolysis results in the removal of the vacuolar sorting information, causing secretion of this PI population. We propose a novel mechanism whereby a single gene product may be simultaneously trafficked to two separate compartments mediated by proteolysis early in the secretory pathway.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00425-006-0418-6", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "225"
      }
    ], 
    "keywords": [
      "proteinase inhibitors", 
      "secretory pathway", 
      "Nicotiana alata", 
      "earlier biochemical data", 
      "precursor protein", 
      "single gene product", 
      "vacuolar targeting peptide", 
      "serine proteinase inhibitor", 
      "ornamental tobacco", 
      "extracellular mucilage", 
      "gene products", 
      "central vacuole", 
      "subcellular fractionation", 
      "soluble protein", 
      "storage tissue", 
      "endoplasmic reticulum", 
      "dual location", 
      "proteolytic processing", 
      "serine proteases", 
      "novel mechanism", 
      "functional inhibitor", 
      "extracellular population", 
      "biochemical data", 
      "protein", 
      "immature stigmas", 
      "targeting peptide", 
      "trypsin inhibitor", 
      "proteolysis", 
      "alata", 
      "pathway", 
      "inhibitors", 
      "PI population", 
      "separate compartments", 
      "plants", 
      "reticulum", 
      "vacuoles", 
      "protease", 
      "mucilage", 
      "population", 
      "compartments", 
      "chymotrypsin", 
      "family", 
      "tobacco", 
      "peptides", 
      "tissue", 
      "secretion", 
      "large amount", 
      "mechanism", 
      "electron microscopy", 
      "fractionation", 
      "precursors", 
      "antibodies", 
      "microscopy", 
      "stigma", 
      "passage", 
      "products", 
      "location", 
      "amount", 
      "removal", 
      "data", 
      "information", 
      "processing", 
      "series", 
      "VTS"
    ], 
    "name": "Dual location of a family of proteinase inhibitors within the stigmas of Nicotiana alata", 
    "pagination": "1265-1276", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034953674"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00425-006-0418-6"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17053891"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00425-006-0418-6", 
      "https://app.dimensions.ai/details/publication/pub.1034953674"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:33", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00425-006-0418-6"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00425-006-0418-6'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00425-006-0418-6'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00425-006-0418-6'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00425-006-0418-6'


 

This table displays all metadata directly associated to this object as RDF triples.

198 TRIPLES      21 PREDICATES      103 URIs      89 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00425-006-0418-6 schema:about N081f5b894d77455ab51871c42fd22b87
2 N4b2ed812efee4305acb3478afabc6086
3 N556099cf48b449efae28764b5895fca2
4 Ncb41b761ec7647789a9dddcf83e74a0d
5 Nd44da03e92b84efd96bc9df3fb5c8a39
6 Ne23607e7fff647e38956164aa9e1d7fa
7 Ne275f2f4e38043b38b3cf8eb5ddfc046
8 Nfa892dedd0ad4dc5bf0379df45cad487
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author N0f39409a61c14937979060e2c153257f
12 schema:citation sg:pub.10.1007/978-3-7091-6684-0_10
13 sg:pub.10.1007/bf01279880
14 sg:pub.10.1023/a:1006305429013
15 sg:pub.10.1038/227680a0
16 sg:pub.10.1038/33929
17 sg:pub.10.1038/9293
18 schema:datePublished 2006-10-20
19 schema:datePublishedReg 2006-10-20
20 schema:description Reproductive and storage tissues of many plants produce large amounts of serine proteinase inhibitors (PIs). The ornamental tobacco, Nicotiana alata, produces a series of 6 kDa chymotrypsin and trypsin inhibitors that accumulate to up to 30% of soluble protein in the stigma. These inhibitors are derived by proteolytic processing of two closely related multidomain precursor proteins. Using immunogold electron microscopy, we find that the stigmatic PIs accumulate in both the central vacuole and in the extracellular mucilage. Labelling with antibodies specific for the C-terminal vacuolar targeting peptide (VTS) of each precursor confirms earlier biochemical data showing that the VTS is removed during passage through the secretory pathway. We have isolated and characterised the extracellular population of PIs, which are largely identical to PIs isolated from whole stigmas and are functional inhibitors of serine proteases. Subcellular fractionation of immature stigmas reveals that a sub-population of the PI precursor protein is proteolytically processed within the endoplasmic reticulum. This proteolysis results in the removal of the vacuolar sorting information, causing secretion of this PI population. We propose a novel mechanism whereby a single gene product may be simultaneously trafficked to two separate compartments mediated by proteolysis early in the secretory pathway.
21 schema:genre article
22 schema:isAccessibleForFree false
23 schema:isPartOf N0af2859d2a494d2985bd1c152cbf9faf
24 N24630cfcd26d4d04970e673fa242d28f
25 sg:journal.1054035
26 schema:keywords Nicotiana alata
27 PI population
28 VTS
29 alata
30 amount
31 antibodies
32 biochemical data
33 central vacuole
34 chymotrypsin
35 compartments
36 data
37 dual location
38 earlier biochemical data
39 electron microscopy
40 endoplasmic reticulum
41 extracellular mucilage
42 extracellular population
43 family
44 fractionation
45 functional inhibitor
46 gene products
47 immature stigmas
48 information
49 inhibitors
50 large amount
51 location
52 mechanism
53 microscopy
54 mucilage
55 novel mechanism
56 ornamental tobacco
57 passage
58 pathway
59 peptides
60 plants
61 population
62 precursor protein
63 precursors
64 processing
65 products
66 protease
67 protein
68 proteinase inhibitors
69 proteolysis
70 proteolytic processing
71 removal
72 reticulum
73 secretion
74 secretory pathway
75 separate compartments
76 series
77 serine proteases
78 serine proteinase inhibitor
79 single gene product
80 soluble protein
81 stigma
82 storage tissue
83 subcellular fractionation
84 targeting peptide
85 tissue
86 tobacco
87 trypsin inhibitor
88 vacuolar targeting peptide
89 vacuoles
90 schema:name Dual location of a family of proteinase inhibitors within the stigmas of Nicotiana alata
91 schema:pagination 1265-1276
92 schema:productId N9287b1ca2a0b47ea8f8fc9c5d41e79b9
93 N9ef5ef98d755470e8327a7207d50a81d
94 Nb228ffb305464aeebc7c4148d490f259
95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034953674
96 https://doi.org/10.1007/s00425-006-0418-6
97 schema:sdDatePublished 2022-10-01T06:33
98 schema:sdLicense https://scigraph.springernature.com/explorer/license/
99 schema:sdPublisher N4153df72037347c586526889df2f20bf
100 schema:url https://doi.org/10.1007/s00425-006-0418-6
101 sgo:license sg:explorer/license/
102 sgo:sdDataset articles
103 rdf:type schema:ScholarlyArticle
104 N081f5b894d77455ab51871c42fd22b87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Plant Proteins
106 rdf:type schema:DefinedTerm
107 N0af2859d2a494d2985bd1c152cbf9faf schema:issueNumber 5
108 rdf:type schema:PublicationIssue
109 N0f39409a61c14937979060e2c153257f rdf:first sg:person.01060341331.79
110 rdf:rest N5b344fb3eb824834b553832da4a2d169
111 N24630cfcd26d4d04970e673fa242d28f schema:volumeNumber 225
112 rdf:type schema:PublicationVolume
113 N4153df72037347c586526889df2f20bf schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 N4b2ed812efee4305acb3478afabc6086 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Chymotrypsin
117 rdf:type schema:DefinedTerm
118 N556099cf48b449efae28764b5895fca2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Tobacco
120 rdf:type schema:DefinedTerm
121 N5b344fb3eb824834b553832da4a2d169 rdf:first sg:person.0627102647.36
122 rdf:rest N86e9e2f2c83648e8838289b6c863ce77
123 N86e9e2f2c83648e8838289b6c863ce77 rdf:first sg:person.01051153260.90
124 rdf:rest rdf:nil
125 N9287b1ca2a0b47ea8f8fc9c5d41e79b9 schema:name doi
126 schema:value 10.1007/s00425-006-0418-6
127 rdf:type schema:PropertyValue
128 N9ef5ef98d755470e8327a7207d50a81d schema:name dimensions_id
129 schema:value pub.1034953674
130 rdf:type schema:PropertyValue
131 Nb228ffb305464aeebc7c4148d490f259 schema:name pubmed_id
132 schema:value 17053891
133 rdf:type schema:PropertyValue
134 Ncb41b761ec7647789a9dddcf83e74a0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Flowers
136 rdf:type schema:DefinedTerm
137 Nd44da03e92b84efd96bc9df3fb5c8a39 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Protease Inhibitors
139 rdf:type schema:DefinedTerm
140 Ne23607e7fff647e38956164aa9e1d7fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Microscopy, Electron
142 rdf:type schema:DefinedTerm
143 Ne275f2f4e38043b38b3cf8eb5ddfc046 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Trypsin Inhibitors
145 rdf:type schema:DefinedTerm
146 Nfa892dedd0ad4dc5bf0379df45cad487 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Kinetics
148 rdf:type schema:DefinedTerm
149 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
150 schema:name Biological Sciences
151 rdf:type schema:DefinedTerm
152 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biochemistry and Cell Biology
154 rdf:type schema:DefinedTerm
155 sg:journal.1054035 schema:issn 0032-0935
156 1432-2048
157 schema:name Planta
158 schema:publisher Springer Nature
159 rdf:type schema:Periodical
160 sg:person.01051153260.90 schema:affiliation grid-institutes:grid.1018.8
161 schema:familyName Anderson
162 schema:givenName Marilyn A.
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051153260.90
164 rdf:type schema:Person
165 sg:person.01060341331.79 schema:affiliation grid-institutes:grid.1018.8
166 schema:familyName Johnson
167 schema:givenName Elizabeth D.
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060341331.79
169 rdf:type schema:Person
170 sg:person.0627102647.36 schema:affiliation grid-institutes:grid.21729.3f
171 schema:familyName Miller
172 schema:givenName Elizabeth A.
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36
174 rdf:type schema:Person
175 sg:pub.10.1007/978-3-7091-6684-0_10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033271329
176 https://doi.org/10.1007/978-3-7091-6684-0_10
177 rdf:type schema:CreativeWork
178 sg:pub.10.1007/bf01279880 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005030151
179 https://doi.org/10.1007/bf01279880
180 rdf:type schema:CreativeWork
181 sg:pub.10.1023/a:1006305429013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042836328
182 https://doi.org/10.1023/a:1006305429013
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
185 https://doi.org/10.1038/227680a0
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/33929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032225226
188 https://doi.org/10.1038/33929
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/9293 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026619236
191 https://doi.org/10.1038/9293
192 rdf:type schema:CreativeWork
193 grid-institutes:grid.1018.8 schema:alternateName Department of Biochemistry, La Trobe University, 3086, Melbourne, VIC, Australia
194 schema:name Department of Biochemistry, La Trobe University, 3086, Melbourne, VIC, Australia
195 rdf:type schema:Organization
196 grid-institutes:grid.21729.3f schema:alternateName Department of Biological Sciences, Columbia University, New York, NY, USA
197 schema:name Department of Biological Sciences, Columbia University, New York, NY, USA
198 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...