DNA methylation increases throughout Arabidopsis development View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-10

AUTHORS

L. Ruiz-García, M. T. Cervera, J. M. Martínez-Zapater

ABSTRACT

We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. More... »

PAGES

301-306

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00425-005-1524-6

DOI

http://dx.doi.org/10.1007/s00425-005-1524-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013289128

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15968513


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