Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-09

AUTHORS

Matthew P. Purnell, Damianos S. Skopelitis, Kalliopi A. Roubelakis-Angelakis, José R. Botella

ABSTRACT

Glutamate dehydrogenase (GDH; EC 1.4.1.2-1.4.1.4) catalyses in vitro the reversible amination of 2-oxoglutarate to glutamate. In vascular plants the in vivo direction(s) of the GDH reaction and hence the physiological role(s) of this enzyme remain obscure. A phylogenetic analysis identified two clearly separated groups of higher-plant GDH genes encoding either the alpha- or beta-subunit of the GDH holoenzyme. To help clarify the physiological role(s) of GDH, tobacco (Nicotiana tabacum L.) was transformed with either an antisense or sense copy of a beta-subunit gene, and transgenic plants recovered with between 0.5- and 34-times normal leaf GDH activity. This large modulation of GDH activity (shown to be via alteration of beta-subunit levels) had little effect on leaf ammonium or the leaf free amino acid pool, except that a large increase in GDH activity was associated with a significant decrease in leaf Asp (~51%, P=0.0045). Similarly, plant growth and development were not affected, suggesting that a large modulation of GDH beta-subunit titre does not affect plant viability under the ideal growing conditions employed. Reduction of GDH activity and protein levels in an antisense line was associated with a large increase in transcripts of a beta-subunit gene, suggesting that the reduction in beta-subunit levels might have been due to translational inhibition. In another experiment designed to detect post-translational up-regulation of GDH activity, GDH over-expressing plants were subjected to prolonged dark-stress. GDH activity increased, but this was found to be due more likely to resistance of the GDH protein to stress-induced proteolysis, rather than to post-translational up-regulation. More... »

PAGES

167-180

References to SciGraph publications

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  • 2004-02. The enzymes and control of eukaryotic mRNA turnover in NATURE STRUCTURAL & MOLECULAR BIOLOGY
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  • 2000-06. Expression of the bacterial gdhA gene encoding a NADPH glutamate dehydrogenase in tobacco affects plant growth and development in PLANT AND SOIL
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  • Journal

    TITLE

    Planta

    ISSUE

    1

    VOLUME

    222

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00425-005-1510-z

    DOI

    http://dx.doi.org/10.1007/s00425-005-1510-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1037108391

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15803323


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