Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes View Full Text


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Article Info

DATE

2005-07-27

AUTHORS

Scott C. Schaefer, Ksenija Gasic, Bruno Cammue, Willem Broekaert, Els J. M. van Damme, Willy J. Peumans, Schuyler S. Korban

ABSTRACT

Genes coding for an iris ribosomal-inactivating protein (I-RIP), a maize β-glucanase (M-GLU), and a Mirabilis jalapa antimicrobial peptide (Mj-AMP1) were separately introduced into tomato (Lycopersicon esculentum cv. Sweet Chelsea) cotyledons via Agrobacterium tumefaciens-mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU, and Mj-AMP1 genes in various transgenic lines was determined using Northern blot analysis. Plants of selected transgenic lines were inoculated with a 2–3×104 conidial spores/ml suspension of the fungal pathogen Alternaria solani, the causal agent of tomato early blight. Compared to control (non-transformed) plants, two transgenic lines carrying either a M-GLU or Mj-AMP1 showed enhanced resistance to early blight disease. None of the four lines carrying the I-RIP transgene showed increased resistance to early blight. More... »

PAGES

858

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00425-005-0026-x

    DOI

    http://dx.doi.org/10.1007/s00425-005-0026-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003362687

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16047198


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