Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2004-12-17

AUTHORS

Hideyuki Suzuki, M. S. Srinivasa Reddy, Marina Naoumkina, Naveed Aziz, Gregory D. May, David V. Huhman, Lloyd W. Sumner, Jack W. Blount, Pedro Mendes, Richard A. Dixon

ABSTRACT

Exposure of cell suspension cultures of Medicago truncatula Gaerth. to methyl jasmonate (MeJA) resulted in up to 50-fold induction of transcripts encoding the key triterpene biosynthetic enzyme β-amyrin synthase (βAS; EC 5.4.99.-). Transcripts reached maximum levels at 24 h post-elicitation with 0.5 mM MeJA. The entry point enzymes into the phenylpropanoid and flavonoid pathways, l-phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and chalcone synthase (CHS; EC 2.3.1.74), respectively, were not induced by MeJA. In contrast, exposure of cells to yeast elicitor (YE) resulted in up to 45- and 14-fold induction of PAL and CHS transcripts, respectively, at only 2 h post-elicitation. βAS transcripts were weakly induced at 12 h after exposure to YE. Over 30 different triterpene saponins were identified in the cultures, many of which were strongly induced by MeJA, but not by YE. In contrast, cinnamic acids, benzoic acids and isoflavone-derived compounds accumulated following exposure of cultures to YE, but few changes in phenylpropanoid levels were observed in response to MeJA. DNA microarray analysis confirmed the strong differential transcriptional re-programming of the cell cultures for multiple genes in the phenylpropanoid and triterpene pathways in response to MeJA and YE, and indicated different responses of individual members of gene families. This work establishes Medicago cell cultures as an excellent model for future genomics approaches to understand the regulation of legume secondary metabolism. More... »

PAGES

696-707

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00425-004-1387-2

DOI

http://dx.doi.org/10.1007/s00425-004-1387-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003465778

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15605242


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33 schema:description Exposure of cell suspension cultures of Medicago truncatula Gaerth. to methyl jasmonate (MeJA) resulted in up to 50-fold induction of transcripts encoding the key triterpene biosynthetic enzyme β-amyrin synthase (βAS; EC 5.4.99.-). Transcripts reached maximum levels at 24 h post-elicitation with 0.5 mM MeJA. The entry point enzymes into the phenylpropanoid and flavonoid pathways, l-phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and chalcone synthase (CHS; EC 2.3.1.74), respectively, were not induced by MeJA. In contrast, exposure of cells to yeast elicitor (YE) resulted in up to 45- and 14-fold induction of PAL and CHS transcripts, respectively, at only 2 h post-elicitation. βAS transcripts were weakly induced at 12 h after exposure to YE. Over 30 different triterpene saponins were identified in the cultures, many of which were strongly induced by MeJA, but not by YE. In contrast, cinnamic acids, benzoic acids and isoflavone-derived compounds accumulated following exposure of cultures to YE, but few changes in phenylpropanoid levels were observed in response to MeJA. DNA microarray analysis confirmed the strong differential transcriptional re-programming of the cell cultures for multiple genes in the phenylpropanoid and triterpene pathways in response to MeJA and YE, and indicated different responses of individual members of gene families. This work establishes Medicago cell cultures as an excellent model for future genomics approaches to understand the regulation of legume secondary metabolism.
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41 DNA microarray analysis
42 Exposure of cultures
43 Gaerth
44 MeJA
45 Medicago cell cultures
46 Medicago truncatula
47 Medicago truncatula Gaerth
48 PAL
49 Ye
50 acid
51 amyrin synthase
52 analysis
53 biosynthetic enzyme β
54 cell cultures
55 cell suspension cultures
56 cells
57 chalcone synthase
58 changes
59 cinnamic acid
60 compounds
61 contrast
62 culture
63 different responses
64 different triterpene saponins
65 elicitors
66 entry point
67 enzyme β
68 excellent model
69 exposure
70 exposure of cells
71 family
72 flavonoid pathway
73 future genomics
74 gene family
75 genes
76 genomics
77 individual members
78 induction
79 induction of PAL
80 induction of transcripts
81 isoflavone-derived compounds
82 jasmonate
83 key triterpene biosynthetic enzyme β
84 legume Medicago truncatula
85 legume secondary metabolism
86 levels
87 mM MeJA
88 maximum level
89 members
90 metabolism
91 methyl jasmonate
92 microarray analysis
93 model
94 model legume Medicago truncatula
95 multiple genes
96 pathway
97 phenylalanine
98 phenylpropanoid levels
99 phenylpropanoids
100 point
101 regulation
102 response
103 saponins
104 secondary metabolism
105 suspension cultures
106 synthase
107 transcripts
108 triterpene biosynthetic enzyme β
109 triterpene pathway
110 triterpene saponins
111 truncatula
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113 work
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