Transforming petals into sepaloid organs in Arabidopsis and oilseed rape: implementation of the hairpin RNA-mediated gene silencing technology in an ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-01

AUTHORS

Marina Byzova, Christoph Verduyn, Dirk De Brouwer, Marc De Block

ABSTRACT

Oilseed rape ( Brassica napus L.) genotypes with no or small petals are thought to have advantages in photosynthetic activity. The flowers of field-grown oilseed rape form a bright-yellow canopy that reflects and absorbs nearly 60% of the photosynthetically active radiation (PAR), causing a severe yield penalty. Reducing the size of the petals and/or removing the reflecting colour will improve the transmission of PAR to the leaves and is expected to increase the crop productivity. In this study the 'hairpin' RNA-mediated (hpRNA) gene silencing technology was implemented in Arabidopsis thaliana (L.) Heynh. and B. napus to silence B-type MADS-box floral organ identity genes in a second-whorl-specific manner. In Arabidopsis, silencing of B-type MADS-box genes was obtained by expressing B. napus APETALA3( BAP3) or PISTILLATA ( BPI) homologous self-complementary hpRNA constructs under control of the Arabidopsis A-type MADS-box gene APETALA1 ( AP1) promoter. In B. napus, silencing of the BPI gene family was achieved by expressing a similar hpRNA construct as used in Arabidopsis under the control of a chimeric promoter consisting of a modified petal-specific Arabidopsis AP3 promoter fragment fused to the AP1 promoter. In this way, transgenic plants were generated producing male fertile flowers in which the petals were converted into sepals ( Arabidopsis) or into sepaloid petals ( B. napus). These novel flower phenotypes were stable and heritable in both species. More... »

PAGES

379-387

References to SciGraph publications

Journal

TITLE

Planta

ISSUE

3

VOLUME

218

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00425-003-1117-1

    DOI

    http://dx.doi.org/10.1007/s00425-003-1117-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1001560219

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/14534787


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    Turtle is a human-readable linked data format.

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    RDF/XML is a standard XML format for linked data.

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