Molecular evolution of C4 phosphoenolpyruvate carboxylase in the genus Flaveria—a gradual increase from C3 to C4 characteristics View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-09

AUTHORS

Sascha Engelmann, Oliver E. Bläsing, Udo Gowik, Per Svensson, Peter Westhoff

ABSTRACT

In order to elucidate the discrete steps in phospho enolpyruvate carboxylase (PEPC) evolution concerning K(m)-PEP and malate tolerance a comparison was made between C3, C3-C4 and C4 species of the dicot genus Flaveria. The PEPCs of this genus are encoded by a gene family comprising three classes: ppcA, ppcB and ppcC [J. Hermans and P. Westhoff (1990) Mol Gen Genet 224:459-468, (1992) Mol Gen Genet 234:275-284]. The ppcA of F trinervia (C4) codes for the C4 PEPC isoform but other plants of the genus contain ppcA orthologues too. The C3 plant F. pringlei showed the lowest levels of ppcA PEPC mRNA followed by F. pubescens (C3-C4) while the C4-like plant F. brownii displayed RNA amounts close to the C4 species F. trinervia. In contrast to the similar expression profiles of F. brownii (C4-like) and F. trinervia (C4) the PEPC amino acid sequence of F. brownii was more similar to the C3 and C3-C4 ppcA PEPCs than to the C4 PEPC. Similarly, the C3, C3-C4 and C4-like ppcA PEPCs showed almost identical PEP saturation kinetics when activated by glucose-6-phosphate ( K(m)-PEP: 17-20 microM) while the K(m)-PEP for the C4 PEPC was determined to be 53 microM. However, without activation the ppcA PEPCs of F. pubescens and F. brownii displayed C3-C4 intermediate values. A similar picture was obtained when the malate sensitivities were compared. In the non-activated state the F. trinervia (C4) enzyme was 10 times more tolerant to malate than the F. pringlei counterpart. The ppcA enzymes of F. pubescens (C3-C4) and F. brownii (C4-like) displayed intermediate values. In contrast, the inclusion of 5 mM glucose-6-phosphate in the reaction mixture changed the order totally. Interestingly, the activation rendered the C4 enzyme about 50% less tolerant to malate than the C3 PEPC. The activation had a positive effect on malate tolerance of the F. pubescens (C3-C4) PEPC while the ppcA PEPC of F. brownii (C4-like) was almost unaffected. More... »

PAGES

717-725

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00425-003-1045-0

DOI

http://dx.doi.org/10.1007/s00425-003-1045-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043934779

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12811556


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Evolution, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flaveria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Enzymologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucose-6-Phosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoenolpyruvate Carboxylase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Photosynthetic Reaction Center Complex Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phylogeny", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Heinrich Heine University D\u00fcsseldorf", 
          "id": "https://www.grid.ac/institutes/grid.411327.2", 
          "name": [
            "Institut f\u00fcr Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universit\u00e4t D\u00fcsseldorf, 40225, D\u00fcsseldorf, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Engelmann", 
        "givenName": "Sascha", 
        "id": "sg:person.01063732717.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063732717.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute for Molecular Plant Physiology", 
          "id": "https://www.grid.ac/institutes/grid.418390.7", 
          "name": [
            "Institut f\u00fcr Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universit\u00e4t D\u00fcsseldorf, 40225, D\u00fcsseldorf, Germany", 
            "Max-Planck-Institut f\u00fcr Molekulare Pflanzenphysiologie, Am M\u00fchlenberg 1, 14476, Golm, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bl\u00e4sing", 
        "givenName": "Oliver E.", 
        "id": "sg:person.0717015423.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717015423.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Heinrich Heine University D\u00fcsseldorf", 
          "id": "https://www.grid.ac/institutes/grid.411327.2", 
          "name": [
            "Institut f\u00fcr Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universit\u00e4t D\u00fcsseldorf, 40225, D\u00fcsseldorf, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gowik", 
        "givenName": "Udo", 
        "id": "sg:person.01016103411.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016103411.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swedish University of Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.6341.0", 
          "name": [
            "Department of Plant Biology, Swedish University of Agricultural Sciences, 750\u00a007, Uppsala, Sweden"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Svensson", 
        "givenName": "Per", 
        "id": "sg:person.0623775233.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623775233.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Heinrich Heine University D\u00fcsseldorf", 
          "id": "https://www.grid.ac/institutes/grid.411327.2", 
          "name": [
            "Institut f\u00fcr Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universit\u00e4t D\u00fcsseldorf, 40225, D\u00fcsseldorf, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Westhoff", 
        "givenName": "Peter", 
        "id": "sg:person.0715640752.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715640752.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/bioinformatics/12.4.357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000618136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(94)90168-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002362940"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/pcp/pcf019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003237182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.123.1.149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008273484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.51.3.439", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012256755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00041157", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012776681", 
          "https://doi.org/10.1007/bf00041157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00041157", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012776681", 
          "https://doi.org/10.1007/bf00041157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.10.7.1075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014879103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(90)90336-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016325531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(90)90336-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016325531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-3796(84)80041-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017885343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m909832199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020139786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.95.4.981", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021871475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(02)02975-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022185315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0968-0004(98)01285-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022203863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-012614440-6/50005-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022393572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.89.4.1129", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023173732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.010144", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024476836"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(84)90222-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026009644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(84)90222-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026009644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.82.3.695", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027269086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.67.2.330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028219656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00195340", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029020104", 
          "https://doi.org/10.1007/bf00195340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-3796(84)80039-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029089029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.arplant.47.1.273", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029137707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1997.t01-1-00452.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029917322"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.pcp.a029446", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030046216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0003-9861(03)00165-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032808975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0003-9861(03)00165-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032808975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00392131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035168749", 
          "https://doi.org/10.1007/bf00392131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00392131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035168749", 
          "https://doi.org/10.1007/bf00392131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3040.1989.tb01629.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038000001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(99)01103-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039210670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1006164209129", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040985657", 
          "https://doi.org/10.1023/a:1006164209129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.51.3.448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041133038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2610349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041330363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2610349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041330363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bi.46.070177.003041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042088378"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00283848", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045503142", 
          "https://doi.org/10.1007/bf00283848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00262441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046260289", 
          "https://doi.org/10.1007/bf00262441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00262441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046260289", 
          "https://doi.org/10.1007/bf00262441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(02)00913-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049761870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(02)00913-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049761870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.87.4.867", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051313472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-675410-0.50011-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051884658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80868-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053402026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/8.3.275", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059414153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3870712", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468573"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-09", 
    "datePublishedReg": "2003-09-01", 
    "description": "In order to elucidate the discrete steps in phospho enolpyruvate carboxylase (PEPC) evolution concerning K(m)-PEP and malate tolerance a comparison was made between C3, C3-C4 and C4 species of the dicot genus Flaveria. The PEPCs of this genus are encoded by a gene family comprising three classes: ppcA, ppcB and ppcC [J. Hermans and P. Westhoff (1990) Mol Gen Genet 224:459-468, (1992) Mol Gen Genet 234:275-284]. The ppcA of F trinervia (C4) codes for the C4 PEPC isoform but other plants of the genus contain ppcA orthologues too. The C3 plant F. pringlei showed the lowest levels of ppcA PEPC mRNA followed by F. pubescens (C3-C4) while the C4-like plant F. brownii displayed RNA amounts close to the C4 species F. trinervia. In contrast to the similar expression profiles of F. brownii (C4-like) and F. trinervia (C4) the PEPC amino acid sequence of F. brownii was more similar to the C3 and C3-C4 ppcA PEPCs than to the C4 PEPC. Similarly, the C3, C3-C4 and C4-like ppcA PEPCs showed almost identical PEP saturation kinetics when activated by glucose-6-phosphate ( K(m)-PEP: 17-20 microM) while the K(m)-PEP for the C4 PEPC was determined to be 53 microM. However, without activation the ppcA PEPCs of F. pubescens and F. brownii displayed C3-C4 intermediate values. A similar picture was obtained when the malate sensitivities were compared. In the non-activated state the F. trinervia (C4) enzyme was 10 times more tolerant to malate than the F. pringlei counterpart. The ppcA enzymes of F. pubescens (C3-C4) and F. brownii (C4-like) displayed intermediate values. In contrast, the inclusion of 5 mM glucose-6-phosphate in the reaction mixture changed the order totally. Interestingly, the activation rendered the C4 enzyme about 50% less tolerant to malate than the C3 PEPC. The activation had a positive effect on malate tolerance of the F. pubescens (C3-C4) PEPC while the ppcA PEPC of F. brownii (C4-like) was almost unaffected.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00425-003-1045-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "217"
      }
    ], 
    "name": "Molecular evolution of C4 phosphoenolpyruvate carboxylase in the genus Flaveria\u2014a gradual increase from C3 to C4 characteristics", 
    "pagination": "717-725", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d14740adc44c04bfb20edbc857a960e7e400fe7eb493158ae717f7a239b23d83"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12811556"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "1250576"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00425-003-1045-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1043934779"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00425-003-1045-0", 
      "https://app.dimensions.ai/details/publication/pub.1043934779"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00425-003-1045-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00425-003-1045-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00425-003-1045-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00425-003-1045-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00425-003-1045-0'


 

This table displays all metadata directly associated to this object as RDF triples.

282 TRIPLES      21 PREDICATES      82 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00425-003-1045-0 schema:about N030ce7722c3c436aae3b6758f75f3758
2 N28ef7401c89d413db7c74123df6fcd67
3 N2cb5aa2ae97e497590290acdca0e707d
4 N3476c4cc43a445c2a2c30030415693cf
5 N3763dee6ce584047a6431b3dbf60ebe2
6 N4ab8ac05e43d42b5883bdc57aa48ba9f
7 N615325648ae44318996a7990f8d5149b
8 N68e0fc18fd384398a666e21cae4d4586
9 N869ed1ef87494895b644e950a904c96e
10 Na5fde9a4a935490bb50828df421c34cb
11 Nb4949c9cee5e43c29fae18e7214cf850
12 Nc2a5534baa0d4289a47d23ed1881fd4a
13 Nc90c920530614400926348f0a13d9b13
14 anzsrc-for:06
15 anzsrc-for:0607
16 schema:author N0a0e50c315924a9da659d85d41236a2b
17 schema:citation sg:pub.10.1007/bf00041157
18 sg:pub.10.1007/bf00195340
19 sg:pub.10.1007/bf00262441
20 sg:pub.10.1007/bf00283848
21 sg:pub.10.1007/bf00392131
22 sg:pub.10.1023/a:1006164209129
23 https://doi.org/10.1016/0168-9452(94)90168-6
24 https://doi.org/10.1016/0378-1119(84)90222-1
25 https://doi.org/10.1016/0378-1119(90)90336-p
26 https://doi.org/10.1016/b978-0-12-675410-0.50011-7
27 https://doi.org/10.1016/b978-012614440-6/50005-7
28 https://doi.org/10.1016/s0003-9861(03)00165-6
29 https://doi.org/10.1016/s0014-5793(02)02975-7
30 https://doi.org/10.1016/s0014-5793(99)01103-5
31 https://doi.org/10.1016/s0015-3796(84)80039-6
32 https://doi.org/10.1016/s0015-3796(84)80041-4
33 https://doi.org/10.1016/s0092-8674(00)80868-5
34 https://doi.org/10.1016/s0968-0004(98)01285-7
35 https://doi.org/10.1016/s0969-2126(02)00913-9
36 https://doi.org/10.1042/bj2610349
37 https://doi.org/10.1074/jbc.m909832199
38 https://doi.org/10.1093/bioinformatics/12.4.357
39 https://doi.org/10.1093/bioinformatics/8.3.275
40 https://doi.org/10.1093/oxfordjournals.pcp.a029446
41 https://doi.org/10.1093/pcp/pcf019
42 https://doi.org/10.1104/pp.010144
43 https://doi.org/10.1104/pp.123.1.149
44 https://doi.org/10.1104/pp.51.3.439
45 https://doi.org/10.1104/pp.51.3.448
46 https://doi.org/10.1104/pp.67.2.330
47 https://doi.org/10.1104/pp.82.3.695
48 https://doi.org/10.1104/pp.87.4.867
49 https://doi.org/10.1104/pp.89.4.1129
50 https://doi.org/10.1104/pp.95.4.981
51 https://doi.org/10.1105/tpc.10.7.1075
52 https://doi.org/10.1111/j.1365-3040.1989.tb01629.x
53 https://doi.org/10.1111/j.1432-1033.1997.t01-1-00452.x
54 https://doi.org/10.1146/annurev.arplant.47.1.273
55 https://doi.org/10.1146/annurev.bi.46.070177.003041
56 https://doi.org/10.2307/3870712
57 schema:datePublished 2003-09
58 schema:datePublishedReg 2003-09-01
59 schema:description In order to elucidate the discrete steps in phospho enolpyruvate carboxylase (PEPC) evolution concerning K(m)-PEP and malate tolerance a comparison was made between C3, C3-C4 and C4 species of the dicot genus Flaveria. The PEPCs of this genus are encoded by a gene family comprising three classes: ppcA, ppcB and ppcC [J. Hermans and P. Westhoff (1990) Mol Gen Genet 224:459-468, (1992) Mol Gen Genet 234:275-284]. The ppcA of F trinervia (C4) codes for the C4 PEPC isoform but other plants of the genus contain ppcA orthologues too. The C3 plant F. pringlei showed the lowest levels of ppcA PEPC mRNA followed by F. pubescens (C3-C4) while the C4-like plant F. brownii displayed RNA amounts close to the C4 species F. trinervia. In contrast to the similar expression profiles of F. brownii (C4-like) and F. trinervia (C4) the PEPC amino acid sequence of F. brownii was more similar to the C3 and C3-C4 ppcA PEPCs than to the C4 PEPC. Similarly, the C3, C3-C4 and C4-like ppcA PEPCs showed almost identical PEP saturation kinetics when activated by glucose-6-phosphate ( K(m)-PEP: 17-20 microM) while the K(m)-PEP for the C4 PEPC was determined to be 53 microM. However, without activation the ppcA PEPCs of F. pubescens and F. brownii displayed C3-C4 intermediate values. A similar picture was obtained when the malate sensitivities were compared. In the non-activated state the F. trinervia (C4) enzyme was 10 times more tolerant to malate than the F. pringlei counterpart. The ppcA enzymes of F. pubescens (C3-C4) and F. brownii (C4-like) displayed intermediate values. In contrast, the inclusion of 5 mM glucose-6-phosphate in the reaction mixture changed the order totally. Interestingly, the activation rendered the C4 enzyme about 50% less tolerant to malate than the C3 PEPC. The activation had a positive effect on malate tolerance of the F. pubescens (C3-C4) PEPC while the ppcA PEPC of F. brownii (C4-like) was almost unaffected.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree false
63 schema:isPartOf Nc312470b69824d50b7419fa2a9b6d944
64 Nf143951af8c84892943e091543a66595
65 sg:journal.1054035
66 schema:name Molecular evolution of C4 phosphoenolpyruvate carboxylase in the genus Flaveria—a gradual increase from C3 to C4 characteristics
67 schema:pagination 717-725
68 schema:productId N04cb47c0fffe4b188529bcd0de261101
69 N368a9a67bc714c4db17d0e33ebf4e870
70 N4b51b51ae93c4d59bd7a58bc06448002
71 N76040166949c43a2bdbb868882f875f8
72 Ne8047a145d4047478686e66490d173af
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043934779
74 https://doi.org/10.1007/s00425-003-1045-0
75 schema:sdDatePublished 2019-04-10T17:32
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher N12f0c433ac4248629235420618d8cfce
78 schema:url http://link.springer.com/10.1007%2Fs00425-003-1045-0
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N0189f2925aa242b28eac11ea90ae03f8 rdf:first sg:person.0717015423.45
83 rdf:rest N9227a08989574f4aa2b3645fe79856fb
84 N030ce7722c3c436aae3b6758f75f3758 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Gene Expression Regulation, Plant
86 rdf:type schema:DefinedTerm
87 N04cb47c0fffe4b188529bcd0de261101 schema:name dimensions_id
88 schema:value pub.1043934779
89 rdf:type schema:PropertyValue
90 N0a0e50c315924a9da659d85d41236a2b rdf:first sg:person.01063732717.09
91 rdf:rest N0189f2925aa242b28eac11ea90ae03f8
92 N12f0c433ac4248629235420618d8cfce schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 N28ef7401c89d413db7c74123df6fcd67 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Kinetics
96 rdf:type schema:DefinedTerm
97 N2a04f0c8f9fb4c839d74719d4d02ea93 rdf:first sg:person.0623775233.29
98 rdf:rest N832c1dcf5478413ab524370a08f88617
99 N2cb5aa2ae97e497590290acdca0e707d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Gene Expression Regulation, Enzymologic
101 rdf:type schema:DefinedTerm
102 N3476c4cc43a445c2a2c30030415693cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Flaveria
104 rdf:type schema:DefinedTerm
105 N368a9a67bc714c4db17d0e33ebf4e870 schema:name nlm_unique_id
106 schema:value 1250576
107 rdf:type schema:PropertyValue
108 N3763dee6ce584047a6431b3dbf60ebe2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Phylogeny
110 rdf:type schema:DefinedTerm
111 N4ab8ac05e43d42b5883bdc57aa48ba9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Amino Acid Sequence
113 rdf:type schema:DefinedTerm
114 N4b51b51ae93c4d59bd7a58bc06448002 schema:name readcube_id
115 schema:value d14740adc44c04bfb20edbc857a960e7e400fe7eb493158ae717f7a239b23d83
116 rdf:type schema:PropertyValue
117 N615325648ae44318996a7990f8d5149b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Photosynthetic Reaction Center Complex Proteins
119 rdf:type schema:DefinedTerm
120 N68e0fc18fd384398a666e21cae4d4586 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Phosphoenolpyruvate Carboxylase
122 rdf:type schema:DefinedTerm
123 N76040166949c43a2bdbb868882f875f8 schema:name doi
124 schema:value 10.1007/s00425-003-1045-0
125 rdf:type schema:PropertyValue
126 N832c1dcf5478413ab524370a08f88617 rdf:first sg:person.0715640752.80
127 rdf:rest rdf:nil
128 N869ed1ef87494895b644e950a904c96e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Glucose-6-Phosphate
130 rdf:type schema:DefinedTerm
131 N9227a08989574f4aa2b3645fe79856fb rdf:first sg:person.01016103411.92
132 rdf:rest N2a04f0c8f9fb4c839d74719d4d02ea93
133 Na5fde9a4a935490bb50828df421c34cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Sequence Homology, Amino Acid
135 rdf:type schema:DefinedTerm
136 Nb4949c9cee5e43c29fae18e7214cf850 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Molecular Sequence Data
138 rdf:type schema:DefinedTerm
139 Nc2a5534baa0d4289a47d23ed1881fd4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Evolution, Molecular
141 rdf:type schema:DefinedTerm
142 Nc312470b69824d50b7419fa2a9b6d944 schema:issueNumber 5
143 rdf:type schema:PublicationIssue
144 Nc90c920530614400926348f0a13d9b13 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Malates
146 rdf:type schema:DefinedTerm
147 Ne8047a145d4047478686e66490d173af schema:name pubmed_id
148 schema:value 12811556
149 rdf:type schema:PropertyValue
150 Nf143951af8c84892943e091543a66595 schema:volumeNumber 217
151 rdf:type schema:PublicationVolume
152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biological Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
156 schema:name Plant Biology
157 rdf:type schema:DefinedTerm
158 sg:journal.1054035 schema:issn 0032-0935
159 1432-2048
160 schema:name Planta
161 rdf:type schema:Periodical
162 sg:person.01016103411.92 schema:affiliation https://www.grid.ac/institutes/grid.411327.2
163 schema:familyName Gowik
164 schema:givenName Udo
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016103411.92
166 rdf:type schema:Person
167 sg:person.01063732717.09 schema:affiliation https://www.grid.ac/institutes/grid.411327.2
168 schema:familyName Engelmann
169 schema:givenName Sascha
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063732717.09
171 rdf:type schema:Person
172 sg:person.0623775233.29 schema:affiliation https://www.grid.ac/institutes/grid.6341.0
173 schema:familyName Svensson
174 schema:givenName Per
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623775233.29
176 rdf:type schema:Person
177 sg:person.0715640752.80 schema:affiliation https://www.grid.ac/institutes/grid.411327.2
178 schema:familyName Westhoff
179 schema:givenName Peter
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715640752.80
181 rdf:type schema:Person
182 sg:person.0717015423.45 schema:affiliation https://www.grid.ac/institutes/grid.418390.7
183 schema:familyName Bläsing
184 schema:givenName Oliver E.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717015423.45
186 rdf:type schema:Person
187 sg:pub.10.1007/bf00041157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012776681
188 https://doi.org/10.1007/bf00041157
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/bf00195340 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029020104
191 https://doi.org/10.1007/bf00195340
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/bf00262441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046260289
194 https://doi.org/10.1007/bf00262441
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/bf00283848 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045503142
197 https://doi.org/10.1007/bf00283848
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/bf00392131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035168749
200 https://doi.org/10.1007/bf00392131
201 rdf:type schema:CreativeWork
202 sg:pub.10.1023/a:1006164209129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040985657
203 https://doi.org/10.1023/a:1006164209129
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/0168-9452(94)90168-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002362940
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/0378-1119(84)90222-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026009644
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/0378-1119(90)90336-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1016325531
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/b978-0-12-675410-0.50011-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051884658
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/b978-012614440-6/50005-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022393572
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0003-9861(03)00165-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032808975
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s0014-5793(02)02975-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022185315
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/s0014-5793(99)01103-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039210670
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/s0015-3796(84)80039-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029089029
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/s0015-3796(84)80041-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017885343
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/s0092-8674(00)80868-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053402026
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/s0968-0004(98)01285-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022203863
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/s0969-2126(02)00913-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049761870
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1042/bj2610349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041330363
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1074/jbc.m909832199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020139786
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1093/bioinformatics/12.4.357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000618136
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/bioinformatics/8.3.275 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059414153
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/oxfordjournals.pcp.a029446 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030046216
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/pcp/pcf019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003237182
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1104/pp.010144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024476836
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1104/pp.123.1.149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008273484
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1104/pp.51.3.439 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012256755
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1104/pp.51.3.448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041133038
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1104/pp.67.2.330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028219656
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1104/pp.82.3.695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027269086
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1104/pp.87.4.867 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051313472
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1104/pp.89.4.1129 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023173732
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1104/pp.95.4.981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021871475
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1105/tpc.10.7.1075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014879103
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1111/j.1365-3040.1989.tb01629.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038000001
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1111/j.1432-1033.1997.t01-1-00452.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029917322
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1146/annurev.arplant.47.1.273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029137707
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1146/annurev.bi.46.070177.003041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042088378
270 rdf:type schema:CreativeWork
271 https://doi.org/10.2307/3870712 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468573
272 rdf:type schema:CreativeWork
273 https://www.grid.ac/institutes/grid.411327.2 schema:alternateName Heinrich Heine University Düsseldorf
274 schema:name Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany
275 rdf:type schema:Organization
276 https://www.grid.ac/institutes/grid.418390.7 schema:alternateName Max Planck Institute for Molecular Plant Physiology
277 schema:name Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany
278 Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476, Golm, Germany
279 rdf:type schema:Organization
280 https://www.grid.ac/institutes/grid.6341.0 schema:alternateName Swedish University of Agricultural Sciences
281 schema:name Department of Plant Biology, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden
282 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...