β-Ketoacyl-acyl carrier protein synthase IV: a key enzyme for regulation of medium-chain fatty acid synthesis in Cuphea lanceolata seeds View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-09

AUTHORS

Burkhardt Schütt, Amine Abbadi, Brigitte Loddenkötter, Monika Brummel, Friedrich Spener

ABSTRACT

With the aim of elucidating the mechanisms involved in the biosynthesis of medium-chain fatty acids in Cuphea lanceolata Ait., a crop accumulating up to 90% decanoic acid in seed triacylglycerols, cDNA clones of a beta-ketoacyl-acyl carrier protein (ACP) synthase IV (clKAS IV, EC 2.3.1.41) were isolated from C. lanceolata seed embryos. The amino acid sequence deduced from clKAS IV cDNA showed 80% identity to other plant KAS II-type enzymes, 55% identity towards plant KAS I and over 90% towards other Cuphea KAS IV-type sequences. Recombinant clKAS IV was functionally overexpressed in Escherichia coli, and substrate specificity of purified enzyme showed strong preference for elongation of short-chain and medium-chain acyl-ACPs (C4- to C10-ACP) with nearly equal activity. Further elongation steps were catalysed with distinctly less activity. Moreover, short- and medium-chain acyl-ACPs exerted a chain-length-specific and concentration-dependent substrate inhibition of clKAS IV. Based on these findings a regulatory mechanism for medium-chain fatty acid synthesis in C. lanceolata is presented. More... »

PAGES

847-854

Journal

TITLE

Planta

ISSUE

5

VOLUME

215

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00425-002-0803-8

DOI

http://dx.doi.org/10.1007/s00425-002-0803-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026559147

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12244451


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