hPSC gene editing for cardiac disease therapy View Full Text


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Article Info

DATE

2022-09-27

AUTHORS

Amina Saleem, Muhammad Khawar Abbas, Yongming Wang, Feng Lan

ABSTRACT

Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide. However, the lack of human cardiomyocytes with proper genetic backgrounds limits the study of disease mechanisms. Human pluripotent stem cell–derived cardiomyocytes (hPSC-CMs) have significantly advanced the study of these conditions. Moreover, hPSC-CMs made it easy to study CVDs using genome-editing techniques. This article discusses the applications of these techniques in hPSC for studying CVDs. Recently, several genome-editing systems have been used to modify hPSCs, including zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeat-associated protein 9 (CRISPR/Cas9). We focused on the recent advancement of genome editing in hPSCs, which dramatically improved the efficiency of the cell-based mechanism study and therapy for cardiac diseases.Graphical abstract More... »

PAGES

1123-1132

References to SciGraph publications

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  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/s00424-022-02751-2

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