Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural ... View Full Text


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Article Info

DATE

2022-08-06

AUTHORS

I. Y. Melnikov, Sergey A. Tyganov, K. A. Sharlo, A. D. Ulanova, I. M. Vikhlyantsev, T. M. Mirzoev, B. S. Shenkman

ABSTRACT

In mammals, prolonged mechanical unloading results in a significant decrease in passive stiffness of postural muscles. The nature of this phenomenon remains unclear. The aim of the present study was to investigate possible causes for a reduction in rat soleus passive stiffness after 7 and 14 days of unloading (hindlimb suspension, HS). We hypothesized that HS-induced decrease in passive stiffness would be associated with calpain-dependent degradation of cytoskeletal proteins or a decrease in actomyosin interaction. Wistar rats were subjected to HS for 7 and 14 days with or without PD150606 (calpain inhibitor) treatment. Soleus muscles were subjected to biochemical analysis and ex vivo measurements of passive tension with or without blebbistatin treatment (an inhibitor of actomyosin interactions). Passive tension of isolated soleus muscle was significantly reduced after 7- and 14-day HS compared to the control values. PD150606 treatment during 7- and 14-day HS induced an increase in alpha-actinin-2 and -3, desmin contents compared to control, partly prevented a decrease in intact titin (T1) content, and prevented a decrease in soleus passive tension. Incubation of soleus muscle with blebbistatin did not affect HS-induced reductions in specific passive tension in soleus muscle. Our study suggests that calpain-dependent breakdown of cytoskeletal proteins, but not a change in actomyosin interaction, significantly contributes to unloading-induced reductions in intrinsic passive stiffness of rat soleus muscle. More... »

PAGES

1171-1183

References to SciGraph publications

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  • 1995-01. Changes in stiffness induced by hindlimb suspension in rat soleus muscle in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2010-08-17. The basal calcium level in fibers of the rat soleus muscle under gravitational unloading: The mechanisms of its increase and the role in calpain activation in DOKLADY BIOLOGICAL SCIENCES
  • 2021-05-07. Plantar mechanical stimulation attenuates protein synthesis decline in disused skeletal muscle via modulation of nitric oxide level in SCIENTIFIC REPORTS
  • 2007-04-12. Calpain/calpastatin activities and substrate depletion patterns during hindlimb unweighting and reweighting in skeletal muscle in EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
  • 2012-05-28. Leaky ryanodine receptors in β-sarcoglycan deficient mice: a potential common defect in muscular dystrophy in SKELETAL MUSCLE
  • 2018-07. Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains? in DOKLADY BIOCHEMISTRY AND BIOPHYSICS
  • 1988. Gravitational Mechanisms in the Motor System. Studies in Real and Simulated Weightlessness in STANCE AND MOTION
  • 2007-11-10. Blebbistatin: use as inhibitor of muscle contraction in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2008-02. The role of L-type calcium channels in the accumulation of Ca2+ in soleus muscle fibers in the rat and changes in the ratio of myosin and serca isoforms in conditions of gravitational unloading in NEUROSCIENCE AND BEHAVIORAL PHYSIOLOGY
  • 2012-05. Desmin and α-actinin-2 content in rat soleus muscle in the dynamics of gravitational unloading and subsequent reloading in DOKLADY BIOCHEMISTRY AND BIOPHYSICS
  • 2011-05-21. Protective effect of L-arginine administration on proteins of unloaded m. soleus in BIOCHEMISTRY (MOSCOW)
  • 2005-03-06. The structural basis of blebbistatin inhibition and specificity for myosin II in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2017-05-29. Nuances of electrophoresis study of titin/connectin in BIOPHYSICAL REVIEWS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00424-022-02740-5

    DOI

    http://dx.doi.org/10.1007/s00424-022-02740-5

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35931829


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    32 schema:description Abstract In mammals, prolonged mechanical unloading results in a significant decrease in passive stiffness of postural muscles. The nature of this phenomenon remains unclear. The aim of the present study was to investigate possible causes for a reduction in rat soleus passive stiffness after 7 and 14 days of unloading (hindlimb suspension, HS). We hypothesized that HS-induced decrease in passive stiffness would be associated with calpain-dependent degradation of cytoskeletal proteins or a decrease in actomyosin interaction. Wistar rats were subjected to HS for 7 and 14 days with or without PD150606 (calpain inhibitor) treatment. Soleus muscles were subjected to biochemical analysis and ex vivo measurements of passive tension with or without blebbistatin treatment (an inhibitor of actomyosin interactions). Passive tension of isolated soleus muscle was significantly reduced after 7- and 14-day HS compared to the control values. PD150606 treatment during 7- and 14-day HS induced an increase in alpha-actinin-2 and -3, desmin contents compared to control, partly prevented a decrease in intact titin (T1) content, and prevented a decrease in soleus passive tension. Incubation of soleus muscle with blebbistatin did not affect HS-induced reductions in specific passive tension in soleus muscle. Our study suggests that calpain-dependent breakdown of cytoskeletal proteins, but not a change in actomyosin interaction, significantly contributes to unloading-induced reductions in intrinsic passive stiffness of rat soleus muscle.
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