Ontology type: schema:ScholarlyArticle
2005-06-11
AUTHORSGergely Szabó, Norbert Szentandrássy, Tamás Bíró, Balázs I. Tóth, Gabriella Czifra, János Magyar, Tamás Bányász, András Varró, László Kovács, Péter P. Nánási
ABSTRACTThe aim of the present study was to compare the distribution of ion currents and the major underlying ion channel proteins in canine and human subepicardial (EPI) and midmyocardial (MID) left-ventricular muscle. Ion currents and action potentials were recorded from canine cardiomyocytes derived from the very superficial EPI and central MID regions of the left ventricle. Amplitude, duration and the maximum velocity of depolarization of the action potential were significantly greater in MID than EPI myocytes, whereas phase-1 repolarization was more pronounced in the EPI cells. Amplitudes of the transient outwards K+ current (29.5±1.5 vs. 19.0±2.3 pA/pF at +50 mV) and the slow component of the delayed rectifier K+ current (10.3±2.3 vs. 6.5±1.0 pA/pF at +50 mV) were significantly larger in EPI than in MID myocytes under whole-cell voltage-clamp conditions. The densities of the inwards rectifier K+ current, rapid delayed rectifier K+ current and L-type Ca2+ current were similar in both cell types. Expression of channel proteins in both canine and human ventricular myocardium was determined by Western blotting. In the canine heart, the expression of Kv4.3, Kv1.4, KChIP2 and KvLQT1 was significantly higher, and that of Nav1.5 and MinK much lower, in EPI than in MID. No significant EPI-MID differences were observed in the expression of the other channel proteins studied (Kir2.1, α1C, HERG and MiRP1). Similar results were obtained in human hearts, although the HERG was more abundant in the EPI than in the MID layer. In the canine heart, the EPI-MID differences in ion current densities were proportional to differences in channel protein expression. Except for the density of HERG, the pattern of EPI-MID distribution of ion-channel proteins was identical in canine and human ventricles. More... »
PAGES307-316
http://scigraph.springernature.com/pub.10.1007/s00424-005-1445-z
DOIhttp://dx.doi.org/10.1007/s00424-005-1445-z
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1028069154
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/15952036
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Action Potentials",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Blotting, Western",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Calcium Channels, L-Type",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Dogs",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ether-A-Go-Go Potassium Channels",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ion Channels",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Male",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Membrane Potentials",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Myocytes, Cardiac",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pericardium",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ventricular Function",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "Szab\u00f3",
"givenName": "Gergely",
"id": "sg:person.01247642013.32",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247642013.32"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "Szentandr\u00e1ssy",
"givenName": "Norbert",
"id": "sg:person.0741603700.70",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741603700.70"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "B\u00edr\u00f3",
"givenName": "Tam\u00e1s",
"id": "sg:person.01202774703.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202774703.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "T\u00f3th",
"givenName": "Bal\u00e1zs I.",
"id": "sg:person.01242130467.37",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242130467.37"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "Czifra",
"givenName": "Gabriella",
"id": "sg:person.0645470005.78",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645470005.78"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "Magyar",
"givenName": "J\u00e1nos",
"id": "sg:person.01206027354.83",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206027354.83"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "B\u00e1ny\u00e1sz",
"givenName": "Tam\u00e1s",
"id": "sg:person.0772313221.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772313221.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pharmacology and Pharmacotherapy, University of Szeged, P.O.Box 427, 6701, Szeged, Hungary",
"id": "http://www.grid.ac/institutes/grid.9008.1",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"Department of Pharmacology and Pharmacotherapy, University of Szeged, P.O.Box 427, 6701, Szeged, Hungary"
],
"type": "Organization"
},
"familyName": "Varr\u00f3",
"givenName": "Andr\u00e1s",
"id": "sg:person.015203014040.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015203014040.44"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "Kov\u00e1cs",
"givenName": "L\u00e1szl\u00f3",
"id": "sg:person.01241275126.40",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241275126.40"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary",
"id": "http://www.grid.ac/institutes/grid.7122.6",
"name": [
"Department of Physiology, University of Debrecen, P.O.Box 22, 4012, Debrecen, Hungary"
],
"type": "Organization"
},
"familyName": "N\u00e1n\u00e1si",
"givenName": "P\u00e9ter P.",
"id": "sg:person.0724365203.35",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724365203.35"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s002100000379",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046688341",
"https://doi.org/10.1007/s002100000379"
],
"type": "CreativeWork"
}
],
"datePublished": "2005-06-11",
"datePublishedReg": "2005-06-11",
"description": "The aim of the present study was to compare the distribution of ion currents and the major underlying ion channel proteins in canine and human subepicardial (EPI) and midmyocardial (MID) left-ventricular muscle. Ion currents and action potentials were recorded from canine cardiomyocytes derived from the very superficial EPI and central MID regions of the left ventricle. Amplitude, duration and the maximum velocity of depolarization of the action potential were significantly greater in MID than EPI myocytes, whereas phase-1 repolarization was more pronounced in the EPI cells. Amplitudes of the transient outwards K+ current (29.5\u00b11.5 vs. 19.0\u00b12.3\u00a0pA/pF at +50\u00a0mV) and the slow component of the delayed rectifier K+ current (10.3\u00b12.3 vs. 6.5\u00b11.0\u00a0pA/pF at +50\u00a0mV) were significantly larger in EPI than in MID myocytes under whole-cell voltage-clamp conditions. The densities of the inwards rectifier K+ current, rapid delayed rectifier K+ current and L-type Ca2+ current were similar in both cell types. Expression of channel proteins in both canine and human ventricular myocardium was determined by Western blotting. In the canine heart, the expression of Kv4.3, Kv1.4, KChIP2 and KvLQT1 was significantly higher, and that of Nav1.5 and MinK much lower, in EPI than in MID. No significant EPI-MID differences were observed in the expression of the other channel proteins studied (Kir2.1, \u03b11C, HERG and MiRP1). Similar results were obtained in human hearts, although the HERG was more abundant in the EPI than in the MID layer. In the canine heart, the EPI-MID differences in ion current densities were proportional to differences in channel protein expression. Except for the density of HERG, the pattern of EPI-MID distribution of ion-channel proteins was identical in canine and human ventricles.",
"genre": "article",
"id": "sg:pub.10.1007/s00424-005-1445-z",
"inLanguage": "en",
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.4189212",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.4190636",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.4190632",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1005222",
"issn": [
"0031-6768",
"1432-2013"
],
"name": "Pfl\u00fcgers Archiv - European Journal of Physiology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "5",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "450"
}
],
"keywords": [
"action potentials",
"canine heart",
"expression of Kv4.3",
"whole-cell voltage-clamp conditions",
"channel protein expression",
"left ventricular muscle",
"human ventricular myocardium",
"left ventricular wall",
"voltage-clamp conditions",
"type Ca2",
"transient outward",
"ventricular myocardium",
"phase 1 repolarization",
"left ventricle",
"canine cardiomyocytes",
"human ventricle",
"ion channel proteins",
"channel proteins",
"Epi myocytes",
"Western blotting",
"protein expression",
"inward rectifier",
"human heart",
"canine",
"ventricle",
"heart",
"ion channels",
"myocytes",
"cell types",
"hERG",
"present study",
"expression",
"slow component",
"mid region",
"similar results",
"EPI",
"EPI cells",
"protein",
"myocardium",
"differences",
"repolarization",
"Kv4.3",
"subepicardial",
"muscle",
"cardiomyocytes",
"blotting",
"KChIP2",
"Nav1.5",
"Kv1.4",
"midmyocardium",
"depolarization",
"epicardium",
"KvLQT1",
"duration",
"ion current",
"Ca2",
"cells",
"aim",
"mink",
"maximum velocity",
"study",
"potential",
"mid",
"amplitude",
"asymmetrical distribution",
"patterns",
"mid layer",
"wall",
"types",
"results",
"outwards",
"distribution",
"region",
"components",
"conditions",
"density",
"current",
"channels",
"velocity",
"rectifier",
"layer",
"current density",
"ion current density"
],
"name": "Asymmetrical distribution of ion channels in canine and human left-ventricular wall: epicardium versus midmyocardium",
"pagination": "307-316",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1028069154"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00424-005-1445-z"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"15952036"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00424-005-1445-z",
"https://app.dimensions.ai/details/publication/pub.1028069154"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:23",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_404.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00424-005-1445-z"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00424-005-1445-z'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00424-005-1445-z'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00424-005-1445-z'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00424-005-1445-z'
This table displays all metadata directly associated to this object as RDF triples.
278 TRIPLES
22 PREDICATES
124 URIs
115 LITERALS
21 BLANK NODES