The role of cell type in bone healing mediated by ex vivo gene therapy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-10

AUTHORS

Tim Rose, Hairong Peng, Hsain-Chung Shen, Arvydas Usas, Ryosuke Kuroda, Helmut Lill, Freddie H. Fu, Johnny Huard

ABSTRACT

BACKGROUND: The ideal cellular vehicle for use in cell-mediated gene therapy to enhance bone healing has not yet been identified. The purpose of this study was to compare the capacity of two types of cells transduced with retro-bone morphogenetic protein 4 (BMP4)-muscle-derived cells (MDCs) and unfractioned bone marrow stromal cells (BMSCs). METHOD: Primary rat MDCs and unfractioned rat BMSCs were transduced with a retrovirus to express BMP4. A 7-mm, critical-sized femur defect was created in adult rats, and 5 x 10(6) transduced cells were implanted into the femoral defect. Bone healing was monitored radiographically and histologically at 4, 8, and 12 weeks post-implantation. RESULTS: All specimens in the MDC-BMP4 group and BMSC-BMP4 group showed a bridging callus at 8 and 12 weeks. At 12 weeks post-implantation the calluses of the MDC-BMP4 femora displayed significantly higher bone photodensity than the BMSC-BMP4 femora (P<0.05). Histomorphometry revealed no difference between the two treatment groups. However, non-union between newly formed and original bone was observed in none of the MDC femora but in six femora from the BMSC-BMP4 group. CONCLUSION: Both MDCs and unfractioned BMSCs can improve healing of a critical-sized bone defect following transduction of the cells with retroBMP4. However, MDCs appear to yield superior results when compared with BMSCs in terms of improved healing of segmental defects. More... »

PAGES

347-355

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00423-003-0401-7

DOI

http://dx.doi.org/10.1007/s00423-003-0401-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038324499

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14534793


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Marrow Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Morphogenetic Protein 4", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Morphogenetic Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chondrocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Inbred F344", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stromal Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Wound Healing", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Department of Trauma and Reconstructive Surgery, University of Leipzig, Liebigstrasse 20a, 04103 Leipzig, Germany", 
            "Growth and Development Laboratory, Department of Orthopedic Surgery, Children\u2019s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rose", 
        "givenName": "Tim", 
        "id": "sg:person.01236371200.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236371200.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Growth and Development Laboratory, Department of Orthopedic Surgery, Children\u2019s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peng", 
        "givenName": "Hairong", 
        "id": "sg:person.01317744350.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317744350.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tri-Service General Hospital", 
          "id": "https://www.grid.ac/institutes/grid.278244.f", 
          "name": [
            "Growth and Development Laboratory, Department of Orthopedic Surgery, Children\u2019s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA", 
            "Department of Orthopedic Surgery, Tri-Service General Hospital, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shen", 
        "givenName": "Hsain-Chung", 
        "id": "sg:person.0742403260.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742403260.89"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Growth and Development Laboratory, Department of Orthopedic Surgery, Children\u2019s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Usas", 
        "givenName": "Arvydas", 
        "id": "sg:person.0777360524.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777360524.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Growth and Development Laboratory, Department of Orthopedic Surgery, Children\u2019s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA", 
            "Department of Orthopedics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kuroda", 
        "givenName": "Ryosuke", 
        "id": "sg:person.01025022257.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025022257.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Leipzig University", 
          "id": "https://www.grid.ac/institutes/grid.9647.c", 
          "name": [
            "Department of Trauma and Reconstructive Surgery, University of Leipzig, Liebigstrasse 20a, 04103 Leipzig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lill", 
        "givenName": "Helmut", 
        "id": "sg:person.01034641540.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034641540.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Department of Orthopedics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fu", 
        "givenName": "Freddie H.", 
        "id": "sg:person.0706577466.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706577466.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Growth and Development Laboratory, Department of Orthopedic Surgery, Children\u2019s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huard", 
        "givenName": "Johnny", 
        "id": "sg:person.0603751545.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603751545.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/1357-4310(96)88792-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001332045"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007632-199609150-00012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003885165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007632-199609150-00012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003885165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007632-199609150-00012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003885165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001130050443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004578554", 
          "https://doi.org/10.1007/s001130050443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.142.5.1257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007731134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jor.1100180613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012800545"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1357-4310(98)01231-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022007968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm199512073332308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024758674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jor.1100140115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026460241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mthe.2002.0654", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028433647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301772", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031454600", 
          "https://doi.org/10.1038/sj.gt.3301772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301772", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031454600", 
          "https://doi.org/10.1038/sj.gt.3301772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci15153", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035029632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s8756-3282(97)00074-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041183246"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/10430340050015248", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059201901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/104303402320138989", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059202336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-200009000-00040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060168389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-200009000-00040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060168389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-200009000-00040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060168389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-200210001-00028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060169430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-200210001-00028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060169430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-200210001-00028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060169430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.150.3698.893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062487133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2106/00004623-198062070-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068887424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2106/00004623-199907000-00002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068893402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2106/00004623-200107000-00008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068894485"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082647163", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082950642", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-10", 
    "datePublishedReg": "2003-10-01", 
    "description": "BACKGROUND: The ideal cellular vehicle for use in cell-mediated gene therapy to enhance bone healing has not yet been identified. The purpose of this study was to compare the capacity of two types of cells transduced with retro-bone morphogenetic protein 4 (BMP4)-muscle-derived cells (MDCs) and unfractioned bone marrow stromal cells (BMSCs).\nMETHOD: Primary rat MDCs and unfractioned rat BMSCs were transduced with a retrovirus to express BMP4. A 7-mm, critical-sized femur defect was created in adult rats, and 5 x 10(6) transduced cells were implanted into the femoral defect. Bone healing was monitored radiographically and histologically at 4, 8, and 12 weeks post-implantation.\nRESULTS: All specimens in the MDC-BMP4 group and BMSC-BMP4 group showed a bridging callus at 8 and 12 weeks. At 12 weeks post-implantation the calluses of the MDC-BMP4 femora displayed significantly higher bone photodensity than the BMSC-BMP4 femora (P<0.05). Histomorphometry revealed no difference between the two treatment groups. However, non-union between newly formed and original bone was observed in none of the MDC femora but in six femora from the BMSC-BMP4 group.\nCONCLUSION: Both MDCs and unfractioned BMSCs can improve healing of a critical-sized bone defect following transduction of the cells with retroBMP4. However, MDCs appear to yield superior results when compared with BMSCs in terms of improved healing of segmental defects.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00423-003-0401-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1295031", 
        "issn": [
          "1435-2443", 
          "1435-2451"
        ], 
        "name": "Langenbeck's Archives of Surgery", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "388"
      }
    ], 
    "name": "The role of cell type in bone healing mediated by ex vivo gene therapy", 
    "pagination": "347-355", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b69042a0921e1222da37e475721c1991dd321178806c5e5934270fd180fd0edc"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "14534793"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9808285"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00423-003-0401-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038324499"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00423-003-0401-7", 
      "https://app.dimensions.ai/details/publication/pub.1038324499"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000490.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s00423-003-0401-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00423-003-0401-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00423-003-0401-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00423-003-0401-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00423-003-0401-7'


 

This table displays all metadata directly associated to this object as RDF triples.

241 TRIPLES      21 PREDICATES      63 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00423-003-0401-7 schema:about N3d020c327aaa416fa129ed042dc230a6
2 N451c5f44ae0447389fceb85447ec2836
3 N543db2846373439288d292fb6f4e0a7a
4 N8298c72354f243e6abfc602f89c03b40
5 N82a4cb133be645bf9f832a5dd8c63940
6 N8b2a660c81f44b7d9539b3c6302de6c2
7 N9dd1d4e60e3748cfb9a58e8b5413a011
8 Na7d5dd94b029447b900df216cb76ff39
9 Nbf60986608f6486397760410b7a39010
10 Nc9172b32f27140b18d6ec88bd63f4172
11 Ncc7336ac0d924e3eba2e9c074b83db14
12 Necab2225b84248fe9d5e959a10715906
13 anzsrc-for:11
14 anzsrc-for:1103
15 schema:author N39aa4b8efde14906aacc1faaa0ab3aac
16 schema:citation sg:pub.10.1007/s001130050443
17 sg:pub.10.1038/sj.gt.3301772
18 https://app.dimensions.ai/details/publication/pub.1082647163
19 https://app.dimensions.ai/details/publication/pub.1082950642
20 https://doi.org/10.1002/jor.1100140115
21 https://doi.org/10.1002/jor.1100180613
22 https://doi.org/10.1006/mthe.2002.0654
23 https://doi.org/10.1016/1357-4310(96)88792-2
24 https://doi.org/10.1016/s1357-4310(98)01231-3
25 https://doi.org/10.1016/s8756-3282(97)00074-4
26 https://doi.org/10.1056/nejm199512073332308
27 https://doi.org/10.1083/jcb.142.5.1257
28 https://doi.org/10.1089/10430340050015248
29 https://doi.org/10.1089/104303402320138989
30 https://doi.org/10.1097/00003086-200009000-00040
31 https://doi.org/10.1097/00003086-200210001-00028
32 https://doi.org/10.1097/00007632-199609150-00012
33 https://doi.org/10.1126/science.150.3698.893
34 https://doi.org/10.1172/jci15153
35 https://doi.org/10.2106/00004623-198062070-00001
36 https://doi.org/10.2106/00004623-199907000-00002
37 https://doi.org/10.2106/00004623-200107000-00008
38 schema:datePublished 2003-10
39 schema:datePublishedReg 2003-10-01
40 schema:description BACKGROUND: The ideal cellular vehicle for use in cell-mediated gene therapy to enhance bone healing has not yet been identified. The purpose of this study was to compare the capacity of two types of cells transduced with retro-bone morphogenetic protein 4 (BMP4)-muscle-derived cells (MDCs) and unfractioned bone marrow stromal cells (BMSCs). METHOD: Primary rat MDCs and unfractioned rat BMSCs were transduced with a retrovirus to express BMP4. A 7-mm, critical-sized femur defect was created in adult rats, and 5 x 10(6) transduced cells were implanted into the femoral defect. Bone healing was monitored radiographically and histologically at 4, 8, and 12 weeks post-implantation. RESULTS: All specimens in the MDC-BMP4 group and BMSC-BMP4 group showed a bridging callus at 8 and 12 weeks. At 12 weeks post-implantation the calluses of the MDC-BMP4 femora displayed significantly higher bone photodensity than the BMSC-BMP4 femora (P<0.05). Histomorphometry revealed no difference between the two treatment groups. However, non-union between newly formed and original bone was observed in none of the MDC femora but in six femora from the BMSC-BMP4 group. CONCLUSION: Both MDCs and unfractioned BMSCs can improve healing of a critical-sized bone defect following transduction of the cells with retroBMP4. However, MDCs appear to yield superior results when compared with BMSCs in terms of improved healing of segmental defects.
41 schema:genre research_article
42 schema:inLanguage en
43 schema:isAccessibleForFree false
44 schema:isPartOf Nf43467dba66342c6b026c99934b1aff0
45 Nfa273fcb6b8642c3a15037353c8ebd0f
46 sg:journal.1295031
47 schema:name The role of cell type in bone healing mediated by ex vivo gene therapy
48 schema:pagination 347-355
49 schema:productId N35f35465391a496291af23abece0e263
50 N3a2c5a1b40b0411d872abe39dddbc8b7
51 Na11cfd57975a404283ebfd1b1a0860ea
52 Nc996e0254e52413abf86ad58a43d40b3
53 Nd586c891d15f498fa4b5a067d0ab1fab
54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038324499
55 https://doi.org/10.1007/s00423-003-0401-7
56 schema:sdDatePublished 2019-04-10T22:27
57 schema:sdLicense https://scigraph.springernature.com/explorer/license/
58 schema:sdPublisher Nd84c34d2b9944cd69fb9df7792ee452d
59 schema:url http://link.springer.com/10.1007/s00423-003-0401-7
60 sgo:license sg:explorer/license/
61 sgo:sdDataset articles
62 rdf:type schema:ScholarlyArticle
63 N2dd3388a499f41fa95fe837be1a4408a rdf:first sg:person.01317744350.94
64 rdf:rest Nea320120cf7d4da99109a0db8be07a4b
65 N35f35465391a496291af23abece0e263 schema:name pubmed_id
66 schema:value 14534793
67 rdf:type schema:PropertyValue
68 N39aa4b8efde14906aacc1faaa0ab3aac rdf:first sg:person.01236371200.17
69 rdf:rest N2dd3388a499f41fa95fe837be1a4408a
70 N3a2c5a1b40b0411d872abe39dddbc8b7 schema:name nlm_unique_id
71 schema:value 9808285
72 rdf:type schema:PropertyValue
73 N3d020c327aaa416fa129ed042dc230a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Male
75 rdf:type schema:DefinedTerm
76 N451c5f44ae0447389fceb85447ec2836 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Stromal Cells
78 rdf:type schema:DefinedTerm
79 N543db2846373439288d292fb6f4e0a7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Rats, Inbred F344
81 rdf:type schema:DefinedTerm
82 N6507daf30cb648f8838362aa2d765630 rdf:first sg:person.01025022257.04
83 rdf:rest Nc596f36ea4b9407689a7f0fdf1186920
84 N6fc4115364f0490088c94187d25c6219 rdf:first sg:person.0706577466.24
85 rdf:rest Ncaf5868b574841388150722b51c254b6
86 N8298c72354f243e6abfc602f89c03b40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Bone Morphogenetic Protein 4
88 rdf:type schema:DefinedTerm
89 N82a4cb133be645bf9f832a5dd8c63940 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Animals
91 rdf:type schema:DefinedTerm
92 N8b2a660c81f44b7d9539b3c6302de6c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Wound Healing
94 rdf:type schema:DefinedTerm
95 N9dd1d4e60e3748cfb9a58e8b5413a011 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Rats
97 rdf:type schema:DefinedTerm
98 Na11cfd57975a404283ebfd1b1a0860ea schema:name doi
99 schema:value 10.1007/s00423-003-0401-7
100 rdf:type schema:PropertyValue
101 Na7d5dd94b029447b900df216cb76ff39 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Genetic Therapy
103 rdf:type schema:DefinedTerm
104 Nbf60986608f6486397760410b7a39010 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Bone Morphogenetic Proteins
106 rdf:type schema:DefinedTerm
107 Nc596f36ea4b9407689a7f0fdf1186920 rdf:first sg:person.01034641540.20
108 rdf:rest N6fc4115364f0490088c94187d25c6219
109 Nc9172b32f27140b18d6ec88bd63f4172 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Transduction, Genetic
111 rdf:type schema:DefinedTerm
112 Nc996e0254e52413abf86ad58a43d40b3 schema:name dimensions_id
113 schema:value pub.1038324499
114 rdf:type schema:PropertyValue
115 Ncaf5868b574841388150722b51c254b6 rdf:first sg:person.0603751545.18
116 rdf:rest rdf:nil
117 Ncc7336ac0d924e3eba2e9c074b83db14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Bone Marrow Cells
119 rdf:type schema:DefinedTerm
120 Nd586c891d15f498fa4b5a067d0ab1fab schema:name readcube_id
121 schema:value b69042a0921e1222da37e475721c1991dd321178806c5e5934270fd180fd0edc
122 rdf:type schema:PropertyValue
123 Nd84c34d2b9944cd69fb9df7792ee452d schema:name Springer Nature - SN SciGraph project
124 rdf:type schema:Organization
125 Nea320120cf7d4da99109a0db8be07a4b rdf:first sg:person.0742403260.89
126 rdf:rest Nf8e91e9cdc98416e8ae6c751022022cc
127 Necab2225b84248fe9d5e959a10715906 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Chondrocytes
129 rdf:type schema:DefinedTerm
130 Nf43467dba66342c6b026c99934b1aff0 schema:issueNumber 5
131 rdf:type schema:PublicationIssue
132 Nf8e91e9cdc98416e8ae6c751022022cc rdf:first sg:person.0777360524.84
133 rdf:rest N6507daf30cb648f8838362aa2d765630
134 Nfa273fcb6b8642c3a15037353c8ebd0f schema:volumeNumber 388
135 rdf:type schema:PublicationVolume
136 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
137 schema:name Medical and Health Sciences
138 rdf:type schema:DefinedTerm
139 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
140 schema:name Clinical Sciences
141 rdf:type schema:DefinedTerm
142 sg:journal.1295031 schema:issn 1435-2443
143 1435-2451
144 schema:name Langenbeck's Archives of Surgery
145 rdf:type schema:Periodical
146 sg:person.01025022257.04 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
147 schema:familyName Kuroda
148 schema:givenName Ryosuke
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025022257.04
150 rdf:type schema:Person
151 sg:person.01034641540.20 schema:affiliation https://www.grid.ac/institutes/grid.9647.c
152 schema:familyName Lill
153 schema:givenName Helmut
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034641540.20
155 rdf:type schema:Person
156 sg:person.01236371200.17 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
157 schema:familyName Rose
158 schema:givenName Tim
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236371200.17
160 rdf:type schema:Person
161 sg:person.01317744350.94 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
162 schema:familyName Peng
163 schema:givenName Hairong
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317744350.94
165 rdf:type schema:Person
166 sg:person.0603751545.18 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
167 schema:familyName Huard
168 schema:givenName Johnny
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603751545.18
170 rdf:type schema:Person
171 sg:person.0706577466.24 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
172 schema:familyName Fu
173 schema:givenName Freddie H.
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706577466.24
175 rdf:type schema:Person
176 sg:person.0742403260.89 schema:affiliation https://www.grid.ac/institutes/grid.278244.f
177 schema:familyName Shen
178 schema:givenName Hsain-Chung
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742403260.89
180 rdf:type schema:Person
181 sg:person.0777360524.84 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
182 schema:familyName Usas
183 schema:givenName Arvydas
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777360524.84
185 rdf:type schema:Person
186 sg:pub.10.1007/s001130050443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004578554
187 https://doi.org/10.1007/s001130050443
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/sj.gt.3301772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031454600
190 https://doi.org/10.1038/sj.gt.3301772
191 rdf:type schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1082647163 schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1082950642 schema:CreativeWork
194 https://doi.org/10.1002/jor.1100140115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026460241
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1002/jor.1100180613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012800545
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1006/mthe.2002.0654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028433647
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/1357-4310(96)88792-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001332045
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/s1357-4310(98)01231-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022007968
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/s8756-3282(97)00074-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041183246
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1056/nejm199512073332308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024758674
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1083/jcb.142.5.1257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007731134
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1089/10430340050015248 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059201901
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1089/104303402320138989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059202336
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1097/00003086-200009000-00040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060168389
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1097/00003086-200210001-00028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060169430
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1097/00007632-199609150-00012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003885165
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1126/science.150.3698.893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062487133
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1172/jci15153 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035029632
223 rdf:type schema:CreativeWork
224 https://doi.org/10.2106/00004623-198062070-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068887424
225 rdf:type schema:CreativeWork
226 https://doi.org/10.2106/00004623-199907000-00002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068893402
227 rdf:type schema:CreativeWork
228 https://doi.org/10.2106/00004623-200107000-00008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068894485
229 rdf:type schema:CreativeWork
230 https://www.grid.ac/institutes/grid.21925.3d schema:alternateName University of Pittsburgh
231 schema:name Department of Orthopedics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
232 Department of Trauma and Reconstructive Surgery, University of Leipzig, Liebigstrasse 20a, 04103 Leipzig, Germany
233 Growth and Development Laboratory, Department of Orthopedic Surgery, Children’s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA
234 rdf:type schema:Organization
235 https://www.grid.ac/institutes/grid.278244.f schema:alternateName Tri-Service General Hospital
236 schema:name Department of Orthopedic Surgery, Tri-Service General Hospital, Taipei, Taiwan
237 Growth and Development Laboratory, Department of Orthopedic Surgery, Children’s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA
238 rdf:type schema:Organization
239 https://www.grid.ac/institutes/grid.9647.c schema:alternateName Leipzig University
240 schema:name Department of Trauma and Reconstructive Surgery, University of Leipzig, Liebigstrasse 20a, 04103 Leipzig, Germany
241 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...