Embryonic gene expression resolved at the cellular level by fluorescence in situ hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-06

AUTHORS

Christian Paratore, Ueli Suter, Lukas Sommer

ABSTRACT

Tyramide signal amplification has successfully been applied to enhance detection limits of both immunological reactions and in situ hybridization methods. The technique uses short-range deposition of activated tyramide mediated by horseradish peroxidase. We have adapted this method to fluorescence in situ hybridization on embryonic tissue sections using fluorophore-labeled tyramide. The sensitivity of the procedure was sufficient to analyze the embryonic expression of mRNAs encoding both transcription factors and structural proteins. Combining fluorescence in situ hybridization and immunofluorescence with confocal microscopy allows the simultaneous detection of distinct mRNA species or of mRNAs together with proteins on the cellular level. Thus, the cell types expressing a particular gene at a given developmental stage can be studied even if no antibody to the gene product of interest is available. Moreover, the technique allows to study in situ the combinatorial marker expression that characterizes progenitor stages of a given cell lineage. More... »

PAGES

435-443

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s004180050379

DOI

http://dx.doi.org/10.1007/s004180050379

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050813961

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10429965


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbocyanines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic and Fetal Development", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescein-5-isothiocyanate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Dyes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lung", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sensitivity and Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland e-mail: sommer@cell.biol.ethz.ch Tel.: +41-1-6333349, Fax: +41-1-6331190, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Paratore", 
        "givenName": "Christian", 
        "id": "sg:person.0766553730.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766553730.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland e-mail: sommer@cell.biol.ethz.ch Tel.: +41-1-6333349, Fax: +41-1-6331190, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suter", 
        "givenName": "Ueli", 
        "id": "sg:person.01176472422.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176472422.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland e-mail: sommer@cell.biol.ethz.ch Tel.: +41-1-6333349, Fax: +41-1-6331190, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sommer", 
        "givenName": "Lukas", 
        "id": "sg:person.01110510133.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110510133.31"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1999-06", 
    "datePublishedReg": "1999-06-01", 
    "description": "Tyramide signal amplification has successfully been applied to enhance detection limits of both immunological reactions and in situ hybridization methods. The technique uses short-range deposition of activated tyramide mediated by horseradish peroxidase. We have adapted this method to fluorescence in situ hybridization on embryonic tissue sections using fluorophore-labeled tyramide. The sensitivity of the procedure was sufficient to analyze the embryonic expression of mRNAs encoding both transcription factors and structural proteins. Combining fluorescence in situ hybridization and immunofluorescence with confocal microscopy allows the simultaneous detection of distinct mRNA species or of mRNAs together with proteins on the cellular level. Thus, the cell types expressing a particular gene at a given developmental stage can be studied even if no antibody to the gene product of interest is available. Moreover, the technique allows to study in situ the combinatorial marker expression that characterizes progenitor stages of a given cell lineage.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s004180050379", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1294817", 
        "issn": [
          "0948-6143", 
          "1432-119X"
        ], 
        "name": "Histochemistry and Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "111"
      }
    ], 
    "name": "Embryonic gene expression resolved at the cellular level by fluorescence in situ hybridization", 
    "pagination": "435-443", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f20eba1c6f4d73643e7cba3c752eee9d60de843f4add3d8592d6be4cfaecb2b9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10429965"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9506663"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s004180050379"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1050813961"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s004180050379", 
      "https://app.dimensions.ai/details/publication/pub.1050813961"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000516.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs004180050379"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s004180050379'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s004180050379'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s004180050379'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s004180050379'


 

This table displays all metadata directly associated to this object as RDF triples.

131 TRIPLES      20 PREDICATES      41 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s004180050379 schema:about N11cdc7f412514c5fa1ab98042e7b13fb
2 N15e37bbd2d7348bd918fe1d414289943
3 N161dee389ed741eea2bd8664441e57c0
4 N189041a94aab46da9b61b0d2759db93f
5 N1ecae8b8f0484a3b9a4835f1e3062f55
6 N2a80827fec0b4df9aa940a4613452a6c
7 N2ac657b96d6e460695427a10afb8fdcc
8 N5ec4d8682dce4aba9d58877f6aa7765e
9 N64a8515def0e492f85f32b0e6d58bdfd
10 N737ddee0c644405ca10e992a75646d1e
11 N813bbeed18814774861b59f9bab36cd2
12 Nd5e9025707514d9ebaa53e7977bd21e3
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author Nffa4004deecc497299f40fb8d4364114
16 schema:datePublished 1999-06
17 schema:datePublishedReg 1999-06-01
18 schema:description Tyramide signal amplification has successfully been applied to enhance detection limits of both immunological reactions and in situ hybridization methods. The technique uses short-range deposition of activated tyramide mediated by horseradish peroxidase. We have adapted this method to fluorescence in situ hybridization on embryonic tissue sections using fluorophore-labeled tyramide. The sensitivity of the procedure was sufficient to analyze the embryonic expression of mRNAs encoding both transcription factors and structural proteins. Combining fluorescence in situ hybridization and immunofluorescence with confocal microscopy allows the simultaneous detection of distinct mRNA species or of mRNAs together with proteins on the cellular level. Thus, the cell types expressing a particular gene at a given developmental stage can be studied even if no antibody to the gene product of interest is available. Moreover, the technique allows to study in situ the combinatorial marker expression that characterizes progenitor stages of a given cell lineage.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N371d895ac1c841f298c5c6b872adb74f
23 N5284cbcc0a7e4239854f2480ad6dae45
24 sg:journal.1294817
25 schema:name Embryonic gene expression resolved at the cellular level by fluorescence in situ hybridization
26 schema:pagination 435-443
27 schema:productId N04fefd988c7e4df0b3cc22386d448ef9
28 N0c2e07a2419645a1a9d2ae066eb9824c
29 N62e3a35a81b845e098a9041ccfbd327c
30 N6dd5e019d3a841548aaa36889377d6c8
31 Na08b5433dfe84b2babc73b7ebe1bea2b
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050813961
33 https://doi.org/10.1007/s004180050379
34 schema:sdDatePublished 2019-04-11T02:03
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher N9574fc73de0a4849b038f4f062e7f0e3
37 schema:url http://link.springer.com/10.1007%2Fs004180050379
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N04fefd988c7e4df0b3cc22386d448ef9 schema:name nlm_unique_id
42 schema:value 9506663
43 rdf:type schema:PropertyValue
44 N0c2e07a2419645a1a9d2ae066eb9824c schema:name readcube_id
45 schema:value f20eba1c6f4d73643e7cba3c752eee9d60de843f4add3d8592d6be4cfaecb2b9
46 rdf:type schema:PropertyValue
47 N11cdc7f412514c5fa1ab98042e7b13fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Gene Expression
49 rdf:type schema:DefinedTerm
50 N15e37bbd2d7348bd918fe1d414289943 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name In Situ Hybridization, Fluorescence
52 rdf:type schema:DefinedTerm
53 N161dee389ed741eea2bd8664441e57c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Animals
55 rdf:type schema:DefinedTerm
56 N189041a94aab46da9b61b0d2759db93f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
57 schema:name Embryonic and Fetal Development
58 rdf:type schema:DefinedTerm
59 N1ecae8b8f0484a3b9a4835f1e3062f55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Mice
61 rdf:type schema:DefinedTerm
62 N2a80827fec0b4df9aa940a4613452a6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name Sensitivity and Specificity
64 rdf:type schema:DefinedTerm
65 N2ac657b96d6e460695427a10afb8fdcc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name DNA-Binding Proteins
67 rdf:type schema:DefinedTerm
68 N371d895ac1c841f298c5c6b872adb74f schema:issueNumber 6
69 rdf:type schema:PublicationIssue
70 N5284cbcc0a7e4239854f2480ad6dae45 schema:volumeNumber 111
71 rdf:type schema:PublicationVolume
72 N5ec4d8682dce4aba9d58877f6aa7765e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Lung
74 rdf:type schema:DefinedTerm
75 N62e3a35a81b845e098a9041ccfbd327c schema:name dimensions_id
76 schema:value pub.1050813961
77 rdf:type schema:PropertyValue
78 N64a8515def0e492f85f32b0e6d58bdfd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Fluorescein-5-isothiocyanate
80 rdf:type schema:DefinedTerm
81 N6dd5e019d3a841548aaa36889377d6c8 schema:name doi
82 schema:value 10.1007/s004180050379
83 rdf:type schema:PropertyValue
84 N737ddee0c644405ca10e992a75646d1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Fluorescent Dyes
86 rdf:type schema:DefinedTerm
87 N771b9a454672431aa937939e595eeaf6 rdf:first sg:person.01110510133.31
88 rdf:rest rdf:nil
89 N813bbeed18814774861b59f9bab36cd2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Transcription Factors
91 rdf:type schema:DefinedTerm
92 N9574fc73de0a4849b038f4f062e7f0e3 schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 Na08b5433dfe84b2babc73b7ebe1bea2b schema:name pubmed_id
95 schema:value 10429965
96 rdf:type schema:PropertyValue
97 Nc6748e04e6e049ac8ef1114e1ee157f3 rdf:first sg:person.01176472422.00
98 rdf:rest N771b9a454672431aa937939e595eeaf6
99 Nd5e9025707514d9ebaa53e7977bd21e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Carbocyanines
101 rdf:type schema:DefinedTerm
102 Nffa4004deecc497299f40fb8d4364114 rdf:first sg:person.0766553730.62
103 rdf:rest Nc6748e04e6e049ac8ef1114e1ee157f3
104 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
105 schema:name Biological Sciences
106 rdf:type schema:DefinedTerm
107 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
108 schema:name Biochemistry and Cell Biology
109 rdf:type schema:DefinedTerm
110 sg:journal.1294817 schema:issn 0948-6143
111 1432-119X
112 schema:name Histochemistry and Cell Biology
113 rdf:type schema:Periodical
114 sg:person.01110510133.31 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
115 schema:familyName Sommer
116 schema:givenName Lukas
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110510133.31
118 rdf:type schema:Person
119 sg:person.01176472422.00 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
120 schema:familyName Suter
121 schema:givenName Ueli
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176472422.00
123 rdf:type schema:Person
124 sg:person.0766553730.62 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
125 schema:familyName Paratore
126 schema:givenName Christian
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766553730.62
128 rdf:type schema:Person
129 https://www.grid.ac/institutes/grid.5801.c schema:alternateName Swiss Federal Institute of Technology in Zurich
130 schema:name Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland e-mail: sommer@cell.biol.ethz.ch Tel.: +41-1-6333349, Fax: +41-1-6331190, CH
131 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...