Identification of markers for quiescent pancreatic stellate cells in the normal human pancreas View Full Text


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Article Info

DATE

2017-05-25

AUTHORS

Michael Friberg Bruun Nielsen, Michael Bau Mortensen, Sönke Detlefsen

ABSTRACT

Pancreatic stellate cells (PSCs) play a central role as source of fibrogenic cells in pancreatic cancer and chronic pancreatitis. In contrast to quiescent hepatic stellate cells (qHSCs), a specific marker for quiescent PSCs (qPSCs) that can be used in formalin-fixed and paraffin embedded (FFPE) normal human pancreatic tissue has not been identified. The aim of this study was to identify a marker enabling the identification of qPSCs in normal human FFPE pancreatic tissue. Immunohistochemical (IHC), double-IHC, immunofluorescence (IF) and double-IF analyses were carried out using a tissue microarray consisting of cores with normal human pancreatic tissue. Cores with normal human liver served as control. Antibodies directed against adipophilin, α-SMA, CD146, CRBP-1, cytoglobin, desmin, GFAP, nestin, S100A4 and vinculin were examined, with special emphasis on their expression in periacinar cells in the normal human pancreas and perisinusoidal cells in the normal human liver. The immunolabelling capacity was evaluated according to a semiquantitative scoring system. Double-IF of the markers of interest together with markers for other periacinar cells was performed. Moreover, the utility of histochemical stains for the identification of human qPSCs was examined, and their ultrastructure was revisited by electron microscopy. Adipophilin, CRBP-1, cytoglobin and vinculin were expressed in qHSCs in the liver, whereas cytoglobin and adipophilin were expressed in qPSCs in the pancreas. Adipophilin immunohistochemistry was highly dependent on the preanalytical time interval (PATI) from removal of the tissue to formalin fixation. Cytoglobin, S100A4 and vinculin were expressed in periacinar fibroblasts (FBs). The other examined markers were negative in human qPSCs. Our data indicate that cytoglobin and adipophilin are markers of qPSCs in the normal human pancreas. However, the use of adipophilin as a qPSC marker may be limited due to its high dependence on optimal PATI. Cytoglobin, on the other hand, is a sensitive marker for qPSCs but is expressed in FBs as well. More... »

PAGES

359-380

References to SciGraph publications

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  • 2007-02-09. The diffuse stellate cell system in JOURNAL OF MOLECULAR HISTOLOGY
  • 2004-05-08. Fibrosis of the pancreas: the initial tissue damage and the resulting pattern in VIRCHOWS ARCHIV
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  • 2005-07-14. Pancreatic fibrosis associated with age and ductal papillary hyperplasia in VIRCHOWS ARCHIV
  • 2013-12-02. Cytoglobin is expressed in hepatic stellate cells, but not in myofibroblasts, in normal and fibrotic human liver in LABORATORY INVESTIGATION
  • 2008-12-04. Vinculin and cellular retinol-binding protein-1 are markers for quiescent and activated hepatic stellate cells in formalin-fixed paraffin embedded human liver in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2006-05-05. Fibrogenesis in alcoholic chronic pancreatitis: the role of tissue necrosis, macrophages, myofibroblasts and cytokines in MODERN PATHOLOGY
  • 1993-08. Intralobular heterogeneity of perisinusoidal stellate cells in porcine liver in CELL AND TISSUE RESEARCH
  • 2002-05-14. Nestin expression in pancreatic stellate cells and angiogenic endothelial cells in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2003-12-24. News from the Managing Editors, 2003–2004 in VIRCHOWS ARCHIV
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    http://scigraph.springernature.com/pub.10.1007/s00418-017-1581-5

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    DIMENSIONS

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    PUBMED

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