Visualization of tumor heterogeneity by in situ padlock probe technology in colorectal cancer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-08

AUTHORS

Amin El-Heliebi, Karl Kashofer, Julia Fuchs, Stephan W. Jahn, Christian Viertler, Andrija Matak, Peter Sedlmayr, Gerald Hoefler

ABSTRACT

Tumor heterogeneity is considered a major cause for therapy resistance in colorectal cancer. Sub-populations of cells with different genetic alterations may exist in spatially distinct areas. Upon therapy, resistant sub-clones may enrich and ultimately lead to disease progression. Although ample data are available on tumors which are heterogeneous on a morphological level, only little is known about morphologically homogeneous tumors. We aimed to investigate if morphologically homogeneous colorectal cancer can harbor a heterogeneous genetic landscape. We chose to microdissect six morphologically homogeneous colorectal carcinomas into several areas and performed next-generation sequencing (NGS) to identify tumors with genetic heterogeneity. We then applied an mRNA-based in situ mutation detection technology based on padlock probes to localize and visualize mutations directly in the tumor tissue. In three out of six tumors, NGS revealed a high rate of variability of mutations between different tumor areas. We selected two cases for in situ mutation detection to visualize genetic heterogeneity. In situ mutation detection confirmed differences in mutant allele frequencies between different tumor areas of morphological homogeneous tumors. We conclude that genetic heterogeneity in morphologically homogeneous colorectal cancer is an observable, but underreported event. Our results illustrate the power of in situ mutation analysis to visualize genetic heterogeneity directly in tumor tissue. More... »

PAGES

105-115

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00418-017-1557-5

DOI

http://dx.doi.org/10.1007/s00418-017-1557-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084504545

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28321501


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colorectal Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Mutational Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Heterogeneity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Point Mutation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/VII, 8010, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "El-Heliebi", 
        "givenName": "Amin", 
        "id": "sg:person.0677437314.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677437314.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Pathology, Medical University of Graz, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kashofer", 
        "givenName": "Karl", 
        "id": "sg:person.0611223507.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611223507.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/VII, 8010, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fuchs", 
        "givenName": "Julia", 
        "id": "sg:person.016101755363.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016101755363.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Pathology, Medical University of Graz, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jahn", 
        "givenName": "Stephan W.", 
        "id": "sg:person.0674014371.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674014371.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Pathology, Medical University of Graz, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Viertler", 
        "givenName": "Christian", 
        "id": "sg:person.01220401207.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220401207.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Pathology, Medical University of Graz, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matak", 
        "givenName": "Andrija", 
        "id": "sg:person.01112176445.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112176445.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/VII, 8010, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sedlmayr", 
        "givenName": "Peter", 
        "id": "sg:person.01277005030.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277005030.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medical University of Graz", 
          "id": "https://www.grid.ac/institutes/grid.11598.34", 
          "name": [
            "Institute of Pathology, Medical University of Graz, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hoefler", 
        "givenName": "Gerald", 
        "id": "sg:person.0755631152.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755631152.57"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.3390/ijms160613610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000003725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007480955", 
          "https://doi.org/10.1038/nature10242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng.3214", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008423455", 
          "https://doi.org/10.1038/ng.3214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-09-1022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008547804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature11252", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010619765", 
          "https://doi.org/10.1038/nature11252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.3252", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012522947", 
          "https://doi.org/10.1038/nsmb.3252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.04.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012547698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/ijms13021951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014716916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2013.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015048246", 
          "https://doi.org/10.1038/nprot.2013.006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.1527", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015865863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1256930", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018285655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1256930", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018285655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.2015.131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019247758", 
          "https://doi.org/10.1038/labinvest.2015.131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1754", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019307928", 
          "https://doi.org/10.1038/nbt.1754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.4056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019985327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020792304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature11156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024120060", 
          "https://doi.org/10.1038/nature11156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-12-4140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024514458"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature12625", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024549098", 
          "https://doi.org/10.1038/nature12625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja020778f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024554177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja020778f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024554177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026380621", 
          "https://doi.org/10.1038/nmeth.1448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026380621", 
          "https://doi.org/10.1038/nmeth.1448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature12627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028310096", 
          "https://doi.org/10.1038/nature12627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1113205", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031166230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2353/jmoldx.2008.070082", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031898520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.2015.159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039467132", 
          "https://doi.org/10.1038/labinvest.2015.159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc3261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039488853", 
          "https://doi.org/10.1038/nrc3261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0087663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043695054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.04.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045709558"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1253462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046584781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/fly.19695", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046734556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1260419", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048221082"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/2159-8290.cd-14-0462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049613996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3978/j.issn.2078-6891.2012.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078616367"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-08", 
    "datePublishedReg": "2017-08-01", 
    "description": "Tumor heterogeneity is considered a major cause for therapy resistance in colorectal cancer. Sub-populations of cells with different genetic alterations may exist in spatially distinct areas. Upon therapy, resistant sub-clones may enrich and ultimately lead to disease progression. Although ample data are available on tumors which are heterogeneous on a morphological level, only little is known about morphologically homogeneous tumors. We aimed to investigate if morphologically homogeneous colorectal cancer can harbor a heterogeneous genetic landscape. We chose to microdissect six morphologically homogeneous colorectal carcinomas into several areas and performed next-generation sequencing (NGS) to identify tumors with genetic heterogeneity. We then applied an mRNA-based in situ mutation detection technology based on padlock probes to localize and visualize mutations directly in the tumor tissue. In three out of six tumors, NGS revealed a high rate of variability of mutations between different tumor areas. We selected two cases for in situ mutation detection to visualize genetic heterogeneity. In situ mutation detection confirmed differences in mutant allele frequencies between different tumor areas of morphological homogeneous tumors. We conclude that genetic heterogeneity in morphologically homogeneous colorectal cancer is an observable, but underreported event. Our results illustrate the power of in situ mutation analysis to visualize genetic heterogeneity directly in tumor tissue.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00418-017-1557-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1294817", 
        "issn": [
          "0948-6143", 
          "1432-119X"
        ], 
        "name": "Histochemistry and Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "148"
      }
    ], 
    "name": "Visualization of tumor heterogeneity by in situ padlock probe technology in colorectal cancer", 
    "pagination": "105-115", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "fc2bf737144711fd0fd6b0882a3eab06a3da125034a83c7cf5261078aaf3e03c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28321501"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9506663"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00418-017-1557-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084504545"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00418-017-1557-5", 
      "https://app.dimensions.ai/details/publication/pub.1084504545"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113670_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00418-017-1557-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00418-017-1557-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00418-017-1557-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00418-017-1557-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00418-017-1557-5'


 

This table displays all metadata directly associated to this object as RDF triples.

252 TRIPLES      21 PREDICATES      67 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00418-017-1557-5 schema:about N430de600eccd44f2a71d19f54480cc52
2 N47e57224e98347ddafdbd937dfc92172
3 N581e00512c5b4dd3b576dcd67d36a759
4 N629274f807d54236a3abcf293d68f3cd
5 Nb6ad39c361e645b9bc170632cb721e12
6 Nf6db6034b2d945ec8da86c621c1e97f0
7 anzsrc-for:11
8 anzsrc-for:1112
9 schema:author N231e230ad2b94b99b73cc9368ebaaaf1
10 schema:citation sg:pub.10.1038/labinvest.2015.131
11 sg:pub.10.1038/labinvest.2015.159
12 sg:pub.10.1038/nature10242
13 sg:pub.10.1038/nature11156
14 sg:pub.10.1038/nature11252
15 sg:pub.10.1038/nature12625
16 sg:pub.10.1038/nature12627
17 sg:pub.10.1038/nbt.1754
18 sg:pub.10.1038/ng.3214
19 sg:pub.10.1038/nmeth.1448
20 sg:pub.10.1038/nprot.2013.006
21 sg:pub.10.1038/nrc3261
22 sg:pub.10.1038/nsmb.3252
23 https://doi.org/10.1016/j.cell.2012.04.023
24 https://doi.org/10.1016/j.cell.2012.04.024
25 https://doi.org/10.1021/ja020778f
26 https://doi.org/10.1056/nejmoa1113205
27 https://doi.org/10.1093/nar/gkq603
28 https://doi.org/10.1126/science.1253462
29 https://doi.org/10.1126/science.1256930
30 https://doi.org/10.1126/science.1260419
31 https://doi.org/10.1158/0008-5472.can-09-1022
32 https://doi.org/10.1158/0008-5472.can-12-4140
33 https://doi.org/10.1158/2159-8290.cd-14-0462
34 https://doi.org/10.1371/journal.pone.0087663
35 https://doi.org/10.18632/oncotarget.1527
36 https://doi.org/10.18632/oncotarget.4056
37 https://doi.org/10.2353/jmoldx.2008.070082
38 https://doi.org/10.3390/ijms13021951
39 https://doi.org/10.3390/ijms160613610
40 https://doi.org/10.3978/j.issn.2078-6891.2012.030
41 https://doi.org/10.4161/fly.19695
42 schema:datePublished 2017-08
43 schema:datePublishedReg 2017-08-01
44 schema:description Tumor heterogeneity is considered a major cause for therapy resistance in colorectal cancer. Sub-populations of cells with different genetic alterations may exist in spatially distinct areas. Upon therapy, resistant sub-clones may enrich and ultimately lead to disease progression. Although ample data are available on tumors which are heterogeneous on a morphological level, only little is known about morphologically homogeneous tumors. We aimed to investigate if morphologically homogeneous colorectal cancer can harbor a heterogeneous genetic landscape. We chose to microdissect six morphologically homogeneous colorectal carcinomas into several areas and performed next-generation sequencing (NGS) to identify tumors with genetic heterogeneity. We then applied an mRNA-based in situ mutation detection technology based on padlock probes to localize and visualize mutations directly in the tumor tissue. In three out of six tumors, NGS revealed a high rate of variability of mutations between different tumor areas. We selected two cases for in situ mutation detection to visualize genetic heterogeneity. In situ mutation detection confirmed differences in mutant allele frequencies between different tumor areas of morphological homogeneous tumors. We conclude that genetic heterogeneity in morphologically homogeneous colorectal cancer is an observable, but underreported event. Our results illustrate the power of in situ mutation analysis to visualize genetic heterogeneity directly in tumor tissue.
45 schema:genre research_article
46 schema:inLanguage en
47 schema:isAccessibleForFree true
48 schema:isPartOf N02f0aa67d3004fccb4a2830f2a0b70a5
49 Nec11a1933cce40158f3dc13bae1cec31
50 sg:journal.1294817
51 schema:name Visualization of tumor heterogeneity by in situ padlock probe technology in colorectal cancer
52 schema:pagination 105-115
53 schema:productId N062c233c14be434dbc32cf0755acea83
54 N3f4f16e780824596a8ce00d448ce0fc2
55 N87bf6708ef094f3f8b3f303b6d462eba
56 N88df947d7199462ebd41bcc8b8af6497
57 Nb88307f4adb5402cbcd27b316df8d3a2
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084504545
59 https://doi.org/10.1007/s00418-017-1557-5
60 schema:sdDatePublished 2019-04-11T10:36
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher N9f346a453aeb4faab47673a76b3d8cd7
63 schema:url https://link.springer.com/10.1007%2Fs00418-017-1557-5
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N02f0aa67d3004fccb4a2830f2a0b70a5 schema:volumeNumber 148
68 rdf:type schema:PublicationVolume
69 N062c233c14be434dbc32cf0755acea83 schema:name pubmed_id
70 schema:value 28321501
71 rdf:type schema:PropertyValue
72 N2237e4cb1cef47bd9a0a165ce552bdf6 rdf:first sg:person.01112176445.69
73 rdf:rest Nb8a32270611a4d9e9299ff0f4634a716
74 N231e230ad2b94b99b73cc9368ebaaaf1 rdf:first sg:person.0677437314.30
75 rdf:rest Nf404b37396da43e4a54269b71e6a21bf
76 N3f4f16e780824596a8ce00d448ce0fc2 schema:name readcube_id
77 schema:value fc2bf737144711fd0fd6b0882a3eab06a3da125034a83c7cf5261078aaf3e03c
78 rdf:type schema:PropertyValue
79 N430de600eccd44f2a71d19f54480cc52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Cell Line, Tumor
81 rdf:type schema:DefinedTerm
82 N47e57224e98347ddafdbd937dfc92172 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Humans
84 rdf:type schema:DefinedTerm
85 N581e00512c5b4dd3b576dcd67d36a759 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name DNA Mutational Analysis
87 rdf:type schema:DefinedTerm
88 N629274f807d54236a3abcf293d68f3cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Genetic Heterogeneity
90 rdf:type schema:DefinedTerm
91 N6512ac7ccf7548029c50bb8b7c856297 rdf:first sg:person.0755631152.57
92 rdf:rest rdf:nil
93 N6a69d51a4b81479dbf52cd6f551dcb2b rdf:first sg:person.0674014371.17
94 rdf:rest Nf0cce9167f6e44b7b7bca60c81382635
95 N87bf6708ef094f3f8b3f303b6d462eba schema:name nlm_unique_id
96 schema:value 9506663
97 rdf:type schema:PropertyValue
98 N88df947d7199462ebd41bcc8b8af6497 schema:name doi
99 schema:value 10.1007/s00418-017-1557-5
100 rdf:type schema:PropertyValue
101 N9f346a453aeb4faab47673a76b3d8cd7 schema:name Springer Nature - SN SciGraph project
102 rdf:type schema:Organization
103 Nb6ad39c361e645b9bc170632cb721e12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Point Mutation
105 rdf:type schema:DefinedTerm
106 Nb88307f4adb5402cbcd27b316df8d3a2 schema:name dimensions_id
107 schema:value pub.1084504545
108 rdf:type schema:PropertyValue
109 Nb8a32270611a4d9e9299ff0f4634a716 rdf:first sg:person.01277005030.51
110 rdf:rest N6512ac7ccf7548029c50bb8b7c856297
111 Nec11a1933cce40158f3dc13bae1cec31 schema:issueNumber 2
112 rdf:type schema:PublicationIssue
113 Nefa7265ca0184c6fab5dc469047fda3a rdf:first sg:person.016101755363.30
114 rdf:rest N6a69d51a4b81479dbf52cd6f551dcb2b
115 Nf0cce9167f6e44b7b7bca60c81382635 rdf:first sg:person.01220401207.65
116 rdf:rest N2237e4cb1cef47bd9a0a165ce552bdf6
117 Nf404b37396da43e4a54269b71e6a21bf rdf:first sg:person.0611223507.11
118 rdf:rest Nefa7265ca0184c6fab5dc469047fda3a
119 Nf6db6034b2d945ec8da86c621c1e97f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Colorectal Neoplasms
121 rdf:type schema:DefinedTerm
122 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
123 schema:name Medical and Health Sciences
124 rdf:type schema:DefinedTerm
125 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
126 schema:name Oncology and Carcinogenesis
127 rdf:type schema:DefinedTerm
128 sg:journal.1294817 schema:issn 0948-6143
129 1432-119X
130 schema:name Histochemistry and Cell Biology
131 rdf:type schema:Periodical
132 sg:person.01112176445.69 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
133 schema:familyName Matak
134 schema:givenName Andrija
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112176445.69
136 rdf:type schema:Person
137 sg:person.01220401207.65 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
138 schema:familyName Viertler
139 schema:givenName Christian
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220401207.65
141 rdf:type schema:Person
142 sg:person.01277005030.51 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
143 schema:familyName Sedlmayr
144 schema:givenName Peter
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277005030.51
146 rdf:type schema:Person
147 sg:person.016101755363.30 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
148 schema:familyName Fuchs
149 schema:givenName Julia
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016101755363.30
151 rdf:type schema:Person
152 sg:person.0611223507.11 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
153 schema:familyName Kashofer
154 schema:givenName Karl
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611223507.11
156 rdf:type schema:Person
157 sg:person.0674014371.17 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
158 schema:familyName Jahn
159 schema:givenName Stephan W.
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674014371.17
161 rdf:type schema:Person
162 sg:person.0677437314.30 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
163 schema:familyName El-Heliebi
164 schema:givenName Amin
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677437314.30
166 rdf:type schema:Person
167 sg:person.0755631152.57 schema:affiliation https://www.grid.ac/institutes/grid.11598.34
168 schema:familyName Hoefler
169 schema:givenName Gerald
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755631152.57
171 rdf:type schema:Person
172 sg:pub.10.1038/labinvest.2015.131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019247758
173 https://doi.org/10.1038/labinvest.2015.131
174 rdf:type schema:CreativeWork
175 sg:pub.10.1038/labinvest.2015.159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039467132
176 https://doi.org/10.1038/labinvest.2015.159
177 rdf:type schema:CreativeWork
178 sg:pub.10.1038/nature10242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007480955
179 https://doi.org/10.1038/nature10242
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/nature11156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024120060
182 https://doi.org/10.1038/nature11156
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/nature11252 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010619765
185 https://doi.org/10.1038/nature11252
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/nature12625 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024549098
188 https://doi.org/10.1038/nature12625
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/nature12627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028310096
191 https://doi.org/10.1038/nature12627
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/nbt.1754 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019307928
194 https://doi.org/10.1038/nbt.1754
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/ng.3214 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008423455
197 https://doi.org/10.1038/ng.3214
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/nmeth.1448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026380621
200 https://doi.org/10.1038/nmeth.1448
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nprot.2013.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015048246
203 https://doi.org/10.1038/nprot.2013.006
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nrc3261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039488853
206 https://doi.org/10.1038/nrc3261
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/nsmb.3252 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012522947
209 https://doi.org/10.1038/nsmb.3252
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/j.cell.2012.04.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012547698
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.cell.2012.04.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045709558
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1021/ja020778f schema:sameAs https://app.dimensions.ai/details/publication/pub.1024554177
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1056/nejmoa1113205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031166230
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1093/nar/gkq603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020792304
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1126/science.1253462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046584781
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1126/science.1256930 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018285655
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1126/science.1260419 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048221082
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1158/0008-5472.can-09-1022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008547804
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1158/0008-5472.can-12-4140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024514458
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1158/2159-8290.cd-14-0462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049613996
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1371/journal.pone.0087663 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043695054
234 rdf:type schema:CreativeWork
235 https://doi.org/10.18632/oncotarget.1527 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015865863
236 rdf:type schema:CreativeWork
237 https://doi.org/10.18632/oncotarget.4056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019985327
238 rdf:type schema:CreativeWork
239 https://doi.org/10.2353/jmoldx.2008.070082 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031898520
240 rdf:type schema:CreativeWork
241 https://doi.org/10.3390/ijms13021951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014716916
242 rdf:type schema:CreativeWork
243 https://doi.org/10.3390/ijms160613610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000003725
244 rdf:type schema:CreativeWork
245 https://doi.org/10.3978/j.issn.2078-6891.2012.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078616367
246 rdf:type schema:CreativeWork
247 https://doi.org/10.4161/fly.19695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046734556
248 rdf:type schema:CreativeWork
249 https://www.grid.ac/institutes/grid.11598.34 schema:alternateName Medical University of Graz
250 schema:name Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/VII, 8010, Graz, Austria
251 Institute of Pathology, Medical University of Graz, Graz, Austria
252 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...